Eventos - Resumos: Recent submissions

  • IPEN-DOC 30011

    MAZER, A.C. ; YORIYAZ, H. . Quantificação da movimentação de órgãos em imagens 4D através de pontos identificados manualmente e automaticamente. In: CONGRESSO BRASILEIRO DE FISICA MEDICA, 27., 12-15 de junho, 2023, São Pedro, SP. Resumo... São Paulo, SP: Associação Brasileira de Física Médica - ABFM, 2023. p. 83-83.

    Abstract: Introdução: A utilização da Radioterapia Guiada por Imagem (IGRT) proporciona um tratamento mais preciso, sendo de extrema importância quantificar a movimentação em órgãos como o pulmão. O objetivo deste trabalho é quantificar a movimentação em uma imagem de Tomografia Computadorizada (TC) 4D através de pontos de referência. Materiais e Métodos: Foram utilizados pontos de referência identificados manualmente, os quais acompanhavam a TC, e pontos identificados automaticamente através de um algoritmo desenvolvido, baseado na técnica SIFT (Scale Invariant Feature Transform). Resultados e Discussões: Obteve-se um valor médio de movimentação de 7,67 ± 5,05 mm e 5,81 ± 3,92 mm entre as fases 0-50 e 50-90, com 100 pontos correspondentes manuais, e 2,84 ± 2,83 mm e 2,63 ± 2,56 mm com 279 pontos extraídos automaticamente. Conclusões: A quantificação da movimentação através da extração de características via algoritmo garante maior precisão a nível do tamanho do voxel das imagens, além de proporcionar a avaliação em outras regiões de interesse da imagem.

    Como referenciar este item
    Icon

  • IPEN-DOC 30010

    JUNOT, D.O.; SOUZA, D.N.; CALDAS, L.V.E. . Viabilidade dosimétrica de compósitos luminescentes à base de CaSO4:Tm,Li. In: CONGRESSO BRASILEIRO DE FISICA MEDICA, 27., 12-15 de junho, 2023, São Pedro, SP. Resumo... São Paulo, SP: Associação Brasileira de Física Médica - ABFM, 2023. p. 59-59.

    Abstract: Introdução: O objetivo desse trabalho foi analisar a viabilidade dosimétrica de compósitos de CaSO4:Tm,Li em dosimetria de feixes de radiação beta, utilizando-se técnicas luminescentes como a termoluminescência (TL) e a luminescência opticamente estimulada (OSL). Materiais e Métodos: A caracterização dosimétrica dos cristais foi realizada utilizandose a Leitora TL/OSL Risø, modelo DA-20. Em todas as análises foi utilizado o filtro Hoya U-340, com banda de transmissão entre 250 nm e 390 nm. As amostras foram irradiadas com fonte beta de 90Sr/90Y, recebendo doses no intervalo de 100 mGy a 50 Gy. Os sinais OSL foram obtidas utilizando-se LEDs azuis, com potência óptica de 90%, durante 40 s. Resultados e Discussões: Os compósitos de CaSO4:Tm,Li apresentaram sinais TL entre 100 ºC e 350 ºC, com picos em torno de 165 ºC e 275 °C. A adição de lítio resultou num aumento significativo do sinal TL total das amostras, visto que a intensidade do pico em torno de 275 °C (temperatura ideal para o pico dosimétrico) praticamente dobrou. O sinal OSL das amostras apresentou-se bastante intenso, cerca de 80x maior que o sinal do CaSO4:Tm, decaindo lentamente e permanecendo armazenado por bastante tempo. Os espectros de emissão TL denotaram emissões típicas dos íons Tm3+ (455 nm). Os coeficientes de variação de homogeneidade e reprodutibilidade ficaram abaixo de 9,5 % e os compósitos apresentaram resposta linear na faixa de dose empregada. Conclusões: O aumento da intensidade luminescente nas amostras de CaSO4:Tm,Li configura uma importante vantagem da adição de lítio nessas amostras, pois resulta em dosímetros mais sensíveis, capazes de detectar doses menores. Tal vantagem possibilita uma ótima aplicação em controle de qualidade, monitoração pessoal e ambiental.

    Como referenciar este item
    Icon

  • IPEN-DOC 30009

    GRANJA, A.A.C. ; BRANCO, I.S.L. ; SHORTO, J.M.B. ; YORIYAZ, H. ; MORAIS, M.C.. Estudo comparativo entre simulações dos códigos de Monte Carlo FLUKA e TOPAS na distribuição da dose longitudinal de feixe de prótons. In: CONGRESSO BRASILEIRO DE FISICA MEDICA, 27., 12-15 de junho, 2023, São Pedro, SP. Resumo... São Paulo, SP: Associação Brasileira de Física Médica - ABFM, 2023. p. 192-192.

    Abstract: Introdução: A Protonterapia se destaca pela entrega pontual da dose, e o Método de Monte Carlo é uma ferramenta eficaz para estudos da área. Este trabalho investigou a distribuição da dose longitudinal de feixes de prótons usando o código FLUKA/Flair, por meio da comparação aos resultados de Branco et al., 2019 com o código GEANT4 /Topas. Materiais e Métodos: Feixes de prótons monoenergéticos de 70, 110, 160, 200 e 230 MeV foram utilizados para analisar a distribuição de dose e o alcance de 90% da dose máxima (d90) em água e cinco materiais distintos, divididos em três grupos (original, densidade da água e composição da água). Resultados e Discussões: Pela Figura 1, nota-se que o alcance d90 para os tecidos originais entre os códigos foi semelhante, e a diferença relativa entre eles permaneceu abaixo de 1,5% para os demais materiais e grupos. Ambos também indicaram maior influência da densidade na dose. Conclusões: Analisando as curvas de deposição de dose e a diferença relativa de d90, pode-se concluir que os códigos apresentaram resultados semelhantes em relação ao transporte de prótons.

    Como referenciar este item
    Icon

  • IPEN-DOC 30008

    FERREIRA, H.J. ; SOUZA, C.D. ; ROSTELATO, M.E.C.M. . Radioactive seed localization for conservative surgery of nonpalpable breast cancer: recommendations for technology implantation program. In: CONGRESSO BRASILEIRO DE FISICA MEDICA, 27., 12-15 de junho, 2023, São Pedro, SP. Resumo... São Paulo, SP: Associação Brasileira de Física Médica - ABFM, 2023. p. 48-48.

    Abstract: Background: The radioactive seed localization (RSL) is used in impalpable breast cancer conservative surgery to assist the surgeon in accurately locating and excising the lesion site. This study aims to present recommendations about the RSL program implementation in health institutions. Methods: An extensive literature review was performed. It comprehends: the committee responsible for implementation of the program actions; description of the necessary multidisciplinary team; the radiological safety committee role; the facility licensing; professionals training; material and instrumentation associated with the technique; and seed tracking system. Results: The Program Implementation Committee must be formed by leaders from each department. The committee assumes responsibility for evaluating the necessary processes and presenting the schedule for program implementation. Since the procedure is classified as a nuclear medicine procedure it requires licensing. The Professional Team Formation, Education, and Training is a priority and simulation exercises are necessary. The Materials and Instrumentation Associated with the Technique must be well-know by the team and they should practice using radiation detectors. The seed must be always tracked, from moment they are received to discard. An Inventory for Tracking Seeds is provided. The Radiological Safety Aspects such as the ALARA principle are presented. A full description for the Radiological Procedure for Placing the seeds, the surgical removal and the Specimen Handling in Pathology focusing on how to locate the seed and retrieve them. After removed, the seeds can be placed in storage to wait for full radioactive decay or be returned to the manufacturer. Conclusions: Regular multidisciplinary team meetings during program development are important to create a realistic timeline, having briefing meetings after the first 1-5 RSL cases and having annual or biannual follow-up meetings to discuss any issues or incidents.

    Como referenciar este item
    Icon

  • IPEN-DOC 30007

    EMILIOZZI, C.Z.S.; MENEZES, M.O. . Estudo do tempo de espera para tratamentos de Radioterapia. In: CONGRESSO BRASILEIRO DE FISICA MEDICA, 27., 12-15 de junho, 2023, São Pedro, SP. Resumo... São Paulo, SP: Associação Brasileira de Física Médica - ABFM, 2023. p. 194-194.

    Abstract: Introdução: Os sistemas de saúde têm dado maior atenção para o feedback do paciente sobre a qualidade do serviço recebido e sua satisfação é sempre influenciada pela sua percepção sobre o tempo de espera. Nesse trabalho buscou-se propor tempos de espera de referência para fornecer para os pacientes da instituição. Materiais e Métodos: Na radioterapia do HCFMUSP foi realizado um estudo retrospectivo entre 2016 e 2020 para propor um tempo de espera baseado na estimativa superior do intervalo de confiança de 95% (TES) e na mediana. As características do paciente como grupo de tratamento, faixa etária, se é proveniente de convênio e técnica de tratamento foram selecionadas para a realização do agrupamento do tempo de espera. Resultados e Discussões: A tabela 1 apresenta alguns resultados encontrados. A Técnica VMAT e não convênio têm maiores tempo de espera. O TES é uma estimativa de tempo de espera máximo e a mediana de tempo de espera mais provável. Conclusões: O estudo do tempo de espera ajuda no gerenciamento do fluxo de trabalho e uma previsão de espera mais exata pode influenciar na satisfação do paciente.

    Como referenciar este item
    Icon

  • IPEN-DOC 30006

    DIAS, F.S. ; SANTOS, L.C. ; POTIENS, M.P.A. . Avaliação do desempenho de diferentes câmaras de ionização do tipo lápis em feixes de calibração. In: CONGRESSO BRASILEIRO DE FISICA MEDICA, 27., 12-15 de junho, 2023, São Pedro, SP. Resumo... São Paulo, SP: Associação Brasileira de Física Médica - ABFM, 2023. p. 94-94.

    Abstract: Introdução: A tomografia computadorizada TC é responsável por grande parte da dose em pacientes. Logo, é importante que os equipamentos medidores de radiação sejam calibrados em laboratórios que possuam rastreabilidade metrológica. Para a realização da dosimetria em feixes de TC, é necessária uma câmara de ionização do tipo lápis, além de seguir o protocolo TRS/IAEA (2007). O objetivo deste trabalho é a realização de uma comparação do desempenho de câmaras de ionização do tipo lápis com variados volumes, durante um procedimento de calibração. Materiais e Métodos: O dosímetro referência foi a câmara de ionização modelo RC3CT, de 3 cm3, conectada ao eletrômetro PTW / Unidos T10010, que possibilitou a a determinação da taxa de kerma no ar com rastreabilidade ao laboratório primário PTB. O estudo abrangeu as câmaras de ionização modelo RC3CT e 10X6 – 0,6 conectadas ao Multimedidor RADCAL do sistema Accu-Gold+. A metodologia adotada foi a recomendada pelo código de prática TRS 457 (/IAEA, 2007) utilizando o método da substituição. Resultados e Discussões: A tabela 1 mostra os resultados obtidos para os equipamentos testados em comparação com o sistema de referência em termos de taxa de kerma no ar. A diferença encontrada variou entre 0,12 a 4,2%. Conclusões: Apesar dos valores de taxa de kerma terem ficado próximos, vale ressaltar as dificuldades em se calibrar câmaras com um sistema próprio acoplado, principalmente os que não utilizam eletrômetros na calibração.

