Navegação IPEN por Autores IPEN "MACHI, ANDRE R."

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  • IPEN-DOC 23516

    ARTHUR, VALTER; MACHI, ANDRE R. ; ARTHUR, PAULA B.. Adult emergence and F1 generation egg and larval production after γ-irradiation of late pupae of Grapholita molesta (Lepidoptera: Tortricidae). Florida Entomologist, v. 99, special issue 2, p. 67-68, 2016.

    Abstract: The late pupal stage (pharate adult) of Grapholita molesta (Busck) (Lepidoptera: Tortricidae) close to adult emergence was irradiated with doses up to 350 Gy to determine a dose that could serve as a phytosanitary treatment against Lepidoptera that may pupate in shipped commodities. Adult emergence was not significantly reduced below that of the non-irradiated control until the dose reached 200 Gy. Not until the dose had been increased to 150 Gy was the number of eggs laid significantly less than the control, and oviposition was completely prevented at ≥ 300 Gy. Egg hatch was not significantly reduced compared with the non-irradiated control until the dose reached 150 Gy, and no eggs hatched at ≥ 250 Gy. This study supports a generic radiation dose for pupae of Lepidoptera of at least 250 Gy.

    Palavras-Chave: radiation doses; pupae; gamma radiation; agriculture; insects; pest control

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  • IPEN-DOC 21074

    MACHI, ANDRE R.; ARTHUR, VALTER. Application of gamma radiation on longevity of some mites species (ACARI:Tetranychidade). In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE; MEETING ON NUCLEAR APPLICATIONS, 12th; MEETING ON REACTOR PHYSICS AND THERMAL HYDRAULICS, 19th; MEETING ON NUCLEAR INDUSTRY, 4th, October 4-9, 2015, São Paulo, SP. Proceedings... 2015.

    Palavras-Chave: mites; gamma radiation; cobalt 60; dose rates; mortality; radiation doses; radiation effects; survival time; tolerance

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  • IPEN-DOC 23517

    ARTHUR, VALTER ; MACHI, ANDRE R. . Development of phytosanitary irradiation against Aceria litchii (Trombidiformes: Eriophyidae) on lychee. Florida Entomologist, v. 99, special issue 2, p. 143-149, 2016.

    Abstract: The lychee erinose mite, Aceria litchii (Keifer) (Trombidiformes: Eriophyidae), is the most important pest of lychee (Litchi chinensis Sonn. (Sapindales: Sapindaceae) in parts of China, India, Southeast Asia, South Africa and Brazil. This study sought to develop the basis for phytosanitary irradiation of lychee to provide quarantine security against this pest. New methodology had to be devised for this purpose because the adult, the largest life stage—about 200 μ long—cannot be seen without magnification, and because this species does not survive more than a few d even on detached young lychee leaves, or under other artificial conditions. Initially we adapted a method devised by Azevedo et al. (2013) for keeping the adults alive long enough to evaluate the lethal effects of candidate acaricides for at 48 h post treatment. We collected infested leaves from a lychee orchard and irradiated then with doses increasing by increments of 200 Gy in the range 0–2,000 Gy. Each infested leaf had 30 to 40 adult mites. Each of 3 replicates involved ~816 adult mites and ~2,450 adult mites per treatment. Because of the presence of predators hidden within the erinea, we collected 30 adult mites per replicate immediately after irradiation, and placed them in a 14-cm-diam petri dish with a new young lychee leaf and moist cotton. We covered each petri dish with parafilm® to prevent escape of mites and loss humidity. At 24, 36, and 48 h post irradiation, we counted the numbers of live and dead mites. At 24 h post irradiation mortality occurred only in 1,800 and 2,000 Gy treatments, and it was only 1.7% in both treatments. At 36 h mortality had increased to 11.1 and 24.4% in the 1,600 and 2,000 Gy treatments, respectively. At 48 h statistically significant mortality occurred with all doses in the 200–2,000 Gy range; and it was 73.3% and 100.0% in the 1,800 and 2,000 Gy treatments, respectively. Since 2,000 Gy is unacceptable for phytosanitary irradiation, a dose had to be identified that prevents reproduction, i.e., a F1 generation. To prolong the survival of irradiated mites for at least 13 d, which appears to be the generation time of Aceria spp., we cut infested fragments of leaf blades, examined the under a microscope to remove adults and immature forms of predators mites, placed them in petri dishes, irradiated them with doses increasing by increments of 100 Gy in the range 0–500 Gy, and glued each irradiated leaf fragment onto a newly flushed leaf of a potted lychee tree in a screened greenhouse. Each treatment had 4 replications, and each consisted of ~10 adult mites for a total of ~40 adult mites per treatment in the first trial and 18 adults per replicate for a total of ~72 mites in the second trial. By 72 h post irradiation the mortality rate was considerably greater with all doses in the range 200–500 Gy than at 48 h in the earlier experiment, and the percent mortality with 100 Gy was significantly greater than in the control (0 Gy). Therefore, 72 h post irradiation seems to be the minimum time required for the lethal symptoms of irradiation to develop within the dose-range that is relevant to phytosanitary irradiation of fresh plant materials. In both the first and the second trials, moderate symptoms of erinose developed during 18 d post irradiation in the treatments with 0–300 Gy, but no erinose symptoms developed in the 400 and 500 Gy treatments. All symptomatic leaves displayed the patchy growth of erinea, i.e., abnormal felt-like hairs on the abaxial leaf epidermis. In addition by d 18 some galls had been formed on the upper sides of some of the leaves with erinea. These data show that irradiation of A. litchii with ≥ 400 Gy prevented it from reproducing, which is a critically important criterion of phytosanitary irradiation. Additional experiments are needed in the 300–400 Gy range to find the minimum dose required for phytosanitary irradiation.

