Navegação Periódicos - Resumos por ano de publicação "2020"

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  • IPEN-DOC 27444

    SABINO, CAETANO P.; SELLERA, FABIO P.; SALES-MEDINA, DOUGLAS F.; MACHADO, RAFAEL R.G.; DURIGON, EDISON L.; FREITAS-JUNIOR, LUCIO H.; RIBEIRO, MARTHA S. . UV-C (254 nm) lethal doses for SARS-CoV-2. Photodiagnosis and Photodynamic Therapy, v. 32, p. 1-2, 2020. DOI: 10.1016/j.pdpdt.2020.101995

    Palavras-Chave: sterilization; germicides; radiation doses; ultraviolet radiation; coronaviruses

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  • IPEN-DOC 27563

    CARDOSO, ELIZABETH C.L. ; PARRA, DUCLERC F. ; SCAGLIUSI, SANDRA R. ; LUGAO, ADEMAR B. . Study of PBAT/PLA bio-based blends reinforced with chicken eggshell nano powder compatibilized with ionizing radiation. Astrophysics & Aerospace Technology, v. 8, n. 2, p. 11-11, 2020.

    Abstract: Eggshell is a solid waste, with production of several tons per day and it is mostly sent to landfills at a high management cost. A few used of eggshell include: fertilizing the garden, cleaning pots and pans, seed starter, feeding birds, making bone broth, etc… Nevertheless, chicken eggshell biowaste has recently been used to substitute calcium carbonate (CaCO3), due to its reinforcing property and low price. It is economically viable to transform eggshell waste to acquire new values, transforming it into a bioplastic: a biodegradable polymer made from bio-mass. The surprising strength of eggshells endorses their application for reinforcement of biodegradable polymers herein studied: PLA (poly-lactic acid) and PBAT (butylene adipate co-terephthalate). PLA is derived from renewable sourced: polylactic acid plastics are sturdier and capable to be blend with conventional petroleum polymers; nevertheless, they exhibit a narrow process window and low thermal stability, besides an inherent high cost. PBAT, as PLA, is a biodegradable aliphatic polyester, although a synthetic polymer based on fossil resources. By incorporating PBAT in PLA it is expected to improve flexibility of PBAT/PLA blend. Previous studies using PBAT/PLA, 50/50 blends containing 15% of chicken eggshell 125 µm improved mechanical behavior of net blends: values for both force and strain practically doubled, proving the effective reinforcement action of calcium carbonate extracted from eggshells. Conventional polymer processing methods can be used in both PLA and PBAT compositions. Due to common incompatibility between PLA and PBAT, considering their extreme glass transition temperatures: 62 º C and – 30 º C, respectively, it is required a compatibilizer to accomplish or increase their interaction. Herein it was used PLA previously e-beam radiated at 150 kGy, as compatibilizing agent: ionizing radiation induces compatibilization by free radicals, improving the dispersion and adhesion of blend phases, without the use of chemical additives and at room temperature. Herein there were prepared bio-composite PBAT/PLA 82/18 blends with 2.5, 5.0 and 10.0 % of eggshell nano-powder, 161 nm, in average, and 5.0 % of PLA 150 kGy e-beam radiated were homogeneized in a co-rotating twin-screw extruder. Subsequent investigations included: Differential Scanning Calorimeter (DSC), Thermal Gravimetric Analysis (TGA), Fourier Transmittance Infrared (FTIR), X-Ray Diffraction (XRD), Tensile Strength and Elongation at Break

    Palavras-Chave: eggs; chickens; fertilizers; solid wastes; ionizing radiations; nanopowders

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  • IPEN-DOC 26684

    PEREIRA, S.T. ; SILVA, C.R. ; RIBEIRO, M.S. . A single application of low-level light therapy in breast cancer-bearing mice: impact on blood cell count. Lasers in Medical Science, v. 35, n. 1, p. 274-274, 2020. DOI: 10.1007/s10103-019-02900-7

    Abstract: Low-level light therapy (LLLT) has been used in healthcare as a therapeutic strategy for diseases of various aetiologies. However, its effects on cancer is controversial. The aim of this work was to evaluate the effects of different light doses on blood cell count of breast cancer-bearing mice after a single application of LLLT. Tumour was induced by inoculation of 4T1 cells into the mammary fat pad of female BALB/c mice. Tumour volume was monitored with a digital calliper and when it reached 100 mm3, animals were irradiated by a red LED irradiator (660 ± 20 nm) at irradiance of 24.3 mW/cm2. The animals were randomly divided into groups (n= 5/group) that received energy densities of 1.8, 5.6 and 9.4 J/cm2. Control group was equally manipulated but did not receive irradiation. After treatment, data as tumour volume, platelets, white and red cell levels were registered weekly during 21 days. Our results showed that irradiated groups presented a longer survival compared to the control group, although no significant differences were observed among groups regarding tumour volume. Platelet levels of the irradiated groups remained closer to the reference values of healthy animals. Regarding the total number of white and red cells, no significant differences were observed between control and treated groups. These findings indicate that LLLT in a single application did not arrest tumour growth but may maintain platelet levels to increase survival of mice regardless the energy density protocol used.

