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Navegação por Revista "Photodiagnosis and Photodynamic Therapy"
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SUZUKI, LUIS C.
; KATO, ILKA T.; PRATES, RENATO A.; SABINO, CAETANO P.
; YOSHIMURA, TANIA M.
; SILVA, TAMIRES O.; RIBEIRO, MARTHA S.
.
Glucose modulates antimicrobial photodynamic inactivation ofCandida albicans in biofilms.
Photodiagnosis and Photodynamic Therapy,
v. 17,
p. 173-179,
2017.
DOI:
10.1016/j.pdpdt.2016.12.003
Abstract:
Candida albicans biofilm is a main cause of infections associated with medical devices such as catheters,contact lens and artificial joint prosthesis. The current treatment comprises antifungal chemotherapy thatpresents low success rates. Photodynamic inactivation (PDI) involves the combination of a photosensitiz-ing compound (PS) and light to generate oxidative stress that has demonstrated effective antimicrobialactivity against a broad-spectrum of pathogens, including C. albicans. This fungus senses glucose induc-ing an upregulation of membrane transporters that can facilitate PS uptake into the cell. The aim ofthis study was to evaluate the effects of glucose on methylene blue (MB) uptake and its influence onPDI efficiency when combined to a red LED with central wavelength at = 660 nm. C. albicans biofilmswere grown on hydrogel disks. Prior to PDI assays, MB uptake tests were performed with and withoutglucose-sensitization. In this system, the optimum PS administration was determined as 500 M of MBin contact with the biofilm during 30 min before irradiation. Irradiation was performed during 3, 6, 9, 12,15 and 18 min with irradiance of 127.3 mW/cm2. Our results showed that glucose was able to increaseMB uptake in C. albicans cells. In addition, PDI without glucose showed a higher viability reduction until6 min; after 9 min, glucose group demonstrated a significant decrease in cell viability when compared toglucose-free group. Taken together, our data suggest that glucose is capable to enhance MB uptake andmodulate photodynamic inactivation of C. albicans biofilm.
Palavras-Chave:
glucose;
methylene blue;
infectious diseases;
inactivation;
photosensitivity;
yeasts
SUZUKI, LUIS C.; KATO, ILKA T.; PRATES, RENATO A.; SABINO, CAETANO P.; YOSHIMURA, TANIA M.; SILVA, TAMIRES O.; RIBEIRO, MARTHA S.
Glucose modulates antimicrobial photodynamic inactivation ofCandida albicans in biofilms.
Photodiagnosis and Photodynamic Therapy,
v. 17,
p. 173-179,
2017.
DOI:
10.1016/j.pdpdt.2016.12.003.
Disponível em: http://repositorio.ipen.br/handle/123456789/27375. Acesso em: $DATA.
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SELLERA, FABIO P.; SABINO, CAETANO P.
; RIBEIRO, MARTHA S.
; GARGANO, RONALDO G.; BENITES, NILSON R.; MELVILLE, PRISCILLA A.; POGLIANI, FABIO C..
In vitro photoinactivation of bovine mastitis related pathogens.
Photodiagnosis and Photodynamic Therapy,
v. 13,
p. 276-281,
2016.
Palavras-Chave:
in vitro;
cows;
therapy;
veterinary medicine;
diagnosis;
antibiotics;
pathogens;
cattle;
methylene blue
SELLERA, FABIO P.; SABINO, CAETANO P.; RIBEIRO, MARTHA S.; GARGANO, RONALDO G.; BENITES, NILSON R.; MELVILLE, PRISCILLA A.; POGLIANI, FABIO C.
In vitro photoinactivation of bovine mastitis related pathogens.
Photodiagnosis and Photodynamic Therapy,
v. 13,
p. 276-281,
2016.
Disponível em: http://repositorio.ipen.br/handle/123456789/26482. Acesso em: $DATA.
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SABINO, CAETANO P.; WAINWRIGHT, MARK; ANJOS, CAROLINA dos; SELLERA, FABIO P.; BAPTISTA, MAURICIO S.; LINCOPAN, NILTON; RIBEIRO, MARTHA S.
.
Inactivation kinetics and lethal dose analysis of antimicrobial blue light and photodynamic therapy.
Photodiagnosis and Photodynamic Therapy,
v. 28,
p. 186-191,
2019.
DOI:
10.1016/j.pdpdt.2019.08.022
Abstract:
Background: Antimicrobial Photodynamic therapy (A-PDT) has been used to treat infections. Currently, microbial
inactivation data is reported presenting survival fraction averages and standard errors as discrete points
instead of a continuous curve of inactivation kinetics. Standardization of this approach would allow clinical
protocols to be introduced globally, instead of the piecemeal situation which currently applies.
Methods: To this end, we used a power-law function to fit inactivation kinetics and directly report values of
lethal doses (LD) and a tolerance factor (T) that informs if inactivation rate varies along the irradiation procedure.
A deduced formula was also tested to predict LD for any given survival fraction value. We analyzed the
photoantimicrobial effect caused by red light activation of methylene blue (MB-APDT) and by blue light (BL)
activation of endogenous microbial pigments against 5 clinically relevant pathogens.
Results: Following MB- APDT, Escherichia coli and Staphylococcus aureus cells become increasingly more tolerant
to inactivation along the irradiation process (T < 1). Klebsiella pneumoniae presents opposite behavior, i.e., more
inactivation is observed towards the end of the process (T > 1). P. aeruginosa and Candida albicans present
constant inactivation rate (T˜1). In contrast, all bacterial species presented similar behavior during inactivation
caused by BL, i.e., continuously becoming more sensitive to blue light exposure (T > 1).