    Palavras-Chave: agriculture; irradiation; mites; gamma radiation; pest control; fruits

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  • IPEN-DOC 26264

    ARTHUR, PAULA B. ; MACHI, ANDRE R. ; LEANDRO, R.S.R. ; ARTHUR, VALTER . Disinfestation of mangoes haden infested by Anastrepha fraterculus (Wied., 1830) (Diptera, Tephritidae), with gamma radiation. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 21-25, 2019, Santos, SP. Proceedings... Rio de Janeiro: Associação Brasileira de Energia Nuclear, 2019. p. 2184-2190.

    Abstract: The objective of experiment was determinate the radiation dose for disinfestation to mango Mangifera indica cv. Haden, infested by Anastrepha fraterculus larvae. For realization of the experiment, were collected fruits in the field, which were taking to Entomology laboratory where there was a infestation by the flies in cages during 72 hours period. Waited for the development of the larvae and before 5 – 8 days to infestation, the mangos were irradiated in a Cobalt-60 source with doses of: 0(control), 50, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1.100, 1.200 and 1.300 Gy. After the irradiation, the fruits were placed in climate chamber with 25 ±5°C of temperature and 70±5% of relative humidity, posteriorly waited the larvae exit to out of the fruit until the transformation in pupae and posteriorly in adult stage. By the results obtained our can concluded that the lethal dose to larvae in mangoes infested with 5-8 days after infestation were 600 Gy and 1.000 Gy (0.6 and 1 kGy) respectively. The dose of 50 Gy prevented the total adult emergence for both treatments.

    Palavras-Chave: cobalt 60; disinfestation; flies; gamma 10 devices; larvae; mangoes; pest control; radiation doses

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  • IPEN-DOC 26491

    MACHI, ANDRE R. . Efeitos da radiação ionizante nas fases do ciclo evolutivo de Aedes aegypti L. visando o seu controle através da técnica do inseto estéril / Ionizing radiation effects on stages of evolution cicle of Aedes aegypti L. aiming the control across of the insect sterile technique . 2019. Tese (Doutorado em Tecnologia Nuclear) - Instituto de Pesquisas Energéticas e Nucleares - IPEN-CNEN/SP, São Paulo. 75 p. Orientador: Valter Arthur. DOI: 10.11606/T.85.2019.tde-20052019-085251

    Abstract: Aedes aegypti L. é um dos principais insetos causadores de doenças no mundo, sendo assim, é importante trabalhos envolvendo o seu manejo. O objetivo do projeto foi avaliar os efeitos da radiação gama do Cobalto-60 nas fases do ciclo evolutivo de A. aegypti, visando a aplicação da TIE. O trabalho foi realizado no Laboratório de Radiobiologia e Ambiente do Centro de Energia Nuclear na Agricultura (CENA/USP) em Piracicaba- SP. A criação do A. aegypti foi mantida nas instalações do laboratório, sob condições de 25±2°C, UR 50-70% e fotofase de 12 horas. A irradiação de todas as fases do ciclo foi realizada em uma fonte de Cobalto-60 tipo Gammacell-220, instalada no CENA/USP, nas seguintes doses: 0 (controle) até 70 Gy sob uma taxa de dose de 0,356 kGy/hora. Quando ovos foram irradiados, avaliou-se viabilidade contando o número médio de larvas L4, pupas e adultos fêmeas e machos emergidos. Foi também avaliado a porcentagem de eclosão em larvas L1 da geração F1. Para larvas irradiadas, avaliaram-se a o número médio de larvas L4, pupas, adultos fêmeas e machos emergidos, porcentagem de eclosão em larvas L1 e larvas L4 F1. Das pupas irradiadas, avaliaram-se a média de eclosão de larvas F1, de larvas L4 F1, porcentagem de pupas F1 e média de adultos machos e fêmeas F1. Em adultos irradiados, avaliaram-se a média de eclosão de larvas F1, larvas L4 F1, pupas F1 e adultos machos e fêmeas F1. Avaliaram-se parâmetros de competitividade e longevidade de machos F1. Os resultados mostraram que as doses esterilizantes para as fases irradiadas: ovo, larva e pupa foram de 15 Gy, 20 Gy e 60 Gy, respectivamente. Já para adultos da geração F,1 as doses de 45 Gy e 50 Gy para macho e fêmea. Para a fase adulta, doses de 40 Gy e 45 Gy foram esterilizantes para machos e fêmeas. No teste de competitividade, a proporção de 10 machos estéreis: 1 macho não irradiado: 1 fêmea não irradiada causou 92,2% de ovos inviáveis, na dose de 50 Gy. A longevidade dos machos foi reduzida em pupas e adultos irradiados com a dose de 40 Gy.

    Palavras-Chave: mosquitoes; sterile insect release; sterile male technique; irradiation; sterilization; radiosterilization; biological effects; pest control; viral diseases; comparative evaluations

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  • IPEN-DOC 23528

    MACHI, ANDRE R. ; ARTHUR, VALTER ; SARRIES, GABRIEL A.; PIEDADE, SONIA M. de S.. Effect of gamma irradiation of gravid Tetranychus desertorum, T. urticae and Oligonychus ilicis (Trombidiformes: Tetranychidae) females on the viabilities and durations of F1 life stages. Florida Entomologist, v. 99, special issue 2, p. 186-190, 2016.

