Navegação Periódicos - Resumos por Autores IPEN "RIBEIRO, M.S."

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  • IPEN-DOC 11770

    YAMADA JUNIOR, A.M. ; HAYEK, R.R.A.; GIOSO, M.A.; FERREIRA, J.; BATISTA SOBRINHO, C.A.; RIBEIRO, M.S. . Bacterial reduction by photodynamic therapy in peri-implantitis: an in vivo study. Brazilian Dental Journal, v. 15, Special issue, p. 84-84, 2004.

    Abstract: Progressive peri-implantar bone losses, which are accompanied by inflammatory process in the soft tissues is referred to as peri-implantitis. The aim of this study was to compare the effects of lethal photosensitization with the conventional technique on bacterial reduction in ligature induced peri-implantitis in dogs. Seventeen third premolars of eight Labrador dogs were extracted and, immediately after, the implants were submerged. After osseointegration, peri-implantitis was induced. After 4 months, ligature were removed and the same period was waited for natural induction of bacterial plaque. The dogs were randomly divided into two groups. In the conventional group, they were treated with the conventional techniques of mucoperiosteal flaps for scaling the implant surface and irrigation with chlorexidine. In the laser group, only mucoperiosteal scaling was carried out before photodynamic therapy. On the peri-implantar pocket an azulene paste was introduced and a GaAlAs low-power laser (l= 660 nm, P= 30 mW, E= 5,4 J and Dt= 3 min.) was applied. Microbiological samples were obtained before and immediately after treatment. The results of this study showed that Prevotella sp., Fusobacterium e S. Beta-haemolyticus were significantly reduced for the conventional and laser groups (100%,99.8%; 100%,100%; 85.7%,97.6%, respectively).

    Palavras-Chave: dentistry; teeth; implants; bone tissues; bacteria; photodynamic therapy; lasers; photosensitivity

  • IPEN-DOC 14592

    SUZUKI, L.C.; YAMADA JUNIOR, A.M. ; HAYEK, R.R.A.; RIBEIRO, M.S. . A case report of photodynamic therapy on bacterial reduction before immediate implant. Brazilian Dental Journal, v. 15, Special issue, p. 127-127, 2004.

    Abstract: Recent studies have demonstrated that a number of oral bacteria can be killed by photodynamic therapy with low concentrations of dyes. Photodynamic therapy is the combination of light with appropriate wavelength and a photosensitizer. The antimicrobial activity is mainly mediated by singlet oxygen and/or free radicals generated by the photoactivated sensitizer. A flap surgery aiming an immediate implant was made in a residual root with periodontal lesion on upper first premolar. After the extraction, a microbiological sample with sterile paper points was harvested. Then, the photosensitizer was applied in the infected alveolus and irradiated with low-intensity laser, ?= 660 nm, P= 30 mW and E= 9 J . After the photodynamic therapy, a new microbiological sample was harvested. Subsequently, it was prepared the implant bed with conical burs and then the implant was placed. Patient was medicated with antibiotic after surgery. The microbiological analysis showed a significant reduction of Prevotella sp., Fusobacterium sp. and Streptococcus beta-hemoliticus. This finding suggests that photodynamic therapy is an alternative method to disinfect alveolus before implant placement. Therefore, this study highlights the need for future work in the area of photodynamic therapy to reduce bacteria without harming host tissue.

  • IPEN-DOC 10875

    HAYEK, R.R.A.; YAMADA JUNIOR, A.M. ; GARCEZ, A.S.; NUNEZ, S.C. ; SUZUKI, L.C.; RIBEIRO, M.S. . Comparation between photodynamic therapy and a bactericidal solution in the treatment of dental alveolitis microbiological evaluation. Brazilian Dental Journal, v. 15, Special issue, p. 110-110, 2004.