Conclusion: The power-law function successfully fit all experimental data. Our proposed method precisely
predicted LD and T values. We expect that these analytical models may contribute to more standardized methods
for comparisons of photodynamic inactivation efficiencies.
Palavras-Chave:
antimicrobial agents;
therapy;
chemotherapy;
inactivation;
lethal doses;
light sources;
data analysis;
candida;
methylene blue
SABINO, CAETANO P.; WAINWRIGHT, MARK; ANJOS, CAROLINA dos; SELLERA, FABIO P.; BAPTISTA, MAURICIO S.; LINCOPAN, NILTON; RIBEIRO, MARTHA S.
Inactivation kinetics and lethal dose analysis of antimicrobial blue light and photodynamic therapy.
Photodiagnosis and Photodynamic Therapy,
v. 28,
p. 186-191,
2019.
DOI:
10.1016/j.pdpdt.2019.08.022.
Disponível em: http://repositorio.ipen.br/handle/123456789/30437. Acesso em: $DATA.
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SELLERA, FABIO P.; GARGANO, RONALDO G.; ANJOS, CAROLINA dos; BAPTISTA, MAURICIO da S.; RIBEIRO, MARTHA S.
; POGLIANI, FABIO C..
Methylene blue-mediated antimicrobial photodynamic therapy: a novel strategy for digital dermatitis-associated sole ulcer in a cow – A case report.
Photodiagnosis and Photodynamic Therapy,
v. 24,
p. 121-122,
2018.
DOI:
10.1016/j.pdpdt.2018.09.004
Palavras-Chave:
methylene blue;
antimicrobial agents;
cattle;
inactivation;
photosensitivity;
therapy;
ulcers
SELLERA, FABIO P.; GARGANO, RONALDO G.; ANJOS, CAROLINA dos; BAPTISTA, MAURICIO da S.; RIBEIRO, MARTHA S.; POGLIANI, FABIO C.
Methylene blue-mediated antimicrobial photodynamic therapy: a novel strategy for digital dermatitis-associated sole ulcer in a cow – A case report.
Photodiagnosis and Photodynamic Therapy,
v. 24,
p. 121-122,
2018.
DOI:
10.1016/j.pdpdt.2018.09.004.
Disponível em: http://repositorio.ipen.br/handle/123456789/29344. Acesso em: $DATA.
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SELLERA, FABIO P.; BARBOSA, BRUNA S.; GARGANO, RONALDO G.; RISPOLI, VIVIAN F.P.; SABINO, CAETANO P.; OLLHOFF, RUDIGER D.; BAPTISTA, MAURICIO S.; RIBEIRO, MARTHA S.
; SA, LILIAN R.M. de; POGLIANI, FABIO C..
Methylene blue-mediated antimicrobial photodynamic therapy can be a novel non-antibiotic platform for bovine digital dermatitis.
Photodiagnosis and Photodynamic Therapy,
v. 34,
p. 1-8,
2021.
DOI:
10.1016/j.pdpdt.2021.102274
Abstract:
Background Bovine digital dermatitis (BDD) is one of the most important diseases that effect dairy cows. Methylene blue-mediated antimicrobial photodynamic therapy (MB-APDT) emerges as a promising technique to treat superficial infections in bovines. Methods Twenty BDD lesions located at the skin horn transition of the claw of pelvic limbs of 16 cows were treated by MB-APDT, using a red LED cluster (λ = 660 nm, irradiance =60 mW/cm2, exposure time = 40 s) combined with topical application of MB at 0.01 %; or by topical application of OXY (500 mg in 20 % solution). Each lesion was treated twice with an interval of 14 days. Lesions were weekly evaluated until day 28 by clinical analysis and by histological examination on days 0 and 28. Results Both treatments led to a similar reduction of lesions area. At day 28, three lesions treated by OXY did not present completely recovery, whereas no lesions were observed in MB-APDT group. OXY resulted in a slight increase in type I and III collagen levels, while MB-APDT led to a significant increase in the total area of both collagen types. An abundant number of spirochetes were histologically observed in all lesions before treatments. On the 28th day, five lesions treated by OXY still presented a slight number of spirochetes, whereas in MB-APDT group no spirochetes were evidenced. Conclusion Our findings suggest that MB-APDT is more effective than OXY and could be used in Veterinary practice to fight BDD.
Palavras-Chave:
methylene blue;
antimicrobial agents;
cattle;
inactivation;
photosensitivity;
therapy;
dermatitis;
infectious diseases
SELLERA, FABIO P.; BARBOSA, BRUNA S.; GARGANO, RONALDO G.; RISPOLI, VIVIAN F.P.; SABINO, CAETANO P.; OLLHOFF, RUDIGER D.; BAPTISTA, MAURICIO S.; RIBEIRO, MARTHA S.; SA, LILIAN R.M. de; POGLIANI, FABIO C.
Methylene blue-mediated antimicrobial photodynamic therapy can be a novel non-antibiotic platform for bovine digital dermatitis.
Photodiagnosis and Photodynamic Therapy,
v. 34,
p. 1-8,
2021.
DOI:
10.1016/j.pdpdt.2021.102274.
Disponível em: http://repositorio.ipen.br/handle/123456789/32053. Acesso em: $DATA.
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CABRAL, FERNANDA V.
; SELLERA, FABIO P.; RIBEIRO, MARTHA S.
.
