Abstract: Carbon emissions from fires (C fire) account for one-tenth of the global annual C emissions. Fires are the main source of emissions from land-use change. Recently, Werf et al. 2017, showed an 11% increase in global fire emissions during the 1997-2016 period. Savannas and Tropical Forests have shared almost the same fire-derived C emission values in the South America (Werf et al. 2017). Therefore, for this study we focused our analysis on a site representing the transition zone between Amazonia and Cerrado, located in Alta Floresta (ALF) in Mato Grosso State, Brazil. To understand the temporal dynamics of fire-derived C emission, we correlated fire counts (FC) within the influence area with fire-derived C emission directly measured in the atmosphere as CO concentration. CO and CO2 were collected monthly for 7 years from 2010 onwards using an aircraft flying up to 4.5 km altitude, totaling 153 vertical profiles. FC was extracted from influence areas weighted by the density of backward trajectories calculated quarterly. Trajectories starting from the flight location within a cell of one degree resolution were obtained using the Hysplit model at different heights. The average annual C emission from fire was 0.10 ± 0.04 Pg.C.yr-1, which represents about 10% of the Amazon fire emissions (Aragão et al. 2018). The highest daily value of fire emission was observed in 2010 (drought year) and 2017 (0.47-0.51 gC.m².day-1), although the years of 2011, 2016, and 2017 were the largest contributor to the total emission flux. This occurred because the influence area that belongs to the Amazon was lower in those years. Inter annually there is a typical behavior of the backward trajectories in ALF site whose 1st and 4th quarters have 80% of the air-streams coming from the Amazon, while during the 2nd and 3rd quarters this contribution is about 45%. However, emissions from fire and FC are higher in the 3rd quarter, outside the Amazon. The correlation between FC and fire emission was positively significant (ρ = 0.88, α = 0.05, p<0.001), meaning that an increase of 1,000 FC per quarter causes an increase of 0.074 gC.m².day-1 or, on average, an emission of 0.16 Pg.C.yr-1.

Palavras-Chave: fires; carbon dioxide; emission; forests; greenhouse gases; environment

CASSOL, HENRIQUE L.G.; DOMINGUES, LUCAS G.; BASSO, LUANA S.; GATTI, LUCIANA V.; MARANI, LUCIANO; TEJADA, GRACIELA; ARAI, EGIDIO; MILLER, JOHN B.; ANDERSON, LIANA O.; ARAGAO, LUIZ E.O.C. Understanding the temporal dynamics of carbon emission from fires in the Amazon-Cerrado transition zone. Biodiversidade Brasileira, v. 9, n. 1, p. 132-132, 2019. DOI: 10.37002/biobrasil.v%25vi%25i.1273. Disponível em: http://200.136.52.105/handle/123456789/31671. Acesso em: $DATA.

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Abstract: Osteogenesis imperfecta (OI) is a congenital dysplasia of connective tissue characterized mainly by fragility and low bone density. The type I OI, a mild form of the disease, is associated with the quantitative decrease of type I collagen in the extracellular matrix, a characteristic also observed in the oim mice used in pre-clinical OI research. Previous studies have shown the efficiency of recombinant human growth hormone (GH) treatment for OI, both in animal and human models, reducing bone fragility, increasing bone density and stimulating α 1 and α 2 procollagen synthesis. This work aimed at using plasmid containing the murine GH (mGH) gene to treat oim heterozygous mice. Serological quantification of mGH by ELISA was carried out to evaluate the plasmid expression, bone density by DEXA and three-point flexion test were performed on the femurs to assess bone quality. In a short-term (3-day) trial, mGH levels of treated animals were 20.6±6.6 ng/mL, versus 3.3±2.2 ng/mL for those receiving saline and 2.3±1.3 ng/mL for the untreated wild-type group. A 90-day assay with plasmid applications at 0, 30 and 60 days was performed in parallel. The results of bone density showed greater effectiveness of the hormone during the first month of treatment, with a 34% increase in comparison with the saline group, whereas in the second month there was no significant statistical difference (p>0.05). The three-point bending test and the final analysis of bone density are currently being carried out.