    Abstract: Photodynamic therapy is a new therapeutic modality to treat microbial infections. The aim of this pilot study was to compare this new therapeutic approach with clorhexidine in mini pigs induced alveolitis. Alveolitis is an inflammatory disease that involves the superficial bone layer in the dental alveolus. Eight dental elements were extracted from mini pigs and immediately after the alveolus were contaminated with Streptoccocus aureus. After seven days the area presented edema and hyperemia. In the clorhexidine group the alveolus were debrided and irrigated with a 0.12% solution of clorhexidine. In Laser group the alveolus were debrided and photodynamic therapy was performed consisting in the application of a azulene paste inside the alveolus with a preirradiation time of 5 minutes followed by irradiation with a low power diode laser, ?= 685 nm, P= 50 mW, E= 9 J, for 3 minutes. Microbiological samples were harvested before and after treatment for both groups with sterile paper points. The results showed bacterial reduction in both groups, although photodynamic therapy was significantly more effective to reduce the population of S. aureus. This finding indicates that photodynamic therapy can be an alternative method to the treatment of alveolitis.

    Palavras-Chave: dentistry; photodynamic therapy; germicides; inflammation

  • IPEN-DOC 11228

    KOHARA, E.K. ; CRUZ, D.R.; WETTER, N.U. ; RIBEIRO, M.S. . Effects of low-intensity laser therapy on the Orthodontic movement velocity of human teeth: a clinical study. Brazilian Dental Journal, v. 15, Special issue, p. 84-84, 2004.

    Abstract: Low-intensity laser therapy (LILT) has been studied in many fields of Dentistry, but, to our knowledge, it is the first time that its effects on orthodontic movement velocity in humans are investigated. In our study, eleven patients were recruited for a two-month study. One half of the upper arcade was considered control group and received mechanical activation of the canine teeth every thirty days. The opposite half received the same mechanical activation and was also irradiated with a diode laser (?=780nm) on ten points around the root, during 10s with 20 mW, 5 J/cm2, on four days of each month. Data of the biometrical progress of both groups were statistically compared. All patients showed significant higher retraction velocity of the canines on the laser treated side when compared to the control. Conclusion: Our findings suggest that LILT does accelerate human teeth movement and could therefore considerably shorten the whole treatment duration.

    Palavras-Chave: teeth; orthodontic treatments; semiconductor lasers; light emitting diodes; therapy; bone tissues

  • IPEN-DOC 12992

    GODOY, B.M.; ARANA-CHAVEZ, V.E.; BORTOLI GROTH, E.B.; RIBEIRO, M.S. . Effects of low-intensity red laser radiation on the dentine-pulp interface after class I cavity preparation disfunction. Brazilian Dental Journal, v. 15, Special issue, p. 86-87, 2004.

    Abstract: The aim of this study was to investigate the effects of low-intensity red laser radiation on the ultrastructure of dentine-pulp interface after conventionally prepared class I cavity preparation. Eight premolars indicated for extraction for orthodontic reasons from 2 patients were used. Class I cavities were prepared and the teeth were divided into two groups. The first group received a treatment with a GaAlAs laser, l= 660 nm, P= 30 mW and D= 2J/cm2. The laser tip was applied directly and perpendicularly into the cavity in only one sense. The teeth from the second group had their class I cavities prepared but they did not receive the laser therapy. All cavities were filled with composite resin. Twenty-eight days after the preparation, the teeth were extracted and processed for transmission electron microscopy analysis. Two sound teeth (healthy group) without any preparation were also examined. The first group presented odontoblastic processes in intimate contact with the extracellular matrix, while the collagen fibers appeared more aggregated and organized than those of the second group. These results were also observed in the healthy teeth. The results suggest that laser irradiation accelerates the recovery of the structures at the dentine-pulp interface involved during cavity preparation layer.