    Palavras-Chave: blood cells; light emitting diodes; neoplasms; dose rates; blood platelets

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  • IPEN-DOC 26891

    YOSHIMURA, TANIA ; RIBEIRO, MARTHA S. . Photobiomodulation activates glucose uptake in brown adipose tissue of obese mice. Lasers in Medical Science, v. 35, n. 1, p. 280-280, 2020. DOI: 10.1007/s10103-019-02900-7

    Abstract: Mammal fat consists ofwhite and brown adipose tissues (WATand BAT). Although most fat is energy-storing WAT, brown adipocytes dissipate energy as heat. Obese subjects exhibit BAT hyporesponsiveness to classical stimuli as cold, hence increasing BAT thermogenic capacity could be an i n t er e s t i n g t a rget f o r t r eating obesity. Photobiomodulation (PBM) seems to be a feasible alternative to activate BAT, although it has not been applied for this purpose until now. In this study, adult C57Bl/6 mice were fed with a hyperlipidic diet (HF) during 15 weeks to induce obesity. Then, they were divided into two groups: HF and HF660. The interscapular BAT of HF660 mice was exposed to a single irradiation using a 662 ± 20 nm LED (5.7 Jcm-2 at 19 mWcm-2, delivered during 300 s). The HF group was sham-irradiated. Eutrophic animals were also used. Animals received 200 mCi of 18F-FDG and were exposed to cold for basal register of PET-CT images. After PBM or sh am treat ment, mice were once again exposed to cold before PET-CT image acquisition. Our results showed that PBM was able to activate BAT in obese mice through higher glucose uptake. In contrast, PBM and shamirradiated normal fed mice presented similar levels of glucose uptake. Taking together, our findings suggest that PBM applied to the BAT is able to promote glucose uptake by obese mice and could be an interesting approach to manage obesity and its correlated morbidities.

    Palavras-Chave: adipose tissue; glucose; metabolic diseases; mice; irradiation

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  • IPEN-DOC 26890

    NUNEZ, SILVIA C.; SILVA, IVES C. da; NASCIMENTO, JONATHAN S.; RIBEIRO, MARTHA S. . Methodological study of photobiomodulation anti-edematous effects on carrageenan-induced inflammation on zebrafish. Lasers in Medical Science, v. 35, n. 1, p. 288-289, 2020. DOI: 10.1007/s10103-019-02900-7

    Abstract: The anti-inflammatory action of Photobiomodulation (PBM) is one of its most reproducible effects in vivo. New models to study PBMeffects and to investigate light dosimetry may bring different perspectives on the field. Based on the importance of establishing new experimental models and the advantages of using zebrafish for screening new drugs with potential anti-inflammatory effects the present investigation evaluated the effect of PBM on carragenan-induced abdominal edema in zebrafish. Thirty Danio rerio (± 1 g) zebrafish were randomly divided into five aquariums with 0.25L of water to establish three treatments: T1 - Naive non-treated; T2 – Saline 0.9%-injected; T3 - carrageenan-injected (CG) 3.5% in the coelomic cavity. T4 –injected (CG) 3.5% and treated with a diode laser λ=808nm, 50mWand 0.5J T5 –same parameters with 1J and T6 -1.5J. Abdominal volumes were measured by a caliper and the area was calculated 4 hours post-indu ction. Four hours after edema induction the results revealed an increase in the abdominal area of fish injected with CG 3.5% compared to negative control. The animals injected with CG and treated with PBM exhibited a reduction in abdominal edema in a dose-dependent manner. The 0.5J energy did not present a significant reduction compare do control (p=0.18), the 1J and 1.5J groups presented a significant difference (p=0.027 and p=0.005 respectively). The 1.5J group was not different from negative control (T2) p=0.37. The present study identified that zebrafish may represent a good model to investigate PBM effects and the response was dose dependent.

    Palavras-Chave: inflammation; fishes; antipyretics; dosimetry; biological effects

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  • IPEN-DOC 28461

    BELLEZZO, M. ; FONSECA, G.P.; VONCKEN, R.; VERRIJSSEN, A.; VAN BEVEREN, C.; ROELOFS, E.; YORIYAZ, H. ; RENIERS, B.; VAN LIMBERGEN, E.J.; BARBEE, M.; VERHAEGEN, F.. MAASTRO applicator, a novel rectal applicator for contact brachytherapy with 192Ir HDR sources. Radiotherapy and Oncology, v. 152, Supplement 1, p. S1102-S1103, 2020. DOI: 10.1016/S0167-8140(21)01990-3