    Abstract: The objective of the study was to evaluate—as an alternative phytosanitary treatment—the effects of gamma irradiation of gravid spider mite females on the viabilities and durations of F1 eggs and deutonymphs. The study focused on Tetranychus urticae Koch, Tetranychus desertorum Banks and Oligonychus ilicis (McGregor) (Trombidiformes: Tetranychidae). Each mite species was identified under a stereomicroscope, and individual female mites of each species were each taken with a fine-tipped brush and transferred to a Petri dish—i.e., 1 female mite individualized per dish with 32 dishes per species. Each dish with a Tetranychus spp. female was provided with a jack bean (Canavalia ensiformis [L.] DC) (Fabales: Fabaceae) leaf, and each dish with an O. ilicis female was provided with a coffee (Coffea arabica L.; Gentianales: Rubiaceae) leaf. Gravid female mites were irradiated in a Gammacell 220 cobalt-60 source—dose rate 0.486 kGy/h—with doses of 0 (control), 100, 200, 300, and 400 Gy with 32 replications per mite species. Evaluations were made every 24 h for 22 d after irradiation by counting the numbers of eggs, deutonymphs, and adults and by recording the mortality in each stage. Statistical analyses were done with generalized linear models with either the quasi-binomial distribution or the Gaussian distribution—as appropriate—for analyses of mortality rates and durations (d) of the F1 eggs and deutonymphs of the 3 mite species. A 3 × 5 factorial experimental design was used in which factor A (3 levels) pertained to the species and factor B (5 levels) pertained to the 4 radiation doses plus the control. For Factor B polynomial regressions of dose rates on biological effects were used. The results showed progressive decreases in egg and deutonymph viabilities that were proportional with progressive increases in radiation doses. Against O. ilicis 200 Gy prevented all F1 neonates from developing beyond the first instar. Against T. urticae and T. desertorum 300 Gy prevented all F1 neonates from developing beyond the first instar. Irradiation of gravid females caused the durations of the F1 egg stage and the F1 deutonymph stage to be prolonged.

    Palavras-Chave: ionizing radiations; gamma radiation; mites; radiations; biological effects

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  • IPEN-DOC 25721

    MACHI, ANDRE R. ; FERRARI, LUIZ S. ; ARTHUR, VALTER . Efficacy of irradiated glyphosate against Brachiaria plantaginea L.. Journal of Agricultural Science, v. 10, n. 7, p. 383-389, 2018. DOI: 10.5539/jas.v10n7p383

    Abstract: Glyphosate irradiated with cobalt-60 type Gammacell 220 at different rates was sprayed on seedlings of Brachiaria plantaginea L., a common weed, grown in pots (one seedling with 3-4 leaves in each pot), at different concentrations equivalent to 1 L h-1 or 3 L h-1. Each sample containing 40 ml of the product (glyphosate) taken to a source of Cobalt-60 type Gammacell 220, under a dose rate of 0.312 kGy/hour in doses of: T0, control (water instead of glyphosate); T1, glyphosate at 1.0 L h-1; T2, glyphosate at 3.0 L h-1; T3, glyphosate irradiated at 250 Gy at 1.0 L h-1; T4, glyphosate irradiated at 500 Gy at 1.0 L h-1; T5, glyphosate irradiated at 750 Gy at 1.0 L h-1; and T6, glyphosate irradiated at 1000 Gy at 1.0 L h-1. The effects were assessed in terms of the extent of injury, where scale of 0% (no visible injury) to 100% (plant dead), number of tillers, weed survival and dry biomass produced by the plants. The most effective treatment was glyphosate irradiated at 250 Gy h-1 and applied at 1.0 L h-1. It is important to know that gamma radiation in glyphosate herbicide can be used without antagonistic interactions without cause resistance.

    Palavras-Chave: irradiation; herbicides; plants; gamma radiation; biomass; drying; cobalt 60; pest control; weeds

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  • IPEN-DOC 24110

    HADDAD, GIANNI Q.; MACHI, ANDRE R. ; ARTHUR, VALTER . Gamma radiation for all phases of life cycle of cotton bollworm Helicoverpa armigera aiming at its control. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 22-27, 2017, Belo Horizonte, MG. Proceedings... Rio de Janeiro, RJ: Associação Brasileira de Energia Nuclear, 2017.

    Abstract: Since the 1950s, scientists have used ionizing radiation to sterilize insects, which are released in nature to mate, but without any progeny. Known as the sterile insect technique (SIT), this insect control method traditionally uses ionizing radiations to sterilize insects, being a technique that does not generate residue, and can act in synergy with the other techniques within integrated pest management. For several years, Brazil has been fighting against the increase of pests, introducing new tactics and techniques within IPM programs, to overcome the resistance of chemical products, such as: reducing residues of pesticides. For some important crops of our country, we have a wide spectrum of pests occurring from beginning to end of the harvest, one of them is the cotton crop and among the key pests of this crop, we have some extremely important caterpillars among them Helicoverpa armigera. Due to this the objective this study was establishes doses of gamma radiation to sterilizing of the phases of : eggs, larvae, pupae and adults of H. armigera aiming their control. The experiment was carried out with application of gamma radiation from a Cobalt-60 source. The treatments consisted of doses of gamma radiation varying of according with the insect phase, being this variation of: 0 (control) to 400 Gy. The experiments with pupae and adult phases showed satisfactory results in the sterilization of H. armigera for use in autocide control programs. The sterilize dose to adult and pupae phase were 400 Gy and 100 Gy respectively, being the best doses for the application of the sterile insect technique to this pest in cotton.

