Navegação por assunto "pupae"

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  • IPEN-DOC 23516

    ARTHUR, VALTER; MACHI, ANDRE R. ; ARTHUR, PAULA B.. Adult emergence and F1 generation egg and larval production after γ-irradiation of late pupae of Grapholita molesta (Lepidoptera: Tortricidae). Florida Entomologist, v. 99, special issue 2, p. 67-68, 2016.

    Abstract: The late pupal stage (pharate adult) of Grapholita molesta (Busck) (Lepidoptera: Tortricidae) close to adult emergence was irradiated with doses up to 350 Gy to determine a dose that could serve as a phytosanitary treatment against Lepidoptera that may pupate in shipped commodities. Adult emergence was not significantly reduced below that of the non-irradiated control until the dose reached 200 Gy. Not until the dose had been increased to 150 Gy was the number of eggs laid significantly less than the control, and oviposition was completely prevented at ≥ 300 Gy. Egg hatch was not significantly reduced compared with the non-irradiated control until the dose reached 150 Gy, and no eggs hatched at ≥ 250 Gy. This study supports a generic radiation dose for pupae of Lepidoptera of at least 250 Gy.

    Palavras-Chave: radiation doses; pupae; gamma radiation; agriculture; insects; pest control

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  • IPEN-DOC 27916

    NOGUEIRA, A.G. ; NEGRAO, B.G. ; ARTHUR, V.; VILLAVICENCIO, A.L.C.H. . Application of ionizing radiation in the "licuri" (Syagrus coronata (Mart.)) as phytosanitary treatment of the Pachymerus nucleorum beetle (Fabricius, 1792). Brazilian Journal of Radiation Sciences, v. 9, n. 1A, p. 1-9, 2021. DOI: 10.15392/bjrs.v9i1A.1576

    Abstract: Pachymerus nucleorum (Fabricius, 1792), is a pest of economic importance it causes direct damages to the “licuri” almonds. The objective of this study was to determine the lethal doses of gamma radiation for stages egg, larvae, and pupae of this insect as a phytosanitary treatment in “licuri”. The samples were irradiated in a Cobalt 60 irradiator, Gammacell-220 type, installed at the Institute of Energy and Nuclear Research - IPEN / CNEN at a dose rate of 748 Gy / h. Each treatment consisted of 3 replicates, so 180 licuri were irradiated in the doses of 50, 75, 100, and 125 Gy. After irradiation, the samples were stored at a temperature of 25 ± 5°C and relative humidity of 70 ± 5%. The evaluation of the fruits was performed by the emergence of insects. It is concluded that the dose of 125 Gy was sufficient to inhibit the development of insect life stages. Therefore, this dose of radiation can be used for control of the immature stages of the P. nucleorum.

    Palavras-Chave: coconuts; beetles; larvae; pupae; pest control; food processing; cobalt 60; lethal doses; radiation doses

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  • IPEN-DOC 26261

    NOGUEIRA, ALEXANDRA G. ; NEGRAO, BIANCA G ; ARTHUR, VALTER; VILLAVICENCIO, ANNA L.C.H. . Application of ionizing radiation in the coconuts of “Licuri” Syagrus Coronata (mart.) in the control of the Pachymerus Nucleorum beetle (Fabricius, 1792). In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 21-25, 2019, Santos, SP. Proceedings... Rio de Janeiro: Associação Brasileira de Energia Nuclear, 2019. p. 2141-2149.

    Abstract: The “licuri” fruit palm (Syagrus coronata) is harvested extractively from palm trees that grow in native forests, pastures, and in association with other cultures being part of the regional economy in the hinterland of Bahia. Among the problems related to the “licuri” palm is the insect specie Pachymerus nucleorum (Fabricius, 1792), which stands out as a pest of economic importance because it causes direct damages to the “licuri's” almonds. Due to this the objective of the work was to determine the lethal doses of gamma radiation for the phases of egg, larvae and pupae of Pachymerus nucleorum aiming at its control in almonds of coconuts of licuri. A Cobalt 60 irradiator, Gammacell-220 type, installed at the Institute of Energy and Nuclear Research - IPEN / CNEN, located in São Paulo / Brazil, at a dose rate of 748 Gy / h, was used to carry out the research with the Pachymerus nucleorum biological cycle. Each treatment for all stages of egg, larvae and pupae inside of coconuts consisted of 3 replicates with 12 coconuts each, in a total of 216 coconuts per test that were irradiated in the following doses: 0 (control), 50, 75, 100 and 125 Gy. After irradiation, the samples were stored at a temperature of 25 ± 5 ° C and relative humidity of 70 ± 5%, where the development of egg, larvae and pupae stages until adult transformation in irradiated almonds. From the results obtained it is concluded that the dose of 125 Gy was sufficient to interrupt the development of the phases of the insect. Therefore, this dose of radiation can be used for quarantine treatment of the immature stages of Pachymerus nucleorum in the coconuts of “licuri” Syagrus coronata palm.