    Palavras-Chave: teeth; dentin; oral cavity; lasers; radiation effects

  • IPEN-DOC 26888

    CONTATORI, C.G. de S. ; SILVA, C.R. ; YOSHIMURA, T.M. ; RIBEIRO, M.S. . Effects of low-level laser irradiation on VEGF expression of melanoma cell lines. Lasers in Medical Science, v. 35, n. 1, p. 269-269, 2020. DOI: 10.1007/s10103-019-02900-7

    Abstract: Impact of low-level laser irradiation on tumor cell lines remains controversial. Vascular endothelial growth factor (VEGF) is a key molecule to form new blood vessels, which contribute for cancer development and growth. The aim of this study was to evaluate the effects of different light fluences on human melanoma SKMEL 37 cells and murine melanoma B16F10 cells using a near infrared laser (λ= 780 nm) with output power of 40 mW delivering energies of 1.2, 3.6 and 6 J (fluences of 30, 90 and 150 J/cm2, respectively). The cell lines were irradiated 24 h after they were seeded in a 96-well plate at a density of 5x103 cells per well, in triplicate at three different days. Following irradiation, both cell line supernatants were stored in Eppendorf tubes at - 20°C until VEGF-A expression measurement. Specific ELISA kits were used according to cell line (murine or human). Samples and standard solutions were added in a 96-well plate antibody-coated and incuba ted over night at 4°C. Reagent dilution and set time followed fabricant instructions. The stop solution was added and the absorbance was read in a microplate reader at 450 nm. Results showed a non-statistically significant difference among treated and control groups for both cell lines. These findings indicate that irradiation with near infrared laser does not influence VEGF expression on melanoma cell lines regardless the fluence used and should be tested to prevent cancer growth in preclinical assays.

    Palavras-Chave: laser radiation; low dose irradiation; doses; irradiation; tumor cells; melanomas; enzyme immunoassay

  • IPEN-DOC 26889

    SILVA, C.R. ; RIBEIRO, M.S. . Effects of photobiomodulation on breast tumor- bearing mice before radiotherapy. Lasers in Medical Science, v. 35, n. 1, p. 269-269, 2020. DOI: 10.1007/s10103-019-02900-7

    Abstract: Photobiomodulation (PBM) has been studied to modify the cellular response to ionizing radiation. However, its combination with radiotherapy (RT) has not been reported in cancer treatment. The aim of this study was to evaluate the effects of PBM applied before RT on breast tumor-bearing mice. Female BALB/c mice were inoculated with breast 4T1 cells into mammary fat pad and divided into 4 groups (n =5 per group): control (with no treatment), only RT, and PBM combined to RT in two different protocols. RT was locally applied using a 60Co source with dose of 60 Gy in fractions of 15 Gy. For PBM, a red laser (660 nm, 500 mW/cm2) was used in two regimes: single exposure 24 h before RT (fluence of 150 J/cm2) and immediately before each RT session (fluence of 37.5 J/cm2 per session). After treatment, tumor volume, platelets, white and red blood cell levels were evaluated during 14 days. Our results showed no statistically significant differen ces in t umor volume, platelet and red blood cell levels comparing control, RT and PBM+RT groups. However, PBM was able to sustain normal white blood cell levels compared to RT and control groups. In addition, mice that received PBM concomitant with RT presented a longer survival. In fact, for this group only 12.5 % of the animals died during experimental period. These findings indicate that PBM could be combined to RT to provide therapeutic anti-cancer benefits.

    Palavras-Chave: ionizing radiations; therapy; neoplasms; tumor cells; mice; mammary glands; radiotherapy

  • IPEN-DOC 14214

    NAKASONE, R.K.; RODRIGUES, C.R.M.D.; BUZALAF, M.A.R.; OLYMPO, K.P.K.; TANJI, E.Y.; RIBEIRO, M.S. . Effects of the low-intensity red laser radiation on the fluoride uptake in human enamel: a preliminary study. Brazilian Dental Journal, v. 15, Special issue, p. 119-120, 2004.