Methylene blue-mediated antimicrobial photodynamic therapy for canine dermatophytosis caused by Microsporum canis: a successful case report with 6 months follow-up.
Photodiagnosis and Photodynamic Therapy,
v. 36,
p. 1-3,
2021.
DOI:
10.1016/j.pdpdt.2021.102602
Abstract:
Dermatophytosis is a superficial skin infection that widely effects companion animals. Miscrosporum canis is one of the most prevalent species isolated from dogs and cats, and because of the serious zoonotic potential, short-term treatment regimens are preferred to prevent the spread of disease either by direct contact or through contamination of the environment. Antimicrobial photodynamic therapy (APDT) has emerged as a promising strategy able to kill effectively a wide range of pathogens in a short period with minimal morbidity . In this case report, a 7-year-old male dog was diagnosed with dermatophytosis caused by M. canis. Methylene blue-mediated antimicrobial photodynamic therapy (MB-APDT) was applied over the lesions in two sessions with an interval of 7 days. The dog successfully healed, achieving a complete clinical cure after 21 days, without reports of recurrence after a follow-up period of 6 months. Therefore, MB-APDT could be a potential ally of small animal clinicians to treat superficial fungal diseases and should be further explored in Veterinary Medicine.
Palavras-Chave:
antimicrobial agents;
methylene blue;
veterinary medicine;
therapy;
skin diseases;
tinea
CABRAL, FERNANDA V.; SELLERA, FABIO P.; RIBEIRO, MARTHA S.
Methylene blue-mediated antimicrobial photodynamic therapy for canine dermatophytosis caused by Microsporum canis: a successful case report with 6 months follow-up.
Photodiagnosis and Photodynamic Therapy,
v. 36,
p. 1-3,
2021.
DOI:
10.1016/j.pdpdt.2021.102602.
Disponível em: http://repositorio.ipen.br/handle/123456789/32378. Acesso em: $DATA.
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NOVAES, G.A.; BLANK, M.H.; YOSHIMURA, T.M.
; RIBEIRO, M.S.
; PEREIRA, R.J.G..
Methylene blue-mediated antimicrobial photodynamic therapy on chicken semen.
Photodiagnosis and Photodynamic Therapy,
v. 41,
p. 1-12,
2023.
DOI:
10.1016/j.pdpdt.2023.103290
Abstract:
Background: Artificial insemination is widely employed in poultry, but high degrees of bacterial contamination are often observed in semen because of its passage through the cloaca. Consequently, most semen extenders for birds have antibiotics that could aggravate bacterial resistance. Methods: We evaluated the potential of antimicrobial photodynamic therapy (PDT) as an alternative to the use of antibiotics, and assessed whether changes in concentration and incubation time with methylene blue (MB), radiant exposure, and irradiance of light affect spermatozoa activity and bacteria in chicken semen. Results: Incubation with MB (< 25 µM) did not alter sperm motility, regardless of the pre-irradiation time (PIT, 1 or 5 min). Following 1 min of PIT with MB at 10 µM, samples were irradiated for 30, 60, 120, and 180 s at irradiances of 44, 29, and 17 mW/ cm² (660 nm LedBox). MB and light alone did not interfere with the analyzed parameters. However, when both factors were associated, increases in light dose led to greater reductions in sperm parameters, regardless of the irradiance used. Besides, PDT conditions that were less harmful to spermatozoa were not able to significantly reduce bacterial colonies in chicken semen. Conclusions: A failure in MB selectivity could explain unsuccessful bacterial reduction following PDT. Further research involving other photosensitizers or conjugating molecules to MB to target microbial cells is needed for PDT application in poultry breeders.
Palavras-Chave:
fowl;
antibiotics;
reproduction;
microorganisms;
chickens;
photodynamic therapy
NOVAES, G.A.; BLANK, M.H.; YOSHIMURA, T.M.; RIBEIRO, M.S.; PEREIRA, R.J.G.
Methylene blue-mediated antimicrobial photodynamic therapy on chicken semen.
Photodiagnosis and Photodynamic Therapy,
v. 41,
p. 1-12,
2023.
DOI:
10.1016/j.pdpdt.2023.103290.
Disponível em: http://repositorio.ipen.br/handle/123456789/33999. Acesso em: $DATA.
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BAPTISTA, ALESSANDRA
; NUNEZ, SILVIA C.
; SABINO, CAETANO P.
; MIYAKAWA, WALTER
; RIBEIRO, MARTHA S.
.
Morphological evaluation of Candida albicans after phododynamic therapy.
Photodiagnosis and Photodynamic Therapy,
v. 12,
p. 335,
2015.
Palavras-Chave:
morphological changes;
candida;
therapy;
antimicrobial agents;
scanning electron microscopy;
atomic force microscopy;
photosensitivity
BAPTISTA, ALESSANDRA; NUNEZ, SILVIA C.; SABINO, CAETANO P.; MIYAKAWA, WALTER; RIBEIRO, MARTHA S.
Morphological evaluation of Candida albicans after phododynamic therapy.
Photodiagnosis and Photodynamic Therapy,
v. 12,
p. 335,
2015.
Disponível em: http://repositorio.ipen.br/handle/123456789/25720. Acesso em: $DATA.
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ALVARENGA, LETICIA H.; GOMES, ANA C.; CARRIBEIRO, PAMELA; GODOY-MIRANDA, BIANCA; NOSCHESE, GIOVANE; RIBEIRO, MARTHA S.