    Como referenciar este item
    Icon

  • IPEN-DOC 30005

    BRANCO, I.S.L. ; SIQUEIRA, P.T.D. ; SHORTO, J.M.B. ; YORIYAZ, H. . Análise comparativa de modelos matemáticos no desenvolvimento de um Algoritmo Pencil Beam para protonterapia. In: CONGRESSO BRASILEIRO DE FISICA MEDICA, 27., 12-15 de junho, 2023, São Pedro, SP. Resumo... São Paulo, SP: Associação Brasileira de Física Médica - ABFM, 2023. p. 168-168.

    Abstract: Introdução: Em protonterapia, a distribuição de dose é calculada por meio de algoritmos analíticos, como o Algoritmo Pencil Beam (PBA), que requer a estimativa de parâmetros para a fluência no ar e dose na água. Este estudo utilizou o código TOPAS para modelar o feixe e analisou a influência dos modelos matemáticos no cálculo de dose com o PBA. Materiais e Métodos: Simulações com o TOPAS calcularam a fluência no ar, dose elementar na água e dose de referência para prótons de 187 MeV. Três distintos modelos matemáticos parametrizaram a distribuição de fluência no ar e cinco a dose elementar na água, resultando em 15 convoluções que foram comparadas com a dose de referência. A Figura 1 apresenta as diferenças relativas de dois dos resultados obtidos. Resultados e Discussões: Entre as convoluções realizadas, destaca-se a que utilizou a fluência ajustada com a Gaussiana Dupla e a dose elementar com a Gaussiana Dupla e Cauchy. Essa convolução apresentou pequenas diferenças relativas nas doses laterais de entrada do feixe e menores diferenças quando comparadas aos outros modelos. Conclusões: A precisão dos cálculos de dose no PBA é grandemente influenciada pela escolha dos modelos matemáticos para parametrizar a fluência e dose elementar, especialmente para baixas doses (distantes do eixo central).

    Como referenciar este item
    Icon

  • IPEN-DOC 30004

    ALMEIDA JUNIOR, J.N. ; POTIENS, M.P.A. ; RODRIGUES JUNIOR, O. . Desenvolvimento de um protótipo semiautomatizado para posicionamento de filtros para calibração em sistemas de radiação X. In: CONGRESSO BRASILEIRO DE FISICA MEDICA, 27., 12-15 de junho, 2023, São Pedro, SP. Resumo... São Paulo, SP: Associação Brasileira de Física Médica - ABFM, 2023. p. 141-141.

    Abstract: Introdução: Para calibração de equipamentos utilizados em sistemas de radiação X (radioproteção, radiodiagnóstico e radioterapia) é necessário um sistema adequado de posicionamento de filtros de radiação que compõem uma qualidade de radiação de referência. Com objetivo de aperfeiçoar o setup utilizado nos procedimentos de calibração de instrumentos medidores de radiação utilizados em processos de radioterapia, radioproteção e radiodiagnóstico no Laboratório de Calibração de Instrumentos do Instituto de Pesquisas Energéticas Nucleares (IPEN-CNEN), se faz necessário desenvolvimento de sistema confiável de posicionamento da respectiva filtração. O projeto visa adicionar um novo sistema de filtração para aumentar a capacidade existente da roda de filtros e adaptá-la a novos arranjos. Materiais e Métodos: Foi desenvolvido um sistema gaveta/porta-filtro em filamento PLA com impressão 3D, ajustando parâmetros para melhor precisão. A identificação do filtro é realizada com micro chaves e microprocessador Arduino, integrando ao sistema de controle da roda de filtro do laboratório. Com o sistema gaveta/porta-filtro, cria-se uma metodologia para calibrar medidores de produto kerma-área (PKA), identificando filtros e caracterizando feixes de radiação. Resultados e Discussões: O sistema pode ser adaptado para outros arranjos de filtros e será usado para medir o (PKA) instrumento de referência, considerando as características de cada equipamento de raios-X. Conclusões: O sistema porta-filtros será utilizado para calibrar medidores de PKA e PDC em equipamentos de radiologia, e com os arranjos experimentais do laboratório sendo simulados utilizando o código TOPAS, pretendemos validar o sistema por meio do Método de Monte Carlo, para avaliar grandezas e a dependência energética.

    Como referenciar este item
    Icon

  • IPEN-DOC 30003

    ARAUJO, M.S. ; COSTA E SILVA, D.L. ; IZIDORO, J. ; MELLO-CASTANHO, S. ; FUNGARO, D.A. . Remoção de césio-137 em efluentes por adsorção em zeólita A sintetizadas a partir das cinzas do bagaço de cana-de-açúcar. In: CONGRESSO BRASILEIRO DE CERÂMICA, 67., 12-15 de junho, 2023, Florianópolis, SC. Resumo... 2023.

    Abstract: Em decorrência do uso crescente de tecnologias com atividade nuclear, está a geração de rejeitos contaminados, em particular, os efluentes os quais representam grandes volumes de água contendo radionuclídeos ainda ativos em dispersão. Dentre os radionuclídeos gerados, o césio-137 (137Cs) está entre os mais preocupantes pelo fato de ser um emissor de raios gama e, ser também, um dos isótopos mais abundantes nos rejeitos radioativos da atividade nuclear e possuir meia-vida relativamente longa (30,4 anos). Atualmente, a adsorção é um dos métodos mais atraentes para a retenção de 137Cs em termos do bom desempenho de remoção de maneira segura. Vários materiais adsorventes inorgânicos têm sido amplamente utilizados, tendo destaque as zeólitas, que devido ao seu alto grau de cristalinidade, garantem maior seletividade e eficiência na adsorção em comparação a outros materiais como alumina, sílica gel e carvão ativo. Ainda, a possibilidade de ser sintetizada a partir de resíduos como as cinzas de bagaço de cana-de-açúcar, a torna um material de baixo custo. Desta forma, a presente pesquisa foi conduzida por meio do estudo da adsorção de uma solução de cloreto de césio por zeólita A sintetizada a partir das cinzas do bagaço de cana-de-açúcar e do impacto dos íons de Cs+ adsorvidos em sua estrutura cristalina. As amostras foram caracterizadas por diferentes técnicas a fim de avaliar sua composição química, tamanho de partícula, estrutura cristalina e comportamento térmico. Os resultados indicam a obtenção de zeólita tipo NaA de alta pureza e com elevada capacidade de adsorção. Ainda, o teor de sódio presente na zeólita foi completamente substituído pela troca iônica de íons de Na+ por íons de Cs+. Portanto, o material sintetizado é uma solução promissora para o tratamento e remoção de césio-137 em efluentes gerados contaminados.

    Como referenciar este item
    Icon

  • IPEN-DOC 29999

    SILVA, CAMILA R. ; RIBEIRO, MARTHA S. . Terapia fotodinâmica associada à quimioterapia em células de câncer de mama triplo-negativo. In: RIBEIRO, MARTHA S. (Ed.) FORUM ON-LINE DE TECNOLOGIAS DA LUZ NA SAUDE, 3., 26-28 de maio, 2022, São Paulo, SP. Resumo... 2022. p. 25-25.

    Abstract: INTRODUÇÃO: O câncer de mama triplo-negativo (TNBC) representa 20% das incidências de câncer de mama com uma mortalidade estimada em 40%. As principais características desse tipo de câncer seriam o seu alto poder de metástase e resistência aos tratamentos convencionais como a quimioterapia. Diante disso, há uma crescente busca por terapias adjuvantes para auxiliar o tratamento do TNBC. Nesse contexto, a terapia fotodinâmica (TFD) têm se mostrado promissora no tratamento de diversos tipos de câncer e recentemente a literatura descreve o seu uso em associação com os tratamentos convencionais para auxiliar no combate ao câncer. OBJETIVO: Associar a TFD com quimioterapia no tratamento de células TNBC. METODOLOGIA: Células TNBC, células de câncer de mama responsivas à quimioterapia (MCF-7) e células não tumorais de mama (MCF-10A) foram cultivadas, semeadas (2 x 104 células) e após a TFD usando a porfirina TMPyP (30 μM) e luz vermelha (λ= 660 ± 11 nm) com as fluências de 20 J/cm² e 50 J/cm² receberam doxorrubicina (DOX) na concentração de 5 μg/mL. Após 24-h da quimioterapia, a atividade mitocondrial foi avaliada para todos os grupos experimentais (n=6). RESULTADOS: As células MCF-7 e MCF-10A usadas como controle apresentaram redução significativa na atividade mitocondrial quando expostas à DOX. Por outro lado, as células TNBC não apresentaram redução estatisticamente significativa quando submetidas ao mesmo protocolo. No entanto, na associação da TFD+DOX, as células TNBC apresentaram redução significativa na atividade mitocondrial, independente da fluência usada. CONCLUSÃO: A associação da TFD com doxorrubicina apresentou menor atividade mitocondrial para as células TNBC.

    Como referenciar este item
    Icon

  • IPEN-DOC 29998

    SILVA, ABDENEGO R. ; SANTOS, BEATE S.; REBOUCAS, JULIO S.; FONTES, ADRIANA; RIBEIRO, MARTHA S. . Estratégia antifúngica baseada em luz: uma abordagem para controle de infecção disseminada por Candida auris. In: RIBEIRO, MARTHA S. (Ed.) FORUM ON-LINE DE TECNOLOGIAS DA LUZ NA SAUDE, 3., 26-28 de maio, 2022, São Paulo, SP. Resumo... 2022. p. 26-26.

    Abstract: INTRODUÇÃO: Infecções fúngicas por Candida auris são, atualmente, um problema grave e emergente, devido à sua alta resistência aos antifúngicos convencionais. A terapia fotodinâmica (PDT, do inglês PhotoDynamic Therapy) vem se consolidando como uma opção de tratamento não invasiva, demostrando bons resultados na erradicação de microrganismos. Em virtude do seu mecanismo de ação, não há evidências na literatura que a PDT promova resistência microbiana. Além disso, esta terapia é igualmente efetiva na inativação de microrganismos resistentes aos antimicrobianos convencionais. OBJETIVO: Desenvolver um modelo de infecção disseminada em animais imunossuprimidos infectados por C. auris, bem como avaliar o potencial antifúngico da PDT mediada por azul de metileno (AM) e por uma zincoporfirina (ZnHexP) no respectivo modelo. METODOLOGIA: Em um primeiro momento, os animais [camundongos C57BL/6 (n: 8/grupo)] serão avaliados quanto ao estado leucopênico através de irradiação de corpo inteiro por raios-γ e, após constatação, a infecção disseminada será induzida inoculando C. auris por gavagem e a sobrevida dos animais será avaliada. Após estabelecimento do modelo, os animais serão submetidos à PDT mediada por 2 diferentes fotossensibilizadores (FSs): AM e ZnHexP em duas diferentes concentrações. Os FSs serão administrados pela veia caudal e, após 24 h, os animais receberão irradiação de corpo inteiro com luz vermelha e azul, respectivamente. Os animais serão monitorados clinicamente até eutanásia. Serão coletadas amostras de sangue, urina, fezes, fígado e rins para quantificação da carga fúngica. Serão utilizados 12 grupos experimentais entre tratados e controles. RESULTADOS ESPERADOS: Espera-se que este estudo colabore para o avanço da PDT no controle de infecções fúngicas, principalmente de uma infecção disseminada. Também espera-se identificar a melhor combinação de luz e FS para este tratamento.

    Como referenciar este item
    Icon

  • IPEN-DOC 29997

    MENDES, VALERIA ; AZEVEDO, LUCIANE H.; ZEZELL, DENISE M. . Lasers não-ablativos de alta intensidade na apneia obstrutiva do sono. In: RIBEIRO, MARTHA S. (Ed.) FORUM ON-LINE DE TECNOLOGIAS DA LUZ NA SAUDE, 3., 26-28 de maio, 2022, São Paulo, SP. Resumo... 2022. p. 3-3.

