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Navegação IPEN por ano de publicação "2017"
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SARKIS, J.E.S.
; MARIN, R.C.
.
Uranium isotope ratio measurements by LA-HR-ICP-MS.
In: 60 YEARS OF IEA-R1: INTERNATIONAL WORKSHOP ON UTILIZATION OF RESEARCH REACTORS,
November 28 - December 01, 2017,
São Paulo, SP.
Abstract...
São Paulo, SP: Instituto de Pesquisas Energéticas e Nucleares,
2017.
p. 71-72.
Abstract:
This work describes the utilization of LA-HR-ICP-MS (laser ablation high resolution
inductively mass spectrometry) for characterize the uranium isotope ratio of a
UO2 pellet supplied and certified by New Brunswick Laboratory (NBL). This method
is useful measurements of 233U, 234U, 235U, 236U and 238U, reaching relative standard
deviations (RSD) from 1.55% to 2.45%. The Laser ablation ICP-MS technique
shows a high level of instability, compromising the precision of the measurements,
although could be made some adjustments to improve its stability. To reach the better
stability were made the adjustments of laser ablation and ICP-MS parameters,
such as RF power, laser beam diameter, defocusing of laser beam, auxiliary gas and
sample gas. The parameters which caused the greatest impact in order to improve
the stability signal were RF power, defocusing and laser beam diameter. To carry
out the adjustments of the parameters was used a glass standard NIST 610, supplied
and certified by National Institute of Standards and Technology (NIST). The
measurements were carried out on a single spot ablation with low energy density
and defocusing, which demonstrated to be the improved way to reach good signal stability. The results presented by the measurements revealed that the Laser ablation
ICP-MS technique offers a rapid and accurate way to perform uranium isotope
ratios without any sample preparation, since it allows carrying out the measurements
straight on the sample, besides to preserve the sample that is very important to
safeguards and nuclear forensics.
SARKIS, J.E.S.; MARIN, R.C.
Uranium isotope ratio measurements by LA-HR-ICP-MS.
In:
60 YEARS OF IEA-R1: INTERNATIONAL WORKSHOP ON UTILIZATION OF RESEARCH REACTORS,
November 28 - December 01, 2017,
São Paulo, SP.
Abstract...
São Paulo, SP: Instituto de Pesquisas Energéticas e Nucleares,
2017.
p. 71-72.
Disponível em: http://repositorio.ipen.br/handle/123456789/28772. Acesso em: $DATA.
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SILVA, EVELIN C. da
; BELLINI, MARIA H.
.
Upregulation of peroxideroxin-6 in human renal adenocarcinoma cells 786-0, after ionizing radiation.
In: ANAIS DA SOCIEDADE BRASILEIRA DE BIOCIENCIAS NUCLEARES,
09-11 de outubro, 2017,
São Paulo, SP.
Abstract...
Rio de Janeiro, RJ: Sociedade Brasileira de Biociências Nucleares,
2017.
p. 114-114.
Abstract:
Introduction: Renal cell carcinoma (RCC) accounts for 3% of human malignancies and
approximately 90% of renal malignancies and among urological tumors. RCC is quite resistant to
conventional radiotherapy. This technique allows the dose of radiation, in a single fraction, to be
precisely applied to the tumor and the tissues adjacent to it, most of the time, are spared. Proteomics
has allowed large-scale studies of protein expression in different tissues and body fluids, under
different conditions and / or times. Mass spectrometry allows the identification and quantification of
thousands of proteins and peptides in a biological fluid or lysed cells, and is analyzed on a platform
to identify differences in the expression of proteins associated with cancer cell proliferation and to
establish potential biomarkers predictive of the response therapy. The peroxideroxin- 6 (PRDX 6)
protein encoded by this gene is a member of the antioxidant protein family. The PRDX family
contains six members that function in detoxifying ROS and providing cytoprotection from internal
and It may play a role in the regulation of phospholipid turnover as well as in protection against
oxidative injury.
Aim: To analyze the expression of PRDX6 in 786-0 cells, after radiation.
Methods: A cell culture of the 786-0 cells was performed and to evaluate the mitotic potential, the
clonogenic assay was performed with doses of 2 to 10 Gy irradiated in GammaCell (CTR, IPEN)
and incubated for 10 days in normoxia conditions. After 10 days, the colonies of the respective
doses were stained with methanol 20% and crystal violet 0,5% and counted, and the multiple
comparisons was analized by One-way ANOVA followed by Bonferroni´s test and at the defined
dose the cells were irradiated and the cytoplasmic proteins were extracted by the PE kit Subcellular
proteome extraction (Merck, USA), dosed by the Lowry method and stored at -20º. For the
qualitative analysis of proteins, SDS-PAGE was performed with 50ug of protein and the protein
band obtained was digested and analyzed by nanoUPLC tandem nanoESI-MSE mass spectrometry
in the LNBio laboratory in Campinas-SP. The generated result was analyzed by MASCOT server
for peptides searchs and quantitatively analized by scaffoldTM 4.6 software.
Results: After the clonogenic assay was performed, 8 Gy was defined as the dose for cell
irradiation, an average protein yield of 786-0 non-irradiated 2,59± 0,07 mg/mL and 786-0 irradiated
with 3,13± 0,67 mg/mL was obtained. Mass spectrometry revealed the presence of the PRDX6
protein with a 95% coverage and a fold-change of 3.1 compared to the non-irradiated group.
Conclusion: The overexpression of PRDX6 after radiation, suggests a potential role for PRDX6 in
protection against oxidative stress and a radioresistance to renal cells,
SILVA, EVELIN C. da; BELLINI, MARIA H.
Upregulation of peroxideroxin-6 in human renal adenocarcinoma cells 786-0, after ionizing radiation.
In:
ANAIS DA SOCIEDADE BRASILEIRA DE BIOCIENCIAS NUCLEARES,
09-11 de outubro, 2017,
São Paulo, SP.
Abstract...
Rio de Janeiro, RJ: Sociedade Brasileira de Biociências Nucleares,
2017.
p. 114-114.
Disponível em: http://repositorio.ipen.br/handle/123456789/28820. Acesso em: $DATA.
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SILVA, F.R.O.
; LIMA, N.B.
; BRESSIANI, A.H.A.
; GOMES, L.
.
Upconversion luminescence of Yb3+: Tm3+ and Yb3+: Tm3+: Er3+- doped Beta-Tricalcium Phosphate nanoparticles.
In: INTERNATIONAL CONFERENCE ON LUMINESCENCE, 18th,
August 27 - September 01, 2017,
João Pessoa, PB.
Abstract...
2017.
p. 336-336.
Abstract:
A novel class of fluorescence nanoparticles of 5.5 mol% of Yb3+, 0.5 mol% of Er3+ and
0.5 mol% Tm3+: calcium deficient hydroxyapatite were synthesized by co-precipitation
method in aqueous solution (pH adjusted to 6) and specially treated with microwave radiation
at 1000°C for 10 minutes to produce nanocrystals of Yb:Tm: and Yb:Tm:Er:-tricalcium
phosphate (-TCP). As a result, we report for the first time, a single-phase -TCP:Yb:Tm:Er
and -TCP:Yb:Tm exhibiting an efficient visible and near infrared upconversion
luminescence from the 1G4 (blue emission), 4S3/2 (green emission), 3F2 (red emission) and 3H4
(near infrared emission) induced by the Yb3+ Tm3+ / Er3+ energy transfer under pulsed laser
excitation at 972 nm (Yb3+) with an average energy of 11 mJ. The emission decay curves of
the upconversion transients, from 1G4 excited state of Tm3+ and 4S3/2 excited level of Er3+,
indicate that ESA process occurs in -TCP:Yb/Tm/Er nanopowder measured for the 550 nm
luminescence of Er3+, which has a time constant (t2) of 0.4 s. However, Yb3+ Tm3+
upconversion (Up2) has a time constant (t2) of 14.4 s and does not exhibit ESA absorption. This -TCP activated by Yb3+ and Tm3+ ions constitutes a new nanobiomaterial that
can be used as diagnostic and therapeutic agents, affording deeper tissue penetration and
higher resolution and sensitivity for visible-near infrared bioimaging and treatments.