    Abstract: Purpose or Objective: The standard care for rectal cancer includes surgery, which may be avoided if complete response is achieved, e.g. with chemoradiotherapy (EBCRT) or external beam radiotherapy EBRT, adopting a watch and wait strategy. Studies report a local regrowth reduction from 30% (EBCRT alone) to 11% when EBCRT is associated with a radiation boost using 50 kV x-rays (CXB), technique that allows a high dose delivery to a highly selective volume, allowing preservation of organs at risk (OAR) and low toxicity. However, CXB is not widely adopted due to its low costeffectiveness. Hence, the MAASTRO applicator was developed to deliver a dose distribution similar to those generated by CXB devices, but using HDR 192Ir sources, as a cost-effective alternative to CXB, with possibility of integration to treatment planning systems (TPS). Material and Methods: Fig 1-a shows the applicator design, a cylindrical applicator with 5 channels and a slanted edge, using its tip. Results: Due to the applicator geometry, the most distal position of the source in each channel doesn’t reach the sharp edge of the applicator, resulting in an effective treatment surface (high dose region shown in Fig 1 a and b) of approximately 20 x 20 mm2, which is smaller than the contact surface. The resulting dose falloff is steeper than the one resulting from the P50 with a 22 mm applicator. With the dose falloff normalized at 2mm, the relative dose values delivered at depths of 0, 2, 5 and 10 mm are, respectively, 130, 100, 70 and 43% for the P50 and 140, 100, 67 and 38% for the applicator. The time required to deliver an average dose of 32 Gy to the treatment surface of the applicator is 5m30s for a 40700 U source (new source) and 8m30s for a 20350 U source (source to be replaced), including the time required to perform obstruction verification before irradiation. The applicator delivers a high dose to a small target volume while the lateral shielding spares normal tissues in all directions other than the contact surface. Conclusion: The MAASTRO applicator was designed to deliver dose distributions similar to those of CXB devices using 192Ir HDR sources. The applicator has the advantage of TPS integration, increasing the degrees of freedom to modulate the dose distribution.

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  • IPEN-DOC 27300

    WANG, RAYMOND Y.; FRANCO, JOSE F. da S. ; LOPEZ-VALDEZ, JAIME; MARTINS, ESMERALDA; SUTTON, VERNON R.; WHITLEY, CHESTER B.; ZHANG, LIN; CIMMS, TRICIA; MARSDEN, DEBORAH; JURECKA, AGNIESZKA; HARMATZ, PAUL. Long-term safety and efficacy of vestronidase alfa, rhGUS enzyme replacement therapy, in subjects with mucopolysaccharidosis type VII. Molecular Genetics and Metabolism, v. 129, n. 2, p. S158-S159, 2020. DOI: 10.1016/j.ymgme.2019.11.422

    Abstract: Vestronidase alfa (recombinant human beta-glucuronidase) is an enzyme replacement therapy for mucopolysaccharidosis type VII (MPS VII), a highly heterogeneous, ultra-rare disease. Twelve subjects, ages 8–25 years, completed a Phase 3, randomized, placebo-controlled, blind-start, single crossover study (UX003- CL301; NCT02230566), receiving 24–48 weeks of vestronidase alfa 4 mg/kg IV. All 12 subjects completed the blind-start study, which showed significantly reduced urinary glycosaminoglycans (uGAG) and clinical improvement in a multi-domain responder index, and enrolled in a long-term, open-label, extension study (UX003-CL202; NCT02432144). Here, we report the final results of the extension study, up to an additional 144 weeks after completion of the blindstart study. Three subjects (25%) completed all 144 weeks of study, eight subjects (67%) ended study participation before Week 144 to switch to commercially available vestronidase alfa, and one subject discontinued due to non-compliance after receiving one infusion of vestronidase alfa in the extension study. The safety profile of vestronidase alfa in the extension study was consistent with observations in the preceding blind-start study, with most adverse events mild to moderate in severity. There were no treatment or study discontinuations due to AEs and no noteworthy changes in a standard safety chemistry panel. There was no association between antibody formation and infusion associated reactions. Subjects receiving continuous vestronidase alfa treatment showed a sustained uGAG reduction and clinical response evaluated using a multidomain responder index that includes assessments in pulmonary function, motor function, range of motion, mobility, and visual acuity. Reductions in fatigue were also maintained in the overall population. Results from this study show the long-term safety and durability of clinical efficacy in subjects with MPS VII with long-term vestronidase alfa treatment.

    Palavras-Chave: recombinant dna; glucuronidase; mucopolysaccharides; glycoproteins; glycosides; enzymes; therapy

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  • IPEN-DOC 28465

    ETCHEBEHERE, E.; LIMA, M.; PEREIRA, L.; PAGNANO, R.; BORTOLETTI, E. ; MENGATTI, J. ; BRUNETTO, S.Q.; TAKAHASHI, M.; BRUNETTO, E.; OZELO, M.; SANTOS, A.. Knee radiosynovectomy with Sm-153 hydroxyapatite compared to Y-90 hydroxyapatite: initial results of a prospective trial. European Journal of Nuclear Medicine and Molecular Imaging, v. 47, Supplement 1, p. S628-S628, 2020. DOI: 10.1007/s00259-020-04988-4