    Abstract: INTRODUÇÃO: O ronco primário e apneia grave representam as diferentes gravidades do mesmo distúrbio fisiopatológico, que afeta em torno de um bilhão de pessoas em todo o mundo. É mais frequentemente em homens e idosos. O ruído é causado pela vibração dos tecidos moles na área mais constrita das vias aéreas superiores. As opções terapêuticas usuais apresentam limitações e as publicações em literatura sobre os efeitos clínicos do uso de laser no manejo do ronco e AOS são escassas e pouco exploradas. OBJETIVOS: Após determinar as melhores condições de irradiação, este estudo tem como objetivo, avaliar o tratamento do ronco com irradiação de alta intensidade, sequencial e não-ablativa de dois lasers pulsados: Nd:YAG e Er:YAG (Lightwalker, Fotona). Os resultados obtidos com o tratamento são comparados às condições em linha de base. METODOLOGIA: Um estudo longitudinal, intervencionista e prospectivo está em andamento com a aprovação do comitê de ética em pesquisa. Até o momento, 32 de 40 voluntários que transitam entre ronco primário e apneia obstrutiva do sono moderada foram tratados em três sessões de irradiação laser não-ablativa. O intervalo entre as sessões foi de14 dias. O volume do espaço orofaríngeo foi analisado por registro fotográfico, de acordo com o índice Mallampati modificado. Saturação de oxigênio, gravidade do ronco e qualidade do sono também serão avaliados. RESULTADOS ESPERADOS: O principal resultado clínico obtido será a expansão da luz da via aérea superior pela diminuição da complacência tecidual. Com isso, redução ou eliminação do ronco.

    Como referenciar este item
    Icon

  • IPEN-DOC 29996

    FERREIRA, DENISE R. ; NASCIMENTO, MAURICIO M. do; BAGATIN, EDILEIA; CORTEZ, GABRIEL; RIBEIRO, MARTHA S. . Viabilidade, segurança e eficácia da terapia de fotobiomodulação versus minoxidil 5% tópico no tratamento da alopecia androgenética feminina: relato de caso. In: RIBEIRO, MARTHA S. (Ed.) FORUM ON-LINE DE TECNOLOGIAS DA LUZ NA SAUDE, 3., 26-28 de maio, 2022, São Paulo, SP. Resumo... 2022. p. 30-30.

    Abstract: INTRODUÇÃO: A alopecia androgenética feminina (AAGF) é a principal causa de queda de cabelo nas mulheres. Caracteriza-se pela rarefação dos cabelos na região central e frontal do couro cabeludo. Uma vez que o cabelo é considerado um atributo de beleza, a sua perda pode causar impacto negativo na qualidade de vida da paciente. O uso do Minoxidil 5% (MNX) em solução tópica é considerado tratamento de escolha, com resultados variáveis. A utilização da terapia de fotobiomodulação (FBM) tem se mostrado uma tecnologia promissora, embora existam poucos relatos na literatura. OBJETIVO: Avaliar a viabilidade, segurança e eficácia da FBM como um adjuvante ao Minoxidil 5% no tratamento da AAGF. METODOLOGIA: Inclusão de 2 mulheres entre 30 a 50 anos com diagnóstico de AAGF de grau 2 a 4 de Sinclair, sem uso de anticoncepcional. Foi realizado fotografia padronizada da linha média do topo da cabeça para acompanhar a evolução do crescimento capilar. As participantes foram então orientadas a aplicar todos os dias, 7 jatos de minoxidil 5% em todo topo da cabeça limpa, 1 vez ao dia e comparecer ao ambulatório 2 vezes por semana para aplicação do laser de 660 nm, em um dos lados randomizados da cabeça. A aplicação foi realizada em modo pontual, numa distância de 0,5 cm e energia de 4J por ponto. RESULTADOS: Após 1 mês de aplicação do laser, foi possível observar através de fotografia padronizada a redução da linha média, assim como aumento da espessura normal dos fios. Além disso, as pacientes relataram que houve menor queda do número dos fios durante esse período. CONCLUSÃO: Os resultados preliminares sugerem que o uso da FBM por 1 mês no manejo de AAGF reduz o número da queda dos fios, sendo necessário finalizar todo protocolo para observar outros parâmetros como redução dos fios miniaturizados através do fototricograma.

    Como referenciar este item
    Icon

  • IPEN-DOC 29995

    SALANI, R.; DEANA, A.M.; SILVA, D.F.T. ; PAVANI, C.. Optical properties of the antibiotic Levofloxacin. In: MESQUITA-FERRARI, RAQUEL A. (Ed.); HORLIANA, ANNA C.R.T. (Ed.); CECATTO, REBECA B. (Ed.); FERNANDES, KRISTIANNE P.S. (Ed.) BIOPHOTONICS INTERNATIONAL FORUM, 8th, November 8-9, 2022, São Paulo, SP. Abstract... São Paulo, SP: Universidade Nove de Julho - UNINOVE, 2022. p. 57-57.

    Abstract: In an infectious clinical condition, the gold standard treatment is antibiotic therapy. However, some medications could suffer photodegradation when exposed to light (artificial, environmental, or a therapy as photobiomodulation). Light absorption by these molecules could initiate chemical reactions. Thus, an understanding of how light interacts with antibiotic (ATB) is necessary and this is possible through the study of its optical properties. To measure diffuse transmittance (Td) and diffuse reflectance (Rd) of levofloxacin (LVX) and indirectly to determine the absorption (μa), reduced scattering (μ’s) and reduced attenuation (μ’t) coefficients of this ATB. The ATB used was the LVX (500mg, tablet, Tavok®). The tablet was macerated and the powder was solubilized in MilliQ® water at a final concentration of 10x10-3mol/L. A spectrophotometer was used to register the Td and Rd spectra of the solution. Quartz cuvettes with an optical path of 1.0cm were used and the data were registered in the range of 400 to 1000nm. From the Td and Rd spectra, the values of the μa, μ’s and μ’t of the ATB were calculated using the Kubelka-Munk function. Statistical analysis to compare ATB coefficients at 450, 530, 590, 660, 780 and 810nm was performed using the Origin software. Two way-ANOVA with Tukey post hoc test and significance level α<0.05 was used. The Td value was 10% at 450nm and increased to 32% at 810nm. The Rd was 45% at 450nm and decreased to 19% at 810nm. There was no significant difference in μa (0.44 ±0.18cm-1 at 450nm, 0,56±0.27cm-1 at 530nm, 0.64±0.33cm-1 at 590nm, 0.59±0.32cm-1 at 660nm, 0.47± 0.31cm-1 at 780nm and 0.44±0.31cm-1 at 810nm). The μ's decreased with increasing wavelength being 3.08 ± 1.84cm-1 at 450nm, 0.92±0.51cm-1 at 660nm, 0.71±0.42cm-1 at 780nm and 0.65±0.39cm-1 at 810nm. The μ't also decreased with the increasing wavelength being 3.52±1.94cm-1 at 450nm, 1.52±0.82cm-1 at 660nm, 1.18±0.73cm-1 at 780 nm and 1.09±0.70 cm-1 at 810nm. The presence of red and yellow ferric oxide can be related to the high μa values. The low values of Td and high values of the μ’s can be related to the magnesium stearate present in the tablet. The high values of μ’t suggest that ATB absorbed light. The values of the coefficients studied may have been influenced by the excipients present in the tablet formulation. Therefore, future studies with the pure active ingredient of ATB may provide more concrete information about the optical properties of LVX.

    Como referenciar este item
    Icon

  • IPEN-DOC 29994

    FIGUEIREDO, R.F. ; BELOTTO, R.A.; POZZO, L. ; ROSTELATO, M.E. ; RIBEIRO, M.S. . Could light-based technologies replace high potency corticosteroids in the treatment of vulvar lichen sclerosus?. In: MESQUITA-FERRARI, RAQUEL A. (Ed.); HORLIANA, ANNA C.R.T. (Ed.); CECATTO, REBECA B. (Ed.); FERNANDES, KRISTIANNE P.S. (Ed.) BIOPHOTONICS INTERNATIONAL FORUM, 8th, November 8-9, 2022, São Paulo, SP. Abstract... São Paulo, SP: Universidade Nove de Julho - UNINOVE, 2022. p. 7-7.

    Abstract: INTRODUCTION: Vulvar lichen sclerosus (LS) is considered a chronic inflammatory dermatosis that affects woman's anogenital area and may cause scarring of the vulva and sexual dysfunction. Currently, there is no cure for LS and the treatment is based on the topical application of high-potency corticosteroids (HPCs). The cutaneous atrophy, irritation, hypopigmentation, blistering, and dryness that HPCs cause are, however, undesirable side effects. Light-based technologies have been employed as an alternative for LS therapy since they are less invasive and promote positive results. OBJECTIVE: Systematically examine whether light-based technologies may be a suitable option for the treatment of LS. METHODOLOGY: We followed the guidelines recommended by PRISMA to conduct this review and registered the protocol on the PROSPERO. We searched original articles published in English on Medline, Web of Science, Embase, Clinical Trials, and Cochrane databases. We covered all light-based technologies for treating LS using the following strategy: ((LIGHT OR LASER OR LED OR LIGHT-EMITTING DIODE OR PHOTOTHERAPY OR PHOTODYNAMIC) AND (VULVAR LICHEN SCLEROSUS)). For eligibility, studies should present the number of patients per group, consistent methodology, reliable light source parameters, and HPC-treated control group. For the assessment of the quality of included studies, we used the Cochrane risk of bias (RoB) tool. RESULTS: Overall, the search turned up 582 articles. Of these, 579 were excluded, including duplicates (n= 227), reviews, letters, comments, preclinical studies (n= 292) articles without an HPC control group (n = 34), light-based technologies used as an adjuvant (n= 2), ongoing trials (n= 17), and other diseases associated with LS (n= 7). As a result, three studies were included in which 3 different technologies were used: CO2 high power laser, 5-aminolevulinic acid-mediated photodynamic therapy (ALA-PDT), and ultraviolet-A (UVA) phototherapy. UVA caused erythema, pruritus, skin xerosis, and tanning, even though it has promoted less burning and suffering. In contrast, CO2 laser and ALA-PDT were able to provide higher patient satisfaction. Overall, RoB was considered low for the articles assessed. CONCLUSION: Despite the lack of a defined treatment procedure, ALA-PDT and CO2 laser are both promising approaches to treat LS. More randomized clinical trials using light-based technologies should be conducted to improve evidence-based healthcare for women with LS.

    Como referenciar este item
    Icon

  • IPEN-DOC 29993

    PRATES, BRENDA C.R. ; POZZO, LORENA . Avaliação de desempenho da incorporação de equipamentos PET/CT no SUS. In: CONGRESSO BRASILEIRO DE SAUDE COLETIVA, 13., 21-24 de novembro, 2022, Salvador, BA. Resumo... Campinas, SP: Galoá, 2022.