    Palavras-Chave: beetles; cobalt 60; coconuts; dose rates; eggs; gamma radiation; insects; irradiation; larvae; pest control; pupae; radiation doses

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  • IPEN-DOC 17171

    ARTHUR, VALTER; SILVA, LUCIA C.A.S.; MODOLO, DEBORAH M.; LEANDRO, RODRIGO S.R.; ARTHUR, PAULA B.. Effects of gamma radiation on pupae of orintal fruit moth, Grapholita molesta (busck)(lepidoptera: tortricidae). In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; MEETING ON NUCLEAR APPLICATIONS, 10th; MEETING ON REACTOR PHYSICS AND THERMAL HYDRAULICS, 17th; MEETING ON NUCLEAR INDUSTRY, 2nd, October 24-28, 2011, Belo Horizonte, MG. Proceedings... Sao Paulo: ABEN, 2011, 2011.

    Palavras-Chave: insects; pupae; fruit flies; cobalt 60; moths; pest control; sterilization; radiation effects; gamma radiation

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  • IPEN-DOC 24110

    HADDAD, GIANNI Q.; MACHI, ANDRE R. ; ARTHUR, VALTER . Gamma radiation for all phases of life cycle of cotton bollworm Helicoverpa armigera aiming at its control. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 22-27, 2017, Belo Horizonte, MG. Proceedings... Rio de Janeiro, RJ: Associação Brasileira de Energia Nuclear, 2017.

    Abstract: Since the 1950s, scientists have used ionizing radiation to sterilize insects, which are released in nature to mate, but without any progeny. Known as the sterile insect technique (SIT), this insect control method traditionally uses ionizing radiations to sterilize insects, being a technique that does not generate residue, and can act in synergy with the other techniques within integrated pest management. For several years, Brazil has been fighting against the increase of pests, introducing new tactics and techniques within IPM programs, to overcome the resistance of chemical products, such as: reducing residues of pesticides. For some important crops of our country, we have a wide spectrum of pests occurring from beginning to end of the harvest, one of them is the cotton crop and among the key pests of this crop, we have some extremely important caterpillars among them Helicoverpa armigera. Due to this the objective this study was establishes doses of gamma radiation to sterilizing of the phases of : eggs, larvae, pupae and adults of H. armigera aiming their control. The experiment was carried out with application of gamma radiation from a Cobalt-60 source. The treatments consisted of doses of gamma radiation varying of according with the insect phase, being this variation of: 0 (control) to 400 Gy. The experiments with pupae and adult phases showed satisfactory results in the sterilization of H. armigera for use in autocide control programs. The sterilize dose to adult and pupae phase were 400 Gy and 100 Gy respectively, being the best doses for the application of the sterile insect technique to this pest in cotton.

    Palavras-Chave: bollworm; cobalt 60; cotton; dose rates; eggs; larvae; pest control; pupae; sterilization

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  • IPEN-DOC 05233

    FONTES, LUCIA da S. . Influencia da dieta irradiada sobre a longevidade e reproducao de Tribolium castaneum. 1997. Tese (Doutoramento) - Instituto de Pesquisas Energeticas e Nucleares - IPEN/CNEN-SP, Sao Paulo. 47 p. Orientador: Valter Arthur.

    Palavras-Chave: coleoptera; radurization; radappertization; larvae; gamma radiation; cobalt 60; insects; life cycle; radiodisinfestation; pupae; tribolium

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  • IPEN-DOC 23551

    ARTHUR, VALTER ; ARTHUR, PAULA B.; MACHI, ANDRE R. . Irradiation of Ecdytolopha aurantiana (Lepidoptera: Tortricidae) pupae in oxygen requires a lower dose to strongly reduce adult emergence and prevent reproduction than irradiation in air. Florida Entomologist, v. 99, special issue 2, p. 38-42, 2016.