; KATO, ILKA T.; BUSSADORI, SANDRA K.; PAVANI, CHRISTIANE; GERALDO, YHAGO G.E.; SILVA, DANIELA de F.T. da; HORLIANA, ANNA C.R.T.; WAINWRIGHT, MARK; PRATES, RENATO A..
Parameters for antimicrobial photodynamic therapy on periodontal pocket: randomized clinical trial.
Photodiagnosis and Photodynamic Therapy,
v. 27,
p. 132-136,
2019.
DOI:
10.1016/j.pdpdt.2019.05.035
Abstract:
Background: Antimicrobial photodynamic therapy (aPDT) has been investigated as an adjunctive to periodontal
treatment but the dosimetry parameters adopted have discrepancies and represent a challenge to measure efficacy.
There is a need to understand the clinical parameters required to obtain antimicrobial effects by using
aPDT in periodontal pockets. The aim of this study was to investigate parameters relating to the antimicrobial
effects of photodynamic therapy in periodontal pockets.
Material and methods: This randomized controlled clinical trial included 30 patients with chronic periodontitis.
Three incisors from each patient were selected and randomized for the experimental procedures. Microbiological
evaluations were performed to quantify microorganisms before and after treatments and spectroscopy was used
to identify methylene blue in the pocket. A laser source with emission of radiation at wavelength of ʎ=660 nm
and output radiant power of 100mW was used for 1, 3 and 5 min. One hundred μM methylene blue was used in
aqueous solution and on surfactant vehicle.
Results: The results demonstrated the absence of any antimicrobial effect with aqueous methylene blue-mediated
PDT. On the other hand, methylene blue in the surfactant vehicle produced microbial reduction in the group
irradiated for 5 min (p < 0.05). Spectroscopy showed that surfactant vehicle decreased the dimer peak signal at
610 nm.
Conclusion: Within the parameters used in this study, PDT mediated by methylene blue in a surfactant vehicle
reached significant microbial reduction levels with 5 min of irradiation. The clinical use of PDT may be limited
by factors that reduce the antimicrobial effect. Forms of irradiation and stability of the photosensitizers play an
important role in clinical aPDT.
Palavras-Chave:
methylene blue;
antimicrobial agents;
dosimetry;
clinical trials;
therapy;
dentistry;
bacteria;
irradiation;
absorption
ALVARENGA, LETICIA H.; GOMES, ANA C.; CARRIBEIRO, PAMELA; GODOY-MIRANDA, BIANCA; NOSCHESE, GIOVANE; RIBEIRO, MARTHA S.; KATO, ILKA T.; BUSSADORI, SANDRA K.; PAVANI, CHRISTIANE; GERALDO, YHAGO G.E.; SILVA, DANIELA de F.T. da; HORLIANA, ANNA C.R.T.; WAINWRIGHT, MARK; PRATES, RENATO A.
Parameters for antimicrobial photodynamic therapy on periodontal pocket: randomized clinical trial.
Photodiagnosis and Photodynamic Therapy,
v. 27,
p. 132-136,
2019.
DOI:
10.1016/j.pdpdt.2019.05.035.
Disponível em: http://repositorio.ipen.br/handle/123456789/30018. Acesso em: $DATA.
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GARCEZ, A.S.; NUNEZ, S.C.; FREGNANI, E.; SELLERA, D.P.; RIBEIRO, M.S.; SUZUKI, H..
Photodynamic antimicrobial therapy associated with surgical endodontic treatment.
Photodiagnosis and Photodynamic Therapy,
v. 8,
p. 181,
2011.
Palavras-Chave:
surgery;
antimicrobial agents;
dentistry;
therapy;
photosensitivity
GARCEZ, A.S.; NUNEZ, S.C.; FREGNANI, E.; SELLERA, D.P.; RIBEIRO, M.S.; SUZUKI, H.
Photodynamic antimicrobial therapy associated with surgical endodontic treatment.
Photodiagnosis and Photodynamic Therapy,
v. 8,
p. 181,
2011.
Disponível em: http://repositorio.ipen.br/handle/123456789/8885. Acesso em: $DATA.
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RIBEIRO, MARTHA S.
; MELO, LUCIANA S.A. de; FAROOQ, SAJID; BAPTISTA, ALESSANDRA
; KATO, ILKA T.; NUNEZ, SILVIA C.; ARAUJO, RENATO E. de.
Photodynamic inactivation assisted by localized surface plasmon resonance of silver nanoparticles: in vitro evaluation on Escherichia coli and Streptococcus mutans.
Photodiagnosis and Photodynamic Therapy,
v. 22,
p. 191-196,
2018.
DOI:
10.1016/j.pdpdt.2018.04.007
Abstract:
Localized surface plasmon resonance (LSPR) of gold nanoparticles has been reported to increase the antimicrobial effect of the photodynamic therapy. Although silver nanoparticles (AgNPs) are an efficient growth inhibitor of microorganisms, no studies exploring LSPR of AgNPs to enhance the photodynamic inactivation (PDI) have been related. In this work, we described the LSPR phenomenon of AgNP sand investigated its interaction with riboflavin, a natural photosensitizer. We evaluated the use of AgNPs coated with pectin (p-AgNP) in riboflavin (Rb)-mediated PDI of Escherichia colt (Gram- bacteria) and Streptococcus mutans (Gram + bacteria) using a blue light-emitting diode (lambda = 455 +/- 20 nm) of optical power 200 mW. Irradiance was 90 mW/cm(2) and radiant exposure varied according to the time exposure. Uptake of Rb and p-AgNP by the cells was evaluated by measuring the supernatant absorption spectra of the samples. We observed that LSPR of p-AgNPs was able to enhance the riboflavin photodynamic action on S. mutans but not on E. colt, probably due to the lower uptake of Rb by E. colt. Taken together, our results provide insights to explore the use of the LPRS promoted by silver nanostructures to optimize antimicrobial PDI protocols.