    Abstract: A incorporação de tecnologias de alta complexidade como o PET/CT deve ser constantemente analisada e aprimorada para que ocorra favorecendo sua consolidação. O estudo de avaliação quanto ao desempenho desses equipamentos contribui no entendimento da efetividade clínica, da utilização desses na vida real do paciente e do impacto dessa tecnologia nas diferentes regiões em que estão inseridas. Objetivos Avaliar o desempenho de equipamentos PET/CT incorporados pelo SUS nos domínios admissibilidade, técnico, econômico e inovação que permeiam essa tecnologia, conforme Diretriz Metodológica de Elaboração de Estudos para Avaliação de EMA. Metodologia As metodologias basearam-se na busca ativa de informações a partir de levantamentos bibliográficos e consultas em sites que apresentassem dados de interesse. Consultou-se o site da ANVISA para aquisição dos registros sanitários e manuais de operações, além do banco de dados do CNES e CNEN, para a quantidade de estabelecimentos e instalações radioativas autorizadas. Neste último foi possível obter o número de profissionais devidamente certificados. Valores de equipamentos e financiamento de projetos foram obtidos através do site do FNS, já os valores dos procedimentos foram extraídos junto ao SIGTAP. Requisitos operacionais de SMN foram consultados nas normativas estabelecidas pela CNEN. Resultados Identificou-se treze registros de equipamentos de sete fabricantes distintos com registro sanitário dentro do período de vigência junto à ANVISA. Há 104 inscrições junto ao CNES de estabelecimentos com pelo menos um equipamento PET/CT e 160 matrículas de instalações autorizadas pela CNEN. O valor sugerido para tais equipamentos em 2022, divulgado pelo FNS é R$ 6.405.000,00. São registradas 7 propostas de financiamentos aprovadas, distribuídas em 5 cadastros de estabelecimentos. Os impostos observados são: IPI, PIS e COFINS. O valor pago pela tabela SUS é R$ 2.107,22 por procedimento. Profissionais devidamente certificados pela CNEN estão concentrados principalmente na região sudeste do país. Conclusões/Considerações De maneira geral, percebeu-se dificuldade para se obter alguns dados que permeiam a tecnologia PET/CT. A maneira de disponibilização das informações, visto que estas são autodeclaradas pelas partes interessadas (fabricantes e estabelecimentos), corroboram com isso. Outro fator, é ser tratar da incorporação de uma tecnologia de alta complexidade em plena utilização, sendo observados poucos estudos que se propõem a avaliar seu desempenho.

    Como referenciar este item
    Icon

  • IPEN-DOC 29992

    PAIVA, MARCIA M.D.P. ; POZZO, LORENA . Análise de custo de tratamento para pacientes com diagnóstico de câncer de próstata com recidiva bioquímica submetido ao [68Ga] PSMA - PET-CT. In: CONGRESSO BRASILEIRO DE SAUDE COLETIVA, 13., 21-24 de novembro, 2022, Salvador, BA. Resumo... Campinas, SP: Galoá, 2022.

    Abstract: A Recidiva Bioquímica (RB) do câncer de Próstata (CP) já tratado é identificada através da elevação do Antígeno Prostático Específico (PSA). A tecnologia [68Ga] PSMA PET-CT é um método diagnóstico não invasivo realizado no momento da RB que pode evidenciar a localização da doença e sua extensão para otimização do tratamento. Objetivos O objetivo do presente trabalho foi analisar o custo de tratamento para pacientes com diagnóstico de primeira recidiva bioquímica de câncer de próstata após a realização de [68Ga] PSMA PET-CT sob a perspectiva do Sistema Único de Saúde. Metodologia Levantamento bibliográfico de revisões sistemáticas até o ano de 2021 para elaboração do cenário de restadiamento diagnóstico 1, considerando o exame [68Ga] PSMA PET-CT. Revisão de diretrizes e guidelines para o cenário 2, considerando o Método Padrão de Imagem (Tomografia Computadorizada, Ressonância Magnética e Cintilografia Óssea). Aplicação de formulário (Delphi) a painel de especialistas para criar modelo de tratamento para cada cenário. Neste trabalho, foi usada a Tabela Unificada de Procedimentos do SUS como referencia para levantamento dos custos de cada tratamento. Resultados Cenário de restadiamento diagnóstico 1 com 4 possibilidades: Doença localizada (DL) , Linfonodos pélvicos (LP), Oligometástases e Metástases. Cenário de restadiamento diagnóstico 2: DL e metástases. Conforme painel de especialistas os tratamentos seriam os seguintes: DL: Radioterapia (RT) de resgate, LP: RT de resgate, Terapia de privação androgênica (TDA) ou Tratamento Sistêmico (medicamentos e RT), Oligometástases: Tratamento Sistêmico, Radiocirurgia Estereotáxica Corpórea (SBRT) e TDA, Metástases: Quimioterapia, Terapia Combinada (medicamentos e hormânios) ou TDA. Resultado negativo ou duvidoso: A RT de Resgate ou Pélvica será utilizada considerando os aumentos consecutivos do PSA. Conclusões/Considerações A análise dos custos dos tratamentos para pacientes em primeira recidiva bioquímica demonstram que os gastos são maiores no cenário 1 com custo total de R$ 264.965.465,00, após a realização do [68Ga] PSMA PET-CT e de R$ 123.585.612,72 no cenário 2, após o método de imagem padrão. Ainda são necessários estudos de seguimento prolongado do paciente após alteração de conduta resultante do [68Ga] PSMA PET-CT para verificação de custo-efetividade.

    Como referenciar este item
    Icon

  • IPEN-DOC 29991

    OLIVEIRA, MERCIA L. de; MENEZES, MARIO O. de ; TOCAS, FOTINI; POZZO, LORENA . Alta complexidade no SUS: o exemplo da medicina nuclear. In: CONGRESSO BRASILEIRO DE SAUDE COLETIVA, 13., 21-24 de novembro, 2022, Salvador, BA. Resumo... Campinas, SP: Galoá, 2022.

    Abstract: As demandas regionais diferenciadas, os cenários de limitações orçamentárias, os interesses do capital especulativo, os fatores externos, entre outros, representam grandes desafios para a gestão e funcionamento do Sistema Único de Saúde (SUS) e tendem a limitar o acesso da população aos serviços de saúde. O setor da alta complexidade torna-se emblemático no contexto da discussão sobre o SUS. Objetivos Análise da distribuição de estabelecimentos e equipamentos de medicina nuclear no país: aceleradores de partículas, gama câmaras e tomógrafos por emissão de pósitrons com tomografia computadorizada (PET/CT). Metodologia Os dados relativos ao número de instalações e à localidade foram obtidos na base de dados do Cadastro Cadastro Nacional de Estabelecimentos de Saúde (CNES - DATASUS) (http://cnes2.datasus.gov.br/Mod_Ind_Equipamento.asp), utilizando-se os filtros PET/CT e gama câmara. As informações acerca dos aceleradores de partículas (cíclotrons) foram obtidas no sítio da Comissão Nacional de Energia Nuclear (CNEN) (http://antigo.cnen.gov.br/index.php/instalacoes-autorizadas-2). Os dados se referem ao ano de 2022. Resultados Segundo os dados referentes a 2022, 614 estabelecimentos possuem gama câmara, sendo que 134 estão localizados na cidade de SP. Do total das instalações, 310 instalações recebem pacientes do SUS. Estão cadastradas na CNES/DATASUS 102 instalações com PET/CT, sendo que 56 atendem ao SUS com grande concentração no Estado de SP (27). Não existem instalações PET/CT nos estados do AC, AP, RO, RR, TO, AL e CE. Com relação aos estabelecimentos com aceleradores de partículas, na base de dados na CNEN, estão listadas 10 instalações, sendo três em SP e nenhum na região Norte. Conclusões/Considerações As assimetrias na distribuição de tecnologias para serviços de medicina nuclear no país revelam as desigualdades regionais. A alta concentração e os vazios tecnológicos corroboram com a imensa dificuldade de difusão dos serviços de alta complexidade no SUS. As tecnologias de alta complexidade têm alto potencial de gerar iniquidades exigindo esforços governamentais para reduzir possíveis vazios assistenciais decorrentes de vazios tecnológicos.

    Como referenciar este item
    Icon

  • IPEN-DOC 29803

    GASPARIAN, PATRICIA B.R. ; MALTHEZ, ANNA L.M.C.; BICALHO, DANIEL B.; CAMPO, ALEXANDRE B.; CAMPOS, LETICIA L. . Performance test of a matrix for radiation detector OSL films. In: BRAZIL MRS MEETING, 20th, September 25-29, 2022, Foz do Iguaçu, PR. Abstract... São Carlos, SP: Aptor Software, 2022.

    Como referenciar este item
    Icon

  • IPEN-DOC 29784

    ZAMBONI, CIBELE B. ; METAIRON, SABRINA ; GIOVANNI, DALTON N.S. ; MEDEIROS, JOSE A.G. de ; AZEVEDO, MARIA R. . Ions and metal dosage in human health: trends and progress using radiations. In: REUNIÃO DE TRABALHO SOBRE FÍSICA NUCLEAR NO BRASIL, 45; ENCONTRO NACIONAL DE FÍSICA DE PARTÍCULAS E CAMPOS 42, 26-29 de setembro, Natal, RN. Resumo... 2022.

    Como referenciar este item
    Icon

  • IPEN-DOC 29763

    DELLAMANO, JOSE C. ; DAMATTO, SANDRA R. ; MADUAR, MARCELO F. ; MARUMO, JULIO T. ; CARVALHO, RICARDO N. ; VICENTE, ROBERTO ; MENEGHINI, ARTHUR A. . In situ characterization of NORM waste from the oil industry. In: DANAHER, T. (Org.) INTERNATIONAL CONFERENCE ON THE MANAGEMENT OF NATURALLY OCCURRING RADIOACTIVE MATERIALS (NORM) IN INDUSTRY, october 19-30, 2020, Online. Abstract... 2020.

    Como referenciar este item
    Icon

  • IPEN-DOC 29762

    TRINDADE, FABIANE D.; DAMASCENO, SERGIO; OTUBO, LARISSA ; FLORIO, DANIEL Z.; FONSECA, FABIO ; FERLAUTO, ANDRE S.. Tuning of shape, disorder and oxygen vacancies in lanthanum-doped (0-70%) ceria shaped nanoparticles for oxidative coupling of methane. In: YILDIZ, BILGE (Org.) INTERNATIONAL CONFERENCE ON SOLID STATE IONICS, 23rd, July 17-22, 2022, Boston, MA. Abstract... 2022.

    Como referenciar este item
    Icon

  • IPEN-DOC 29761

    PATRICIO, NICOLE B. ; CARDOSO, JULIANO C. ; ESCOTE, MARCIA T.; LANFREDI, ALEXANDRE J.C.; SANTIAGO, ELISABETE I. . Electrochemical reduction of methane to value-added products using bifunctional CeO2/ZrO2 catalysts. In: YILDIZ, BILGE (Org.) INTERNATIONAL CONFERENCE ON SOLID STATE IONICS, 23rd, July 17-22, 2022, Boston, MA. Abstract... 2022.

    Como referenciar este item
    Icon

  • IPEN-DOC 29760

    OLIVEIRA, RAPHAEL A.M.P. de; FERLAUTO, ANDRE S.; TRINDADE, FABIANE D.; FLORIO, DANIEL Z.; FONSECA, FABIO . Effects of La Content in Ceria-Lanthana thin films prepared by pulsed laser deposition. In: YILDIZ, BILGE (Org.) INTERNATIONAL CONFERENCE ON SOLID STATE IONICS, 23rd, July 17-22, 2022, Boston, MA. Abstract... 2022.

    Como referenciar este item
    Icon

  • IPEN-DOC 29759

    BARBOSA, ANDREY S. ; BIANCOLLI, ANA L.G. ; LANFREDI, ALEXANDRE J.C.; RODRIGUES JR., ORLANDO ; FONSECA, FABIO ; SANTIAGO, ELISABETE I. . Effects of irradiation conditions on LDPE-Based anion exchange membranes properties—performance and stability. In: YILDIZ, BILGE (Org.) INTERNATIONAL CONFERENCE ON SOLID STATE IONICS, 23rd, July 17-22, 2022, Boston, MA. Abstract... 2022.