SILVA, F.R.O.; LIMA, N.B.; BRESSIANI, A.H.A.; GOMES, L.
Upconversion luminescence of Yb3+: Tm3+ and Yb3+: Tm3+: Er3+- doped Beta-Tricalcium Phosphate nanoparticles.
In:
INTERNATIONAL CONFERENCE ON LUMINESCENCE, 18th,
August 27 - September 01, 2017,
João Pessoa, PB.
Abstract...
2017.
p. 336-336.
Disponível em: http://repositorio.ipen.br/handle/123456789/28690. Acesso em: $DATA.
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BALDACONI, RICARDO H.
.
Unidade autônoma de monitoramento, sinalização e registro para o sistema de segurança do irradiador multipropósito de Cobalto-60
/ Autonomous monitoring unit, signs and registration for Cobalt-60 multipurpose irradiator safety system
.
2017.
Dissertação (Mestrado em Tecnologia Nuclear) -
Instituto de Pesquisas Energéticas e Nucleares - IPEN-CNEN/SP,
São Paulo.
107 p.
Orientador: Fábio Eduardo da Costa.
DOI:
10.11606/D.85.2018.tde-23022018-090648
Abstract:
O Irradiador Multipropósito de Cobalto-60 é uma unidade construída no Instituto de Pesquisas Energéticas e Nucleares, órgão da Comissão Nacional de Energia Nuclear. Esta instalação utiliza dos efeitos que a radiação gama produz ao interagir com um meio material e suas consequências. A radiação gama emitida pelo radioisótopo cobalto-60 é ionizante, com elevado poder de penetração, que ao interagir com os produtos, inclusive no interior de suas embalagens, transfere sua energia por meio de colisões aos elétrons dos átomos que constituem os produtos. Ao mesmo tempo que este processo de ionização é desejável em função de suas características deletérias aos microrganismos, a exposição indiscriminada às radiações ionizantes pelo ser humano ou animais apresentará danos e em doses elevadas poderão levar a morte. Diante destas circunstâncias, para garantir a segurança, os equipamentos utilizados para irradiação são construídos e operados sob normas rígidas de construção e operação. O sistema de segurança do Irradiador, constituído por um gerenciamento eletrônico de intertravamento de portas e exposição das fontes radioativas, é feito simultaneamente por um controlador lógico programável (CLP) e uma lógica de relés. Todas as informações, obtidas através das entrada e saídas do Irradiador, são monitoradas pelo sistema de segurança e enviadas para um computador com um programa supervisório. O propósito do trabalho foi a construção de uma Unidade Autônoma de Monitoramento, Sinalização e Registro para o Sistema de Segurança do Irradiador Multipropósito de Cobalto-60. O desenvolvimento deste equipamento permitiu o monitoramento e registro de eventos, até mesmo de tempos muito curtos, não detectáveis pelo CLP. Cada evento foi registrado em um cartão de memória, de forma a permitir que estes eventos possam ser posteriormente analisados em qualquer computador, mantendo todo e qualquer histórico de ocorrências. Este é um equipamento de monitoramento totalmente independente, não interferindo no funcionamento do sistema atual já homologado.
Palavras-Chave:
control equipment;
monitoring;
cobalt 60;
irradiation plants;
programming;
logic circuits;
engineered safety systems
BALDACONI, RICARDO H.
Unidade autônoma de monitoramento, sinalização e registro para o sistema de segurança do irradiador multipropósito de Cobalto-60.
Orientador: Fábio Eduardo da Costa.
2017.
107 f.
Dissertação (Mestrado em Tecnologia Nuclear) -
Instituto de Pesquisas Energéticas e Nucleares - IPEN-CNEN/SP,
São Paulo.
DOI:
10.11606/D.85.2018.tde-23022018-090648.
Disponível em: http://repositorio.ipen.br/handle/123456789/28822. Acesso em: $DATA.
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SANTANA, LUCIANA V. de; COTRIM, MARYCEL E.B.
; SAID, DAPHNE de S.; ULRICH, JOAO C.
; PIRES, MARIA A.F.
.
Uncertainty estimation for the determination of uranium by titrimetric method Davies & Gray.
In: CONGRESSO BRASILEIRO DE METROLOGIA, 9.; CONGRESSO INTERNACIONAL DE METROLOGIA MECANICA, 4.; CONGRESSO INTERNACIONAL DE METROLOGIA ELETRICA, 12.; CONGRESSO BRASILEIRO DE METROLOGIA DAS RADIAÇOES IONIZANTES, 4.; WORKSHOP DA REDE DE METROLOGIA QUIMICA DO INMETRO, 3.; CONGRESSO BRASILEIRO DE METROLOGIA OPTICA, 2.,
26-29 de novembro, 2017,
Fortaleza, CE.
Resumo...
Rio de Janeiro, RJ: Sociedade Brasileira de Metrologia,
2017.
p. 102-102.
SANTANA, LUCIANA V. de; COTRIM, MARYCEL E.B.; SAID, DAPHNE de S.; ULRICH, JOAO C.; PIRES, MARIA A.F.
Uncertainty estimation for the determination of uranium by titrimetric method Davies & Gray.
In:
CONGRESSO BRASILEIRO DE METROLOGIA, 9.; CONGRESSO INTERNACIONAL DE METROLOGIA MECANICA, 4.; CONGRESSO INTERNACIONAL DE METROLOGIA ELETRICA, 12.; CONGRESSO BRASILEIRO DE METROLOGIA DAS RADIAÇOES IONIZANTES, 4.; WORKSHOP DA REDE DE METROLOGIA QUIMICA DO INMETRO, 3.; CONGRESSO BRASILEIRO DE METROLOGIA OPTICA, 2.,
26-29 de novembro, 2017,
Fortaleza, CE.
Resumo...
Rio de Janeiro, RJ: Sociedade Brasileira de Metrologia,
2017.
p. 102-102.
Disponível em: http://repositorio.ipen.br/handle/123456789/28647. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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ANTONIO, P.L.
; GRONCHI, C.C.; PINTO, T.C.N.O.; CALDAS, L.V.E.
.
Ultraviolet radiation for the induction of PTOSL in CaSO4:Dy detectors.
In: INTERNATIONAL CONFERENCE ON LUMINESCENCE, 18th,
August 27 - September 01, 2017,
João Pessoa, PB.
Abstract...
2017.
p. 355-355.
