Abstract: The aim of this study was to compare the rate of tooth displacement, quantity of root resorption, and alveolar bone changes in five groups: corticopuncture (CP), low-level laser therapy (LLLT), CP combined with LLLT (CP + LLLT), control (C), and negative control (NC). A total of 60 half-maxilla from 30 male Wistar rats (10 weeks old) were divided randomly into five groups: three (CP, LLLT, and CP + LLLT) test groups with different stimulation for accelerated-tooth-movement (ATM), one control (C) group, and one negative control (NC) group with no tooth movement. Nickel-titanium coil springs with 50 g of force were tied from the upper left and right first molars to micro-implants placed behind the maxillary incisors. For the CP and CP + LLLT groups, two perforations in the palate and one mesially to the molars were performed. For the LLLT and CP + LLLT groups, GaAlAs diode laser was applied every other day for 14 days (810 nm, 100 mW, 15 s). The tooth displacements were measured directly from the rat’s mouth and indirectly from microcomputer (micro-CT) tomographic images. Bone responses at the tension and compression sites and root resorption were analyzed from micro-CT images. The resulting alveolar bone responses were evaluated by measuring bone mineral density (BMD), bone volume fraction (BV/TV), and trabecular thickness (TbTh). Root resorption crater volumes were measured on both compression and tension sides of mesial and distal buccal roots. The tooth displacement in the CP + LLLT group was the greatest when measured clinically, followed by the CP, LLLT, and control groups (C and NC), respectively (p <0.05). The tooth movements measured from micro-CT images showed statistically higher displacement in the CP and CP + LLLT groups compared to the LLLT and control groups. The BMD, BV/TV, and TbTh values were lower at the compression side and higher at the tension side for all three test groups compared to the control group. The root resorption crater volume of the distal buccal root was higher in the control group, followed by CP, LLLT, and CP + LLLT, mostly at the compression site. Combining corticopuncture and low-level laser therapy (CP + LLLT) produced more tooth displacement and less root resorption at the compression side. The combined technique also promoted higher alveolar bone formation at the tension side.

Palavras-Chave: dentistry; teeth; laser radiation; low level counting; bone tissues; surgery; oral cavity

SUZUKI, SELLY S.; GARCEZ, AGUINALDO S.; REESE, PATRICIA O.; SUZUKI, HIDEO; RIBEIRO, MARTHA S.; MOON, WON. Effects of corticopuncture (CP) and low-level laser therapy (LLLT) on the rate of tooth movement and root resorption in rats using micro-CT evaluation. Lasers in Medical Science, v. 33, n. 4, p. 811-821, 2018. DOI: 10.1007/s10103-017-2421-5. Disponível em: http://repositorio.ipen.br/handle/123456789/28505. Acesso em: $DATA.

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Abstract: Photobiomodulation (PBM) has been studied to modify the cellular response to ionizing radiation. However, its combination with radiotherapy (RT) has not been reported in cancer treatment. The aim of this study was to evaluate the effects of PBM applied before RT on breast tumor-bearing mice. Female BALB/c mice were inoculated with breast 4T1 cells into mammary fat pad and divided into 4 groups (n =5 per group): control (with no treatment), only RT, and PBM combined to RT in two different protocols. RT was locally applied using a 60Co source with dose of 60 Gy in fractions of 15 Gy. For PBM, a red laser (660 nm, 500 mW/cm2) was used in two regimes: single exposure 24 h before RT (fluence of 150 J/cm2) and immediately before each RT session (fluence of 37.5 J/cm2 per session). After treatment, tumor volume, platelets, white and red blood cell levels were evaluated during 14 days. Our results showed no statistically significant differen ces in t umor volume, platelet and red blood cell levels comparing control, RT and PBM+RT groups. However, PBM was able to sustain normal white blood cell levels compared to RT and control groups. In addition, mice that received PBM concomitant with RT presented a longer survival. In fact, for this group only 12.5 % of the animals died during experimental period. These findings indicate that PBM could be combined to RT to provide therapeutic anti-cancer benefits.

Palavras-Chave: ionizing radiations; therapy; neoplasms; tumor cells; mice; mammary glands; radiotherapy

SILVA, C.R.; RIBEIRO, M.S. Effects of photobiomodulation on breast tumor- bearing mice before radiotherapy. Lasers in Medical Science, v. 35, n. 1, p. 269-269, 2020. DOI: 10.1007/s10103-019-02900-7. Disponível em: http://repositorio.ipen.br/handle/123456789/31111. Acesso em: $DATA.