    Como referenciar este item
    Icon

  • IPEN-DOC 29757

    MELLO-CASTANHO, S. ; SILVA E COSTA, D.L. ; FUNGARO, D.A. ; VICENTE, R.; BORTOLOME, J.F.. Influência do nióbio na estrutura de vidros borossilicatos para uso nuclear. In: CONGRESSO BRASILEIRO DE ENGENHARIA E CIÊNCIA DOS MATERIAIS, 24., 6-10 de novembro, 2022, Águas de Lindóia, SP. Resumo... 2022.

    Abstract: A imobilização de rejeitos radioativos em vidros é uma técnica utilizada em muitos países que possuem programas de energia nuclear, constituindo uma importante rota de tratamento dos rejeitos radioativos de alta atividade, resultantes do reprocessamento do combustível nuclear. Este trabalho estuda a utilização de uma matriz vítrea com a adição de óxido de nióbio para a aplicação futura em imobilização de rejeitos radioativos de média e alta atividade. O estudo foi conduzido a partir de formulações de composições contendo teores de até 8,0 % em mol de Nb2O5 no sistema SiO2-Na2O-CaOB2O3-Al2O3. A funcionalidade do nióbio na estrutura de rede foi avaliada por meio de técnicas espectrométricas, de ensaios de irradiação ? e ? e de resistência hidrolítica, os quais forneceram informações importantes sobre a atuação do óxido na estrutura vítrea. Os resultados obtidos demonstram que a composição selecionada apresenta as especificações adequadas para incorporar e imobilizar elementos de radionuclídeos em sua estrutura de rede em nível atômico. Estas considerações foram obtidas utilizando-se as técnicas: espectroscopia vibratória no infravermelho por transformada de Fourier (FTIR), espectroscopia por ressonância magnética nuclear com rotação em ângulo mágico (MAS-RMN), análise térmica diferencial (ATD), irradiação por fontes de 241Am e por acelerador de feixe de elétrons (60Co) e ensaios de resistência hidrolítica. Os materiais vítreos obtidos são perfeitamente compatíveis com a aplicação proposta de imobilização de rejeitos radioativos por possuírem:superior estabilidade à radiação nuclear e notável resistência química. As pesquisas aqui desenvolvidas tiveram o apoio da Fundação de Amparo à Pesquisa do Estado de São Paulo, projeto n. 2018/10114-7.

    Como referenciar este item
    Icon

  • IPEN-DOC 29754

    FEITOSA, R.; ZAMBONI, C.B. ; SILVA, D.L.; PIMENTA D.C.; BARROS-BATTESTI D.M.; SANTIAGO, A.C.C.; SIMONS, S.M.. Análise do extrato das glândulas salivares de ornithodoros brasiliensis em colônias de alimentação artificial e em hospedeiro. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 21, 19-21 de outubro, 2022, Ilhéus, BA. Resumos... p. 297.

    Como referenciar este item
    Icon

  • IPEN-DOC 29751

    CORREA, E.L. ; BOSCH-SANTOS, B.; SALES, T.S.N. ; CABRERA-PASCA, G.A.; CORREA, B.S. ; CARBONARI, A.W. ; OLESHKO, V.P.; DENNIS, C.L.. Magnetic properties of Er-doped Fe3O4 nanoparticles studied by perturbed angular spectroscopy. In: RIVAS, MONTSERRAT (Ed.); BLANCO, JESUS A. (Ed.); GORRIA, PEDRO (Ed.) INTERNATIONAL CONFERENCE ON FINE PARTICLE MAGNETISM, 10th, May 26-31, 2019, Gijón, Spain. Proceedings... 2019. p. 133-133.

    Como referenciar este item
    Icon

  • IPEN-DOC 29671

    MIYAKAWA, WALTER; MASSI, MARCOS; RABELO NETO, JOSE da S. ; ZEZELL, DENISE M. . Caracterização de hidroxiapatita sintética por microscopia de força atômica. In: ENCONTRO NACIONAL DE FISICA DA MATERIA CONDENSADA, 31., 5-9 de maio, 2008, Águas de Lindóia, SP. Resumo... 2008.

    Como referenciar este item
    Icon

  • IPEN-DOC 29669

    LOPEZ, MARCIO A.P.A. ; BERECZKI, ALLAN ; WETTER, NIKLAUS U. . Nd:YAG polarized laser with beam quality beyond the birefringence limit and its application in a Singly-Resonant Optical Parametric Oscillator. In: SÃO PAULO SCHOOL OF ADVANCED SCIENCE ON FRONTIERS IN LASERS AND THEIR APPLICATIONS; JORGE ANDRÉ SWIECA SCHOOL ON NON LINEAR AND QUANTUM OPTICS, 16th, July 16-27, 2018, São Paulo, SP. Poster... 2018.

    Abstract: We demonstrate a simple, reliable and cheap high output power, linearly polarized and high-quality beam @1064 nm laser source, based on a previous work [i], where a Nd:YAG Diode-pumped Solid-state Laser (DPSSL) based on standard, commercial laser modules was presented. This kind of laser source is interesting for a large number of applications, such as pump laser for an Optical Parametric Oscillator (OPO) and frequency conversion, maintaining near-diffraction-limited beam quality factor (M2~1).

    Como referenciar este item
    Icon

  • IPEN-DOC 29661

    WETTER, NIKLAUS U. ; CAMARGO, FABIOLA de A. ; RANIERI, IZILDA M. ; BALDOCHI, SONIA L. ; LANDULFO, EDUARDO . Side-pumped, high beam-quality Nd:YLF amplifier for LIDAR applications. In: CONFERENCE ON LASERS AND ELECTRO-OPTICS EUROPE, June 12-17, 2005, Munich, Germany. Abstract... Piscataway, NJ, USA: IEEE, 2005. p. 53-53.

    Como referenciar este item
    Icon

  • IPEN-DOC 29660

    COURROL, LILIA C.; KASSAB, LUCIANA R.P.; DEL CACHO, VANESSA; WETTER, NIKLAUS U. ; GOMES, LAERCIO ; VIEIRA JUNIOR, NILSON D. ; MESSADDEQ, YOUNES; RIBEIRO, SIDNEY J.L.. Study of neodymium laser transition in glasses and influence of up-conversion processes under diode pumping. In: CONFERENCE ON LASERS AND ELECTRO-OPTICS EUROPE, June 22-27, 2003, Munich, Germany. Abstract... Piscataway, NJ, USA: IEEE, 2003. p. 406-407.

    Abstract: By solving the rate equations including upconversion for Nd glass hosts under continuous diode pumping, it was possible to calculate the nonlinear behavior of the pumping efficiency and threshold pump intensity as a function of exited-state population.

    Como referenciar este item
    Icon

  • IPEN-DOC 29659

    FUKUMOTO, MARCOS E.; WETTER, NIKLAUS U. ; KASSAB, LUCIANA R.P.; COURROL, LILIA C.. Optimum Yb3+ concentration in PbO-Bi2O3-Ga2O3 glasses for ultrashort laser applications. In: CONFERENCE ON LASERS AND ELECTRO-OPTICS EUROPE, June 22-27, 2003, Munich, Germany. Abstract... Piscataway, NJ, USA: IEEE, 2003. p. 404-405.

    Abstract: This study examines different Yb3+ doping levels in heavy metal oxide glasses showing interesting properties for laser applications at 1012 nm, mainly for short pulse generation under diode pumping.

    Como referenciar este item
    Icon

  • IPEN-DOC 29596

    SANTOS, PAULO R.S.; JESUS, ASHELY A.S.S. de; LIMA, WILLIAM B. de; COSTA, ISRAEL F.; FAUSTINO, WAGNER M.; FELINTO, MARIA C.F.C. ; TEOTONIO, ERCULES E.S.; BRITO, HERMI F.. Complex of luminescent europium containing bis(diphenylphosphine)oxide ligands. In: SILVA, FERNANDO de C. da (Org.) REUNIAO ANUAL DA SBQ, 45., 31 de maio - 3 de junho, 2022, Maceió, AL. Resumo... São Carlos, SP: Aptor Software, 2022. p. 380-380.

    Abstract: Trivalent lanthanide coordination compounds are characterized by their long luminescence lifetime of emitting level, narrow emission band and high color purity, which makes them fascinate for application in LCMDs, OLEDs, immunoassay, bioimaging probes, luminescent sensors and in telecommunications systems etc. Among this class of compounds, those ones in which β-diketonate ligands act as luminescence sensitizers have found a prominent position. However, most of these systems are obtained in the form of simple molecular entities. Recently, interest in new polynuclear systems containing diketonate ligands has been growing significantly. Therefore, this work reports on the synthesis, characterization, and photophysical properties of the lanthanide compounds of general formula [Ln2(β- dik)6(dppeO2)], where β-dik = tta, bzac, dbm, [Ln(β-dik)3(dppeO2)] where β-dik = btf and [Ln(β-dik)3(dppeO2)2]n where β-dik = fod, dppeO2 = 1,2-Bis(diphenylphosphino)ethane oxide, Ln = Eu3+ and Gd3+. These complexes were prepared by direct reaction among ethanolic solutions of the [Ln(β-dik)3(H2O)] complexes and dppeO2 ligands in the molar ratios [Ln(β-dik)3(H2O)]: dppeO2 of 2:1. The FT-IR spectra of complexes show red-shifted of 20–41 cm-1 for the band assigned to the ν(C=O) vibrational mode (Fig 1a), suggesting that β–diketonate ligands are coordinated to the Ln3+ ion in chelating mode. Diffuse reflectance spectra of the Eu3+-complexes show strong absorption bands in the range of 200–450 nm ascribed to the S0→Sn (ππ* or mixed nπ*) transitions from the diketonate ligands. The high relative intensities between excitation bands from ligand and 4f-4f transitions in the complexes with tta, btf and bzac indicates an efficient ligand-to-metal energy transfer process. On the other hand, for complexes with fod, dbm and bzac (Fig 1b) a lower intensity of the ligand bands is observed, suggesting that a luminescence suppression channel is operative. The emission spectra present the bands assigned to the 5D0→7FJ (J = 0–4) transitions of the europium ion (Fig 1c). The Ω2 and Ω4 intensity parameters, lifetime (τ) and luminescence intrinsic quantum yield ΦEu/Eu were calculated from the emission spectral data and luminescence decay curves of the compounds in solid state (Table 1). According with these data, the polymeric or dimeric investigated systems exhibit high luminescence intensities in the red region, which make them potential candidates for application as emitting layer in molecular light-converting devices.

    Como referenciar este item
    Icon

  • IPEN-DOC 29595

    SAULA, MATHEUS S.N.; FELINTO, MARIA C.F.C. ; NUNES, LUIZ A.O.; MALTA, OSCAR L.; BRITO, HERMI F.. Europium activator ion acting as an efficient luminescent probe to identify local symmetry in Li2ZnSn3O8:Eu3+ materials prepared by the MASS method. In: SILVA, FERNANDO de C. da (Org.) REUNIAO ANUAL DA SBQ, 45., 31 de maio - 3 de junho, 2022, Maceió, AL. Resumo... São Carlos, SP: Aptor Software, 2022. p. 390-390.