    Abstract: Aim/Introduction: Introduction: The most common clinical presentation in hemophilia patients consists of hemarthrosis. Various treatment strategies aim to control hemarthrosis to prevent secondary arthropathy, among them, radiosynovectomy with Y-90 hydroxyapatite (90YHA). A few studies have shown a lower efficiency of knee radiosynovectomy with Sm-153 hydroxyapatite (153Sm- HA) compared to 90Y-HA. Purpose: The purpose of this investigation was to assess the efficacy and safety of knee radiosynovectomy with 153Sm-HA compared to 90Y-HA. Materials and Methods: Forty patients were prospectively assigned to undergo knee radiosynovectomy with 153Sm- HA (19 patients) or with 90Y-HA (21 patients). The frequency of hemarthrosis episodes before and after treatment was compared. Results: The response to knee radiosynovectomy stratifying according to radiotracer showed that after 6 months the median response rate with 153Sm-HA was not significantly different from 90Y-HA (87.5% vs 80.9%; p = 0.576). However, after 12 months the median response rate of knee radiosynovectomy with 153Sm-HA was significantly better than with 90Y-HA (87.5% vs 50%; p = 0.037), respectively. The reduction of joint bleeding by at least 50%, after 12 months, was greater in the group of patients treated with 153Sm-HA compared to 90Y-HA (74% vs 52%), respectively. Conclusion: Knee radiosynovectomy with high doses of 153Sm-HA is safe, with an efficiency rate similar that is described in the literature by the 90Y-HA.

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  • IPEN-DOC 27446

    VILLAS BOAS, CRISTIAN A.W. ; MENGATTI, JAIR ; PASSOS, PRISCILA ; VIEIRA, DANIEL ; ARAUJO, ELAINE B. de . In vitro response of 177Lu-PSMA-617 with two different specific activities. Journal of Nuclear Medicine, v. 61, supplement 1, 2020.

    Abstract: Introduction: PSMA-617 radiolabeled with lutetium-177 has shown good results in compassionate studies around the world. Being a receptor-specific radiopharmaceutical, the specific activity (SA) of the preparation may represent an important factor for therapeutic efficacy. Lutetium-177 can be produced by two different routes: with ytterbium-176 (Non-carrier-added or NCA) and with lutetium-176 (Carrier-added or CA). The SA (MBq/ug) of the labeled PSMA varies accordingly to each lutetium. For NCA lutetium, the radiolabeling procedure is based on the SA of 74 MBq/ug. When the radiolabeling is performed with CA lutetium, SA is determined by the molar ratio of 2.1:1 (PSMA moles/lutetium moles declared in the certificate), resulting in lower SA than NCA. This work evaluated the influence of specific activity of 177Lu-PSMA-617 on in vitro specific binding assays (saturation, competition and internalization). Materials and Methods: Radiolabeling of PSMA-617 (ABX, Germany) with lutetium-177 was performed in heating block at 90°C for 30 minutes with sodium ascorbate (0.5 M pH 4.7) as buffer. For NCA lutetium (JSC, Russia) the radiopharmaceutical specific activity was 74 MBq/ug. For CA lutetium (IDB, Netherlands), the specific activity was 41 MBq/ug. The radiochemical purity was analyzed with HPLC. For all experiments, 6-well plates were used for adherence cells with 200,000 LNCaP per well. Molar concentration of saturation curves experiments were 0.01; 0.05; 0.6; 1.5; 3.0 and 3.5 for CA lutetium and 0.1; 0.6; 1.5; 2.0; 2.5 and 3.0 for NCA lutetium. After 1 hour of incubation at 8 ºC, supernatant was removed, then washed with PBS (phosphate buffer saline) and finally cells were burst with NaOH 1 M, and activity was measured in gama counter; the experiments were performed in octuplicate. Competition experiments were performed adding in all wells 5 nM of radiolabeled PSMA-617 and in the competition well (non-specific binding) were added an excess of 15 times (76 ug) of non radiolabeled PSMA-617. After 1 hour of incubation at 8 ºC, supernatant was removed, then washed with PBS and finally cells were burst with NaOH 1 M, and activity was measured in gama counter, these experiments were performed in triplicate. The specific binding was obtained by the difference between total binding and non-specific binding. Internalization experiments were performed at Kd of NCA and CA lutetium. After 1 hour of incubation at 37 ºC, supernatant was removed, washed with PBS, then washed again with 0.05 M glycine solution pH 2.8 and finally cells were burst with NaOH 1 M. Activity was measured in gama counter, these experiment were performed in sextuplicate. Results and discussion: The radiochemical purity were 98% and 99% for labeling with NCA and CA lutetium, respectively. Saturation curve assay with NCA lutetium shown a Kd of 0.7 ± 0.15 nM and a Bmax of 857 ± 55.79 pMol/ng, and with CA lutetium resulted in a Kd of 1.71 ± 0.45 nM and a Bmax of 1156 ± 113.8 pMol/ng. The variation between both Kd curves were statistically different (P value = 0.0058). Competition assay demonstrated an effective blocking for both types of lutetium, for NCA unpaired T test shown a P value of 0.0011. For CA lutetium, the unpaired test disclosed a P value of 0.0258. The comparison between both results revealed a P value of 0.01 at the specific binding. Internalization assay shown for both types of lutetium similar results, 27.1 ± 2.45% and 30.6 ± 4.97%, for CA and NCA lutetium, respectively, and was not statistically significant (P value = 0.17). Conclusions: These experiments demonstrated that the type of lutetium (CA or NCA) directly affects in vitro binding of 177Lu-PSMA-617 to receptors expressed in LNCaP cells. It was statistically demonstrated that the higher specific activity of 177Lu-PSMA-617, more radiolabeled peptide can bind to cells at saturation and competition assays.