Palavras-Chave:
surfaces;
plasmons;
resonance;
photosensitivity;
inactivation;
nanoparticles;
silver;
antimicrobial agents;
light emitting diodes;
bacterial spores;
streptococcus;
escherichia coli
RIBEIRO, MARTHA S.; MELO, LUCIANA S.A. de; FAROOQ, SAJID; BAPTISTA, ALESSANDRA; KATO, ILKA T.; NUNEZ, SILVIA C.; ARAUJO, RENATO E. de.
Photodynamic inactivation assisted by localized surface plasmon resonance of silver nanoparticles: in vitro evaluation on Escherichia coli and Streptococcus mutans.
Photodiagnosis and Photodynamic Therapy,
v. 22,
p. 191-196,
2018.
DOI:
10.1016/j.pdpdt.2018.04.007.
Disponível em: http://repositorio.ipen.br/handle/123456789/28994. Acesso em: $DATA.
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SENNA, ANDRE M. de; VIEIRA, MARTHA M.F.
; MACHADO-DE-SENA, ROSA M.; BERTOLIN, APARECIDO O.; NUNEZ, SILVIA C.; RIBEIRO, MARTHA S.
.
Photodynamic inactivation of Candida ssp. on denture stomatitis. A clinical trial involving palatal mucosa and prosthesis disinfection.
Photodiagnosis and Photodynamic Therapy,
v. 22,
p. 212-216,
2018.
DOI:
10.1016/j.pdpdt.2018.04.008
Abstract:
Denture stomatitis (DS) is the most common oral fungal infection in denture wearers. Photodynamic inactivation (PDI) has been showing to be an effective technique in vivo against fungi, including fungal infections in the oral cavity. The disinfection of both oral mucosa and denture may represent a real advantage in terms of fungus control. This clinical study was designed to explore methylene blue (MB)-mediated PDI on oral mucosa and prosthesis of patients with DS. Subjects with DS were divided into two groups. One group received treatment based on the use of oral miconazole gel 2% (MIC). The other group received treatment by PDI using MB at 450 μg/mL and a diode laser (λ = 660 nm) with 100 mW and fluence of 28 J/cm2. Clinical outcome was evaluated regarding the degree of oral mucosa erythema and microbiological reduction of Candida spp. located in both palatal mucosa and prosthesis. Our results showed that PDI was significantly more effective than MIC in ameliorating inflammation after 15 days. Following 30 days, no statistically significant differences were observed between groups. Regarding the fungal burden, although the MIC group has presented more pronounced inactivation than PDI for both mucosa and prosthesis, no statistically significant differences were detected between them. This clinical study suggests that PDI can reduce fungal load and decrease the inflammation degree in patients with Candida-associated denture stomatitis.
Palavras-Chave:
photosensitivity;
oral cavity;
sterilization;
disinfectants;
inflammation;
mucous membranes;
methylene blue;
inactivation;
candida
SENNA, ANDRE M. de; VIEIRA, MARTHA M.F.; MACHADO-DE-SENA, ROSA M.; BERTOLIN, APARECIDO O.; NUNEZ, SILVIA C.; RIBEIRO, MARTHA S.
Photodynamic inactivation of Candida ssp. on denture stomatitis. A clinical trial involving palatal mucosa and prosthesis disinfection.
Photodiagnosis and Photodynamic Therapy,
v. 22,
p. 212-216,
2018.
DOI:
10.1016/j.pdpdt.2018.04.008.
Disponível em: http://repositorio.ipen.br/handle/123456789/29004. Acesso em: $DATA.
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SENA, R.M.M. de; CORREA, L.; KATO, I.T.
; PRATES, R.A.; SENNA, A.M.; SANTOS, C.C.; PICANCO, D.A.; RIBEIRO, M.S.
.
Photodynamic therapy has antifungal effect and reduces inflammatory signals in Candida albicans-induced murine vaginitis.
Photodiagnosis and Photodynamic Therapy,
v. 11,
n. 3,
p. 275-282,
2014.
Palavras-Chave:
female genitals;
candida;
therapy;
photosensitivity;
methylene blue;
laser radiation;
infrared radiation
SENA, R.M.M. de; CORREA, L.; KATO, I.T.; PRATES, R.A.; SENNA, A.M.; SANTOS, C.C.; PICANCO, D.A.; RIBEIRO, M.S.
Photodynamic therapy has antifungal effect and reduces inflammatory signals in Candida albicans-induced murine vaginitis.
Photodiagnosis and Photodynamic Therapy,
v. 11,
n. 3,
p. 275-282,
2014.
Disponível em: http://repositorio.ipen.br/handle/123456789/23669. Acesso em: $DATA.
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SABINO, C.P.
; NEVES, P.R.; LINCOPAN, N.; RIBEIRO, M.S.
.
Photodynamic therapy inhibits the antimicrobial effects of bacteriophage therapy in a invertebrate model of systemic infection.
Photodiagnosis and Photodynamic Therapy,
v. 12,
n. 3,
p. 357,
2015.
DOI:
10.1016/j.pdpdt.2015.07.127
Palavras-Chave:
photosensitivity;
antimicrobial agents;
invertebrates;
bacteriophages;
viruses;
pseudomonas;
in vitro;
therapy
SABINO, C.P.; NEVES, P.R.; LINCOPAN, N.; RIBEIRO, M.S.