Abstract:
The application of artificial sources of ultraviolet radiation (UVR) in various areas such as industry, medicine, dentistry and in research is increasing due to the technological advances of the last decades. This fact may cause health risks to workers if appropriate protection measurements were not adopted. The International Commission on Non-Radiation Protection (ICNIRP) recommends exposure limits to non-ionizing radiation. UVR measuring electronic instruments are expensive, and the use of dosimetric material is a good option for UV dosimetry. The phototransferred optically stimulated luminescence (PTOSL) was already shown to be a useful technique for UVR detection using Al2O3:C Landauer dosimeters. The PTOSL phenomenon involves the production of optically stimulated luminescence (OSL) by the phototransfer of charges to empty traps from deeper, filled traps. Thus, by filling the deeper traps of the detector with a pre-dose of ionizing radiation, and submitting the samples subsequently to an adequate thermal treatment to remove any trapped charge from the shallow (dosimetric) traps, charge can be transferred from the deep traps to the shallow traps by exposing the sample to UVR wavelengths. CaSO4:Dy+Teflon pellets produced at IPEN were used in this study. The PTOSL measurements were taken in an OSL reader system Risø, model TL/OSL-DA-20. The UV illumination procedures were performed in a system with a high-pressure mercury lamp. The CaSO4:Dy+Teflon samples were initially characterized in order to verify their reproducibility of OSL response and the variation of response in relation to the absorbed dose, using high doses of a 60Co source. The objective of this study is to characterize the material in terms of its luminescent properties. After this initial step, the samples were: pre-irradiated with a high dose; thermally treated; exposed to the UV source with different wavelengths; finally, the PTOSL responses were taken. The results were satisfactory and showed that the CaSO4:Dy samples present PTOSL response in function of the tested parameters, such as UV wavelength and irradiance.
ANTONIO, P.L.; GRONCHI, C.C.; PINTO, T.C.N.O.; CALDAS, L.V.E.
Ultraviolet radiation for the induction of PTOSL in CaSO4:Dy detectors.
In:
INTERNATIONAL CONFERENCE ON LUMINESCENCE, 18th,
August 27 - September 01, 2017,
João Pessoa, PB.
Abstract...
2017.
p. 355-355.
Disponível em: http://repositorio.ipen.br/handle/123456789/28693. Acesso em: $DATA.
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BAPTISTA, MAURICIO S.; CADET, JEAN; DI MASCIO, PAOLO; GHOGARE, ASHWINI A.; GREER, ALEXANDER; HAMBLIN, MICHAEL R.; LORENTE, CAROLINA; NUNEZ, SILVIA C.; RIBEIRO, MARTHA S.
; THOMAS, ANDRES H.; VIGNONI, MARIANA; YOSHIMURA, TANIA M.
.
Type I and type II photosensitized oxidation reactions: guidelines and mechanistic pathways.
Photochemistry and Photobiology,
v. 93,
n. 4,
p. 912-919,
2017.
DOI:
10.1111/php.12716
Abstract:
Here, 10 guidelines are presented for a standardized definition of type I and type II photosensitized oxidation reactions. Because of varied notions of reactions mediated by photosensitizers, a checklist of recommendations is provided for their definitions. Type I and type II photoreactions are oxygen-dependent and involve unstable species such as the initial formation of radical cation or neutral radicals from the substrates and/or singlet oxygen (O-1(2) (1)Delta(g)) by energy transfer to molecular oxygen. In addition, superoxide anion radical (O-2(.-)) can be generated by a charge-transfer reaction involving O-2 or more likely indirectly as the result of O-2(.)- mediated oxidation of the radical anion of type I photosensitizers. In subsequent reactions, O-2(.-) may add and/or reduce a few highly oxidizing radicals that arise from the deprotonation of the radical cations of key biological targets. O-2(.-) can also undergo dismutation into H2O2, the precursor of the highly reactive hydroxyl radical ((OH)-O-.) that may induce delayed oxidation reactions in cells. In the second part, several examples of type I and type II photosensitized oxidation reactions are provided to illustrate the complexity and the diversity of the degradation pathways of mostly relevant biomolecules upon one-electron oxidation and singlet oxygen reactions.
Palavras-Chave:
oxidation;
biological effects;
photosensitivity;
superoxide radicals;
anions
BAPTISTA, MAURICIO S.; CADET, JEAN; DI MASCIO, PAOLO; GHOGARE, ASHWINI A.; GREER, ALEXANDER; HAMBLIN, MICHAEL R.; LORENTE, CAROLINA; NUNEZ, SILVIA C.; RIBEIRO, MARTHA S.; THOMAS, ANDRES H.; VIGNONI, MARIANA; YOSHIMURA, TANIA M.
Type I and type II photosensitized oxidation reactions: guidelines and mechanistic pathways.
Photochemistry and Photobiology,
v. 93,
n. 4,
p. 912-919,
2017.
DOI:
10.1111/php.12716.
Disponível em: http://repositorio.ipen.br/handle/123456789/28510. Acesso em: $DATA.
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COSTA, I.F.; MOURA, J.L.; ADENES, R.E.; BRITO, H.F.; FELINTO, M.C.F.C.
; FAUSTINO, W.M.; CREMONA, M.; TEOTONIO, E.E.S..
Tuning colors in novel electroluminescent devices based on Al-indandionate complexes.
In: INTERNATIONAL CONFERENCE ON LUMINESCENCE, 18th,
August 27 - September 01, 2017,
João Pessoa, PB.
Abstract...
2017.
p. 262-262.
Abstract:
In the past years, the development of materials for organic light-emitting diodes (OLEDs) have attracted much attentions [1]. In this sense, the present work reports about syntheses, characterization, Photo (PL) - and electroluminescence (EL) properties of Al-indandionate complexes of formula [Al(aind)3], where aind: 2-acetyl-1,3-indandionate (acind), 2-benzoyl-1,3-indandionate (bind) or 2-(4-methyl)benzoyl-1,3-indandionate (mbind). These complexes were characterized by elemental analyses, Fourier-infrared absorption spectroscopy, thermogravimetric analyses and 1H-NMR. Photoluminescent spectra of the complexes in both powder and thin film exhibited overlapped broad bands associated to both fluorescence and phosphorescence (see time-resolved spectra, Fig. 1a) phenomena. The electroluminescent devices of configuration ITO/β-NPB/Al(aind)3/LiF/Al (1) exhibited emission in the red spectral region associated to electrophosphorescence (figure 1.b). On the other hand, the three layers devices of configuration ITO/β-NPB/spiro-2CBP/Al(aind)3/Al (2) showed an intensity band in green spectral region (figure 1.b). The OLEDs presented emission from low voltage (~8V), reaching the highest intensity around 16 V. According to these results, the Al-indandionate complexes act as both efficient electron transporters and emitting layers. In addition, these data suggest that TADF process is operative in the bilayer devices.
COSTA, I.F.; MOURA, J.L.; ADENES, R.E.; BRITO, H.F.; FELINTO, M.C.F.C.; FAUSTINO, W.M.; CREMONA, M.; TEOTONIO, E.E.S.
Tuning colors in novel electroluminescent devices based on Al-indandionate complexes.
In:
INTERNATIONAL CONFERENCE ON LUMINESCENCE, 18th,
August 27 - September 01, 2017,
João Pessoa, PB.
Abstract...
2017.
p. 262-262.
Disponível em: http://repositorio.ipen.br/handle/123456789/28686. Acesso em: $DATA.
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PENHALBEL, LUCIANO T.B.; PILLIS, MARINA F.
.
Tribological evaluation of intake valve and valve seat of a diesel cycle engine in real operating conditions.
In: SIMPOSIO INTERNACIONAL DE ENGENHARIA AUTOMOTIVA, 25.,
12-13 de setembro, 2017,
São Paulo, SP.
Proceedings...
São Paulo: Blucher,
2017.