    Palavras-Chave: radiopharmaceuticals; lutetium 177; therapy; in vitro

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  • IPEN-DOC 27445

    COELHO, GUILHERME R.; PREZOTTO NETO, JOSE P. ; BARBOSA, FERNANDA C.; SANTOS, RAFAEL S.; BRIGATTE, PATRICIA; SPENCER, PATRICK J. ; SAMPAIO, SANDRA C.; D'AMELIO, FERNANDA; PIMENTA, DANIEL C.; SCIANI, JULIANA M.. Hypanus americanus mucus: a new point of view about stingray immunity and toxins. Toxicon, v. 177, Supplement 1, p. S34-S34, 2020. DOI: 10.1016/j.toxicon.2019.12.047

    Abstract: Fish skin plays important biological roles, such as the control of the osmotic pressure gradient, protection against mechanical forces and microorganism infections. The mucus, on the other hand, is a rich and complex fluid, important for the fish acting as innate immunity system, swimming and nutrition. The elasmobranch epidermis is characterized mainly by mucus secretory cells, and marine stingrays have already been described to present secretory glands spread throughout the body. Little is known about the biochemical composition of the stingray mucus, but recent studies denoted the importance of mucus in the envenomation process. Stingrays venom are largely studied due the human medical importance of envenoming caused by sting puncture, that evolve with local inflammation and necrosis, and these toxic events can be correlated to the chemical composition of the sting skin, according to the literature. Aiming to analyse the mucus composition, a new non-invasive mucus collection method was developed that focused on peptides and proteins, and biological assays were performed to analyze preliminary toxic and immune activities of the Hypanus americanus mucus. Pathophysiological characterization showed the presence of peptidases on mucus, as well that the induction of edema and leukocyte recruitment in mice. The fractionated mucus improved phagocytosis on macrophages and showed antimicrobial activity against T. rubrum, C. neoformans and C. albicans in vitro. The proteomic analyses showed the presence of immune-related proteins like actin, histones, hemoglobin, and ribosomal proteins. This protein pattern is similar to those reported for other fish mucus and stingray venom. This is the first report depicting the Hypanus stingray mucus composition, highlighting its biochemical composition and importance for the stingray immune system and the possible role on the envenomation process.

    Palavras-Chave: fishes; skin; venoms; therapeutic uses

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  • IPEN-DOC 26889

    SILVA, C.R. ; RIBEIRO, M.S. . Effects of photobiomodulation on breast tumor- bearing mice before radiotherapy. Lasers in Medical Science, v. 35, n. 1, p. 269-269, 2020. DOI: 10.1007/s10103-019-02900-7

    Abstract: Photobiomodulation (PBM) has been studied to modify the cellular response to ionizing radiation. However, its combination with radiotherapy (RT) has not been reported in cancer treatment. The aim of this study was to evaluate the effects of PBM applied before RT on breast tumor-bearing mice. Female BALB/c mice were inoculated with breast 4T1 cells into mammary fat pad and divided into 4 groups (n =5 per group): control (with no treatment), only RT, and PBM combined to RT in two different protocols. RT was locally applied using a 60Co source with dose of 60 Gy in fractions of 15 Gy. For PBM, a red laser (660 nm, 500 mW/cm2) was used in two regimes: single exposure 24 h before RT (fluence of 150 J/cm2) and immediately before each RT session (fluence of 37.5 J/cm2 per session). After treatment, tumor volume, platelets, white and red blood cell levels were evaluated during 14 days. Our results showed no statistically significant differen ces in t umor volume, platelet and red blood cell levels comparing control, RT and PBM+RT groups. However, PBM was able to sustain normal white blood cell levels compared to RT and control groups. In addition, mice that received PBM concomitant with RT presented a longer survival. In fact, for this group only 12.5 % of the animals died during experimental period. These findings indicate that PBM could be combined to RT to provide therapeutic anti-cancer benefits.

    Palavras-Chave: ionizing radiations; therapy; neoplasms; tumor cells; mice; mammary glands; radiotherapy

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  • IPEN-DOC 26888

    CONTATORI, C.G. de S. ; SILVA, C.R. ; YOSHIMURA, T.M. ; RIBEIRO, M.S. . Effects of low-level laser irradiation on VEGF expression of melanoma cell lines. Lasers in Medical Science, v. 35, n. 1, p. 269-269, 2020. DOI: 10.1007/s10103-019-02900-7

    Abstract: Impact of low-level laser irradiation on tumor cell lines remains controversial. Vascular endothelial growth factor (VEGF) is a key molecule to form new blood vessels, which contribute for cancer development and growth. The aim of this study was to evaluate the effects of different light fluences on human melanoma SKMEL 37 cells and murine melanoma B16F10 cells using a near infrared laser (λ= 780 nm) with output power of 40 mW delivering energies of 1.2, 3.6 and 6 J (fluences of 30, 90 and 150 J/cm2, respectively). The cell lines were irradiated 24 h after they were seeded in a 96-well plate at a density of 5x103 cells per well, in triplicate at three different days. Following irradiation, both cell line supernatants were stored in Eppendorf tubes at - 20°C until VEGF-A expression measurement. Specific ELISA kits were used according to cell line (murine or human). Samples and standard solutions were added in a 96-well plate antibody-coated and incuba ted over night at 4°C. Reagent dilution and set time followed fabricant instructions. The stop solution was added and the absorbance was read in a microplate reader at 450 nm. Results showed a non-statistically significant difference among treated and control groups for both cell lines. These findings indicate that irradiation with near infrared laser does not influence VEGF expression on melanoma cell lines regardless the fluence used and should be tested to prevent cancer growth in preclinical assays.