Photodynamic therapy inhibits the antimicrobial effects of bacteriophage therapy in a invertebrate model of systemic infection.
Photodiagnosis and Photodynamic Therapy,
v. 12,
n. 3,
p. 357,
2015.
DOI:
10.1016/j.pdpdt.2015.07.127.
Disponível em: http://repositorio.ipen.br/handle/123456789/25721. Acesso em: $DATA.
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BARCESSAT, A.R.; HUANG, I.; RABELO, G.D.; ZEZELL, D.
; CORREA, L..
Proliferation index of keratinocytes in 4-NQO-induced oral dysplasias submitted to 5-aminolevulinic acid-mediated photodynamic therapy.
Photodiagnosis and Photodynamic Therapy,
v. 9,
Suppl. 1,
p. 59,
2012.
Palavras-Chave:
oral cavity;
mucous membranes;
cell proliferation;
keratin;
rats;
tongue;
photosensitivity;
therapy
BARCESSAT, A.R.; HUANG, I.; RABELO, G.D.; ZEZELL, D.; CORREA, L.
Proliferation index of keratinocytes in 4-NQO-induced oral dysplasias submitted to 5-aminolevulinic acid-mediated photodynamic therapy.
Photodiagnosis and Photodynamic Therapy,
v. 9,
p. 59,
2012.
Suppl. 1.
Disponível em: http://repositorio.ipen.br/handle/123456789/8899. Acesso em: $DATA.
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COURROL, LILIA C.; SILVA, FLAVIA R. de O.
; MASILAMANI, VALDIVEL.
SARS-CoV-2, hemoglobin and protoporphyrin IX: interactions and perspectives.
Photodiagnosis and Photodynamic Therapy,
v. 34,
p. 1-7,
2021.
DOI:
10.1016/j.pdpdt.2021.102324
Abstract:
Background: SARS-CoV-2 attacks hemoglobin through its structural protein ORF3a, dissociating the iron from the heme, as iron is necessary by cell machinery for virus replication. In this process protoporphyrin (PpIX) is released. Methods: The decrease in the hemoglobin levels observed in patients with Covid-19 is frequently accompanied by an increase in PpIX levels. This evidence was confirmed by the quantification of PpIX by high-performance liquid chromatography (HPLC). PpIX emission is observed in its two characteristic bands at approximately 635 nm and 705 nm. Results: This paper searches to understand the role of heme and PpIX inside the cells. Perspectives on the use of PpIX fluorescence as a sensor to monitor the presence of SARS-CoV-2 in the tissue, blood, urine, or feces to map the evolution and severity of the disease or to monitor the response of the Covid-19 treatment modalities were described. Conclusion: Fluorescence spectroscopy could be adopted as an excellent diagnostic technique for Covid-19, of low cost and high sensitivity. This method can potentially be used as a marker to monitor the response to the treatments. Photodynamic and sonodynamic therapies using the endogenous PpIX increased in the acute phase of the disease, could be employed for Covid-19 treatment.
Palavras-Chave:
coronaviruses;
hemoglobin;
protoporphyrins;
heme;
fluorescence
COURROL, LILIA C.; SILVA, FLAVIA R. de O.; MASILAMANI, VALDIVEL.
SARS-CoV-2, hemoglobin and protoporphyrin IX: interactions and perspectives.
Photodiagnosis and Photodynamic Therapy,
v. 34,
p. 1-7,
2021.
DOI:
10.1016/j.pdpdt.2021.102324.
Disponível em: http://repositorio.ipen.br/handle/123456789/32379. Acesso em: $DATA.
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COLLINA, GABRIELA A. da; CABRAL, FERNANDA V.
; MONTEIRO, CAROLINA M.; MACHADO, GABRIELA B.; GONCALVES, JOSE M.L.A.; FREIRE, FERNANDA; PRATES, RENATO A.; RIBEIRO, MARTHA S.
; PAVANI, CHRISTIANE.
The importance of combining methods to assess Candida albicans biofilms following photodynamic inactivation.
Photodiagnosis and Photodynamic Therapy,
v. 38,
p. 1-6,
2022.
DOI:
10.1016/j.pdpdt.2022.102769
Abstract:
Background: Methylene blue (MB)-mediated photodynamic inactivation (PDI) has shown good results in killing Candida spp. Although MB solutions are commonly used, new formulations have been designed to improve PDI. However, chemical substances in the formulation may interfere with the PDI outcome. In this sense, different methodologies should be used to evaluate PDI in vitro. Herein, we report different methodologies to evaluate the effects of PDI with an oral formulation (OF) containing 0.005% MB on Candida albicans biofilm. Methods: Biofilms were treated using the MB-OF, with 5 min pre-irradiation time and exposure to a 640 nm LED device (4.7 J/cm2). PDI was evaluated by the XTT reduction test, counting the colony forming units (CFU), a filamentation assay, crystal violet (CV) staining, and scanning electronic microscopy (SEM). Results: PDI was able to reduce around 1.5 log10 CFU/mL, even though no significant differences were noted in metabolic activity in comparison to the control immediately after PDI. A significant decrease in yeast to hyphae transition was observed after PDI, while the biofilm exhibited flattened cells and a reduced number of yeasts in SEM. The CV assay showed increased biomass. Conclusion: MB-OF-mediated PDI was effective in C. albicans biofilms, as it significantly reduced the CFU/mL and the virulence of surviving cells. The CV data were inconclusive, since the OF components interacted with the CV, making the data useless. Taken together, our data suggest that the association of different methods allows complementary responses to assess how PDI mediated by a formulation impacts biofilms.