    Abstract: Luminescent materials doped with rare earth ions (RE) have been intensely applied in several areas in the new photonic technologies, such as emergency lights, radiation detectors, biological markers, and anti-counterfeiting products. Stannate-based host matrices have attracted significant attention, as they are relatively inexpensive and have a favorable bandgap to allow the persistence luminescence phenomenon. In this work, Li2ZnSn3O8:Eu3+ was synthesized by the microwave-assisted solid-state method (MASS) using a domestic microwave oven after gridding Li2CO3, ZnO, SnO2, Eu2O3 precursor oxides. Therefore, the as-prepared precursors were heated in a static air atmosphere at a preset configuration of 900W for 20 min. The material was characterized by powder X-ray diffraction (PXRD), scan electron microscopy (SEM), and diffused reflectance spectroscopy. The PXRD patterns indicate the formation of the desired Li2ZnSn3O8matrix phase when doped with different concentrations of the Eu3+ ion, showing a high crystallinity. The photoluminescence properties were determined based on the emission spectra of the Li2ZnSn3O8:mol%Eu3+ materials (Fig 1), showing intense yellowish-orange and reddish-orange emission colors under UV excitation at 282 and 336 nm, respectively. Moreover, both spectra show narrow emission bands characteristic of 5D0 →7F0-4 Eu3+ transitions. Sinceeuropium ions are powerful spectroscopic probes for the symmetry of the first coordination sphere of these ions in a lattice, and the spectral profiles change with the incident radiation wavelengths, which demonstrates the presence of more than one site of symmetry around the chemical environment of the Eu3+ion.Finally, it is noteworthy that these luminescent materials can be selectively excited in the UV range, leading to more than one specific emission spectral profile, which can be applied as efficient luminescent probes for anti-counterfeiting products.

    Como referenciar este item
    Icon

  • IPEN-DOC 29594

    COSTA, ISRAEL F.; BLOIS, LUCCA; SANTOS, PAULO R.; CARNEIRO NETO, ALBANO N.; MOURA JUNIOR, RENALDO T.; DEFLON, VICTOR M.; CARLOS, LUIS D.; FAUSTINO, WAGNER M.; FELINTO, MARIA C.F.C. ; LONGO, RICARDO L.; MALTA, OSCAR L.; TEOTONIO, ERCULES E.S.; BRITO, HERMI F.. Unexpected luminescent and thermal properties of novel tetrakis Eu3+- indandionate complex: experimental and theoretical studies. In: SILVA, FERNANDO de C. da (Org.) REUNIAO ANUAL DA SBQ, 45., 31 de maio - 3 de junho, 2022, Maceió, AL. Resumo... São Carlos, SP: Aptor Software, 2022. p. 437-437.

    Abstract: Luminescent coordination compounds based on trivalent europium ions (Eu3+) have found wide range of applications in new technologies, such as optoelectronics, molecular thermometers, and biomedical devices. Applications of this materials may be associated with narrow emission bands, which are arise from intra-configurational Laporte forbidden 4f – 4f transitions (5D0 → 7FJ). Furthermore, Eu3+ ion can act as a powerful spectroscopic probe and the 5D0 → 7F2 transition are strongly sensitive to small angular variations in the coordination polyhedron. However, the energy structures of the organic ligands in the complexes play the most important role on the Eu3+ luminescence sensitization process1. In this context, this work reports about theoretical, syntheses, characterization, and photoluminescence studies of a series of tetrakis complexes containing tetraethylammonium, Et4N+[Ln(L)4]– (Et4N+: tetraethylammonium cation, Ln: Gd and Eu, and L: 2-acyl-1,3-indandionate). The Judd–Ofelt intensity parameter (Ωλ), lifetime (τ), radiative (Arad) and non-radiative (Anrad) coefficients, and intrinsic quantum yield ΩEu/Eu) values were calculated for different temperatures (80 - 475 K) (Table 1). The Et4N+[Eu(isovind)4]– complex, where isovind: 2-isovaleryl-1,3-indandonate) shows an extraordinarily high value of the intensity parameter (Ω2 = 73.5×10-20 cm2, considering the index of refraction equal to 1.5) and radiative decay rate (Arad = 2.468×103 s-1) at 300 K. These optical results show an abnormally high emission intensity of the 5D0 → 7F2 transition (Figure 1), leading to the highest measured Ω2 value of the europium materials, to the best our knowledge, reported in the literature. In addition, this complex shows different solid phases at 367 and 460 K, leading significant changes in the band profile assigned to the 5D0 → 7F2 transition. The spectroscopic study of luminescent systems has shown very interesting and promising results for applications, such as OLED devices and luminescent thermometers based on Ln3+ ions.

    Como referenciar este item
    Icon

  • IPEN-DOC 29593

    FELINTO, MARIA C.F.C. ; SILVA, JAQUELINE A.P. ; FRANCISCO, LEONARDO C. ; COSTA, ISRAEL F.; TEOTONIO, ERCULES E.S.; MALTA, OSCAR M.L.; BRITO, HERMI F.. Preparation and photoluminescence properties of functionalized silica submicron-sphere materials decorated with Eu(tta)3•FX complex (FX= fluoxetin). In: SILVA, FERNANDO de C. da (Org.) REUNIAO ANUAL DA SBQ, 45., 31 de maio - 3 de junho, 2022, Maceió, AL. Resumo... São Carlos, SP: Aptor Software, 2022. p. 511.

    Abstract: The development of functionalized silica particles containing luminescent materials has received special attention because of their biological applications such as optical markers in vitro and in vivo, clinical diagnosis and drug delivery. The incorporation of Eu3+-complex into modified silica particles combines optical characteristics of Eu3+- complex and properties of inorganic oxides, resulting in organic-inorganic hybrid materials. In this work, we synthesize and characterize sub-microspheres of SiO2 decorated with a new complex of Eu3+ and fluoxetine, an antidepressant of the selective serotonin reuptake inhibitor (SSRI) class. The Eu-complex and the submicron-spheres (ϕ~500nm) were characterized optically and photo-physical characteristics of these materials were examined spectroscopically. In emission spectra of complex (Fig 1.a), four characteristic peaks of Eu3+ ion with the maximum at ~580 nm, ~595 nm, ~614 nm, and ~702 nm accredited to 5D0 → 7FJ (J = 0-4) transitions appeared upon excitation in UV region. The most intense peak at 614 nm is accountable for the bright red emission of the ternary complex. For the SiO2 nanospheres decorated with the complex these transitions are close to the complex but are identified the change in the symmetry around the Eu3+ ion when analyzing the spectra. Experimental intensity parameters (Ωλ), lifetime (τ), radiative (Arad) and non-radiative (Anrad) coefficients, and intrinsic quantum yield (Q Eu3+/Eu3+) values were determined. 3D emission spectra for the Eu(tta)3•FX complex in the VUV region (Fig. 1b) revealed a high emission band originated from (Eu3+) 5D0→7F2,4 transitions (centered around 614 and 702nm) under excitation at near bandgap energy. Color purity and CIE parameters also suggest the red luminous behavior of complex. Thermal and morphological behavior of the Eu-complex and submicron-spheres decorated with the Eu(tta)3•FX complex are also evaluated (Fig1c). Our investigation has revealed that the synthesized complex and inorganic hybrid materials could be used in preparing lighting systems, OLEDs, display devices and biological sensors owing to their luminescent characteristics

    Como referenciar este item
    Icon

  • IPEN-DOC 29592

    SANTOS, SIMEI T.S.; BRITO, HERMI F.; TEOTONIO, HERCULES E.S.; FELINTO, MARIA C.F.C. ; MALTA, OSCAR L.. Synthesis, modification surface and characterization of tungstate/molybdate of zinc, doped with lanthanide ions [ Zn1-xREx(WO4)y(MoO4)z] (y or z = 0, 0.5, 1). In: SILVA, FERNANDO de C. da (Org.) REUNIAO ANUAL DA SBQ, 45., 31 de maio - 3 de junho, 2022, Maceió, AL. Resumo... São Carlos, SP: Aptor Software, 2022. p. 535-535.

    Abstract: A class of materials with the general formulation of MIMIII(XO4)2 (MI=Alkali metal, MIII = trivalente metal and X = W or Mo) with structural importance and diferente properties exposed to the attention of researchers, especially when doped with rare earth ions, which give the material luminescent properties. These properties can make the material promisingly important for diverse applications, such as solar cells, light converting devices and sensors, depending only on the changes in its structure and surface. This work was synthesized using co-precipitation method from aqueous solutions tungstates and molybdates of zinc doped with rare earth íons (Scheme 1a [Zn0.95Eu0.05WO4],1b [Zn0.95Eu0.05(WO4)0.5(MoO4)0.5],1c [Zn0.95Eu0.025Tb0.025WO4]) and coated with a thin layer of sílica (scheme 1d) using the hydrolysis and condensation of tetraethoxysilane (TEOS). For the phenantroline ligand functionalization, 3- (chloropropyl)triethoxy-silane (CPES) was grafted onto the particle surfaces in their aqueous suspensions, and bound to modified phenanthroline. For the synthesis of doped tungstates and molybdates, equivalent amounts of zinc, terbium, and europium chlorides were added under stirring at 70 °C for 1h. After drying, the material was ground with TEOS, water, ethanol and remained (with catalyze acid) for 3h at 70 °C under constant agitation. The 1,10- Phenantroline (scheme 1e) has been nitrated (H2SO4/HNO3) (scheme 1f) and reduced (Sn/HCl) (scheme 1g and infrared) to then be coupled to the modified particle. The final product was characterized. We are currently improving and studying synthesis conditions and aplications.

    Como referenciar este item
    Icon

  • IPEN-DOC 29591

    RUSSO, DANIELA C.; AMARAL, KLEICY C. ; CALDAS, LHAIS A.; VIEIRA, DANIEL P. ; SARTORELLI, PATRICIA; RIBEIRO FILHO, WALDEMAR A.. Cytototic activity and chemical profile of methanolic extract obtained from Avelós stem (Euphorbia tirucalli L.) Euphorbiaceae. In: SILVA, FERNANDO de C. da (Org.) REUNIAO ANUAL DA SBQ, 45., 31 de maio - 3 de junho, 2022, Maceió, AL. Resumo... São Carlos, SP: Aptor Software, 2022. p. 712-712.

    Abstract: Avelós (Euphorbia tirucalli Linnaeus), a plant selected for this study, has been popularly used in the fight against tumors, arousing the interest of researchers in this area so that it can be used safely in the auxiliary treatment of different types of cancer.1 It belongs to the family Euphorbiaceae and to the genus Euphorbia, it is also the object of studies related to the treatment of a range of infectious and inflammatory diseases. Herbaceous much used by popular and traditional medicine, presents, a latex rich in molecules that confirm its high toxicity. The objective of this research was to verify the chemical profile of the methanolic extract obtained from the stem (modified leaves) of the plant in question and to determine the cytotoxicity of the crude extract by cytotoxic assay against the lineages of adenocarcinoma (MCF-7) and neoplastic cells of human melanoma (SK-MEL-37).2 For this, the plant was collected, and after drying and milling the material was extracted with methanol. Subsequently, the present compounds were separated by the thin-layer chromatography technique and the classes of substances found in the extract were identified by the technique of Nuclear Magnetic Resonance of Hydrogen and Carbon-13 (NMR).3 The combination of cyclohexane with Acetone and Hexane (5:3:2) provided a suitable polarity for the elution of the extract, which was revealed with ultraviolet detection and different reagents: sulfuric acid solutions; aluminum chloride; ferric chloride; 10% potassium hydroxide in ethanol; green bromocresol indicator solution; potassium permanganate, Dragendorff Reagent, vanillin and iodine vapors. The phytochemical study of the methanolic extract of Euphorbia tirucalli allowed to identify the presence of phenolic compounds, flavonoids and terpenes, a result confirmed by NMR spectra. The cytotoxic potential assays, although they are in low concentration thus altering the result, show that the methanolic extract of Euphorbia tirucalli shows activity against the tested cell lines. The observed activity may be related, according to information available in the literature, with the classes identified in the samples studied.