    Palavras-Chave: laser radiation; low dose irradiation; doses; irradiation; tumor cells; melanomas; enzyme immunoassay

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  • IPEN-DOC 27301

    KADLUBOWSKI, SLAWOMIR S.; VARCA, GUSTAVO H.C. . Editorial. Radiation Physics and Chemistry, v. 169, SI, 2020. DOI: 10.1016/j.radphyschem.2019.108542

    Palavras-Chave: nanoparticles; radiations; nanomaterials; electron beams

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  • IPEN-DOC 27604

    PARRA, DUCLERC F. ; SANTOS, VINICIUS J. dos ; TAMIÃO, ANGELICA . Development of poly (n-vinyl-2-pyrrolidone) hydrogels containing nanosilver and laponite clay for modulation of neomycin. Journal of Astrophysics & Aerospace Technology, v. 8, n. 2, p. 3-3, 2020.

    Abstract: These Biomaterials widely used in biomedicine as wound dressings are composed by hydrogels. They offer ideal conditions for wound treatment, such as maintaining the wound environment humid, autolytic debridement, accelerated healing, ability to absorb large amounts of fluids without losing their physical integrity, drug release in a controlled manner protecting infected wounds that are constantly exposed to environment contaminated with microorganisms. These dressings are formed by one or more polymeric components which, when cross-linked, form three-dimensional networks. They have a high degree of flexibility that is very similar to natural living tissues. The general objective of this work was to synthesize by radiation hydrogel membranes containing PVP, PEG, agar, RD laponite in a neomycin and nanosilver release system that promotes a synergistic action between these two antimicrobial agents. Membranes with different concentrations of polymer matrix / clay were produced by irradiation at a dose of 25 kGy of gamma 60Co source, and characterized by techniques: swelling analysis, thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), tensile tests, parallel plate rheometry. It can be seen that G 'and G "are approximately parallel within the studied range. G 'is greater than G' 'and both showed little frequency dependence. This performance is the expected for the gels, in which the elastic behavior of the sample predominates over its viscous behavior. The clay-polymer interactions increases gel cross-linking, which provides an increase in their mechanical properties. In parallel, nanoparticles of silver coupled to clay (Laponite) were synthesized in which their properties were verified by means of UV-Vis, MET, ICP-OES and microbiological activity. The study of the microbiological activity of AgNPs-Laponite nanoparticles on the reduction of microorganisms in culture indicated that most of the nanoparticles, synthesized from the reduction of silver ions on the surface of Laponite, have activity against Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Candida albicans.

    Palavras-Chave: wounds; hydrogels; pvp; healing; biological recovery

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  • IPEN-DOC 28464

    RAMOS, C.; SOUZA, S.; TOBAR, N.; CASTRO, V.; FRASSON, F.; AMORIM, B.; ETCHEBEHERE, E.; LIMA, M.; MENGATTI, J. ; ARAUJO, E. ; PERINI, E. ; SOUZA, C.; LORAND-METZE, I.; SANTOS, A.; DELAMAIN, M.. Comparison of 68Ga-PSMA and 18F-FDG PET/CT uptake in different lymphoma subtypes: preliminary results. European Journal of Nuclear Medicine and Molecular Imaging, v. 47, Supplement 1, p. S41-S42, 2020. DOI: 10.1007/s00259-020-04988-4

    Abstract: Aim/Introduction: Few reports have documented the uptake of radiolabeled Prostate-Specific Membrane Antigen (PSMA) in lymphomas [1,2]. It is not known how PSMA uptake varies among various histological subtypes and how it correlates with 18F-FDG uptake in lymphomas. This study aimed to compare 68Ga-PSMA and 18F-FDG in different lymphoma subtypes. Materials and Methods: Nine randomly selected patients with biopsy-proven lymphoma -median age 43 (32-70) years, 5 female - were submitted to whole-body 18F-FDG and 68Ga- PSMA PET/CT (time interval: 1-6 days between procedures). Lymphoma subtypes included: nodular-sclerosis Hodgkin’s lymphoma (HL; 2 patients); diffuse large B-cell lymphoma (DLBCL; 1); marginal-zone lymphoma (2); MALT lymphoma (ML; 1); follicular lymphoma (FL; 1); lymphoplasmacytic lymphoma (1); and B-cell non- Hodgkin’s lymphoma, unspecified (BCNHL-U; 1). Eight patients were under initial staging, and 1 (HL) with disease relapse after treatment. Two experienced nuclear physicians analyzed the images by consensus. The intensity of tracer uptake was visually classified as marked, moderate or mild. The affected sites (lymph node chains, spleen, diffuse bone marrow involvement and non-lymphatic focal lesions) were counted in both sets of images and their respective maximum SUV (SUVmax) were measured. Results: PSMA PET/ CT was positive in all patients except for one with ML. FDG PET/CT was positive in all patients. At visual analyses, FDG uptake was higher than PSMA uptake in all patients, except for one patient with BCNHL-U (both tracers with similar low-intensity uptake). The intensity of FDG and PSMA uptake was respectively classified as marked in 3/9 and 0/8 patients, moderate in 4/9 and 1/8 and mild in 2/9 and 7/8. One patient (FL) presented a “mismatch” uptake pattern with different parts of an extensive lesion presenting predominant uptake of PSMA or FDG. Brain infiltration in one patient (DLBCL) was more easily identified on PSMA than on FDG images. FDG detected a total of 58/58 and PSMA 43/58 affected sites in all patients with a median SUVmax of respectively 5.4 (2.0-31.1) and 2.8 (1.3-5.4), p<0.0001. The median SUVs of the 43 lesions with uptake of both tracers was respectively 5.5 (2.0-28.9) and 2.8 (1.3-P5.4) for FDG and PSMA, p<0.0001. Conclusion: Distinct lymphoma subtypes present PSMA uptake, with less intensity than FDG uptake. Although PSMA uptake is usually mild, several lymphoma subtypes might cause false-positive results in PSMA PET/CT performed to assess prostate cancer.

