Abstract: Over the last few years, the trend of using flowers in gastronomy, edible flowers are used in food preparations in order to add beauty, color and flavor. On the other hand, several species have active biologically substances, which play an important role in health maintenance. These highly perishable food should be grown without the use of pesticides. Thus, several methods are applied to increase the shelf life of food products, as well as ensure their quality and safety. Among the treatments, the food irradiation process has proven to be an effective tool in preserving and extending the shelf life of perishable product. Dianthus chinensis flowers, popularly known as Chinese pink (cravina), belongs to the family Caryophyllaceae, are native to Asia and Europe, are widely used in culinary preparations, being also acknowledged for their bioactive components and antioxidant properties. The purpose of this study was to evaluate carotenoids in D. chinensis flowers submitted to gamma irradiation and electron beam doses of 0.5, 0.8 and 1.0 kGy. High performance liquid chromatography was used to carotenoids determination. In the specie of edible flowers analyzed it was found carotenoid lutein (2.77 to x 7.52 mg/ 100 g). In general, the lutein was higher for irradiated samples, especially those treated with 0.8 and 1.0 kGy independently of irradiation technology. Accordingly, the applied irradiation treatments seemed to represent a feasible technology to preserve the quality of edible flower petals.

Palavras-Chave: carotenoids; electron beams; flowers; gamma radiation; high-performance liquid chromatography; irradiation; radiation dose units; statistical data

KOIKE, AMANDA C.R.; ARAUJO, ELIAS S.; ALMEIDA-MURADIAN, LIGIA B.; VILLAVICENCIO, ANNA L.C.H. Analysis of carotenoids in edible flowers of Dianthus chinensis processed by ionizing radiation. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 21-25, 2019, Santos, SP. Proceedings... Rio de Janeiro: Associação Brasileira de Energia Nuclear, 2019. p. 2007-2014. Disponível em: http://repositorio.ipen.br/handle/123456789/30598. Acesso em: $DATA.

Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.

Como referenciar este item

Abstract: Tetanus is a neurological disease which blocks the inhibitory neurotransmitters liberation. Also treatment does not cure the disease, and its main form of prevention is through vaccination. The vaccine is produced by the inactivation of tetanic toxin (TeNT) with formaldehyde, which may cause side effects. An alternative way is the use of ionizing radiation for inactivation of the toxin and also to improve the potential immunogenic response and to reduce the pos-vaccination side effects. Therefore, the aim of this study was to characterize the TeNT structure after different doses of ionizing radiation of Cobalt 60, and also to assess the enzymatic activity after the radiation. SDS PAGE and MALDI-TOF analysis revealed gradual modification on the TeNT structure according to doses increase. Also, fragmentation and possible aggregations of the protein fragments were observed in higher doses. In the analysis of peptide preservation by enzymatic digestion and mass spectrometry, there was a slight modification in the recognition up to the dose of 4 kGy at subsequent doses, recognition was minimal. The analysis of the enzymatic activity by fluorescence showed a 35 % attenuation in the activity even at higher doses. In the antigenic evaluation performed by ELISA and Western Blot, anti-TeNT antibodies were detected against the irradiated toxins at the different doses, with a gradual decrease as the dose increased, but remaining at satisfactory levels, indicating the possibility of possible use as an immunogen, however, studies of enzymatic activity on higher should be further analyzed.

Palavras-Chave: cobalt 60; detoxification; enzyme activity; gamma radiation; irradiation; neurology; radiation dose units; tetanus; toxins; vaccines

SARTORI, GISELLE P.; COSTA, ANDREA; MACARINI, FERNANDA L.S.; MARIANO, DOUGLAS O.C.; PIMENTA, DANIEL C.; SPENCER, PATRICK J.; GALISTEO JUNIOR, ANDRES J. Characterization and evaluation of the enzymatic activity of tetanus toxin submitted to gamma radiation by Cobalt 60. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 21-25, 2019, Santos, SP. Proceedings... Rio de Janeiro: Associação Brasileira de Energia Nuclear, 2019. p. 2191-2203. Disponível em: http://repositorio.ipen.br/handle/123456789/30616. Acesso em: $DATA.

Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.

Como referenciar este item

Abstract: Conservation and preservation strategies are essential to manage botanical collections specially for dried herbarium specimens also known as exsiccates, usually referring to a set of identified specimens belonging to taxa and distributed among all herbaria around the world. Particularly, these collections are very sensitive to the attack of fungi and insects. In recent years, disinfection by ionizing radiation has become an effective strategy to preserve cultural heritage objects and archived materials with excellent results. In this work, the effects on color properties of gamma radiation on exsiccates samples were studied. Thus, six exsiccates, botanical pressed and dehydrated samples were selected from the Dom Bento José Pickel Herbarium (SPSF), situated at São Paulo (Brazil). Three of these samples comes from Asteraceae family and were collected in 1946, 1984 and 1986, while three other samples belong to Solanaceae family and were collected in 1953, 1984 and 2007. Families of selected botanical collections are very susceptible to biodegradation. The irradiation was performed at the Multipurpose Gamma Irradiation Facility at IPEN applying absorbed doses of 1 kGy, 6 kGy and 10 kGy, which are values of absorbed dose for disinfestation and disinfection. Results were analyzed using colorimetry with CIELAB color space scale and scanning electron microscopy. The results showed that there were no significant changes on colorimetric morphological properties of the samples.