DOI:
10.5151/engpro-simea2017-01
Abstract:
Automotive industry demands products that work under extreme conditions. The development of components for internal combustion engines is an action to improve this product, so that it reaches the required life with acceptable levels of wear and respecting the legal operating limits, such as values of emission pollutants. In view of these requirements, it is necessary to study components considered critical, which are known to influence the performance of the engine. So, within this universe the tribology of the intake valve and valve seat insert is an important set, that must receive attention to its wear and consequently its influence on the engine degradation, in the emission of pollutants and performance related to the operational maintenance requirements and life of the product. The aim of this work is to evaluate the behavior of the tribological intake pair, valve and valve seat insert, submitted to dynamometer and vehicular tests (real operating conditions), regarding the level and type of wear occurred in the pair. Correlating the results obtained with legal and operational performance requirements of a Diesel cycle engine, as well as the characterization of the wear type occurred in the tribological pair, with the related literature.
PENHALBEL, LUCIANO T.B.; PILLIS, MARINA F.
Tribological evaluation of intake valve and valve seat of a diesel cycle engine in real operating conditions.
In:
SIMPOSIO INTERNACIONAL DE ENGENHARIA AUTOMOTIVA, 25.,
12-13 de setembro, 2017,
São Paulo, SP.
Proceedings...
São Paulo: Blucher,
2017.
DOI:
10.5151/engpro-simea2017-01.
Disponível em: http://repositorio.ipen.br/handle/123456789/28908. Acesso em: $DATA.
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STEFFENS, DANIELA; MATHOR, MONICA B.
; SOSTER, PAULA R. da L.; VERGANI, GUSTAVO; LUCO, DAYANE P.; PRANKE, PATRICIA.
Treatment of a burn animal model with functionalized tridimensional electrospun biomaterials.
Journal of Biomaterials Applications,
v. 32,
n. 5,
p. 663-676,
2017.
DOI:
10.1177/0885328217735933
Abstract:
Laminin-functionalized poly-D,L-lactic acid scaffolds were produced. Following this, mesenchymal stem cells and keratinocytes
were seeded on biomaterials for the in vivo experiments, where the biomaterials with or without cells were
implanted. The analysis is comprised of the visual aspect and mean size of the lesion plus the histology and gene
expression. The results showed that the cells occupied all the structure of the scaffolds in all the groups. After nine
days of in vivo experiments, the defect size did not show statistical difference among the groups, although the groups
with the poly-D,L-lactic acid/Lam biomaterial had the lowest lesion size and presented the best visual aspect of the
wound. Gene expression analysis showed considerable increase of tumor growth factor beta 1 expression, increased
vascular endothelial growth factor and balance of the BAX/Bcl-2 ratio when compared to the lesion group. Histological
analysis showed well-formed tissue in the groups where the biomaterials and biomaterials plus cells were used. In some
animals, in which biomaterials and cells were used, the epidermis was formed throughout the length of the wound. In
conclusion, these biomaterials were found to be capable of providing support for the growth of cells and stimulated the
healing of the skin, which was improved by the use of cells.
Palavras-Chave:
burns;
biological materials;
stem cells;
keratin;
animal tissues;
skin;
skin damage;
electrodes
STEFFENS, DANIELA; MATHOR, MONICA B.; SOSTER, PAULA R. da L.; VERGANI, GUSTAVO; LUCO, DAYANE P.; PRANKE, PATRICIA.
Treatment of a burn animal model with functionalized tridimensional electrospun biomaterials.
Journal of Biomaterials Applications,
v. 32,
n. 5,
p. 663-676,
2017.
DOI:
10.1177/0885328217735933.
Disponível em: http://repositorio.ipen.br/handle/123456789/28535. Acesso em: $DATA.
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CONCEICAO, LEANDRO da
; LAY, SABINE; ROBAUT, FLORENCE; RENOU, GILLES; MUCCILLO, E.N.S.
; DJURADO, ELISABETH.
Transmission electron microscopy characterization of protective La0.7Sr0.3MnO3−delta coatings prepared by electrostatic spray deposition on ferritic alloy.
Functional Materials Letters,
v. 10,
n. 3,
p. 1750012-1 - 1750012-5,
2017.
DOI:
10.1142/S1793604717500126
Abstract:
The effect of La0:7Sr0:3MnO3 δ (LSM) coating on SS446 steel on air oxidation at 800○C was investigated by transmission electron
microscopy. Dense and crack free thin LSM films were prepared by electrostatic spray deposition. The microstructural characterization
was carried out on coated and uncoated interconnectors. A thin chromia scale at the alloy interface along with two spinel
phases were observed after long term oxidation in both cases. Specimens exhibit, in addition, an SiO2 layer at the interface with
steel due to the high content of Si in the steel. Significant changes in the thickness, morphology and composition occurred in the
reaction layer for the LSM coated steel. These effects are explained on the basis of changes in the diffusive fluxes during exposure
to the oxidation treatment. The implications of these effects for the degradation mechanism of LSM-based interconnects are
discussed.
Palavras-Chave:
transmission electron microscopy;
lanthanum oxides;
solid oxide fuel cells;
electrostatics;
deposition;
interconnected power systems;
perovskite
CONCEICAO, LEANDRO da; LAY, SABINE; ROBAUT, FLORENCE; RENOU, GILLES; MUCCILLO, E.N.S.; DJURADO, ELISABETH.
Transmission electron microscopy characterization of protective La0.7Sr0.3MnO3−delta coatings prepared by electrostatic spray deposition on ferritic alloy.
Functional Materials Letters,
v. 10,
n. 3,
p. 1750012-1 - 1750012-5,
2017.
DOI:
10.1142/S1793604717500126.
Disponível em: http://repositorio.ipen.br/handle/123456789/27709. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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SILVA, F.D.
; SEVILHANO, T.C.A.
; FREIRE, R.P.
; SUZUKI, M.F.
; OLIVEIRA, J.E.
; PERONI, C.N.
; RIBELA, M.T.C.P.
; BARTOLINI, P.
; SOARES, C.R.J.
.
Transient expression of recombinant human prolactin and thyrotropin in human embryonic kidney (Expi293FTM) suspension cells.
In: CONFERENCE ON PROTEIN EXPRESSION IN ANIMAL CELLS, 13th,
September 24-28, 2017,
Valencia, Spain.
Abstract...
2017.
Abstract:
Human prolactin (hPRL) and human thyrotropin (hTSH) are pituitary polypeptide hormones with key functions in the physiological regulation of the human body. hPRL is highly secreted during lactation, has important action in reproduction and for immunoregulation, among other functions. hTSH is related to the control of thyroid gland. The Chinese Hamster Ovary (CHO) and Human Embryonic Kidney (HEK293) cells are the most used hosts for expression of recombinant human proteins because they can be easily cultured in suspension conditions, and express high levels of proteins that have a relative similarity in post-translational modifications compared to their human counterparts. Our laboratory has experience in the synthesis of these proteins in the Escherichia coli periplasm (hPRL), adhered CHO (hPRL and hTSH), suspension CHO (hPRL) and adhered HEK293T cells (hTSH). The aim of this work was to produce hPRL and hTSH in suspension Expi293FTM cells for their characterization. The hPRL and hTSH cDNA were introduced into the commercial plasmid pcDNATM 3.4-TOPO® and 30 μg of these plasmids were used to transfect 30 mL of suspension Expi293FTM cells (2.5 x 106 cells/mL) in a 125 mL erlenmeyer, using 81 μL of ExpiFectamineTM transfection agent. After 16 h of transfection, 150 μL of Enhancer 1 and 1.5 mL of Enhancer 2 were added and the culture was maintained in an incubator at 37 °C, 8% CO2, at 125 rpm in orbital shaker. Samples of conditioned media (Expi293TM expression medium) were collected during 4 days and stored at -80 °C. These were analyzed by SDS-PAGE, ELISA, Western blotting, and HPLC. For the first time, hPRL and hTSH, were transiently expressed in human (Expi293FTM) suspension cells, the expression levels reaching, on the 3rd day, 46 μg of hPRL/mL and 116 μg of hTSH/mL. These results show that the expression is clearly dependent on the characteristics of the protein and that this methodology is very efficient to obtain high levels of human glycoproteins in a short time and will allow us to purify them and compare their glycosylation profiles of these to CHO-derived and human native pituitary hormones.