Palavras-Chave:
antimicrobial agents;
methylene blue;
chemotherapy;
photodynamic therapy
COLLINA, GABRIELA A. da; CABRAL, FERNANDA V.; MONTEIRO, CAROLINA M.; MACHADO, GABRIELA B.; GONCALVES, JOSE M.L.A.; FREIRE, FERNANDA; PRATES, RENATO A.; RIBEIRO, MARTHA S.; PAVANI, CHRISTIANE.
The importance of combining methods to assess Candida albicans biofilms following photodynamic inactivation.
Photodiagnosis and Photodynamic Therapy,
v. 38,
p. 1-6,
2022.
DOI:
10.1016/j.pdpdt.2022.102769.
Disponível em: http://repositorio.ipen.br/handle/123456789/33201. Acesso em: $DATA.
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NARDINI, ELISA F.; ALMEIDA, THIAGO S.; YOSHIMURA, TANIA M.
; RIBEIRO, MARTHA S.
; CARDOSO, RIELSON J.; GARCEZ, AGUINALDO S..
The potential of commercially available phytotherapeutic compounds as new photosensitizers for dental antimicrobial PDT: a photochemical and photobiological in vitro study.
Photodiagnosis and Photodynamic Therapy,
v. 27,
p. 248-254,
2019.
DOI:
10.1016/j.pdpdt.2019.05.027
Abstract:
The present study evaluated the effectiveness of extracts of commercially available Curcuma longa, Citrus lemon,
Hamamelis virginiana and Hypericum perforatum as photosensitizers in Antimicrobial Photodynamic Therapy
(aPDT). Each photosensitizer (PS) was analyzed in a spectrophotometer between 350 and 750 nm to determine
the ideal light source. Once the absorption bands were determined, three light sources were selected. To determine
the concentration of use, the compounds were tested at different concentrations on bovine dentin
samples to evaluate the risk of staining. Once the concentration was determined, the PSs were evaluated for dark
toxicity and phototoxicity on fibroblast and bacteria culture. Each compound was then irradiated with each light
source and evaluated for the production of reactive oxygen species (ROS). The bacterial reduction was tested on
E. faecalis culture in planktonic form and on biofilm using an energy of 10 J and an Energy Density of 26 J/cm2.
The tested compounds exhibited light absorption in three bands of the visible spectrum: violet (405 nm), blue
(460 nm) and red (660 nm). At a 1:6 concentration, none of the compounds caused tooth staining as they did not
exhibit significant toxicity in the cells or bacterial suspension. Additionally, significant ROS production was
observed when the compounds were irradiated at each wavelength. When aPDT was performed on the plactonic
and biofilm bacteria, significant microbial reduction was observed in both cases, reaching a reduction of up to
5Logs. In conclusion, extracts of Curcuma longa, Citrus lemon, Hamamelis virginiana and Hypericum perforatum
exhibited potential for use as photosensitizing agents in aPDT.
Palavras-Chave:
plants;
biological materials;
medicinal plants;
photosensitivity;
photochemical reactions;
in vitro;
dentistry;
antimicrobial agents;
chemotherapy;
bacteria;
streptococcus;
therapy;
photochemistry;
teeth
NARDINI, ELISA F.; ALMEIDA, THIAGO S.; YOSHIMURA, TANIA M.; RIBEIRO, MARTHA S.; CARDOSO, RIELSON J.; GARCEZ, AGUINALDO S.
The potential of commercially available phytotherapeutic compounds as new photosensitizers for dental antimicrobial PDT: a photochemical and photobiological in vitro study.
Photodiagnosis and Photodynamic Therapy,
v. 27,
p. 248-254,
2019.
DOI:
10.1016/j.pdpdt.2019.05.027.
Disponível em: http://repositorio.ipen.br/handle/123456789/30486. Acesso em: $DATA.
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DIMMER, JESICA A.; CABRAL, FERNANDA V.; MONTOYA, SUSANA C.N.; RIBEIRO, MARTHA S.
.
Towards effective natural anthraquinones to mediate antimicrobial photodynamic therapy of cutaneous leishmaniasis.
Photodiagnosis and Photodynamic Therapy,
v. 42,
p. 1-6,
2023.
DOI:
10.1016/j.pdpdt.2023.103525
Abstract:
Background: Cutaneous leishmaniasis (CL) is an important tropical neglected disease with broad geographical
dispersion. The lack of effective drugs has raised an urgent need to improve CL treatment, and antimicrobial
photodynamic therapy (APDT) has been investigated as a new strategy to face it with positive outcomes. Natural
compounds have emerged as promising photosensitizers (PSs), but their use in vivo remains unexplored.
Purpose: In this work, we investigated the potential of three natural anthraquinones (AQs) on CL induced by
Leishmania amazonensis in BALB/c mice.
Study Design/Methods: :
Animals were infected and randomly divided into four groups: CG (control, non-treated group), G5ClSor-gL (treated
with 5-chlorosoranjidiol and green LED, 520±10 nm), GSor-bL and GBisor-bL (treated with soranjidiol and
bisoranjidiol, respectively, exposed to violet-blue LED, 410±10 nm). All AQs were assayed at 10 μM and LEDs
delivered a radiant exposure of 45 J/cm2 with an irradiance of 50 mW/cm2
. We assessed the parasite burden in
real time for three consecutive days. Lesion evolution and pain score were assessed over 3 weeks after a single
APDT session.