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Abstract: The anti-inflammatory action of Photobiomodulation (PBM) is one of its most reproducible effects in vivo. New models to study PBMeffects and to investigate light dosimetry may bring different perspectives on the field. Based on the importance of establishing new experimental models and the advantages of using zebrafish for screening new drugs with potential anti-inflammatory effects the present investigation evaluated the effect of PBM on carragenan-induced abdominal edema in zebrafish. Thirty Danio rerio (± 1 g) zebrafish were randomly divided into five aquariums with 0.25L of water to establish three treatments: T1 - Naive non-treated; T2 – Saline 0.9%-injected; T3 - carrageenan-injected (CG) 3.5% in the coelomic cavity. T4 –injected (CG) 3.5% and treated with a diode laser λ=808nm, 50mWand 0.5J T5 –same parameters with 1J and T6 -1.5J. Abdominal volumes were measured by a caliper and the area was calculated 4 hours post-indu ction. Four hours after edema induction the results revealed an increase in the abdominal area of fish injected with CG 3.5% compared to negative control. The animals injected with CG and treated with PBM exhibited a reduction in abdominal edema in a dose-dependent manner. The 0.5J energy did not present a significant reduction compare do control (p=0.18), the 1J and 1.5J groups presented a significant difference (p=0.027 and p=0.005 respectively). The 1.5J group was not different from negative control (T2) p=0.37. The present study identified that zebrafish may represent a good model to investigate PBM effects and the response was dose dependent.

Palavras-Chave: inflammation; fishes; antipyretics; dosimetry; biological effects

NUNEZ, SILVIA C.; SILVA, IVES C. da; NASCIMENTO, JONATHAN S.; RIBEIRO, MARTHA S. Methodological study of photobiomodulation anti-edematous effects on carrageenan-induced inflammation on zebrafish. Lasers in Medical Science, v. 35, n. 1, p. 288-289, 2020. DOI: 10.1007/s10103-019-02900-7. Disponível em: http://repositorio.ipen.br/handle/123456789/31112. Acesso em: $DATA.

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Abstract: Mammal fat consists ofwhite and brown adipose tissues (WATand BAT). Although most fat is energy-storing WAT, brown adipocytes dissipate energy as heat. Obese subjects exhibit BAT hyporesponsiveness to classical stimuli as cold, hence increasing BAT thermogenic capacity could be an i n t er e s t i n g t a rget f o r t r eating obesity. Photobiomodulation (PBM) seems to be a feasible alternative to activate BAT, although it has not been applied for this purpose until now. In this study, adult C57Bl/6 mice were fed with a hyperlipidic diet (HF) during 15 weeks to induce obesity. Then, they were divided into two groups: HF and HF660. The interscapular BAT of HF660 mice was exposed to a single irradiation using a 662 ± 20 nm LED (5.7 Jcm-2 at 19 mWcm-2, delivered during 300 s). The HF group was sham-irradiated. Eutrophic animals were also used. Animals received 200 mCi of 18F-FDG and were exposed to cold for basal register of PET-CT images. After PBM or sh am treat ment, mice were once again exposed to cold before PET-CT image acquisition. Our results showed that PBM was able to activate BAT in obese mice through higher glucose uptake. In contrast, PBM and shamirradiated normal fed mice presented similar levels of glucose uptake. Taking together, our findings suggest that PBM applied to the BAT is able to promote glucose uptake by obese mice and could be an interesting approach to manage obesity and its correlated morbidities.

Palavras-Chave: adipose tissue; glucose; metabolic diseases; mice; irradiation

YOSHIMURA, TANIA; RIBEIRO, MARTHA S. Photobiomodulation activates glucose uptake in brown adipose tissue of obese mice. Lasers in Medical Science, v. 35, n. 1, p. 280-280, 2020. DOI: 10.1007/s10103-019-02900-7. Disponível em: http://repositorio.ipen.br/handle/123456789/31113. Acesso em: $DATA.

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Abstract: Low-level light therapy (LLLT) has been used in healthcare as a therapeutic strategy for diseases of various aetiologies. However, its effects on cancer is controversial. The aim of this work was to evaluate the effects of different light doses on blood cell count of breast cancer-bearing mice after a single application of LLLT. Tumour was induced by inoculation of 4T1 cells into the mammary fat pad of female BALB/c mice. Tumour volume was monitored with a digital calliper and when it reached 100 mm3, animals were irradiated by a red LED irradiator (660 ± 20 nm) at irradiance of 24.3 mW/cm2. The animals were randomly divided into groups (n= 5/group) that received energy densities of 1.8, 5.6 and 9.4 J/cm2. Control group was equally manipulated but did not receive irradiation. After treatment, data as tumour volume, platelets, white and red cell levels were registered weekly during 21 days. Our results showed that irradiated groups presented a longer survival compared to the control group, although no significant differences were observed among groups regarding tumour volume. Platelet levels of the irradiated groups remained closer to the reference values of healthy animals. Regarding the total number of white and red cells, no significant differences were observed between control and treated groups. These findings indicate that LLLT in a single application did not arrest tumour growth but may maintain platelet levels to increase survival of mice regardless the energy density protocol used.

Palavras-Chave: blood cells; light emitting diodes; neoplasms; dose rates; blood platelets

PEREIRA, S.T.; SILVA, C.R.; RIBEIRO, M.S. A single application of low-level light therapy in breast cancer-bearing mice: impact on blood cell count. Lasers in Medical Science, v. 35, n. 1, p. 274-274, 2020. DOI: 10.1007/s10103-019-02900-7. Disponível em: http://repositorio.ipen.br/handle/123456789/31109. Acesso em: $DATA.

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