Palavras-Chave:
hormones;
embryos;
kidneys;
trh;
animal cells;
cho cells;
lth
SILVA, F.D.; SEVILHANO, T.C.A.; FREIRE, R.P.; SUZUKI, M.F.; OLIVEIRA, J.E.; PERONI, C.N.; RIBELA, M.T.C.P.; BARTOLINI, P.; SOARES, C.R.J.
Transient expression of recombinant human prolactin and thyrotropin in human embryonic kidney (Expi293FTM) suspension cells.
In:
CONFERENCE ON PROTEIN EXPRESSION IN ANIMAL CELLS, 13th,
September 24-28, 2017,
Valencia, Spain.
Abstract...
2017.
Disponível em: http://repositorio.ipen.br/handle/123456789/28435. Acesso em: $DATA.
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-
SILVA, FLAVIA R.O.
; NABESHIMA, CAMILA T.; COURROL, LILIA C.; BELLINI, MARIA H.
.
Transfer of aminolevulinic acid-induced protoporphyrin IX from tumor cells into the blood.
Open Access Journal of Cancer & Oncology,
v. 1,
n. 1,
p. 1-5,
2017.
Abstract:
This work evaluates the δ -aminolevulic acid (ALA)-induced Protoporphyrin IX (PpIX) synthesis and the PPIX transference from the tumor cells to the whole blood cells. Human prostate tumor cells (DU145 cells) received ALA at different periods and doses to analyze the PPIX synthesis. Thereafter, blood of healthy animals was added to the cultured tumorigenic cells to monitor the transference of the PPIX synthesized from them to the blood cells. Our results demonstrated that the intracellular production and accumulation of PPIX into prostate human tumor cells depends on the ALA doses and the time of exposition. Besides, PPIX molecules synthesized by tumor cells were transferred to the blood cells, but they were not transferred to the DMEM medium solution, showing that it is not a simple diffusion process of PPIX migration, but probably a complex efflux mechanism that cells have developed to transport it through the blood cells to regulate PPIX concentration inside them, since an excess of PPIX is highly toxic. These findings can be useful to understand the accumulation of PPIX to the blood of tumor-bearing animals and cancer patients, and it can be useful to design treatment and diagnosis protocols.
Palavras-Chave:
aminolevulinic acid;
protoporphyrins;
neoplasms;
therapy;
tumor cells;
blood cells;
fluorescence;
prostate;
diagnosis
SILVA, FLAVIA R.O.; NABESHIMA, CAMILA T.; COURROL, LILIA C.; BELLINI, MARIA H.
Transfer of aminolevulinic acid-induced protoporphyrin IX from tumor cells into the blood.
Open Access Journal of Cancer & Oncology,
v. 1,
n. 1,
p. 1-5,
2017.
Disponível em: http://repositorio.ipen.br/handle/123456789/27930. Acesso em: $DATA.
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LANGE, CAMILA N.
; FIGUEIREDO, ANA M.G.
; ENZWEILER, JACINTA; CASTRO, LILIANA
.
Trace elements status in the terrain of an impounded vehicle scrapyard.
Journal of Radioanalytical and Nuclear Chemistry,
2017.
DOI:
10.1007/s10967-016-5078-9
Abstract:
The impact of a scrapyard of impounded vehicles
in Sa˜o Paulo state on topsoil was evaluated for As, Ba,
Co, Cr, Cu, Mo, Ni, Pb, V, Zn and rare earth elements
(REEs). Mass fractions of all elements, except for Co, Cu,
Mo and Zn, were higher than reference values. Hot spots
were observed for most elements suggesting vehicular
source. The geoaccumulation index showed moderate
pollution of As. The enrichment factor pointed to a significant
enrichment of As, Mo and Pb. The normalization
of REEs to Earth’s crust values indicated a positive
anomaly of Ce. The results indicate a potential risk to the
soil quality of the scrapyard.
Palavras-Chave:
elements;
trace amounts;
soils;
neutron activation analysis;
vehicles;
urban areas;
contamination;
x-ray fluorescence analysis
LANGE, CAMILA N.; FIGUEIREDO, ANA M.G.; ENZWEILER, JACINTA; CASTRO, LILIANA.
Trace elements status in the terrain of an impounded vehicle scrapyard.
Journal of Radioanalytical and Nuclear Chemistry,
2017.
DOI:
10.1007/s10967-016-5078-9.
Disponível em: http://repositorio.ipen.br/handle/123456789/26998. Acesso em: $DATA.
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SARTORI, L.P.; LEONARDO, L.
; DAMATTO, S.R.
; SANTOS, N.D.
.
Trace elements determined in turtles trachemys scripta elegans from the urban area of São Paulo.
In: 60 YEARS OF IEA-R1: INTERNATIONAL WORKSHOP ON UTILIZATION OF RESEARCH REACTORS,
November 28 - December 01, 2017,
São Paulo, SP.
Abstract...
São Paulo, SP: Instituto de Pesquisas Energéticas e Nucleares,
2017.
p. 36-37.
Abstract:
The red-eared-turtle Trachemys scripta elegans, belonging to the suborder Cryptodira
and family Emydidae, is farmed in the Mississippi Valley in the USA and sold
throughout the world as one of the most traded pets. An increasing number of
species are frequently released by owners in natural wetlands and in urban areas.
This species is resistant and may spread parasites and threaten native turtle populations.
Many countries chose to perform euthanasia to control its population,
avoiding the threat to local biodiversity. Animals were studied here using Instrumental
Neutron Activation Analysis (INAA) to determine metallic elements Ca, Fe, Na,
Zn, aiming to assess the concentrations on different tissues in the body of ten female specimens. All of them donated already euthanized by DEPAVE (Parks and Green
Areas Department) U City of São Paulo. The bodies were open with electric saw,
with size between 18 and 23 cm of Carapace Length (CL), being all of them adults.
The samples analyzed were: superficial and bone carapaces liver, heart, lung, muscle
and kidneys. Approximately 200mg of the samples and reference materials, Soil 1
from IAEA and Montana II soil from NIST, were used. Those were irradiated at the
IEA-R1 nuclear research reactor at IPEN under a thermal neutron flux of 1012 n per
cm2 per s for 6h. Gamma spectrometry was used with a HPGe with relative efficiency
of 23% with an associated electronics, to perform the measurements. Methodology
validation regarding precision and accuracy were done using reference materials SL-
1 (Lake Sediment – IAEA) and Montana II soils (NIST). The Na, Ca, Zn and Fe
concentrations in shell bone and scute were in the same magnitude founded in the
literature. The majority of their growth occurs in early years of life then it would be
possible to infer that the concentrations found in the central part of their carapace
were related to previous years, and the concentrations that were found in the edges
can be related to the last months of animals life. Studies regarding cadmium intake
focuses in contamination based on animals diet, but as in this study there is few
information about the food source the animal received before living in city parks, and
before being euthanized they were put in a diet restricted to ration. The Zn concentrations
found in the carapace showed higher values in the scute when compared with
shell bone. Ca and Na presented the opposite behavior. Future studies related to
the metal concentrations in other parts of the animals could indicate the possibility
of using this specie as an environmental bioindicator.