    Abstract: Fluoride has been the most important preventive method on development of the caries. This in vivo study evaluated the effects of lowintensity red laser radiation on the fluoride uptake in enamel. Ten healthy participants were recruited for this study. The two upper central incisors of each volunteer were used and divided into 4 groups: group GC (control), which was untreated; group GF (fluoride), which received topical acidulated phosphate fluoride (APF) 1,23% treatment for 4 minutes; group GLF (laser + fluoride), which was irradiated with a low-intensity diode laser (l= 660 nm and dose= 6 J/cm²) with APF application after irradiation and group GFL (fluoride + laser), which received APF before irradiation using the same parameters as GLF. The determination of fluoride was performed using a fluoride ion electrode after an acid-etch enamel biopsy. The results showed a significant increase of the fluoride uptake in enamel for groups GF, GLF and GFL when compared to control group. Although a percentage increase of 57% was observed for GLF with respect to GF, there were no statistical differences among the treated groups. These findings motivate further studies in order to introduce low-power laser associated to APF as a protective method of dental caries.

  • IPEN-DOC 26931

    CABRAL, F.V. ; SILVA, C.R. ; RIBEIRO, M.S. . Estudo da terapia fotodinâmica no tratamento de leishmaniose cutânea em um modelo murino. Anais da SBBN, v. 3, p. 57-57, 2016.

    Abstract: Introdução: Leishmanioses são doenças infecto-parasitárias, de evolução crônica, desenvolvidas por protozoários do gênero Leishmania. A forma cutânea abrange lesões destrutivas e ulceradas com diversas limitações no tratamento. O surgimento de terapias alternativas é fundamental devido à grande relevância da doença e elevada incidência. Nesse contexto, a terapia fotodinâmica (PDT) tem sido introduzida como possibilidade terapêutica, em função do baixo custo, mínima toxicidade, praticidade e sem relatos de resistência na literatura. Objetivos:Avaliar os efeitos da PDT no tratamento de leishmaniose cutânea induzida em camundongos BALB/c. Métodos: Este estudo foi aprovado pelo CEUA/IPEN sob o no. 70/10.Culturas de L.(L.)amazonensis(IFLA/BR/67/PH8) foram mantidas a 25oC em meio Grace com 10% de soro fetal bovino. Para a infecção, foram utilizados 16 camundongos BALB/c com 8 semanas e massa aproximada de 30g. Foram inoculados 1x106parasitos no membro posterior esquerdo dos animais, que foram monitorados por 4 semanas. O tamanho de ambos os membros de cada animal foi mensurado com um paquímetro, semanalmente. Após a quarta semana de infecção, os animais foram submetidos à PDT (azul de metileno a 100 μM, λ= 660 nm) e divididos em 4 grupos (n=4) de acordo com a densidade de energia utilizada: 50 J/cm2(G50), 100 J/cm2(G100), 150 J/cm2(G150) e grupo controle (GC=animais infectados e não tratados). Os animais foram anestesiados (quetamina, 90mg/kg e xilazina,10mg/kg) e acompanhados durante 3 semanas pós-PDT para avaliar a evolução da lesão, dor e carga parasitária. Para estimar a altura da lesão, a diferença entre os dados obtidos da pata infectada e contralateral sem infecção foi calculada e normalizada. A avaliação da dor foi obtida através do método von Frey, que é um teste de pressão crescente utilizado em animais. Uma escala de dor de 1 a 6 foi estipulada (1= pouca e 6= grande sensibilidade). Para a carga parasitária, o tecido da lesão foi macerado, filtrado, centrifugado, ressuspendidoem meio e semeado em placas de 96 poços e realizada a diluição limitante até o último poço, com fator de diluição 10. A placa foi incubada a 25ºC por 7 dias. A análise estatística foi obtida no programa Prism6, com os testes Anova Two-WayeTukey. Os resultados foram considerados significativos quando p<0,05. Resultados:Os resultados indicaram que houve diferença estatística no tamanho da lesão entre os grupos GC e G150 (GC= 259,3%23,6, G150= 175%19,1), (GC= 292,1%51, G150= 195,3%32,1) 2 e 3 semanas após a PDT, respectivamente. Na escala de dor, observamos diferenças significativas entre GC e G150 (GC= 5,750,5, G150= 4,250,5), (GC= 6, G150= 1,250,5) após 2 e 3 semanas, respectivamente e GC e G100 após 3 semanas (GC= 6, G100= 4,250,5). Porém, nos resultados da carga parasitária não foi observada diferença significativa. Conclusão:O presente estudo mostrou que a terapia fotodinâmica reduziu o tamanho da lesão e aliviou a dor em leishmaniose cutânea induzida em camundongos, sugerindo que a PDT pode ter influenciado no processo inflamatório. Estes resultados encorajam mais estudos para compreensão dos mecanismos da PDT no tratamento de leishmaniose cutânea.