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  • IPEN-DOC 28463

    SOUZA, S.; DELAMAIN, M.; TOBAR, N.; CASTRO, V.; FRASSON, F.; AMORIM, B.; ETCHEBEHERE, E.; MARIANA, K.; MENGATTI, J. ; ARAUJO, E.B. ; PERINI, E. ; SOUZA, C. de; SANTOS, A.; LORANT-METZE, I.; RAMOS, C.. Comparison of 68ga-psma and 18f-fdg pet/ct uptake in different lymphoma. Hematology, Transfusion and Cell Therapy, v. 42, Supplement 1, p. S48-S48, 2020. DOI: 10.1016/j.htct.2020.09.086

    Abstract: Objective: Few reports have documented the uptake of radiolabeled Prostate-Specific Membrane Antigen (PSMA) in lymphomas.1, [2] It is not known how PSMA uptake varies among various histological subtypes and how it correlates with 18F-FDG uptake in lymphomas. This study aimed to compare 68Ga-PSMA and 18F-FDG in different lymphoma subtypes. Methodology: Nine randomly selected patients with biopsy-proven lymphoma with a median age 43 (32–70) years, 5 female – were submitted to whole-body 18F-FDG and 68Ga – PSMA PET/CT (time interval: 1–6 days between procedures). Lymphoma subtypes included: nodular-sclerosis Hodgkin's lymphoma (HL; 2 patients); diffuse large B-cell lymphoma (DLBCL; 1); marginal-zone lymphoma (2); MALT lymphoma (ML; 1); follicular lymphoma (FL; 1); lymphoplasmacytic lymphoma (1); and B-cell non-Hodgkin's lymphoma, unspecified (BCNHL-U; 1). Eight patients were under initial staging and 1 (HL) with disease relapse after treatment. Two experienced nuclear physicians analyzed the images by consensus. The intensity of tracer uptake was visually classified as marked, moderate or mild. The affected sites (lymph node chains, spleen, diffuse bone marrow involvement and non-lymphatic focal lesions) were counted in both sets of images and their respective maximum SUV (SUVmax) were measured. Results: PSMA PET/CT was positive in all patients except for one with ML. FDG PET/CT was positive in all patients. At visual analyses, FDG uptake was higher than PSMA uptake in all patients, except for one patient with BCNHL-U (both tracers with similar low-intensity uptake). The intensity of FDG and PSMA uptake was respectively classified as marked in 3/9 and 0/8 patients, moderate in 4/9 and 1/8 and mild in 2/9 and 7/8. One patient (FL) presented a “mismatch” uptake pattern with different parts of an extensive lesion presenting predominant uptake of PSMA or FDG. Brain infiltration in one patient (DLBCL) was more easily identified on PSMA than on FDG images. FDG detected a total of 58/58 and PSMA 43/58 affected sites in all patients with a median SUVmax of respectively 5.4 (2.0–31.1) and 2.8 (1.3–5.4), p < 0.0001. The median SUVs of the 43 lesions with uptake of both tracers was respectively 5.5 (2.0–28.9) and 2.8 (1.3–5.4) for FDG and PSMA, p < 0.0001. Conclusion: Distinct lymphoma subtypes present PSMA uptake, with less intensity than FDG uptake. Although PSMA uptake is usually mild, several lymphoma subtypes might cause false-positive results in PSMA PET/CT performed to assess prostate cancer.