Results: G5ClSor-gL was able to sustain low levels of parasite burden over time. Besides, GSor-bL showed a
smaller lesion area than the control group, inhibiting the disease progression.
Conclusion: Taken together, our data demonstrate that monoAQs are promising compounds for pursuing the best
protocol for treating CL and helping to face this serious health problem. Studies involving host-pathogen
interaction as well as monoAQ-mediated PDT immune response are also encouraged.
Palavras-Chave:
skin diseases;
parasites;
protozoa;
light emitting diodes;
luminescence;
pain
DIMMER, JESICA A.; CABRAL, FERNANDA V.; MONTOYA, SUSANA C.N.; RIBEIRO, MARTHA S.
Towards effective natural anthraquinones to mediate antimicrobial photodynamic therapy of cutaneous leishmaniasis.
Photodiagnosis and Photodynamic Therapy,
v. 42,
p. 1-6,
2023.
DOI:
10.1016/j.pdpdt.2023.103525.
Disponível em: http://repositorio.ipen.br/handle/123456789/34086. Acesso em: $DATA.
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JESUS, VIVIANE P. dos S.; VIEIRA, PAULA F.A.; CINTRA, RICARDO C.; SANTANNA, LUCIANA B.; ZEZELL, DENISE M.
; CASTILHO, MAIARA L.; RANIERO LEANDRO.
Triple-negative breast cancer treatment in xenograft models by bifunctional nanoprobes combined to photodynamic therapy.
Photodiagnosis and Photodynamic Therapy,
v. 38,
p. 1-9,
2022.
DOI:
10.1016/j.pdpdt.2022.102796
Abstract:
Triple-negative breast cancer (TNBC) overexpresses the Epidermal Growth Factor Receptor (EGFR), a characteristic of different types of tumors, linked to worse disease prognosis and risk of recurrence. Conventional treatments are also aggressive and can be morbid.. Therefore, t improvement and development of new methods are notorious. Photodynamic Therapy (PDT) is an effective method for treating different types of cancer by using light radiation to activate a photosensitizing agent (drug) in molecular oxygen presence, promoting cell death., Improving drug uptake in target cells could contribute to PDT efficiency. Accordingly, we developed a bifunctional nanoprobe (BN), used in PDT as a a treatment method in vivo against breast cancer. The BN uses gold nanoparticles with active targeting through the Epidermal Growth Factor (EGF) protein and Chlorine e6 (Ce6) carriers. We evaluated the therapeutic efficacy of in vivo xenograft in 4 groups: Saline, BN, Ce6+PDT, and BN+PDT. As a result, we observed that the BN+PDT group exhibited an excellent effect with greater selectivity to tumor tissue and tissue damage when compared to the Saline, BN, and Ce6+PDT groups. The results indicate a potential impact on breast cancer treatment in vivo.. In conclusion, our data propose that the BN developed heightened PDT efficacy through cellular DNA repair effects and tumor microenvironment.
Palavras-Chave:
neoplasms;
mammary glands;
nanoparticles;
photodynamic therapy;
photosensitivity
JESUS, VIVIANE P. dos S.; VIEIRA, PAULA F.A.; CINTRA, RICARDO C.; SANTANNA, LUCIANA B.; ZEZELL, DENISE M.; CASTILHO, MAIARA L.; RANIERO LEANDRO.
Triple-negative breast cancer treatment in xenograft models by bifunctional nanoprobes combined to photodynamic therapy.
Photodiagnosis and Photodynamic Therapy,
v. 38,
p. 1-9,
2022.
DOI:
10.1016/j.pdpdt.2022.102796.
Disponível em: http://repositorio.ipen.br/handle/123456789/33202. Acesso em: $DATA.
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A elaboração do projeto do RI do IPEN foi iniciado em novembro de 2013, colocado em operação interna em julho de 2014 e disponibilizado na Internet em junho de 2015. Utiliza o software livre Dspace, desenvolvido pelo Massachusetts Institute of Technology (MIT). Para descrição dos metadados adota o padrão Dublin Core. É compatível com o Protocolo de Arquivos Abertos (OAI) permitindo interoperabilidade com repositórios de âmbito nacional e internacional.
O gerenciamento do Repositório está a cargo da Biblioteca do IPEN. Constam neste RI, até o presente momento 20.950 itens que tanto podem ser artigos de periódicos ou de eventos nacionais e internacionais, dissertações e teses, livros, capítulo de livros e relatórios técnicos. Para participar do RI-IPEN é necessário que pelo menos um dos autores tenha vínculo acadêmico ou funcional com o Instituto. Nesta primeira etapa de funcionamento do RI, a coleta das publicações é realizada periodicamente pela equipe da Biblioteca do IPEN, extraindo os dados das bases internacionais tais como a Web of Science, Scopus, INIS, SciElo além de verificar o Currículo Lattes. O RI-IPEN apresenta também um aspecto inovador no seu funcionamento. Por meio de metadados específicos ele está vinculado ao sistema de gerenciamento das atividades do Plano Diretor anual do IPEN (SIGEPI). Com o objetivo de fornecer dados numéricos para a elaboração dos indicadores da Produção Cientifica Institucional, disponibiliza uma tabela estatística registrando em tempo real a inserção de novos itens. Foi criado um metadado que contém um número único para cada integrante da comunidade científica do IPEN. Esse metadado se transformou em um filtro que ao ser acionado apresenta todos os trabalhos de um determinado autor independente das variáveis na forma de citação do seu nome.