    Palavras-Chave: bollworm; cobalt 60; cotton; dose rates; eggs; larvae; pest control; pupae; sterilization

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  • IPEN-DOC 25778

    MACHI, ANDRE R. ; MAYNE, RAQUEL R.; GAVA, MARCIO A.; ARTHUR, PAULA B. ; ARTHUR, VALTER . Gamma radiation sterilization dose of adult males in Asian tiger mosquito pupae. Insects, v. 10, n. 4, p. 1-11, 2019. DOI: 10.3390/insects10040101

    Abstract: The pathogen-carrying tiger mosquito, Aedes albopictus, has spread from theWestern Pacific and Southeast Asia to Europe, Africa, the Middle East, North and South America, and the Caribbean. This species of mosquito transmits arboviral infections, such as yellow fever, chikungunya, dengue, zika, and several encephalitides. The objective of this research was to provide a radiation dose inducing sterilization in adult male Ae. albopictus in the pupal stage. A cobalt-60 source of gamma radiation at a dose rate of 381 Gy/h was used. The pupae were irradiated with doses of 0 (control), 20, 30, 40, 50, and 60 Gy. Each treatment had a total of five replications using 60 pupae. After irradiation, the different phases of Ae. albopictus development (egg, larva, pupa, and adult) in the F1 generation were observed daily. Parameters such as viability, fertility, longevity, and mortality were recorded. The results from these studies showed that a dose of 60 Gy was necessary to sterilize 100% of the male Ae. albopictus pupae.

    Palavras-Chave: gamma radiation; sterilization; mosquitoes; viruses; ionizing radiations; radiation effects; cobalt 60; viral diseases; radiation doses

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  • IPEN-DOC 26924

    MACHI, ANDRE R. ; HARDER, MARCIA N.C.; ARTHUR, PAULA B. ; FRANCO, SUELY S.H.; ARTHUR, VALTER . Ionizing radiation and the influence of package to control of Sitophilus oryzae in rice. Australian Journal of Basic and Applied Sciences, v. 11, n. 15, p. 71-75, 2017. DOI: 10.22587/ajbas.2017.11.15.10

    Abstract: Objective: This study aimed to use the gamma radiation from Cobalt-60 to control of Sitophilus oryzae in package with previously infested rice and artificial reinfestation. Material and Methods: In the research, 3 types of sample were used: 2 commercial rice packages and 1 in crystal sugar packages. The samples were irradiated with a dose of 1.0kGy to promote disinfestations under a dose rate of 0.560 Gy/h. After 60 days were counted the number of live and dead insects in each package. Results: We concluded that the package containing holes for gases exchange from inside to outside of the package showed easily penetration of insects, while the two packages without holes prevented the reinfestation of insects. Conclusion: The dose of 1.0 kGy was sufficient to do the disinfestations of rice.

    Palavras-Chave: irradiation; ionizing radiations; packaging; cobalt 60; rice; pest control; insects; storage life; gamma radiation; dose rates; disinfestation

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  • IPEN-DOC 20717

    ARTHUR, VALTER; PIRES, JULIANA A.; SOUZA e SILVA, LUCIA C.A.; HARDER, MARCIA N.C.; FRANCO, SUELY S.H. ; FRANCO, JOSE G. ; MACHI, ANDRE R. ; ARTHUR, PAULA B.. Irradiação de cachaça comercial visando o envelhecimento precoce / Irradiation of commercial cachaça seeking precocious aging. Tecnologia e Ciencia Agropecuaria, v. 8, n. 3, p. 11-13, 2014.

    Palavras-Chave: beverages; sugar cane; alcohols; aging; irradiation procedures; cobalt 60

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  • IPEN-DOC 23551

    ARTHUR, VALTER ; ARTHUR, PAULA B.; MACHI, ANDRE R. . Irradiation of Ecdytolopha aurantiana (Lepidoptera: Tortricidae) pupae in oxygen requires a lower dose to strongly reduce adult emergence and prevent reproduction than irradiation in air. Florida Entomologist, v. 99, special issue 2, p. 38-42, 2016.

    Abstract: The purpose of this research was to determine if γ-irradiation of lepidopteran pupae in oxygen would have a greater effect in either preventing adult emergence or in the successful development of F1 life stages than γ-irradiation of pupae in air. Pupae of the citrus fruit borer, Ecdytolopha aurantiana (Lima) (Lepidoptera: Tortricidae), were irradiated in either 100% oxygen or in normal air. In each experiment, thirty 10 d-old pupae of E. aurantiana in each of 4 replicates were treated with either 0, 100, 200 or 300 Gy of γ radiation. After irradiation the percent emergence of adults, and the percent viability of F1 eggs and larvae were compared with the corresponding results of irradiation in air. The results showed that irradiation of the pupae in pure oxygen significantly reduced the adult emergence and egg hatch compared to irradiation of the pupae in air. Thus irradiation in oxygen of pupae with 300 Gy completely prevented emerged adults from producing eggs. Therefore, irradiation of E. aurantiana pupae with 300 Gy in oxygen is a viable candidate as a phytosanitary treatment. In contrast, irradiation in air of pupae with 300 Gy resulted in 5% egg hatch, although 100% of these larvae died as first or second instars. Therefore based on the criterion of 100% mortality of F1 larvae, 300 Gy in air can also be considered to be a viable candidate for a phytosanitary treatment. These results indicate that the dose required for (PI) treatment may be reduced by increasing the oxygen gas content in fruit packages or containers.