    Abstract: The purpose of this research was to determine if γ-irradiation of lepidopteran pupae in oxygen would have a greater effect in either preventing adult emergence or in the successful development of F1 life stages than γ-irradiation of pupae in air. Pupae of the citrus fruit borer, Ecdytolopha aurantiana (Lima) (Lepidoptera: Tortricidae), were irradiated in either 100% oxygen or in normal air. In each experiment, thirty 10 d-old pupae of E. aurantiana in each of 4 replicates were treated with either 0, 100, 200 or 300 Gy of γ radiation. After irradiation the percent emergence of adults, and the percent viability of F1 eggs and larvae were compared with the corresponding results of irradiation in air. The results showed that irradiation of the pupae in pure oxygen significantly reduced the adult emergence and egg hatch compared to irradiation of the pupae in air. Thus irradiation in oxygen of pupae with 300 Gy completely prevented emerged adults from producing eggs. Therefore, irradiation of E. aurantiana pupae with 300 Gy in oxygen is a viable candidate as a phytosanitary treatment. In contrast, irradiation in air of pupae with 300 Gy resulted in 5% egg hatch, although 100% of these larvae died as first or second instars. Therefore based on the criterion of 100% mortality of F1 larvae, 300 Gy in air can also be considered to be a viable candidate for a phytosanitary treatment. These results indicate that the dose required for (PI) treatment may be reduced by increasing the oxygen gas content in fruit packages or containers.

    Palavras-Chave: irradiation; pupae; fruits; gamma radiation; air; gamma dosimetry; dosemeters

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  • IPEN-DOC 19273

    FRANCO, JOSE G.; FRANCO, CAIO H.; FRANCO, SUELY S.H.; ARTHUR, PAULA B.; ARTHUR, VALTER. Microwave radiation effects on the different stages of Sitophilus oryzae (Linné, 1763) (coleoptera, curculionidae) evolutive cycle in rice, focusing control. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; MEETING ON NUCLEAR APPLICATIONS, 11th; MEETING ON REACTOR PHYSICS AND THERMAL HYDRAULICS, 18th; MEETING ON NUCLEAR INDUSTRY, 3rd, November 24-29, 2013, Recife, PE. Proceedings... Sao Paulo: ABEN, 2013, 2013.

    Palavras-Chave: beetles; larvae; lethal doses; microwave radiation; pupae; radiation doses; radiation effects; radicidation; rice

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  • IPEN-DOC 23951

    ARTHUR, VALTER ; ARTHUR, PAULA B. ; MACHI, ANDRÉ R. . Pupation, adult emergence, and F1 egg hatch after irradiation of Spodoptera frugiperda (Lepidoptera: Noctuidae) last instars. Florida Entomologist, v. 99, Special issue 2, p. 59-61, 2016.

    Abstract: The fall armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), is a quarantine pest that may be found as eggs or larvae on a number of fresh vegetable and fruit commodities. The objective of the research was to evaluate the effects of gamma radiation as a phytosanitary treatment applied to the 5th (last) instar larvae. Larvae were reared on an artificial diet. Each treatment of twenty 15–20 d-old larvae had 5 repetitions. The larvae were irradiated with doses of gamma radiation of 0 (non-irradiated control), 50, 100, 200 and 300 Gy in a Cobalt-60 source (Gammacell-220) at a dose rate of 508 Gy/h. Pupation, adult emergence and hatching of F1 eggs were monitored. When the last instars were irradiated with100 Gy the rates of pupation, adult emergence and F1 egg hatch were 85, 39 and 50 percent, respectively, and the corresponding values in the non-irradiated control were 95, 85 and 90 percent, respectively. However, when the last instars were irradiated with 200 Gy, the pupation rate was reduced to 30 percent and adult emergence was reduced to 10 percent, and all of the adults that emerged were deformed, unable to fly, and died after about 2 h. Therefore, irradiation with 200 Gy is recommended as the appropriate dose for phytosanitary irradiation of S. frugiperda eggs and larvae. In addition this study supports the previously proposed generic dose of 250 Gy for eggs and larvae of lepidopteran pest species generally.

    Palavras-Chave: gamma radiation; pupae; insects; gamma radiation; larvae; cobalt 60

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  • IPEN-DOC 24149

    ARTHUR, VALTER ; FONTES, LUCIA da S.; ARTHUR, PAULA B. ; MACHI, ANDRE R. ; HARDER, MARCIA N.C.; ROSSI, RODRIGO S. ; FRANCO, JOSE G. ; FRANCO, SUELY S.H. . Quarantine treatment by gamma radiation for different development stages of Callosobruchus maculatus in bean Vigna sinensis. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 22-27, 2017, Belo Horizonte, MG. Proceedings... Rio de Janeiro, RJ: Associação Brasileira de Energia Nuclear, 2017.