    Palavras-Chave: parasitic diseases; skin diseases; parasites; photosensitivity; therapy; photochemistry

  • IPEN-DOC 26932

    SILVA, C.R. ; LUNA, A.C.L.; MARIA, D.A.; RIBEIRO, M.S. . Fotobiomodulação em células de câncer de mama após exposição à radiação ionizante. Anais da SBBN, v. 3, p. 56-56, 2016.

    Abstract: Introdução: A radioterapia vem sendo utilizada no tratamento de alguns tipos de câncer, causando alguns efeitos deletérios a células sadias adjacentes. A fotobiomodulação(PBM) surge como uma alternativa para modular processos inflamatórios e acelerar a cicatrização de lesões, no entanto, seu uso na Oncologia é limitado já que os efeitos da PBM em células tumorais são controversos. Objetivos: O objetivo deste estudo foi verificar os efeitos da PBM em células de câncer de mama após exposição à radiação gama. Métodos: As células de câncer de mama (MDA-MB-231) foram cultivadas em meio DMEM suplementado com 10 % de soro fetal bovino e armazenadas em incubadora com 5% CO2 a 37C. Uma concentração de células (1x105) foi colocada em placas de 96 poços em triplicata e exposta à radiação ionizante em um irradiador de fonte 60CO tipo Gamacellcom a dose de 10Gy(IR10). Vinte e quatro horas após a radiação ionizante, as células foram expostas à irradiação de um laser de emissão λ= 660nm, potência de saída de 40mW e área de 0,04cm². A distância entre o laser e a monocamada de células foi mantida constante de modo que o laser ficasse em contato direto com o fundo da placa. O tempo de exposição foi de 60 s (IR10+PMB60) e 120 s (IR10+PMB120), correspondendo às energias de 2,4 e 4,8J (PMB), respectivamente. Após vinte e quatro horas da exposição ao laser, foi verificada a viabilidade celular através do teste de exclusão com azul de tripane contagem em hemocitômetro, o ciclo celular, expressão de pcna, caspase3 e a proteína p53 utilizando a técnica de citometriade fluxo com canal de leitura em FL1-H do grupo não irradiado com radiação gama e não irradiado com laser (IR0+PMB0) e dos demais grupos. Os experimentos foram realizados em triplicata em três momentos distintos (n=9). A análise estatística foi realizada no programa OriginPro 8com os testes Shapiro Wilkpara testar normalidade, Anova One-Way para comparação das médias. O teste deTukeyfoi realizado para identificar diferenças significativas quando p<0,05. Resultados: Os resultados obtidos mostraram que durante o período experimental analisado, a PBM não influenciou na viabilidade celular (IR0+PMB0=25,95±1,07, IR10= 24,84±5,87, IR10+PMB60=26,11±1,69, IR10+PMB120=21,72±1,56, PMB= 23,45±0,33), na expressão de caspase3(IR0+PMB0=1,7±0,8,IR10=1,25±0,07,IR10+PMB60=1,00±0,30,IR10+PMB120=2,45±0,15, PMB= 1,55±0,75) e da proteína p53 (IR0+PMB0=5,35±1,75, IR10= 6,1±1,32,IR10+PMB60= 5,9±0,05, IR10+PMB120=6,35±1,15, PMB= 6,35±1,15), independente da energia utilizada. No ciclo celular foi possível verificar maior população nas fases S e G2/m, entretanto a expressão de pcna(IR0+PMB0=14,85±0,77,IR10=8,65±0,91,IR10+PMB60=4,35±0,85, IR10+PMB120=6,45±1,55, PMB= 6,0±0,8) não foi significativa, mas apresentou valores inferiores comparados ao grupo IR10. Conclusões: Em vista dos resultados apresentados verificamos que a PBM não influenciou a viabilidade celular, as expressões de caspase3, p53 e a expressão de pcna, independente da energia utilizada. Estes resultados sugerem que a PBM pode ser associada ao tratamento dos efeitos deletérios da radioterapia em pacientes oncológicos.