    Palavras-Chave: irradiation; pupae; fruits; gamma radiation; air; gamma dosimetry; dosemeters

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  • IPEN-DOC 23556

    ARTHUR, VALTER; NICASTRO, ROBERTO L.; SATO, MARIO E.; MACHI, ANDRE R. . Milbemectin and etoxazol acaricide resistant and susceptible strains of Tetranychus urticae (Trombidiformes: Tetranychidae) are equally radiosusceptible and unable to reproduce when irradiated with 400 Gy. Florida Entomologist, v. 99, special issue 2, p. 34-37, 2016.

    Abstract: The twospotted spider mite, Tetranychus urticae Koch (Trombidiformes: Tetranychidae), is considered one of the most important phytophagous mites causing considerable damage in several agricultural crops. The aim of this study was to evaluate the susceptibility to gamma irradiation of strains of T. urticae resistant (R) and susceptible (S) to the acaricides, milbemectin and etoxazol. The R and S strains for milbemectin and etoxazol were irradiated with 200 and 400 Gy of gamma radiation in a Gammacell 220 source to evaluate the effects of gamma radiation on the growth rate of the mites. A dose of 400 Gy totally eliminated populations of both R and S strains of T. urticae within 10 d. A dose of 200 Gy was not sufficient to totally eliminate both T. urticae strains, but it significantly reduced egg viability of both strains. In the most likely measure of efficacy for phytosanitary irradiation of mites—i.e., prevention of F1 egg hatch when parent adults are irradiated—no differences were found in response to irradiation among the 4 strains.

    Palavras-Chave: irradiation; mites; pest control; arachnids; agriculture; cobalt 60; gamma radiation

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  • IPEN-DOC 23543

    MACHI, ANDRE R. ; ARTHUR, VALTER . Oxygen atmosphere potentiates radiation effects on Brevipalpus yothersi (Trombidiformes: Tenuipalpidae). Florida Entomologist, v. 99, special issue 2, p. 18-23, 2016.

    Abstract: The objective of the study was to compare the effect of pure oxygen to that of ambient air on gamma irradiation of Brevipalpus yothersi (Baker) (Trombidiformes: Tenuipalpidae). Flasks containing the mites were irradiated in a Gammacell-220 irradiator with Cobalt-60 emitting gamma radiation at a rate of 381 Gy/h. Seventy mites per flask replicated 4 times were irradiated in either pure oxygen or air with 0 (control), 200, 230, 270, or 300 Gy as the intended doses. All eggs, deutonymphs and adults were counted each day and the parameters of egg production, egg hatch, development and mortality were recorded. Data were analyzed with ANOVA and means were separated with Tukey’s Honestly Significant Difference (HSD) test at 5% probability. Generally, irradiation of females with progressively larger doses—whether in oxygen or in air—resulted in progressively greater negative biological effects, and these effects were greater when females were irradiated in oxygen than in air. Non-irradiated gravid females exposed to pure oxygen deposited 79.3 ± 0.3 eggs per female compared to 73.0 ± 0.3 per female in ambient air. The numbers of eggs oviposited by females irradiated with the largest dose (300 Gy) were 29.1 ± 0.2 in air and 18.1 ± 0.3 in oxygen. In the ambient air + 270 Gy treatment egg hatch was 3.8 ± 0.1%, but in the oxygen + 270 Gy treatment it was 0%. When females were irradiated in air with 300 Gy, egg hatch was totally prevented. The number of F1 deutonymphs per P generation female irradiated with 270 Gy in ambient air was 4.0 ± 0.1, but the corresponding number that descended from females irradiated in pure oxygen was significantly reduced to zero. Percentage survival of females at 22 d post treatment was 13.0 ± 0.1 d when females were irradiated in oxygen with 270 Gy compared to 16 ± 0.2 d when irradiated in air with 300 Gy. Therefore, 300 Gy is recommended as an appropriate candidate for phytosanitary irradiation of B. yothersi in air, and 270 Gy is recommended as an appropriate candidate for phytosanitary irradiation of B. yothersi in oxygen.

    Palavras-Chave: gamma radiation; mites; oxygen; atmospheres; gamma sources; cobalt 60

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  • IPEN-DOC 26262

    FRANCO, SUELY S.H.; FRANCO, JOSE G. ; FERRARI, LUIZ; MACHI, ANDRE R. ; LEANDRO, RODRIGO S.R. ; ARTHUR, PAULA B. ; ARTHUR, VALTER . Propagation of mango seedlings of the cultivar “Tommy” by grafts irradiated. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 21-25, 2019, Santos, SP. Proceedings... Rio de Janeiro: Associação Brasileira de Energia Nuclear, 2019. p. 2157-2161.

    Abstract: Due to difficulties of vegetative propagating of any pants, many tests were performed with ionizing radiation aiming the modification of the characteristics of the plants for obtaining of the improved genotypes and of smaller size. Rootstock “Espada” cultivar, were used to obtain mango seedlings of “Tommy” cultivar. The cuttings were irradiated with different doses of gamma radiation: 0 (control), 2.5, 5.0, 7.5 and 10.0 Gy, in a source of Cobalt-60 type Gammacell-220, installed in the Center for Nuclear Energy in Agriculture, CENA-USP. After irradiation, was performed the grafting type cleft graft. Was made the first evaluation of height of the plants from the budding grafting after 24 months. Data were subjected by statistic program (SAS) and the means were compared by Tukey test (p <0.05). From the results obtained can be concluded that the doses of 2.5 Gy stimulated growth of the plants and the lethal dose was 10.0 Gy because don’t have development of plants.