    Abstract: The loss of stored grain caused by insects generates a problem of economic order of importance, due to concern about the increased supply of food for the world population is expanding. Associated with this fact, there is the problem of nutritional deficiency due to lack of protein, especially for the less privileged populations. The use of ionizing radiation in grains and products stored without a doubt can solve the problem of the losses in these products, since it does not induce resistance to insects and leaves no toxic residue in the products, and is considered an effective and safe method. The aim of the experiment was to determine the effect of ionizing radiation from cobalt-60 as a quarantine treatment for the different stages of development of Callosobruchus maculatus (Fabr., 1972) (Coleóptera, Chysomilidae) in bean Vigna sinensis. The experiment was conducted in the laboratory of Radiobiology and Environment CENA / USP., Piracicaba, SP, Brazil. Bean samples infested with eggs, larvae, pre-pupae and pupae C. maculatus, the experiment consisted of 4 replicates for each stage of the insect's life cycle, and each repetition consisted of 20 individuals (eggs, larvae, pre-pupae and pupae), a total of 200 subjects per treatment which were irradiated with doses of 0 (control), 25, 50, 75 and 100 Gy, a source of cobalt-60, Gammabeam-650 type, in a rate dose of 1.3 kGy / h. The experiment was conducted in a room with a relative of 25 ± 2 ° C temperature and humidity of 70 ± 5%. After 35 days of irradiation process were carried out evaluations of the number of insects emerged in each repetition within the treatments. From the results obtained it was concluded that the dose lethal to eggs and larvae was 25 Gy, while for pre-pupae was 50 Gy, to pupae 100 Gy was not sufficient to control the adult emergence.

    Palavras-Chave: beans; cobalt 60; dose rates; gamma radiation; insects; larvae; lethal doses; pupae; quarantine; radiation effects; radicidation

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  • IPEN-DOC 26260

    ARTHUR, PAULA B. ; VILLAVICENCIO, ANNA L.C.H. ; MACHI, ANDRE R. ; LEANDRO, RODRIGO S.R. ; ARTHUR, VALTER . Sterilizing of Alphitobius diaperinus (Panzer, 1797) (Coleoptera: Tenebrionidae) irradiated in pupa stage. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 21-25, 2019, Santos, SP. Proceedings... Rio de Janeiro: Associação Brasileira de Energia Nuclear, 2019. p. 2123-2128.

    Abstract: Worldwide the loss of stored grain is a problem of economic order of importance, in view of the concern of the increased supply of food for a world population increasingly expanding. Associated with this fact, there is the problem of nutritional deficiency due to lack of protein, especially for the less privileged populations in the resources of a country. This lack could be met by adequate supply of grain produced, requiring for it, a system that provides optimum grain storage conditions in securing the quality until the time of consumption. The use of radiation in stored grain can solve the problem of the losses in these products, as it does not induce resistance of insects and leaves no toxic residue to the consumer, and is considered an effective and safe method. The aim of the experiment was to determine the sterilizing dose of ionizing radiation from cobalt-60 to Alphitobius diaperinus (Panzer, 1797)(Coleoptera: Tenebrionidae) in peanuts irradiated in pupa stage. The experiment was conducted in the laboratory of Radiobiology and Environment of the Center for Nuclear Energy in Agriculture - CENA / USP., Piracicaba, SP, Brazil. Peanuts samples infested with pupae of A. diaperinus were utilized in the experiment that consisted of 8 treatments with 5 repetitions. Each repetition consisted of 20 pupae a total of 100 individuals per treatment. Were irradiated with doses of 0 (control), 25, 50, 75, 100, 125, 150 and 175 Gy, in a source of cobalt-60, Gammacell-220 type, with a rate dose of 381Gy / h. The experiment was conducted in a room with temperatures of 25 ± 5 ° C and relative humidity of 70 ± 5%. After of irradiation process was evaluated of the number of emerged adult insects in each repetition in the treatments. The results show that the sterilizing dose in F1 generation was 125 Gy and the pupa lethal dose 150 Gy. The dose of 150 Gy of gamma radiation can be used as phytosanitary treatment to control of immature stages of A. diaperinus infested peanuts.