Palavras-Chave: absorbed radiation doses; botany; calorimetry; cobalt 60; disinfestation; fungi; gamma radiation; morphological changes; pest control; plants; radiation dose units; scanning electron microscopy

LIMA, LENI M.P.R.; KODAMA, YASKO; OTUBO, LARISSA; SANTOS, PAULO de S.; VASQUEZ, PABLO A. Effect of ionizing radiation on the color of botanical collections: exsiccata. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 21-25, 2019, Santos, SP. Proceedings... Rio de Janeiro: Associação Brasileira de Energia Nuclear, 2019. p. 2650-2661. Disponível em: http://repositorio.ipen.br/handle/123456789/30649. Acesso em: $DATA.

Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.

Como referenciar este item

Abstract: The development of starch films containing natural antioxidants is one alternative of active packaging. Starch is a well studied natural biopolymer that can be used for the development of biodegradable films because it presents a low cost, is easy to obtain and presents good ability to form films. Hibiscus sabdariffa, commonly known as roselle or red sorrel, is an annual herbaceous sub shrub that contains many types of biocompounds, including organic and phenolic acids. The aim of the present work was to determine the influence of electron beam irradiation on potato starch film containing hibiscus extract. The aqueous hibiscus solution was prepared by boiling for 3 min 1% w/ml dehydrated hibiscus flowers in 500 ml deionized water. The film forming solution was prepared by casting (5% potato starch, 3% glycerol as plasticizer and the hibiscus solution) and irradiated in a 1.5 MeV electron beam accelerator Dynamitron II (Radiation Dynamics Inc.), with doses of 0, 20, 40 and 60 kGy. After drying some mechanical properties were measured. The tensile strength of the control films and the irradiated ones was established. There were no significant differences among them. Hibiscus antioxidants were able to prevent the starch radiation degradation process caused by radiation induced free radicals.

Palavras-Chave: antioxidants; aqueous solutions; electron beams; irradiation; plants; potatoes; radiation dose units; radicals; starch

TEIXEIRA, BRUNA S.; CHIERENTIN, GABRIEL S.; DEL MASTRO, NELIDA L. Impact of electron beam irradiation in potato starch films containing hibiscus aqueous extract. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 21-25, 2019, Santos, SP. Proceedings... Rio de Janeiro: Associação Brasileira de Energia Nuclear, 2019. p. 2114-2122. Disponível em: http://repositorio.ipen.br/handle/123456789/30609. Acesso em: $DATA.

Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.

Como referenciar este item

Abstract: In the present work, the activity concentrations of natural radionuclides 226Ra, 232Th and 40K were analyzed in 32 samples of wheat, corn, manioc and rice flours commonly used by residents of the city of São Paulo, Brazil. All samples were dried, homogenized and tightly sealed in standard 100 mL HDPE flasks with plan screw cap and bubble spigot and measured for 150 ks by gamma spectrometry with an Extended Range Coaxial germanium detector (Canberra GX4020 detector), after approximately 4 weeks storage, in order to ensure secular equilibrium. All spectra were analyzed with the InterWinner 6.0 software. The 226Ra activity concentration was determined from the weighted average concentrations of 214Pb and 214Bi. The 232Th activity concentration was determined from the weighted average concentrations of 228Ac, 212Pb and 212Bi and the 40K one from its single gamma transition. The 226Ra, 232Th and 40K activity concentrations results ranged, respectively, from 0.97 Bq/kg ± 0.42 Bq/kg to 4.75 Bq/kg ± 0.82 Bq/kg, from 8.07 Bq/kg ± 2.36 Bq/kg to 19.02 Bq/kg ± 3.56 Bq/kg and from 25.22 Bq/kg ± 4.38 Bq/kg to 153.5 Bq/kg ± 17.02 Bq/kg.

Palavras-Chave: activity levels; brazil; flour; food; gamma spectroscopy; ge semiconductor detectors; natural radioactivity; radiation dose units; radioactivity; radioisotopes

SILVA, GRAZIELA M. da; PECEQUILO, BRIGITTE R.S. Preliminary results of natural radioactivity in flours commercialized in the city São Paulo by high resolution gamma ray spectrometry. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 21-25, 2019, Santos, SP. Proceedings... Rio de Janeiro: Associação Brasileira de Energia Nuclear, 2019. p. 799-805. Disponível em: http://repositorio.ipen.br/handle/123456789/30525. Acesso em: $DATA.

Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.

Como referenciar este item

Abstract: Worldwide the loss of stored grain is a problem of economic order of importance, in view of the concern of the increased supply of food for a world population increasingly expanding. Associated with this fact, there is the problem of nutritional deficiency due to lack of protein, especially for the less privileged populations in the resources of a country. This lack could be met by adequate supply of grain produced, requiring for it, a system that provides optimum grain storage conditions in securing the quality until the time of consumption. The use of radiation in stored grain can solve the problem of the losses in these products, as it does not induce resistance of insects and leaves no toxic residue to the consumer, and is considered an effective and safe method. The aim of the experiment was to determine the sterilizing dose of ionizing radiation from cobalt-60 to Alphitobius diaperinus (Panzer, 1797)(Coleoptera: Tenebrionidae) in peanuts irradiated in pupa stage. The experiment was conducted in the laboratory of Radiobiology and Environment of the Center for Nuclear Energy in Agriculture - CENA / USP., Piracicaba, SP, Brazil. Peanuts samples infested with pupae of A. diaperinus were utilized in the experiment that consisted of 8 treatments with 5 repetitions. Each repetition consisted of 20 pupae a total of 100 individuals per treatment. Were irradiated with doses of 0 (control), 25, 50, 75, 100, 125, 150 and 175 Gy, in a source of cobalt-60, Gammacell-220 type, with a rate dose of 381Gy / h. The experiment was conducted in a room with temperatures of 25 ± 5 ° C and relative humidity of 70 ± 5%. After of irradiation process was evaluated of the number of emerged adult insects in each repetition in the treatments. The results show that the sterilizing dose in F1 generation was 125 Gy and the pupa lethal dose 150 Gy. The dose of 150 Gy of gamma radiation can be used as phytosanitary treatment to control of immature stages of A. diaperinus infested peanuts.

Palavras-Chave: beetles; cobalt 60; dose rates; insects; irradiation; peanuts; pupae; radiation dose units; radiation doses; sterilization

ARTHUR, PAULA B.; VILLAVICENCIO, ANNA L.C.H.; MACHI, ANDRE R.; LEANDRO, RODRIGO S.R.; ARTHUR, VALTER. Sterilizing of Alphitobius diaperinus (Panzer, 1797) (Coleoptera: Tenebrionidae) irradiated in pupa stage. In: INTERNATIONAL NUCLEAR ATLANTIC CONFERENCE, October 21-25, 2019, Santos, SP. Proceedings... Rio de Janeiro: Associação Brasileira de Energia Nuclear, 2019. p. 2123-2128. Disponível em: http://repositorio.ipen.br/handle/123456789/30610. Acesso em: $DATA.

Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.

Como referenciar este item

Abstract: Nowadays, car tires are not provided with inner air or tubeless, pointing toward a technical evolution. Nevertheless, trucks tires even use at present inner tires, composed almost fully by a synthetic material, which ensures either a good potential for air constraint or longer periods for inspection of tires pressure. Inner tire is located inside the tire, which does not have any extra sealing in the wheel to withstand compressed air. It is designed to resist to expansion of these elements, inside common tires. This rubbery and vulcanized coating has chemical and physical characteristics which enable it to bear a very high air pressurization, avoiding leakages while protects tire outer frame. Inner tires models are exposed to higher temperatures and pressures that contribute to accelerate abrasion. This work aims to the study of mechanical properties changes of an inner tire used in trucks, after gamma rays exposure, in order to promote further material recycling. Ionizing radiation choice was due to its capacity to modify materials structure and properties besides its applicability for rubbers recycling/recovery. For samples characterization, non-irradiated and irradiated at 5, 10, 15, 20, 25 and 30 kGy, there were accomplished following tests: tensile and elongation at break, hardness, and thermal ageing and CHN elementary analysis. It was observed a decrease in mechanical properties for irradiated samples at doses higher than 20 kGy.

Palavras-Chave: chemical properties; cobalt 60; dose rates; gamma radiation; irradiation; mechanical properties; radiation dose units; rubbers; tires; trucks; vulcanization

SCAGLIUSI, SANDRA R.; CARDOSO, ELIZABETH L.C.; ESPER, FABIO J.; LUGAO, ADEMAR B.; WIEBECK, HELIO. Study of mechanical and chemical properties stability of inner tubes exposed to gamma radiation. In: CONGRESSO BRASILEIRO DE POLÍMEROS, 16., 24-28 de outubro, 2021, Ouro Preto, MG. Anais... São Carlos, SP: Aptor Software, 2021. p. 1535-1539. Disponível em: http://repositorio.ipen.br/handle/123456789/32858. Acesso em: $DATA.

Esta referência é gerada automaticamente de acordo com as normas do estilo IPEN/SP (ABNT NBR 6023) e recomenda-se uma verificação final e ajustes caso necessário.

Como referenciar este item