    Palavras-Chave: cobalt 60; gamma radiation; healing; inflammation; lambda particle beams; laser radiation; mammary glands; radiation effects; tumor cells; viability

  • IPEN-DOC 14036

    MULLER, K.P. ; YAMADA JUNIOR, A.M. ; SUGAYAMA, S.T.; RIBEIRO, M.S. . Local Treatment using high and low-power laser on herpes simplex. Brazilian Dental Journal, v. 15, Special issue, p. 119-119, 2004.

    Abstract: Herpes simplex (HSV) is commonly encountered in the dentistry practice disappearing after 10 to 14 days. Recently alternative treatments have been introduced, among those laser therapy. The aim of this study is to evaluate the efficacy of laser therapy in patients with perioral herpes simplex infection. A 32 years old female was treated with high-intensity laser therapy (HILT) in vesicle phase using a Nd:YAG laser, l= 1064 nm, P= 1,5 W, f= 15 Hz on days 1 and 2 to drainage the vesicles. Low-intensity laser therapy (LILT) was applied on the affected area in the 3 consecutive days with a GaAlAs laser, l= 830 nm, D= 5 J/cm2. On the other hand, a 7 years male patient, which presented the vesiculo-ulcerative stage, only LILT (l= 660 nm, D= 4 J/cm2) was carried out. For both cases, it was observed that the course of the infection was quicker than the normal. Seven days after treatment beginning the lesions were healed. These findings suggest that LILT associated or not to HILT is a efficient and promising treatment for HSV. Further larger studies aiming at confirming these data and investigating the LILT mechanisms of action should be performed.

  • IPEN-DOC 01757

    EDUARDO, L.R.P.; RIBEIRO, M.S. ; DUARTE, M.; ZEZELL, D.M. . Low level laser therapy in treatment of TMJ and masticator muscles disease: biometrical and pain evaluation. Brazilian Dental Journal, v. 15, Special issue, p. 87-87, 2004.

    Abstract: A sample of 11 patients showing temporomandibular joint disease, in one or both sides, was selected. Clinical examinations were performed on patients to define which side was the most compromised by the disease. Only the worst side was chosen to be treated by the laser therapy. The Laser Unit used was a diode laser (AlGaAs) in which the wavelength was 810 nm (infra-red spectrum). Irradiations were done in tree points of TMJ, with 22,5 J/cm2; two points in the masseter muscle, with 15 J/cm2; and tree points in the temporal muscle, with 7,5 J/cm2. All points were irradiated for 30 seconds. The other side, of lesser complaint received a sham therapy. Treatment was done during two weeks, tree irradiation with 48 hours interval in the first week, and two irradiations with the same interval in the second week. Patients reported improvement in relation to the level of pain, mainly after the forth irradiation, and the level of pain decreased until the last day of treatment. In relation to the degree of mouth opening, the majority of patients showed a significant increase, mainly after the fifth day of irradiation.

    Palavras-Chave: laser radiation; therapy; muscles; jaw; pain

  • IPEN-DOC 13067

    LOLLATO, R.F.; GROTH, E.B.; RIBEIRO, M.S. . Low-intensity laser therapy and functional orthopedics in the treatment of pain from temporomandibular disfunction. Brazilian Dental Journal, v. 15, Special issue, p. 114-114, 2004.