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  • IPEN-DOC 28462

    ANGELOCCI, L. ; NOGUEIRA, B.R. ; SOUZA, C.D. de ; ZEITUNI, C.A. ; ROSTELATO, M.E.C.M. . Assessing Ir-192 as an alternative to I-125 in ophthalmic treatment. Radiotherapy and Oncology, v. 152, Supplement 1, p. S735-S736, 2020. DOI: 10.1016/S0167-8140(21)01402-X

    Abstract: Purpose or Objective: Brachytherapy sources for ocular melanoma usually contain Co-60, I-125, Pd-103 or Ru/Rh-106 as radionuclides. Ir-192 is not a preconized radioactive material for this purpose, although it is used for other brachytherapy applications. Higher mean energy from Ir-192 emission (ca. 380 keV) may be a reason for the preference of I-125 (35 keV) or Pd-103 (21 keV) over it, since low penetration is desired on the small structures of the human eye. This is not, however, an excluding criterion, considering Co-60 and Ru/Rh-106 have even higher mean energies. The demand in Brazil for lower-cost seeds to treat ocular melanoma lead to the development of an Ir-192 seed to make treatment more accessible, but since it is not used as an ophthalmic brachytherapy source, before its dosimetry is considered, one should care about the possibility of using it over more stablished materials. Considering this, the aim of this work is to assess the possibility of using Ir-192 seeds as ophthalmic brachytherapy sources by comparing some dosimetric parameters of a new seed model with the most stablished I-125 seed in literature, OncoSeed 6711. Material and Methods: As an initial study on the topic, this work relies only on Monte-Carlo simulations using MCNP4C transport code. Parameters analyzed are air-kerma strength, dose-rate constant and depth-dose curve, attention given to points within the human eye dimensions. The medium considered was homogeneous water, as it is a good approximation to the eye tissues in terms of composition and density and allows for future comparisons with TG-43 based calculations. OncoSeed 6711 is not produced anymore, but its long term as the reference source for dosimetry was considered. A 20 mm COMS ophthalmic applicator was also modeled and considered to be fully loaded with each seed model to compare the same parameters at a realistically clinical approach. Results: As expected, due to the higher energy of the Ir-192 emission spectrum, dose fall-off on the transversal axis of the seeds is less pronounced for the new seed model. The steeper dose gradient for I-125 is also visible on the doserate constant value. The effect of using a COMS applicator only strengthens this characteristic. Depth-dose curves were calculated up to the distance of 5 cm, both for a single seed and for an applicator fully loaded with 24 seeds. All the eye components relevant for dosimetry are located within this range, like the cells of the crystallin and the optical nerve. Conclusion: If one expects to use Ir-192 as an alternative to I-125 in ophthalmic cancer treatment, at least the dosimetry following TG-43 protocol should be carried with utmost attention, as undesirable dose to healthy nearby tissues is unavoidable. Crafting a different applicator most suited for this radionuclide is a possibility that can be taken into account. Another recommendation is to go beyond TG-43 water-based protocol and actually estimate dose to relevant eye components.

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A elaboração do projeto do RI do IPEN foi iniciado em novembro de 2013, colocado em operação interna em julho de 2014 e disponibilizado na Internet em junho de 2015. Utiliza o software livre Dspace, desenvolvido pelo Massachusetts Institute of Technology (MIT). Para descrição dos metadados adota o padrão Dublin Core. É compatível com o Protocolo de Arquivos Abertos (OAI) permitindo interoperabilidade com repositórios de âmbito nacional e internacional.

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O Repositório Digital do IPEN é um equipamento institucional de acesso aberto, criado com o objetivo de reunir, preservar, disponibilizar e conferir maior visibilidade à Produção Científica publicada pelo Instituto, desde sua criação em 1956.

Operando, inicialmente como uma base de dados referencial o Repositório foi disponibilizado na atual plataforma, em junho de 2015. No Repositório está disponível o acesso ao conteúdo digital de artigos de periódicos, eventos, nacionais e internacionais, livros, capítulos, dissertações, teses e relatórios técnicos.

A elaboração do projeto do RI do IPEN foi iniciado em novembro de 2013, colocado em operação interna em julho de 2014 e disponibilizado na Internet em junho de 2015. Utiliza o software livre Dspace, desenvolvido pelo Massachusetts Institute of Technology (MIT). Para descrição dos metadados adota o padrão Dublin Core. É compatível com o Protocolo de Arquivos Abertos (OAI) permitindo interoperabilidade com repositórios de âmbito nacional e internacional.

O gerenciamento do Repositório está a cargo da Biblioteca do IPEN. Constam neste RI, até o presente momento 20.950 itens que tanto podem ser artigos de periódicos ou de eventos nacionais e internacionais, dissertações e teses, livros, capítulo de livros e relatórios técnicos. Para participar do RI-IPEN é necessário que pelo menos um dos autores tenha vínculo acadêmico ou funcional com o Instituto. Nesta primeira etapa de funcionamento do RI, a coleta das publicações é realizada periodicamente pela equipe da Biblioteca do IPEN, extraindo os dados das bases internacionais tais como a Web of Science, Scopus, INIS, SciElo além de verificar o Currículo Lattes. O RI-IPEN apresenta também um aspecto inovador no seu funcionamento. Por meio de metadados específicos ele está vinculado ao sistema de gerenciamento das atividades do Plano Diretor anual do IPEN (SIGEPI). Com o objetivo de fornecer dados numéricos para a elaboração dos indicadores da Produção Cientifica Institucional, disponibiliza uma tabela estatística registrando em tempo real a inserção de novos itens. Foi criado um metadado que contém um número único para cada integrante da comunidade científica do IPEN. Esse metadado se transformou em um filtro que ao ser acionado apresenta todos os trabalhos de um determinado autor independente das variáveis na forma de citação do seu nome.