SARTORI, L.P.; LEONARDO, L.; DAMATTO, S.R.; SANTOS, N.D.
Trace elements determined in turtles trachemys scripta elegans from the urban area of São Paulo.
In:
60 YEARS OF IEA-R1: INTERNATIONAL WORKSHOP ON UTILIZATION OF RESEARCH REACTORS,
November 28 - December 01, 2017,
São Paulo, SP.
Abstract...
São Paulo, SP: Instituto de Pesquisas Energéticas e Nucleares,
2017.
p. 36-37.
Disponível em: http://repositorio.ipen.br/handle/123456789/28733. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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-
DAMATTO, S.R.
; SANTOS, L.F.
.
Trace elements determined in sediment cores from Nhecolãndia Pantanal by INAA.
In: 60 YEARS OF IEA-R1: INTERNATIONAL WORKSHOP ON UTILIZATION OF RESEARCH REACTORS,
November 28 - December 01, 2017,
São Paulo, SP.
Abstract...
São Paulo, SP: Instituto de Pesquisas Energéticas e Nucleares,
2017.
p. 47-48.
Abstract:
Pantanal wetland is the world’s largest wetland, with an estimated area of about
200 000km2. This region is an alluvial plain and the Paraguay River and his tributaries
run through the wetland due to the low declivity of the plain in the north-south and
east-west direction; the water takes almost four months to cross the wetland, forming
different ecosystems, such as floodplains, lakes of fresh or saline water, rivers, and
others. In the last decades, this area has been affected by human activities, without
the suitable planning. The sediment of an aqueous system represents a great source
of study, as it is a deposit of solid material, formed by the wind, ice, or water on the
surface of the earth and the deposition of organic material from animals that live
there. From the sediment chemical analysis in combination with geochemical tools
is possible to estimate a probable source of the elements chemistry, whether natural
or anthropogenic. Trace elements from different sources tend to accumulate in the
sediment fine fraction (silt + clay), and are commonly mobile, what could generate
a transference of some metals to the environment. Therefore, the present work
analyzed the fine (silt + clay) and the coarse (medium sand + fine sand) fraction of
the sediment cores collected in four Salinas from Nhecolãndia Pantanal performing
the elementary chemical characterization of these sediment fractions and verify a
possible historical impact by anthropogenic sources and also to verify if some element
could accumulate in the sand fraction since the sediments from these ponds have
a high percentage of the coarse (sand) fraction; instrumental neutron activation
analysis technique (INAA) was used in the determination. Four sediment cores were collected in ponds located at Embrapa farm, 19 33023:31"S and 56 4057:56"W in
Nhecol andia Pantanal, Mato Grosso do Sul, using a PVC manual sediment sampler.
The cores were slice every 2cm and dried at 50 C; after drying, the samples were
ground and homogenized. The elements As, Ba, Ca, Ce, Co, Cr, Cs, Eu, Fe (%),
Hf, K (%), La, Lu, Na, Nd, Rb, Sb, Sc, Se, Sm, Ta, Tb, Th, U, Yb, and Zn were
determined (mg kg1) by Instrumental Neutron Activation Analysis (INAA) and the
analysis were performed in the coarse and in the fine fraction of the sediment. The
samples and reference material were irradiated for 8h at the IPEN Research Reactor
IEA-R1. The element As, Ba and Fe concentrations showed higher values than those
of the Upper Continental Crust, UCC, in the fine fraction of the sediment for the
studied cores. For the coarse fraction only the elements As, Ba and Hf showed
concentrations higher than the UCC values. The majority of the obtained results in
the INAA determination for the coarse and fine fraction suggested that there is no
expressive effect of the human activities in the region and this particular area until
the moment.
DAMATTO, S.R.; SANTOS, L.F.
Trace elements determined in sediment cores from Nhecolãndia Pantanal by INAA.
In:
60 YEARS OF IEA-R1: INTERNATIONAL WORKSHOP ON UTILIZATION OF RESEARCH REACTORS,
November 28 - December 01, 2017,
São Paulo, SP.
Abstract...
São Paulo, SP: Instituto de Pesquisas Energéticas e Nucleares,
2017.
p. 47-48.
Disponível em: http://repositorio.ipen.br/handle/123456789/28745. Acesso em: $DATA.
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-
WAKASUGI, D.S.M.
; SHIGASI, A.
; DAMATTO, S.R.
.
Trace elements determined in mineral waters using INAA from Parque das Águas de Lambari and Contendas.
In: 60 YEARS OF IEA-R1: INTERNATIONAL WORKSHOP ON UTILIZATION OF RESEARCH REACTORS,
November 28 - December 01, 2017,
São Paulo, SP.
Abstract...
São Paulo, SP: Instituto de Pesquisas Energéticas e Nucleares,
2017.
p. 25-26.
Abstract:
Mineral water, according to the Brazilian Law N 7.841 of August 8, 1945, "are
those from natural springs or springs artificially captured that have chemical composition
or physical or physicochemical properties other than ordinary waters, with
characteristics that give them a drug action". In this same law, mineral waters can
be classified according to their chemical composition in: oligomineral, radiferous,
alkaline-bicarbonated, alkalineearthy, sulfated, sulphurous, nitrated, chlorinated, ferruginous,
radioactive, thorioactive and carbogasous. In several Brazilian hydromineral
resorts, springs of radioactive mineral water are commercially exploited and are consumed
by the population that believes this practice is beneficial. For example, in
thermal parks like Caxambu, Cambuquira, São Lourenço and Lambari (MG), the waters
of the various springs are used for human consumption and are often associated
with medicinal use, like diuretic and cathartic waters (with properties of cleaning,
purification, facilitating hepato-biliary functions and stimulating intestinal function
directly or indirectly), and waters with antiphlogistic properties (anti-inflammatory).
Therefore, due to the recommendation of these waters as a form of treatment spent in spas, the present work was developed in Centro de Radiometria Ambiental – IPEN,
with the aid of those responsible for the spas of Lambari and Águas de Contendas
and, with the objective to perform an inorganic chemical characterization of the
mineral waters of Parque das Águas of Lambari and Águas de Contendas. Samples
of mineral waters were analyzed in duplicates and concentrated from 500 mL. The
samples were irradiated at the IPEN Research Reactor IEA-R1, for a period of 6h
under a thermal neutron flux of 1012 n cm2s1. In the samples of mineral waters of
the Parque das Águas of Águas de Contendas the elements Ca, Co, Fe, Hf, K, Na,
Rb, Sb, Sc, Th, Zn, La and Sm were found. Similarly, in the Parque das Águas of
Lambari the elements Ca, Co, Fe, K, Na, Rb, Sb, Sc, Zn, La and Sm were found.
The analysis of the samples of these water parks evidenced that the elements of
greatest occurrence were La and Co.
WAKASUGI, D.S.M.; SHIGASI, A.; DAMATTO, S.R.
Trace elements determined in mineral waters using INAA from Parque das Águas de Lambari and Contendas.
In:
60 YEARS OF IEA-R1: INTERNATIONAL WORKSHOP ON UTILIZATION OF RESEARCH REACTORS,
November 28 - December 01, 2017,
São Paulo, SP.