    Abstract: Temporomandibular Disfunction (TMD) is a term used to describe disorders, which involve temporomandibular joint (TMJ), masticatory muscles, and associated structures, isolated or not, whose most frequent symptom is pain. The aim of this study was to evaluate pain and buccal mobility in subjects with Cl II malocclusion and TMD symptoms, treated with low-intensity laser therapy (LILT) and functional orthopedics (FO), associated or not. Eighteen subjects were selected and divided into three groups. Group 1 was treated with LILT, l = 780nm, 70 mW, 15 J/cm2 per point during two weeks. The application was in three points around the TMJ, and trigger points of masticatory muscles. Group 2 received Planas functional appliances and it was evaluated once a week during two weeks. Group 3 received both treatments at the same time, and the first laser application coincided with the appliance installation. Subjects answered a questionnaire with a score for pain evaluation after palpation. There was pain relief for the 3 groups and no statistical differences were observed among them, however, group 3 showed a remission of pain faster than other groups. This finding indicates the association of the LILT with FO for pain relief in TMD.

  • IPEN-DOC 12376

    NUNEZ, S.C. ; GOMES, L.; GARCEZ, A.S.; MULLER, K.P. ; JORGE, A.O.C.; RIBEIRO, M.S. . Photodynamic action of toluidine blue in streptococcus mutans by fluorescence spectroscopy. Brazilian Dental Journal, v. 15, Special issue, p. 84-85, 2004.

    Abstract: The antimicrobial activity of toluidine blue associated with red light has been demonstrated for a wide range of microorganisms including those commonly found in infected root canals, carious lesions and periodontal pockets. Recent reports have drawn attention to the problems of antimicrobial resistance and resistance of oral bacteria to antibiotics and local antiseptics is of increasing concern, thus photodynamic therapy could be an alternative antimicrobial approach to treat localized infections in oral cavity. In this study the fluorescence spectra of TB were obtained before and after laser exposure in the presence or absence of Streptococcus mutans. The dye concentration was 0.01%, the irradiation was performed with a diode laser, l= 660 nm, P= 40mW, exposure time of 3 minutes in a volume of 0.5 ml, with a pre-irradiation time (PIT) of one or five minutes. The results showed shifts in fluorescence spectra observed for different preirradiation times in the presence of S. mutans. In the absence of bacteria, a shift in the spectra was observed in the dye before and after irradiation. These findings may indicate a photobleaching of the dye denoting structural alterations after irradiation and confirm the importance of the PIT for the success of this therapy.

    Palavras-Chave: dentistry; caries; bacteria; antimicrobial agents; toluidine blue

  • IPEN-DOC 21607

    SABINO, C.P. ; NEVES, P.R.; LINCOPAN, N.; RIBEIRO, M.S. . Photodynamic therapy inhibits the antimicrobial effects of bacteriophage therapy in a invertebrate model of systemic infection. Photodiagnosis and Photodynamic Therapy, v. 12, n. 3, p. 357, 2015. DOI: 10.1016/j.pdpdt.2015.07.127

    Palavras-Chave: photosensitivity; antimicrobial agents; invertebrates; bacteriophages; viruses; pseudomonas; in vitro; therapy

  • IPEN-DOC 15946

    BAPTISTA, A. ; KATO, I.T. ; PRATES, R.A. ; FREITAS, A.Z. ; RIBEIRO, M.S. ; AMARAL, M.M. . Redução bacteriana através da terapia fotodinâmica na doença cárie. Estudo in vivo. Brazilian Oral Research, v. 24, n. Supl. 1, p. 368, 2010.

    Palavras-Chave: teeth; caries; antimicrobial agents; photosensitivity; therapy

  • IPEN-DOC 26684

    PEREIRA, S.T. ; SILVA, C.R. ; RIBEIRO, M.S. . A single application of low-level light therapy in breast cancer-bearing mice: impact on blood cell count. Lasers in Medical Science, v. 35, n. 1, p. 274-274, 2020. DOI: 10.1007/s10103-019-02900-7