    Como referenciar este item
    Icon

  • IPEN-DOC 29590

    RAMOS, LUIZ D.; MANTOVANI, MARIANA C. ; SARTORI, ADRIANO; DUTRA, FERNANDO; STEVANI, CASSIUS V.; BECHARA, ETELVINO J.H. Aerobic co-oxidation of hemoglobin and aminoacetone. In: SILVA, FERNANDO de C. da (Org.) REUNIAO ANUAL DA SBQ, 45., 31 de maio - 3 de junho, 2022, Maceió, AL. Resumo... São Carlos, SP: Aptor Software, 2022. p. 164-164.

    Abstract: Aminoacetone (1-aminopropan-2-one), a putative minor biological source of methylglyoxal, reacts like other 􀄮-aminoketones such as 6-aminolevulinic acid (first heme precursor) yielding electrophilic 􀄮-oxoaldehydes, ammonium ion and reactive oxygen species by metal- and hemeprotein catalyzed aerobic oxidation 1,2. A wealth of reports implicates methylglyoxal in protein crosslinking and DNA addition, leading to age-related disorders, including diabetes 3,4. Importantly, methylglyoxal-treated hemoglobin adds four water-exposed arginine residues, which may compromise its physiological role and potentially serve as biomarkers for diabetes 5. In this work, we investigate the co-oxidation of aminoacetone and oxyhemoglobin in normally aerated phosphate buffer, leading to structural changes in hemoglobin, which might reportedly be attributed to the addition of aminoacetone-generated methylglyoxal to the protein. Hydroxyl radical-promoted chemical damage to hemoglobin may also occur in parallel, which is suggested by EPR-spin trapping studies with 5,5-dimethyl-1-pyrroline-N-oxide and ethanol. Concomitantly, oxyhemoglobin is oxidized to methemoglobin, as indicated by characteristic CD spectral changes in the absorption Soret and visible regions. Overall, these findings may contribute to elucidate the molecular mechanisms underlying human diseases associated with hemoglobin dysfunctions (e.g., diabetes) and with aminoacetone in metabolic alterations related to excess of glycine and threonine (e.g., threoninemia, cri-du-chat syndrome).

    Como referenciar este item
    Icon

  • IPEN-DOC 29589

    PAPAI, RODRIGO; ALMEIDA, GILMAR A. ; SILVEIRA, JOAO R.F.; SILVA, ANDRE L.N.; SANTOS, CELIA A.L.; NAGASIMA, THIAGO P.; JABES, EDUARDO G.; LANDGRAF, FERNANDO J.; LUZ, MACIEL S.. Proposal of an analytical method for Nd, Pr, Fe and B determination in super-magnet alloys by ICP OES. In: SILVA, FERNANDO de C. da (Org.) REUNIAO ANUAL DA SBQ, 45., 31 de maio - 3 de junho, 2022, Maceió, AL. Resumo... São Carlos, SP: Aptor Software, 2022. p. 132-132.

    Abstract: Present in motors, electric turbines and data storage devices (hard disk), magnets are indispensable in the manufacture of computers, televisions, cell phones, smart watches and several modern electronic devices. The chemical composition of the magnet directly influences its magnetic performance and consequently impacts the performance of the products with which it is associated. In this context, the knowledge of the majority chemical composition of these materials contributes to an efficient quality control in the act of production and helps the producers of the high-technology industry to select the magnets in order to maintain a uniformity of these materials. Although there are several types of magnets, those that use rare earth elements generally have strong magnetic activity, and are often called super-magnets. Among the rare earth elements, the mixture of neodymium and praseodymium (didymium) is widely used in metal alloys together with the elements iron and boron for its performance as a supermagnet. The (Nd,Pr)FeB alloy production consists, in summary, to obtain metallic didymium by electrolytic reduction of didymium oxide (mixture of neodymium and praseodymium oxides) followed by iron and boron elements incorporation. The chemical composition control is important to avoid undesirable phases, such alpha-iron, and to control the microstructure formation during the alloy manufacturing step. The chemical composition range must be optimized such that minimizes the use of rare earth elements (Nd and Pr). In this context, this work evaluated the best instrumental conditions for the determination of alloy elements by ICP OES. Interference studies were carried out and the developed method was validated through interlaboratory tests and addition and recovery tests.

    Como referenciar este item
    Icon

  • IPEN-DOC 29575

    CASTRO, PEDRO ; PEREIRA, DAISA ; ANA, PATRICIA; MATOS, CHRISTIANO; ZEZELL, DENISE . Q-switched Nd:YAG laser on dental enamel with photoabsorber: a confocal Raman pilot study. In: INTERNATIONAL CONFERENCE ON LASER APPLICATIONS IN LIFE SCIENCES, 16th, April 1-2, 2022, Nancy, France. Abstract... Nancy, France: PROGEPI, 2022. p. 124-124.

    Abstract: Nd:YAG lasers emitting λ=1064 nm at microsecond and nanosecond pulses are alternatives to prevent dental caries and erosion in clinics.This wavelength allows most of photons to penetrate deep in the hard tissue due to low absorption of hydroxyapatite in the region. It is necessary to use photoabsorbers so most of photons are absorbed in the surface of the tissue preventing dental pulp necrosis. Currently the coal paste is used as a photoabsorber but the irradiated tissue turns darker what implies in the patients low adherence to the treatment due to aesthetic reasons. [1,2]. Confocal Raman spectroscopy is a non-destructive optical method to obtain detailed information about molecular composition of biological structures in depth. The most prominent feature of Confocal Raman spectroscopy is the reliable capability to provide the biomolecular data with no use of ionizing radiation to penetrate in the sample. This work aims to characterize the dental enamel irradiated with Nd:YAG laser with nanoseconds pulses, in order to describe the depth related changes promoted in the enamel, by the heat generated due to laser irradiation. For these measurements, 30bovine enamel blocks of 8 mm2, were randomized into 3 groups: G1 – enamel untreated; G2 – enamel irradiated with Nd:YAG nanopulsed laser (1064 nm, 4 W; 1,05 J/cm2; 5 ns 20 Hz, Brilliant, Quantel Laser) using a coal paste as photoabsorber; G3 -enamel irradiated with Nd:YAG nanopulsed laser ( same parameters as G2) using squid ink as photoabsorber. The assessments of three different depth regions of the cubic shaped samples were: region A- left corner above of the sample, region B- middle of the sample and Region C- right corner below of the sample. The intensity map of phosphate (950 cm-1)regarding the position, were calculated [3,4] as shown in the Figure 01. The comparative results in the Fig.1 demonstrated that application of coal paste associated with Nd:YAG (G2) can preserve the inorganic content better than the squid ink group (G3). These findings have crucial clinical implications in the laser protocol development and itwas possible to correlate the heat penetration depth of the laser irradiation with photoabsorber using the images obtained bythe confocal Raman.

    Palavras-Chave: dentistry; dentin; enamels; lasers; neodymium lasers; yttrium compounds

    Como referenciar este item
    Icon

  • IPEN-DOC 29574

    DEL-VALLE, MATHEUS ; CARVALHO, MARCELO; SANTOS, MOISES ; PINTO, NATHALI; JATENE, FABIO; POMERANTZEFF, PABLO; BRANDAO, CARLOS; ZEZELL, DENISE . Evaluation of calcified mitral valves after Er,Cr:YSGG irradiation using Optical Coherence Tomography. In: INTERNATIONAL CONFERENCE ON LASER APPLICATIONS IN LIFE SCIENCES, 16th, April 1-2, 2022, Nancy, France. Abstract... Nancy, France: PROGEPI, 2022. p. 152-152.

    Abstract: Mitral valve is responsible to control the left atrium-ventricle blood flux. Mitral stenosis is a disease that occurs in consequence of calcification and fibrosis on the cuspids of the valve. Diagnosis can be performed using echocardiography.Many treatments are possible, and one of them is commissurotomy (surgical approach).High intensity laser irradiation may be a new strategy for this surgical technique[1], and the optical coherence tomography (OCT) may contribute to the valve evaluation[2], asit provides higherspatialresolutionin exchange of lower penetrationthan ultrasonography. In this way, the aim of this study is to evaluate laser irradiation effectsincalcified mitral valvesusing OCTand digital processing.To that, it was conducted an ex-vivostudywith four human mitral valvessamples,obtained from valve replacement surgeries in the Heart Institute.The samples were splitin four groups: scalpel cut, laser cut, scalpel debridement and laser debridement.Cutting and debridement procedures were performed in calcified regions of the valves, usinga disposable scalpelbladeand anEr,Cr:YSGG laser(Waterlase; Biolase Inc., CA, USA), emitting at 2780 nm. The laser parameters were set at power = 1.6W, frequency = 20 Hz, energy density = 28.3J/cm2,pulse duration = 700 μs, 15% of water and 15% of air.The imaging was performed using a spectraldomain OCT system(Callisto110C1;ThorLabs Inc., NJ, USA).It was acquired10 B-scans per sample, 5 inprocedures regions and 5 in sound regions. The Optical Attenuation Coefficient (OAC) was calculated by comparing a beer-lambert like equation to exponential fittings of the A-scans[3].The distribution and normality of variances were tested using Shapiro-Wilk test,and statistical comparison was performed using one-way ANOVA and Tukey’s post hoc. All tests considered a level of significance of 5%.The FigureAshows a representative B-scan of a visibly calcified region, where a pattern of higher intensities can be observed.Thispattern is related tomorphological and optical changes, mainly a refractive index change, due to calcium presence in the valve tissue.This B-scan was acquired only to understand the calcified tissue aspect, as the procedures regions does notpresent visibly largecalcium stones.The Figure Bshowsthe statisticalanalysis, where the sound OAC values, as a mean of all sound regions, presented a significant statistical difference in comparison to scalpel groups, while no difference waspresentedin relation to laser groups. Higher OAC values are related to anaugmentation of the light backscatteringdue to calcium refractive index, leading to a change of lightpropagation in tissue-calcium interfaces.This finding indicates thatthe laser procedures promoted a better removal of calcified tissue than the scalpelmethods, which can be related to tissue-ablation interaction.Furthermore, the statistical difference between scalpel cut group and both laser groups suggests that the scalpel needs more wear interaction with the tissue, such as in the debridement procedure, being unable to significatively remove the calcification in a single cut.This study points the Er,Cr:YSGG and the OCT as potential techniques for the calcified tissue removal and evaluation,respectively, duringmitral valvessurgeries, although further studieswith higher sample numbermust be performed.

    Palavras-Chave: cardiovascular system; valves; cardiovascular diseases; fibrosis; tomography; coherent radiation

    Como referenciar este item
    Icon

  • IPEN-DOC 29573

    ZEZELL, DENISE ; CASTRO, PEDRO ; DEL-VALLE, MATHEUS ; CAMILLO-SILVA, CARLOS ; SAMAD, RICARDO ; DE ROSSI, WAGNER ; SANTOS, MOISES . FTIR imaging on glass substrates evaluation of histological skin burn injuries specimens treated by femtosecond laser pulses. In: INTERNATIONAL CONFERENCE ON LASER APPLICATIONS IN LIFE SCIENCES, 16th, April 1-2, 2022, Nancy, France. Abstract... Nancy, France: PROGEPI, 2022. p. 205-205.