Abstract...
São Paulo, SP: Instituto de Pesquisas Energéticas e Nucleares,
2017.
p. 25-26.
Disponível em: http://repositorio.ipen.br/handle/123456789/28719. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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BRAGA, E.S.; AZEVEDO, J.S.; OLIVEIRA, A.L.; FAVARO, D.I.T.
.
Trace elements and nuclear abnormalities in fish species of two Brazilian estuarine regions – an attempt to increase the matrix for environmental monitoring.
Journal of Aquaculture & Marine Biology,
v. 6,
n. 2,
p. 1-11,
2017.
DOI:
10.15406/jamb.2017.06.00148
Abstract:
Trace elements As, Co, Fe, Hg, Se, Zn and Cr were determined in muscle tissue, gills and liver and nuclear abnormalities in erythrocytes (NAEs) were observed in 7 fish species (Chaetodipterusfaber, Paralanchurusbrasiliensis, Micropogoniasfurnieri, Achiruslineatus, Genyatremusluteus, Menticirrhus Americanos and Micropogoniasfurnieri), from two estuarine regions subject to different levels of anthropogenic impact (preserved and polluted) to provide information on contamination levels. The bioaccumulation in C. faber suggested that it should be proposed as a bioindicator of pollution in estuaries. The hydrological data: temperature, salinity and dissolved oxygen characterized the estuarine waters. A paradoxical result relating to As, Co, Fe and Zn concentrations was that, in general, these were higher in the fish species of the Cananeia estuary (a preserved area), while Hg concentrations and NAEs were higher in the species of the Santos-São Vicente estuary (SSV) (the polluted area). The bioaccumulation in tissue-organs decreased in the following order: liver>gill>muscle, with the exception of Hg that was liver>muscle with no detection in gills. Concerning the genotoxic effects, C. faber showed a more significant NAE, besides clastogenic effects, demonstrated by the greater frequency of micronuclei. Differences found between the fish species could be related to their feeding habits and to salinity. This is a contribution to making fishery efforts more profitable and enhancing the bioindicator bank.
Palavras-Chave:
trace amounts;
elements;
biological markers;
toxicity;
coastal regions;
ecosystems
BRAGA, E.S.; AZEVEDO, J.S.; OLIVEIRA, A.L.; FAVARO, D.I.T.
Trace elements and nuclear abnormalities in fish species of two Brazilian estuarine regions – an attempt to increase the matrix for environmental monitoring.
Journal of Aquaculture & Marine Biology,
v. 6,
n. 2,
p. 1-11,
2017.
DOI:
10.15406/jamb.2017.06.00148.
Disponível em: http://repositorio.ipen.br/handle/123456789/27731. Acesso em: $DATA.
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-
CUNICO, P.; FUNGARO, D.A.
; KUMAR, A..
Toxicity studies applied to evaluate the modified zeolites adsorbent for treating copper-complex dyes from simulated dyehouse wastewater.
In: SETAC LATIN AMERICA BIENNIAL MEETING, 12th,
September 7-10, 2017,
Santos, SP.
Abstract...
2017.
p. 35-36.
Abstract:
Textile dyes occur in wastewaters in different concentrations due to their poor fixation
to fabrics. Even at low concentrations, textile dyes can cause waste streams to become
highly colored. Aside from their negative aesthetic effects, certain textile dyes have
been shown to be toxic, and in some cases, these compounds are carcinogenic and
mutagenic. Zeolite synthesized from fly and bottom ashes and modified with
hexadecyltrimethylamonium (ZMF and ZMB, respectively) were used as adsorbent to
remove dyes - Solophenyl Navy and Solophenyl Turquoise and their hydrolyzed forms
Solophenyl Navy Hydrolyzed and Solophenyl Turquoise Hydrolyzed, respectively,
from simulated textile wastewater. The purpose of the research was to use bioassays
with Lemna minor, Ceriodaphnia dubia and Chironomus tepperi to determine the
acute and chronic toxicity of these dyes before and after adsorption process. The acute
results indicated C. tepperi showed to be less sensitive when compared with other test
organisms, showing values of 48hLC50 70.8 and 211 mgL-1 for SN and ST. On the
other hand, C. dubia showed to be very sensitive to the dyes tested (48hLC50 1.25; 54.5; 0.78 and 2.56 mgL-1 for SN, ST, SNH and STH), while L. minor presented
response for higher concentration of the dyes, as chlorosis, loss of roots, reduction of
the size and etc., showing values of EC50 of 18.9; 69.4; 10.9 and 70.9 mgL-1 for SN,
ST, SNH and STH). Chronic tests with C. tepperi showed changes in survival, growth,
development and gender differences for SN and ST dyes. After adsorption treatment
with ZMF the acute effects were substantially reduced for both dyes and their
hydrolyzed forms, showing absence of toxicity after treatment. However, the treatment
with ZMB showed no difference and/or increased toxicity in some cases. Toxicity
Identification Evaluation testes were realized and showed the baseline acute effects
were substantially reduced after treatment with Ethylenediaminetetraacetic acid,
showing the most of the toxicants in this study are cations metals. These experimental
results suggest both dyes, raw and hydrolyzed, are toxic and their removal are
necessary.
CUNICO, P.; FUNGARO, D.A.; KUMAR, A.
Toxicity studies applied to evaluate the modified zeolites adsorbent for treating copper-complex dyes from simulated dyehouse wastewater.
In:
SETAC LATIN AMERICA BIENNIAL MEETING, 12th,
September 7-10, 2017,
Santos, SP.
Abstract...
2017.
p. 35-36.
Disponível em: http://repositorio.ipen.br/handle/123456789/31259. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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-
BORRELY, S.
; FUNGARO, D.
.
Toxicity assays applications for assessing acute effects for radiation decomposition of organics in waters.
In: INTERNATIONAL CONFERENCE ON APPLICATIONS OF RADIATION SCIENCE AND TECHNOLOGY,
April 24-28, 2017,
Vienna, Austria.
Abstract...
Vienna: IAEA,
2017.
p. 108-108.
Abstract:
The knowledge for using electron bem irradiation for pollutants degradation is developing.
Textile effluents and pharmaceuticals were the samples submitted to irradiations and to
acute toxicity assays. An electron beam accelerator was the radiation source used for the
treatment in batch experiments. Dafnids, rotifers and bacteria were applied for toxicity
measurements. All the assays were performed at LEBA/IPEN (Environmental Biological
Assays Laboratory). Doses required for decomposition of organics in water and related
toxicity indicated that reduced colour of effluents with 2:5 kGy and 5 kGy. These doses
were also suitable for toxic effects removal at pharmaceutical solutions (fluoxetine in sewage;
propranolol and fluoxetine mixture and at fluoxetine and voltaren mixture). Part of real
textile effluent (about 35% of samples) were very toxic (CE50 5%) for daphnids and
luminescence Vibrio fischeri. The surfactants contained at textile effluent were the most toxic
compound. Vibrio fischeri luminescence was confirmed as one of the most sensitive assay,
followed by Ceriodaphnia dubia, Brachionus plicatis rotifers and Daphnia similis.
BORRELY, S.; FUNGARO, D.
Toxicity assays applications for assessing acute effects for radiation decomposition of organics in waters.
In:
INTERNATIONAL CONFERENCE ON APPLICATIONS OF RADIATION SCIENCE AND TECHNOLOGY,
April 24-28, 2017,
Vienna, Austria.
Abstract...
Vienna: IAEA,
2017.
p. 108-108.