    Palavras-Chave: cobalt 60; gamma radiation; grafts; irradiation; lethal doses; mangoes; radiation doses; seedlings

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  • IPEN-DOC 24149

    ARTHUR, VALTER ; FONTES, LUCIA da S.; ARTHUR, PAULA B. ; MACHI, ANDRE R. ; HARDER, MARCIA N.C.; ROSSI, RODRIGO S. ; FRANCO, JOSE G. ; FRANCO, SUELY S.H. . Quarantine treatment by gamma radiation for different development stages of Callosobruchus maculatus in bean Vigna sinensis. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 22-27, 2017, Belo Horizonte, MG. Proceedings... Rio de Janeiro, RJ: Associação Brasileira de Energia Nuclear, 2017.

    Abstract: The loss of stored grain caused by insects generates a problem of economic order of importance, due to concern about the increased supply of food for the world population is expanding. Associated with this fact, there is the problem of nutritional deficiency due to lack of protein, especially for the less privileged populations. The use of ionizing radiation in grains and products stored without a doubt can solve the problem of the losses in these products, since it does not induce resistance to insects and leaves no toxic residue in the products, and is considered an effective and safe method. The aim of the experiment was to determine the effect of ionizing radiation from cobalt-60 as a quarantine treatment for the different stages of development of Callosobruchus maculatus (Fabr., 1972) (Coleóptera, Chysomilidae) in bean Vigna sinensis. The experiment was conducted in the laboratory of Radiobiology and Environment CENA / USP., Piracicaba, SP, Brazil. Bean samples infested with eggs, larvae, pre-pupae and pupae C. maculatus, the experiment consisted of 4 replicates for each stage of the insect's life cycle, and each repetition consisted of 20 individuals (eggs, larvae, pre-pupae and pupae), a total of 200 subjects per treatment which were irradiated with doses of 0 (control), 25, 50, 75 and 100 Gy, a source of cobalt-60, Gammabeam-650 type, in a rate dose of 1.3 kGy / h. The experiment was conducted in a room with a relative of 25 ± 2 ° C temperature and humidity of 70 ± 5%. After 35 days of irradiation process were carried out evaluations of the number of insects emerged in each repetition within the treatments. From the results obtained it was concluded that the dose lethal to eggs and larvae was 25 Gy, while for pre-pupae was 50 Gy, to pupae 100 Gy was not sufficient to control the adult emergence.

    Palavras-Chave: beans; cobalt 60; dose rates; gamma radiation; insects; larvae; lethal doses; pupae; quarantine; radiation effects; radicidation

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  • IPEN-DOC 20814

    MACHI, ANDRE R. ; ESTECA, FERNANDA de C.N.; ARTHUR, PAULA B. ; GAVA, MARCIO A.; ARTHUR, VALTER. A review on Mononychellus tanajoa (Bondar, 1938) pest of cassava in Brazil. Australian Journal of Basic and Applied Sciences, v. 8, n. 3, p. 342-348, 2014.

    Palavras-Chave: brazil; cassava; mites; pest control

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  • IPEN-DOC 26260

    ARTHUR, PAULA B. ; VILLAVICENCIO, ANNA L.C.H. ; MACHI, ANDRE R. ; LEANDRO, RODRIGO S.R. ; ARTHUR, VALTER . Sterilizing of Alphitobius diaperinus (Panzer, 1797) (Coleoptera: Tenebrionidae) irradiated in pupa stage. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 21-25, 2019, Santos, SP. Proceedings... Rio de Janeiro: Associação Brasileira de Energia Nuclear, 2019. p. 2123-2128.

    Abstract: Worldwide the loss of stored grain is a problem of economic order of importance, in view of the concern of the increased supply of food for a world population increasingly expanding. Associated with this fact, there is the problem of nutritional deficiency due to lack of protein, especially for the less privileged populations in the resources of a country. This lack could be met by adequate supply of grain produced, requiring for it, a system that provides optimum grain storage conditions in securing the quality until the time of consumption. The use of radiation in stored grain can solve the problem of the losses in these products, as it does not induce resistance of insects and leaves no toxic residue to the consumer, and is considered an effective and safe method. The aim of the experiment was to determine the sterilizing dose of ionizing radiation from cobalt-60 to Alphitobius diaperinus (Panzer, 1797)(Coleoptera: Tenebrionidae) in peanuts irradiated in pupa stage. The experiment was conducted in the laboratory of Radiobiology and Environment of the Center for Nuclear Energy in Agriculture - CENA / USP., Piracicaba, SP, Brazil. Peanuts samples infested with pupae of A. diaperinus were utilized in the experiment that consisted of 8 treatments with 5 repetitions. Each repetition consisted of 20 pupae a total of 100 individuals per treatment. Were irradiated with doses of 0 (control), 25, 50, 75, 100, 125, 150 and 175 Gy, in a source of cobalt-60, Gammacell-220 type, with a rate dose of 381Gy / h. The experiment was conducted in a room with temperatures of 25 ± 5 ° C and relative humidity of 70 ± 5%. After of irradiation process was evaluated of the number of emerged adult insects in each repetition in the treatments. The results show that the sterilizing dose in F1 generation was 125 Gy and the pupa lethal dose 150 Gy. The dose of 150 Gy of gamma radiation can be used as phytosanitary treatment to control of immature stages of A. diaperinus infested peanuts.