    Palavras-Chave: beetles; cobalt 60; dose rates; insects; irradiation; peanuts; pupae; radiation dose units; radiation doses; sterilization

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  • IPEN-DOC 27889

    ARTHUR, P.B. ; VILLAVICENCIO, A.L.C.H. ; HARDER, M.N.C. ; MACHI, A.R. ; LEANDRO, R.S.R. ; ARTHUR, V. . Sterilizing of Alphitobius diaperinus (Panzer, 1797) (Coleoptera: Tenebrionidae) irradiated in pupa stage. Brazilian Journal of Radiation Sciences, v. 9, n. 1A, p. 1-7, 2021. DOI: 10.15392/bjrs.v9i1A.1445

    Abstract: The use of irradiation process in stored grain may be the solution for the losses caused in these products by insects, as it does not induce resistance in the insects and leaves no toxic residue, and is considered an efficient and safe method of control of insects. The aim of the experiment was to determine the sterilizing dose of ionizing radiation from cobalt-60 to Alphitobius diaperinus (Panzer, 1797) (Coleoptera: Tenebrionidae) in peanuts irradiated in pupa stage. The experiment was conducted in the laboratory of Radiobiology and Environment of the Center for Nuclear Energy in Agriculture - CENA / USP., Piracicaba, SP, Brazil. Peanuts samples infested with pupae of A. diaperinus were utilized in the experiment that consisted of 8 treatments with 5 repetitions. Each repetition consisted of 20 pupae a total of 100 individuals per treatment. Were irradiated with doses of 0 (control), 25, 50, 75, 100, 125, 150 and 175 Gy, in a source of cobalt-60, Gammacell-220 type, with a rate dose of 381Gy / h. The experiment was conducted in a controlled temperature of 25 ± 5 ° C and relative humidity of 70 ± 5%. After of irradiation process was evaluated of the number of emerged adult insects in each repetition in the treatments. The results showed that the sterilizing dose in F1 generation was 125 Gy and the pupa lethal dose 150 Gy. The dose of 150 Gy of gamma radiation can be used as phytosanitary treatment to control of immature stages of A. diaperinus infested peanuts.

    Palavras-Chave: cobalt 60; coleoptera; dose rates; gamma radiation; irradiation; lethal doses; peanuts; pupae; radiation dose units; radiation doses; sterilization

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A elaboração do projeto do RI do IPEN foi iniciado em novembro de 2013, colocado em operação interna em julho de 2014 e disponibilizado na Internet em junho de 2015. Utiliza o software livre Dspace, desenvolvido pelo Massachusetts Institute of Technology (MIT). Para descrição dos metadados adota o padrão Dublin Core. É compatível com o Protocolo de Arquivos Abertos (OAI) permitindo interoperabilidade com repositórios de âmbito nacional e internacional.

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O Repositório Digital do IPEN é um equipamento institucional de acesso aberto, criado com o objetivo de reunir, preservar, disponibilizar e conferir maior visibilidade à Produção Científica publicada pelo Instituto, desde sua criação em 1956.

Operando, inicialmente como uma base de dados referencial o Repositório foi disponibilizado na atual plataforma, em junho de 2015. No Repositório está disponível o acesso ao conteúdo digital de artigos de periódicos, eventos, nacionais e internacionais, livros, capítulos, dissertações, teses e relatórios técnicos.

A elaboração do projeto do RI do IPEN foi iniciado em novembro de 2013, colocado em operação interna em julho de 2014 e disponibilizado na Internet em junho de 2015. Utiliza o software livre Dspace, desenvolvido pelo Massachusetts Institute of Technology (MIT). Para descrição dos metadados adota o padrão Dublin Core. É compatível com o Protocolo de Arquivos Abertos (OAI) permitindo interoperabilidade com repositórios de âmbito nacional e internacional.

O gerenciamento do Repositório está a cargo da Biblioteca do IPEN. Constam neste RI, até o presente momento 20.950 itens que tanto podem ser artigos de periódicos ou de eventos nacionais e internacionais, dissertações e teses, livros, capítulo de livros e relatórios técnicos. Para participar do RI-IPEN é necessário que pelo menos um dos autores tenha vínculo acadêmico ou funcional com o Instituto. Nesta primeira etapa de funcionamento do RI, a coleta das publicações é realizada periodicamente pela equipe da Biblioteca do IPEN, extraindo os dados das bases internacionais tais como a Web of Science, Scopus, INIS, SciElo além de verificar o Currículo Lattes. O RI-IPEN apresenta também um aspecto inovador no seu funcionamento. Por meio de metadados específicos ele está vinculado ao sistema de gerenciamento das atividades do Plano Diretor anual do IPEN (SIGEPI). Com o objetivo de fornecer dados numéricos para a elaboração dos indicadores da Produção Cientifica Institucional, disponibiliza uma tabela estatística registrando em tempo real a inserção de novos itens. Foi criado um metadado que contém um número único para cada integrante da comunidade científica do IPEN. Esse metadado se transformou em um filtro que ao ser acionado apresenta todos os trabalhos de um determinado autor independente das variáveis na forma de citação do seu nome.