Disponível em: http://repositorio.ipen.br/handle/123456789/27767. Acesso em: $DATA.
Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.
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tais como: Coleções, Ano de publicação, Títulos, Assuntos, Autores, Revista, Tipo de publicação são autoexplicativos. O filtro,
Autores IPEN
apresenta uma relação com os autores vinculados ao IPEN; o
ID Autor IPEN
diz respeito ao número único de identificação de cada autor constante no RD e sob o qual estão agrupados todos os seus trabalhos independente das variáveis do seu nome;
Tipo de acesso
diz respeito à acessibilidade do documento, isto é , sujeito as leis de direitos autorais, ID RT apresenta a relação dos relatórios técnicos, restritos para consulta das comunidades indicadas.
A opção
Busca avançada
utiliza os conectores da lógica boleana, é o melhor recurso para combinar chaves de busca e obter documentos relevantes à sua pesquisa, utilize os filtros apresentados na caixa de seleção para refinar o resultado de busca. Pode-se adicionar vários filtros a uma mesma busca.
Exemplo:
Buscar os artigos apresentados em um evento internacional de 2015, sobre loss of coolant, do autor Maprelian.
Autor: Maprelian
Título: loss of coolant
Tipo de publicação: Texto completo de evento
Ano de publicação: 2015
✔ Para indexação dos documentos é utilizado o Thesaurus do INIS, especializado na área nuclear e utilizado em todos os países membros da
International Atomic Energy Agency – IAEA
, por esse motivo, utilize os termos de busca de assunto em inglês; isto não exclui a busca livre por palavras, apenas o resultado pode não ser tão relevante ou pertinente.
✔ 95% do RD apresenta o texto completo do documento com livre acesso, para aqueles que apresentam o
significa que e o documento está sujeito as leis de direitos autorais, solicita-se nesses casos contatar a Biblioteca do IPEN,
bibl@ipen.br
.
✔ Ao efetuar a busca por um autor o RD apresentará uma relação de todos os trabalhos depositados no RD. No lado direito da tela são apresentados os coautores com o número de trabalhos produzidos em conjunto bem como os assuntos abordados e os respectivos anos de publicação agrupados.
✔ O RD disponibiliza um quadro estatístico de produtividade, onde é possível visualizar o número dos trabalhos agrupados por tipo de coleção, a medida que estão sendo depositados no RD.
✔ Na página inicial nas referências são sinalizados todos os autores IPEN, ao clicar nesse símbolo
será aberta uma nova página correspondente à aquele autor – trata-se da página do pesquisador.
✔ Na página do pesquisador, é possível verificar, as variações do nome, a relação de todos os trabalhos com texto completo bem como um quadro resumo numérico; há links para o Currículo Lattes e o Google Acadêmico ( quando esse for informado).
ATENÇÃO!
ESTE TEXTO "AJUDA" ESTÁ SUJEITO A ATUALIZAÇÕES CONSTANTES, A MEDIDA QUE NOVAS FUNCIONALIDADES E RECURSOS DE BUSCA FOREM SENDO DESENVOLVIDOS PELAS EQUIPES DA BIBLIOTECA E DA INFORMÁTICA.
O gerenciamento do Repositório está a cargo da Biblioteca do IPEN. Constam neste RI, até o presente momento 20.950 itens que tanto podem ser artigos de periódicos ou de eventos nacionais e internacionais, dissertações e teses, livros, capítulo de livros e relatórios técnicos. Para participar do RI-IPEN é necessário que pelo menos um dos autores tenha vínculo acadêmico ou funcional com o Instituto. Nesta primeira etapa de funcionamento do RI, a coleta das publicações é realizada periodicamente pela equipe da Biblioteca do IPEN, extraindo os dados das bases internacionais tais como a Web of Science, Scopus, INIS, SciElo além de verificar o Currículo Lattes. O RI-IPEN apresenta também um aspecto inovador no seu funcionamento. Por meio de metadados específicos ele está vinculado ao sistema de gerenciamento das atividades do Plano Diretor anual do IPEN (SIGEPI). Com o objetivo de fornecer dados numéricos para a elaboração dos indicadores da Produção Cientifica Institucional, disponibiliza uma tabela estatística registrando em tempo real a inserção de novos itens. Foi criado um metadado que contém um número único para cada integrante da comunidade científica do IPEN. Esse metadado se transformou em um filtro que ao ser acionado apresenta todos os trabalhos de um determinado autor independente das variáveis na forma de citação do seu nome.
A elaboração do projeto do RI do IPEN foi iniciado em novembro de 2013, colocado em operação interna em julho de 2014 e disponibilizado na Internet em junho de 2015. Utiliza o software livre Dspace, desenvolvido pelo Massachusetts Institute of Technology (MIT). Para descrição dos metadados adota o padrão Dublin Core. É compatível com o Protocolo de Arquivos Abertos (OAI) permitindo interoperabilidade com repositórios de âmbito nacional e internacional.
1. Portaria IPEN-CNEN/SP nº 387, que estabeleceu os princípios que nortearam a criação do RDI,
clique aqui.
2. A experiência do Instituto de Pesquisas Energéticas e Nucleares (IPEN-CNEN/SP) na criação de um Repositório Digital Institucional – RDI,
clique aqui.
O Repositório Digital do IPEN é um equipamento institucional de acesso aberto, criado com o objetivo de reunir, preservar, disponibilizar e conferir maior visibilidade à Produção Científica publicada pelo Instituto, desde sua criação em 1956.
Operando, inicialmente como uma base de dados referencial o Repositório foi disponibilizado na atual plataforma, em junho de 2015. No Repositório está disponível o acesso ao conteúdo digital de artigos de periódicos, eventos, nacionais e internacionais, livros, capítulos, dissertações, teses e relatórios técnicos.
A elaboração do projeto do RI do IPEN foi iniciado em novembro de 2013, colocado em operação interna em julho de 2014 e disponibilizado na Internet em junho de 2015. Utiliza o software livre Dspace, desenvolvido pelo Massachusetts Institute of Technology (MIT). Para descrição dos metadados adota o padrão Dublin Core. É compatível com o Protocolo de Arquivos Abertos (OAI) permitindo interoperabilidade com repositórios de âmbito nacional e internacional.
O gerenciamento do Repositório está a cargo da Biblioteca do IPEN. Constam neste RI, até o presente momento 20.950 itens que tanto podem ser artigos de periódicos ou de eventos nacionais e internacionais, dissertações e teses, livros, capítulo de livros e relatórios técnicos. Para participar do RI-IPEN é necessário que pelo menos um dos autores tenha vínculo acadêmico ou funcional com o Instituto. Nesta primeira etapa de funcionamento do RI, a coleta das publicações é realizada periodicamente pela equipe da Biblioteca do IPEN, extraindo os dados das bases internacionais tais como a Web of Science, Scopus, INIS, SciElo além de verificar o Currículo Lattes. O RI-IPEN apresenta também um aspecto inovador no seu funcionamento. Por meio de metadados específicos ele está vinculado ao sistema de gerenciamento das atividades do Plano Diretor anual do IPEN (SIGEPI). Com o objetivo de fornecer dados numéricos para a elaboração dos indicadores da Produção Cientifica Institucional, disponibiliza uma tabela estatística registrando em tempo real a inserção de novos itens. Foi criado um metadado que contém um número único para cada integrante da comunidade científica do IPEN. Esse metadado se transformou em um filtro que ao ser acionado apresenta todos os trabalhos de um determinado autor independente das variáveis na forma de citação do seu nome.