    Abstract: Low-level light therapy (LLLT) has been used in healthcare as a therapeutic strategy for diseases of various aetiologies. However, its effects on cancer is controversial. The aim of this work was to evaluate the effects of different light doses on blood cell count of breast cancer-bearing mice after a single application of LLLT. Tumour was induced by inoculation of 4T1 cells into the mammary fat pad of female BALB/c mice. Tumour volume was monitored with a digital calliper and when it reached 100 mm3, animals were irradiated by a red LED irradiator (660 ± 20 nm) at irradiance of 24.3 mW/cm2. The animals were randomly divided into groups (n= 5/group) that received energy densities of 1.8, 5.6 and 9.4 J/cm2. Control group was equally manipulated but did not receive irradiation. After treatment, data as tumour volume, platelets, white and red cell levels were registered weekly during 21 days. Our results showed that irradiated groups presented a longer survival compared to the control group, although no significant differences were observed among groups regarding tumour volume. Platelet levels of the irradiated groups remained closer to the reference values of healthy animals. Regarding the total number of white and red cells, no significant differences were observed between control and treated groups. These findings indicate that LLLT in a single application did not arrest tumour growth but may maintain platelet levels to increase survival of mice regardless the energy density protocol used.

    Palavras-Chave: blood cells; light emitting diodes; neoplasms; dose rates; blood platelets

  • IPEN-DOC 13005

    ARCHILLA, J.R.F.; TANNOUS, J.T.; PELINO, J.E.P.; FREITAS, P.M.; SILVA, D.F.T.; RIBEIRO, M.S. . Spectroscopic analysis and interaction of different bleaching agents with 660n laser and 470 nm LED. Brazilian Dental Journal, v. 15, Special issue, p. 100-100, 2004.

    Abstract: The aim of this in vitro study was to verify the effect of different dental bleaching methods regarding the achromatic enamel color change. Forty-five bovine teeth were immersed in a darkening solution and then divided into nine experimental groups with five samples in each group. The color measurement was initially taken using a digital colorimeter. The teeth were submitted to the bleaching with three bleaching gels, without any activation source (control) and with two wavelengths (660 nm diode laser and 470 nm LED). The previous analysis of the absorption spectra enabled the choice of an adequate wavelength in order to enhance its photochemical action. The second color measurement was taken after all the treatments. The data were submitted to ANOVA and Tukey parametric statistical tests (p=0,05). It was observed that: 1) there was a statistical significant difference between the activation sources; 2) there was statistical significant difference regarding the interaction among the activation sources and the bleaching gels when compared with each other. The activation methods of the bleaching agents, even promoting color changes in distinct levels, still require further studies capable of detecting a higher absorption spectrum of a product and which presents significant clinical results.

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A elaboração do projeto do RI do IPEN foi iniciado em novembro de 2013, colocado em operação interna em julho de 2014 e disponibilizado na Internet em junho de 2015. Utiliza o software livre Dspace, desenvolvido pelo Massachusetts Institute of Technology (MIT). Para descrição dos metadados adota o padrão Dublin Core. É compatível com o Protocolo de Arquivos Abertos (OAI) permitindo interoperabilidade com repositórios de âmbito nacional e internacional.

O gerenciamento do Repositório está a cargo da Biblioteca do IPEN. Constam neste RI, até o presente momento 20.950 itens que tanto podem ser artigos de periódicos ou de eventos nacionais e internacionais, dissertações e teses, livros, capítulo de livros e relatórios técnicos. Para participar do RI-IPEN é necessário que pelo menos um dos autores tenha vínculo acadêmico ou funcional com o Instituto. Nesta primeira etapa de funcionamento do RI, a coleta das publicações é realizada periodicamente pela equipe da Biblioteca do IPEN, extraindo os dados das bases internacionais tais como a Web of Science, Scopus, INIS, SciElo além de verificar o Currículo Lattes. O RI-IPEN apresenta também um aspecto inovador no seu funcionamento. Por meio de metadados específicos ele está vinculado ao sistema de gerenciamento das atividades do Plano Diretor anual do IPEN (SIGEPI). Com o objetivo de fornecer dados numéricos para a elaboração dos indicadores da Produção Cientifica Institucional, disponibiliza uma tabela estatística registrando em tempo real a inserção de novos itens. Foi criado um metadado que contém um número único para cada integrante da comunidade científica do IPEN. Esse metadado se transformou em um filtro que ao ser acionado apresenta todos os trabalhos de um determinado autor independente das variáveis na forma de citação do seu nome.