    Palavras-Chave: beetles; cobalt 60; dose rates; insects; irradiation; peanuts; pupae; radiation dose units; radiation doses; sterilization

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  • IPEN-DOC 19922

    MACHI, ANDRE R. . Uso da radiação gama do cobalto-60 em ácaros de importância agrícola visando o tratamento quarentenário / Use of gamma radiation of cobalt-60 on mites in agricultural importance seeking treatment quarantine . 2014. Dissertação (Mestrado) - Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP, São Paulo. 54 p. Orientador: Valter Arthur. DOI: 10.11606/D.85.2014.tde-18032014-134742

    Abstract: O objetivo do trabalho foi estudar os efeitos da radiação gama nas fases do ciclo evolutivo de ácaros: Tetranychus urticae, Tetranychus desertorum, Oligonychus ilicis, visando o tratamento quarentenário como um método alternativo de controle. Para a realização do trabalho, ácaros das três espécies acondicionadas em bandejas plásticas medindo 20x15 cm circundadas por algodão hidrofóbico e cola entomológica (Stick®) para evitar uma possível fuga dos ácaros. Posteriormente, cada espécie foi colocada separadamente dentro de gaiolas de madeiras revestidas com tela tipo organdi (1,5 x 1,5 x 1,5m), e mantidos em casa de vegetação sob hospedeiro alternativo, plantas de Feijão de Porco (Canavalia ensiformis L) e folhas de café (Coffea arábica L) apenas para O. ilicis. Os ácaros foram identificados em um microscópio estereoscópico e transferidos com um pincel de ponta fina sobre placas de petri individualizadas com 1 acaro fêmea por placa (um total de 32 placas) contendo folhas de feijão de porco e folhas de café (C. arábica L). A irradiação dos ácaros femeas foram feitas em um irradiador com fonte Cobalto-60, tipo Gammacell-220, sob uma taxa de dose 0.486 kGy/hora, com doses de: 0 (controle) 100, 200, 300 e 400 Gy, em um total de 5 tratamentos e 32 repetições para cada ácaro. As avaliações foram realizadas a cada 24 horas, por um período de 22 dias após a irradiação, contando-se o número de: ovos, ninfas, adultos e mortalidade dos ácaros. Para as análises estatísticas foram utilizados modelos lineares generalizados com distribuição do tipo quase-binomial e gaussiana para análise dos dados de proporções de mortalidade e duração em dias das fases de ovos e ninfas das três espécies de ácaros envolvidas no estudo. A verificação da qualidade do ajuste dos dados foi feita por meio do uso do gráfico meio-normal de probabilidades com envelope de simulação. Quando houve diferença significativa entre os tratamentos, múltiplas comparações (teste de Tukey, P<0,05) foram realizadas por meio da função glht do pacote multicomp com ajuste dos valores de P. Todas as análises foram realizadas utilizando-se o software estatístico R, versão 2.15.1. A diminuição da viabilidade dos ovos e das ninfas dos ácaros foi diretamente proporcional ao aumento das doses de radiação gama. A dose de 200 Gy foi esterilizante para todas as fases do ciclo evolutivo de O.ilicis e 300 Gy para T.urticae e T. desertorum.

    Palavras-Chave: mites; pest control; gamma radiation; cobalt 60; quarantine

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A pesquisa no RD utiliza os recursos de busca da maioria das bases de dados. No entanto algumas dicas podem auxiliar para obter um resultado mais pertinente.

✔ É possível efetuar a busca de um autor ou um termo em todo o RD, por meio do Buscar no Repositório , isto é, o termo solicitado será localizado em qualquer campo do RD. No entanto esse tipo de pesquisa não é recomendada a não ser que se deseje um resultado amplo e generalizado.

✔ A pesquisa apresentará melhor resultado selecionando um dos filtros disponíveis em Navegar

✔ Os filtros disponíveis em Navegar tais como: Coleções, Ano de publicação, Títulos, Assuntos, Autores, Revista, Tipo de publicação são autoexplicativos. O filtro, Autores IPEN apresenta uma relação com os autores vinculados ao IPEN; o ID Autor IPEN diz respeito ao número único de identificação de cada autor constante no RD e sob o qual estão agrupados todos os seus trabalhos independente das variáveis do seu nome; Tipo de acesso diz respeito à acessibilidade do documento, isto é , sujeito as leis de direitos autorais, ID RT apresenta a relação dos relatórios técnicos, restritos para consulta das comunidades indicadas.

A opção Busca avançada utiliza os conectores da lógica boleana, é o melhor recurso para combinar chaves de busca e obter documentos relevantes à sua pesquisa, utilize os filtros apresentados na caixa de seleção para refinar o resultado de busca. Pode-se adicionar vários filtros a uma mesma busca.

Exemplo:

Buscar os artigos apresentados em um evento internacional de 2015, sobre loss of coolant, do autor Maprelian.

Autor: Maprelian

Título: loss of coolant

Tipo de publicação: Texto completo de evento

Ano de publicação: 2015

✔ Para indexação dos documentos é utilizado o Thesaurus do INIS, especializado na área nuclear e utilizado em todos os países membros da International Atomic Energy Agency – IAEA , por esse motivo, utilize os termos de busca de assunto em inglês; isto não exclui a busca livre por palavras, apenas o resultado pode não ser tão relevante ou pertinente.

✔ 95% do RD apresenta o texto completo do documento com livre acesso, para aqueles que apresentam o significa que e o documento está sujeito as leis de direitos autorais, solicita-se nesses casos contatar a Biblioteca do IPEN, bibl@ipen.br .