JESUS, GUSTAVO P.P. de; GOMES, ALISSANDRA de M.; ZACARIAS, ENIO A.; YOSIDAKI, VANESSA de L.; BARTOLINI, PAOLO; PERONI, CIBELE N. Intramuscular growth hormone plasmid DNA eletrotransfer effects on bone quality in a murine model of osteogenesis imperfecta. Journal of Cell Science & Therapy, v. 10, p. 36-36, 2019. DOI: 10.4172/2157-7013-C2-053. Disponível em: http://repositorio.ipen.br/handle/123456789/30303. Acesso em: $DATA.

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Abstract: The use of fluoride products associated with high intensity laser irradiation are beneficial for dental caries prevention because it increases the surface area, improving the formation of fluorapatite (FA), which gives greater acid-resistance of enamel against bacterial acids. The objective of this study is to determine the critical pH of dental enamel treated with acid fluoride phosphate 12,300 lF-/g (APF) and Nd:YAG laser 84 J/cm2, as there is no precedent to determine this pH. The study consisted of 4 groups (n = 15): G1: Negative Control; G2: APF; G3: Nd:YAG; G4: APF + Nd:YAG. Each group was randomized into three subgroups (n = 5) for pH cycling. The cycling was designed to simulate three conditions: below critical pH of enamel hydroxyapatite (pH 5.0); pH below critical for hydroxyapatite and fluorapatite (pH 4.5); condition further below the critical situation to investigate extent of acid resistance of the enamel (pH 4.0). The samples were analyzed by scanning electron microscopy (SEM), Fourier infrared spectroscopy (FTIR) and solutions were quantified fluoride (specific ion electrode) and phosphate (colorimetric method). In SEM and FTIR (phosphate band) at pH 5, only the APF and APF + Nd:YAG groups did not present demineralization. At pH 4.5, only the APF + Nd:YAG group was integrated. At pH 4, APF + Nd:YAG showed signs of mild demineralization while the other groups showed aggressive signals. It is concluded that the irradiated fluorapatite has critical dissolution pH different from fluorapatite formed only with the application of fluorine.

Palavras-Chave: teeth; enamels; fluorides; phosphates; ph value; laser radiation

JUVINO, AMANDA C.; ZAMATARO, CLAUDIA B.; RABELO, THAIS F.; KUCHAR, NIELSEN G.; ZANINI, NATHALIA; CASTRO, PEDRO; ZEZELL, DENISE. In vitro determination of the critical pH demineralization of human dental enamel irradiated with Nd:YAG laser associated with fluoridated product. Photobiomodulation, Photomedicine, and Laser Surgery, v. 37, n. 10, p. A8-A9, 2019. DOI: 10.1089/photob.2019.29013.abstracts. Disponível em: http://repositorio.ipen.br/handle/123456789/32220. Acesso em: $DATA.

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Abstract: The role of tropical regions in the global CH4 balance remained uncertain, due these regions have until recently been poorly observed with large-scale integrating in-situ observations. To contribute in understanding the CH4 balance in tropical regions, we have started a lower-troposphere greenhouse gas-monitoring program over Brazilian Amazon Basin consisting of regular vertical profile greenhouse gas observations at four sites. Samples are collected regularly each 2 weeks, using light aircraft. We will present an analysis of these data and what they tell us about the Amazon CH4 cycle and its contribution to global CH4 concentration. We estimate fluxes upwind of the sites from the profile data using a column budgeting approach (Miller et al., 2007). Over the full period (2010-2017) the Amazon Basin was a source of CH4, but with regional variations. There are comparably high and highly variable emissions from the eastern part of the basin exhibiting strong variability, with particularly high CH4 fluxes in two different periods of the year (beginning of the wet season and in the dry season). In contrast to this, a clear seasonality was observed at the other three sites, with the largest emissions occurring at the beginning of the wet season (January to March). Emissions from biomass burning contribute with a small part of the total flux at each site. We will finally discuss what is the influence from precipitation and temperature in the Amazon CH4 emissions.