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✔ O RD disponibiliza um quadro estatístico de produtividade, onde é possível visualizar o número dos trabalhos agrupados por tipo de coleção, a medida que estão sendo depositados no RD.

✔ Na página inicial nas referências são sinalizados todos os autores IPEN, ao clicar nesse símbolo será aberta uma nova página correspondente à aquele autor – trata-se da página do pesquisador.

✔ Na página do pesquisador, é possível verificar, as variações do nome, a relação de todos os trabalhos com texto completo bem como um quadro resumo numérico; há links para o Currículo Lattes e o Google Acadêmico ( quando esse for informado).

ATENÇÃO!

ESTE TEXTO "AJUDA" ESTÁ SUJEITO A ATUALIZAÇÕES CONSTANTES, A MEDIDA QUE NOVAS FUNCIONALIDADES E RECURSOS DE BUSCA FOREM SENDO DESENVOLVIDOS PELAS EQUIPES DA BIBLIOTECA E DA INFORMÁTICA.

O gerenciamento do Repositório está a cargo da Biblioteca do IPEN. Constam neste RI, até o presente momento 20.950 itens que tanto podem ser artigos de periódicos ou de eventos nacionais e internacionais, dissertações e teses, livros, capítulo de livros e relatórios técnicos. Para participar do RI-IPEN é necessário que pelo menos um dos autores tenha vínculo acadêmico ou funcional com o Instituto. Nesta primeira etapa de funcionamento do RI, a coleta das publicações é realizada periodicamente pela equipe da Biblioteca do IPEN, extraindo os dados das bases internacionais tais como a Web of Science, Scopus, INIS, SciElo além de verificar o Currículo Lattes. O RI-IPEN apresenta também um aspecto inovador no seu funcionamento. Por meio de metadados específicos ele está vinculado ao sistema de gerenciamento das atividades do Plano Diretor anual do IPEN (SIGEPI). Com o objetivo de fornecer dados numéricos para a elaboração dos indicadores da Produção Cientifica Institucional, disponibiliza uma tabela estatística registrando em tempo real a inserção de novos itens. Foi criado um metadado que contém um número único para cada integrante da comunidade científica do IPEN. Esse metadado se transformou em um filtro que ao ser acionado apresenta todos os trabalhos de um determinado autor independente das variáveis na forma de citação do seu nome.

A elaboração do projeto do RI do IPEN foi iniciado em novembro de 2013, colocado em operação interna em julho de 2014 e disponibilizado na Internet em junho de 2015. Utiliza o software livre Dspace, desenvolvido pelo Massachusetts Institute of Technology (MIT). Para descrição dos metadados adota o padrão Dublin Core. É compatível com o Protocolo de Arquivos Abertos (OAI) permitindo interoperabilidade com repositórios de âmbito nacional e internacional.

1. Portaria IPEN-CNEN/SP nº 387, que estabeleceu os princípios que nortearam a criação do RDI, clique aqui.


2. A experiência do Instituto de Pesquisas Energéticas e Nucleares (IPEN-CNEN/SP) na criação de um Repositório Digital Institucional – RDI, clique aqui.

O Repositório Digital do IPEN é um equipamento institucional de acesso aberto, criado com o objetivo de reunir, preservar, disponibilizar e conferir maior visibilidade à Produção Científica publicada pelo Instituto, desde sua criação em 1956.

Operando, inicialmente como uma base de dados referencial o Repositório foi disponibilizado na atual plataforma, em junho de 2015. No Repositório está disponível o acesso ao conteúdo digital de artigos de periódicos, eventos, nacionais e internacionais, livros, capítulos, dissertações, teses e relatórios técnicos.

A elaboração do projeto do RI do IPEN foi iniciado em novembro de 2013, colocado em operação interna em julho de 2014 e disponibilizado na Internet em junho de 2015. Utiliza o software livre Dspace, desenvolvido pelo Massachusetts Institute of Technology (MIT). Para descrição dos metadados adota o padrão Dublin Core. É compatível com o Protocolo de Arquivos Abertos (OAI) permitindo interoperabilidade com repositórios de âmbito nacional e internacional.

O gerenciamento do Repositório está a cargo da Biblioteca do IPEN. Constam neste RI, até o presente momento 20.950 itens que tanto podem ser artigos de periódicos ou de eventos nacionais e internacionais, dissertações e teses, livros, capítulo de livros e relatórios técnicos. Para participar do RI-IPEN é necessário que pelo menos um dos autores tenha vínculo acadêmico ou funcional com o Instituto. Nesta primeira etapa de funcionamento do RI, a coleta das publicações é realizada periodicamente pela equipe da Biblioteca do IPEN, extraindo os dados das bases internacionais tais como a Web of Science, Scopus, INIS, SciElo além de verificar o Currículo Lattes. O RI-IPEN apresenta também um aspecto inovador no seu funcionamento. Por meio de metadados específicos ele está vinculado ao sistema de gerenciamento das atividades do Plano Diretor anual do IPEN (SIGEPI). Com o objetivo de fornecer dados numéricos para a elaboração dos indicadores da Produção Cientifica Institucional, disponibiliza uma tabela estatística registrando em tempo real a inserção de novos itens. Foi criado um metadado que contém um número único para cada integrante da comunidade científica do IPEN. Esse metadado se transformou em um filtro que ao ser acionado apresenta todos os trabalhos de um determinado autor independente das variáveis na forma de citação do seu nome.