Palavras-Chave: methane; atmospheres; carbon monoxide; greenhouse gases; wetlands

BASSO, LUANA S.; GATTI, LUCIANA V.; MARANI, LUCIANO; CASSOL, HENRIQUE L.G.; TEJADA, GRACIELA; CORREIA, CAIO S.C.; DOMINGUES, LUCAS G.; CRISPIM, STEPHANE P.; NEVES, RAIANE A.L.; ARAI, EGIDIO; MILLER, JOHN B.; GLOOR, MANUEL; ANDERSON, LIANA O.; ARAGAO, LUIZ E.O.C. A first Amazon CH4 budget and its controls based on atmospheric data from vertical profiles measurements. Pesquisa Florestal Brasileira - Brazilian Journal of Forestry Research, v. 39, p. 528-528, 2019. Special issue, DOI: 10.4336/2019.pfb.39e201902043. Disponível em: http://200.136.52.105/handle/123456789/31669. Acesso em: $DATA.

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Abstract: Osteogenesis imperfecta (OI) is an inherited connective tissue disease characterized by fragility, deformity and low bone density, as well as by other clinical manifestations. Type I OI is the mildest and most common form of the disease, caused by mutation in the COL1A1 gene, resulting in the production of only ~50% of normal collagen. The corresponding animal model is the oim mouse, presenting a phenotype very similar to human type I OI. We aim, for the first time, at evaluating the electrotransference of mouse growth hormone (mGH) gene, encoding a protein that already showed therapeutic effects, together with the administration of murine mesenchymal stem cells (MSCs), for improving heterozygous oim mice phenotype. We already prepared and evaluated two populations of MSCs (bone marrow and adipose tissue) that emit red fluorescence. These are administrated in different amounts and via three routes (intravenously, intraperitoneally or locally into the femoral condyles) using the in vivo imaging system and histological sections of femur, liver and kidneys, a methodology we recently set up and adapted to our specific conditions. Then, we will administer the most efficient MSCs population combined with mGH gene electrotransfer. In this bioassay, we are ready to analyse different parameters: body weight, total body, tail and femur length, bone mineral density and femur fragility by a biomechanical flexion test. The results will indicate if the administration of GH by gene therapy, together with MSCs infusion, can be a promising treatment for improving type I OI phenotype.

GOMES, ALISSANDRA de M.; JESUS, GUSTAVO P.P. de; ZACARIAS, ENIO A.; YOSIDAKI, VANESSA L.; HIGUTI, ELIZA; RANGEL, ERIKA B.; BARTOLINI, PAOLO; PERONI, CIBELE N. Electrotransference of the mouse growth hormone gene associated with the administration of mesenchymal stem cells, in a murine model of osteogenesis imperfecta. Journal of Cell Science & Therapy, v. 10, p. 35-35, 2019. DOI: 10.4172/2157-7013-C2-053. Disponível em: http://repositorio.ipen.br/handle/123456789/30302. Acesso em: $DATA.

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Abstract: Porcelain laminated veneers have been widely used. For wear of hard tissue such as enamel and dentin, the diamond rotary instrument is the most traditional, but the laser has become recently used to remove aesthetic facets. Optical coherence tomography (OCT) used as an optical biopsy, is important for morphological analysis and attenuation coefficient is related to the property of the photons to be scattered by the samples. After approval by the Ethics Committee, the present study investigated the detachment of 30 ceramic E-max fragments cemented in human dental enamel of dimensions 3mm x 3mm x 0.7mm with 3 types of resin cements, RelxY Veneer, Relx U200 and Variolink Veneer. The samples (Enamel + Ceramic Fragment) were randomly distributed in the 3 groups and cemented according to the manufacturer. After that, they were prepared for irradiation with the Er,Cr: YSSG laser under predetermined conditions (3.5 and 3W, 20Hz, 60% water and 40% air flow). OCT analysis was done before and after irradiation. We observed that themorphological changes of the enamel surface showed an increased surface area due to the cement remaining in the enamel.We concluded that the Er, Cr: YSGG laser, when used in the irradiation protocol tested, seems to be a safe tool for the removal of laminates.

ZANINI, NATHALIA; ZAMATARO, CLAUDIA B.; RABELO, THAIS F.; JUVINO, AMANDA C.; KUCHAR, NIELSEN G.; CASTRO, PEDRO; ANA, PATRICIA da; ZEZELL, DENISE. Analysis of ceramic laminates removal with Er,Cr:YSGG laser by optical coherence tomography. Photobiomodulation, Photomedicine, and Laser Surgery, v. 37, n. 10, p. A22-A22, 2019. DOI: 10.1089/photob.2019.29013.abstracts. Disponível em: http://repositorio.ipen.br/handle/123456789/32224. Acesso em: $DATA.

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