    Abstract: Burn injuries continue to be one of the leading causes of unintentional death and injury in low- and middle-income countries [1]. Burns are considered an important public health problem, because in addition to physical problems that can lead the patient to death, they cause psychological and social damage. An estimated 180,000 deaths every year are caused by burns [2]. The use of infrared (IR) spectroscopy for studying biological specimens is nowadays a wide and active area of research. The IR microspectroscopy has proved to be an ideal tool for investigating the biochemical composition of biological samples at the microscopic scale, as well as its fast, sensitive, and label-free nature [3]. IR image spectral histopathology has shown great promise as an important diagnostic tool, with the potential to complement current pathological methods, reducing subjectivity in biopsy samples analysis. However, the use of IR transmissive substrates which are both fragile and prohibitively very expensive, hinder the clinical translation. The goal of this study is to evaluate the potential of discriminating healing process, in burned skin specimens treated with ultrashort pulses laser 3 days after the burn. This study is considering a previous paper [4], in which it analyzed only micro-ATR-FTIR spectra of a frozen sample point. The specimens were obtained from third degree burn wound. The wounds treatment were performed three days after the burn, and the animals were sacrificed 3 and 14 days post-treatment. Using coverslipped H&E stained tissue on glass from previous histopathological analysis and applying the analytical techniques PCA and K-means on N−H, O−H, and C−H stretching regions occurring at 2500−3800 cm−1 (high wavenumber region), were possible to discriminate burned epidermal and dermal regions from irradiated in same regions on sample. In the figures is shown the average spectrum at (a) day 3 and (b) day 14. , in both there were increase of burned+laser treated bands. The great potential of this study was to analyse coverslipped H&E stained tissue on glass, without compromising the histopathologist practices and contribute for clinical translation.

    Palavras-Chave: burns; injuries; animal tissues; infrared spectra; healing; lasers; pulses

    Como referenciar este item
    Icon

  • IPEN-DOC 29572

    FAROOQ, SAJID ; DEL-VALLE, MATHEUS ; SANTOS, MOISES O. dos ; NASCIMENTO, SOFIA ; BERNARDES, EMERSON S. ; ZEZELL, DENISE M. . Breast cancer subtypes diagnostic via high performance supervised machine learning. In: INTERNATIONAL CONFERENCE ON CLINICAL SPECTROSCOPY, 12th, June 19-23, 2022, Dublin, Ireland. Abstract... 2022.

    Abstract: Aim: Breast cancer molecular subtypes are being used to improve clinical decision. The Fourier transform infrared (FTIR) spectroscopic imaging, which is a powerful and non-destructive technique, allows performing a non-perturbative and labelling free extraction of biochemical information towards diagnosis and evaluation for cell functionality. However, methods of measurements of large areas of cells demand a long time to achieve high quality images, making its clinical use impractical because of speed of data acquisition and dearth of optimized computational procedures. In order to cope with these challenges, Machine learning (ML) technologies can facilitate to obtain accurate prognosis of Breast Cancer (BC) subtypes with high action ability and accuracy. Methods: Here we propose a ML algorithm based method to distinguish computationally BC cell lines. The method is developed by coupling K neighbors Classifier (KNN) with Neighborhood Component Analysis (NCA) and NCA-KNN methods enables to identify BC subtypes without increasing model size as well additional parameters. Results: By incorporating FTIR imaging data, we show that using NCA-KNN method, the classification accuracies, specificities and sensitivities improve up to 97%, even at very low co-added scan (S_4). Moreover, a clear distinctive accuracy difference of our proposed method was obtained in comparison with other ML supervised models. Conclusion: For confirming our model results performance, the cross validation (k fold = 10) and receiver operation characteristics (ROC) curve were used and found in great agreement, suggest a potential diagnostic method for BC subtypes, even with small co-added scan < 8 at low spectral resolution (4 cm-1).

    Como referenciar este item
    Icon

  • IPEN-DOC 29571

    CASTRO, P. ; SILVA, C. ; ZEZELL, D. . Burn wound healing by infrared spectroscopy imaging: a pilot study. In: INTERNATIONAL CONFERENCE ON CLINICAL SPECTROSCOPY, 12th, June 19-23, 2022, Dublin, Ireland. Abstract... 2022.

    Abstract: Aim: Burns are one of the major causes of morbidity and the most costly traumatic injuries worldwide1. The traditional techniques used to assess the biochemical events related to wound repair are laborious, time-consuming and require multiple staining. Thus, the present study aims to evaluate the feasibility of Fourier transform infrared (FTIR) spectroscopy in order to monitor the progress and healing status of burn wounds. Methods : Third-degree burn injuries were induced on Wistar rats by water vapor exposure. Afterwards, biopsies specimen was extracted for further histopathological examination and IR imaging evaluation at 7 days. The spectral imaging was performed using a micro- FTIR equipment in transflection mode (MirrIR, Kevley Technologies), with a 32 x 32 FPA of 5.5 μm pixel size. All spectra data were baseline corrected and vector normalized. The preprocessing and image comparison was performed using Cytospec software (Cytospec, version 2.00.5)2. Results : The pairwise analysis was evaluated in the wavenumber region maps of 1200-1300 cm-1. The biomolecule band associated to collagen content (1200 – 1300 cm-1) was most prominent3 on the 7th day image in the post-burn that in healthy group. Conclusions : Therefore, our pairwise comparison revealed that metabolic activity induced by thermal injury increases the chemical activity associated to the healing progresses. Our findings show that FTIR spectroscopy has potential to identify the biochemical signatures induced by burn injury.

    Como referenciar este item
    Icon

  • IPEN-DOC 29570

    JANUARIO, ELIANE R. ; VAZ, JORGE M. ; SPINACE, ESTEVAM V. . Photocatalytic oxidation of methane coupling with hydrogen evolution from water over Au decorated Ga2O3 catalysts. In: CINE CONFERENCE, 3., 18-19 de outubro, 2022, Campinas, SP. Resumo... 2022.

    Abstract: Methane is a major constituent of natural gases and is an important source of carbon and hydrogen for the chemical industry. However, CH4 is one of the most stable molecule and high reaction temperatures are required to transform CH4 into more valuable chemicals [1]. In this work, we investigated the use of β-Ga2O3 loaded with Au nanoparticles (0.1–1.0%) as photocatalysts that were prepared 3 different methods: pre-formed NNTS; in-situ; and H2 reduction. The materials were characterized by XRD, UV-Vis, TEM, and Raman. The reactions were performed on a photocatalytic reactor with Hg lamp (450W, UV/A/B/C). CH4 gas was bubbled into H2O, Au/Ga2O3 in suspension and the products were identified by GC-MS and quantified by GC-FID/TCD using calibration curves. C2H6, CO2, H2 with minor quantities of C2H4, C3H8, C4H10, and CO were produced. The best performance was observed for the photocatalyst prepared with 0.03% of Au that produced 112 mol.gcat-1h-1 of C2H6 and 16.500 mol.gcat-1h-1 of H2.

    Palavras-Chave: methane; photocatalysis; gold; nanoparticles

    Como referenciar este item
    Icon

  • IPEN-DOC 29569

    CARMINATI, S.A. ; VAZ, J.M. ; SPINACE, E.V. . Photocatalytic coupling of methane over TiO2/WO3 heterojunction photocatalysts to enhance the production of ethane and hydrogen. In: CINE CONFERENCE, 3., 18-19 de outubro, 2022, Campinas, SP. Poster... 2022.

    Palavras-Chave: methane; photocatalysis; titanium oxides; tungsten oxides

    Como referenciar este item
    Icon

  • IPEN-DOC 29568

    SILVAINO, PATRICIA F. ; SPINACE, ESTEVAM V. ; VAZ, JORGE M. . Development of noble metals/TiO2 photocatalysts for photocatalytic conversion of methane coupling with hydrogen evolution from water. In: CINE CONFERENCE, 3., 18-19 de outubro, 2022, Campinas, SP. Resumo... 2022.

    Abstract: Methane, the main component of natural gas (< 80%), is an expressive source of carbon and hydrogen, with large world reserves and can be used as raw material to produce petrochemicals and fuels; however, efficient CH4 conversion under mild conditions remains a challenge due to its low reactivity. In addition, the methane conversion coupled with water splitting, which is the purpose of this work, is quite interesting and desirable, due to the production of hydrogen in a more sustainable way. One of the alternative ways to convert CH4 under low temperatures is Heterogeneous Photocatalysis, which will be used in the present study. When a semiconductor photocatalyst absorbs light with a wavelength greater than or equal to its bandgap, electrons are photoexcited to the conduction band and holes (h+) are formed in the valence band, forming the so-called electron-hole pairs, which in turn can initiate various redox reactions (Figure 1). Solar Photocatalysis would be an ideal method to convert methane and produce hydrogen from water. In this project, photocatalysts with different compositions and morphologies will be developed based on noble metals nanoparticles (Pt, Pd, Au, Ag) supported on TiO2 P25. The final goal is to obtain more active photocatalysts to increase the quantum efficiency of the system.

    Palavras-Chave: methane; photocatalysis; semiconductor materials; hydrogen production

    Como referenciar este item
    Icon

  • IPEN-DOC 29565

    OTOMO, JULIANA I. ; GIMENEZ, MAISE P. ; ANDRADE, MARIANA N. de ; MONTEIRO, LUCILENA R. ; NASCIMENTO, LETICIA da S. ; BATAGLIA, HENRIQUE ; LEAO, PAULO H.B. ; CECILIO, PRISCILA de S. ; BERGAMASCHI, VANDERLEI ; COUTINHO, JOAO F. ; BUSTILLOS, JOSE O.W.V. . Optimizations on Lithium ion exchange separation and isotopic measurements. In: SIMIONATO, ANA V.C. (Coord.) IBEROAMERICAN CONFERENCE ON MASS SPECTROMETRY, 3rd, December 10-15, 2022, Rio de Janeiro, RJ. Abstract... Campinas, SP: Sociedade Brasileira de Espectrometria de Massas - BrMASS, 2022. p. 389-390.

    Abstract: Introduction: The Lithium-7 is of interest for nuclear application, being used for primary cooling of PWR (Pressurized Water Reactor) reactors [1]. An environmentally friendly technique is required to replace the Mercury amalgam technique used worldwide [1,2]. This work aims to present the preliminary results of the development of 7Li separation by ion exchange. Methods: A 120 mm x1.0 cm i.d. glass column filled with Dowex 50W-x16 resin was used. A total of 3.0 liters of 0.2 M CH3COOLi solution percolated the column in order to saturate and displace the formed band of 6Li and 7Li. Fractions were collected every 50 mL, then the resin was washed with 5M HNO3 and ultrapure water. The fractions were filtered and evaporated at 80°C, finally taken up with HNO3 1%. Samples were analyzed by ICP-OES – SPECTRO ARCOS. The fractions were analyzed by ICP-MS, model ELAN 6000 – SCIEX. For isotopic ratio measurement, with the parameters of gas flow of approximately 1.1 L min-1, RF 600 W, gas flow rate 1.2 L min-1, Peak Hopping mode, dwell time 80 and 480 for 6Li and 7Li (respectively), 50 sweeps per reading, 1 read per replicate and 10 replicates. Results: A total of 63 samples were collected from the separation experiment. The Li isotopic ratio measured for each fraction was assessed by ANOVA one-way considering the differences among fractions. A statistical significant difference was observed between the fraction 1 and the remainder fractions and the load solution. The remaining fractions showed an isotopic ratio around the natural abundance (6Li/7Li: 7.59%/92.41% = 0.082). The isotopic ratio of this sample indicated enrichment of 7Li of 0.92% in the fraction number 30. Conclusions: The method of ion exchange with Dowex 50W-x16 resin was efficient on 7Li separation and through ICP-MS method was able to measure the δ 84%₀ enrichment of the 7Li. The isotopic separation procedure via ion exchange is still being studied, however the results are promising.

    Como referenciar este item
    Icon

  • IPEN-DOC 29564

    ANDRADE, MARIANA N. de ; OTOMO, JULIANA I. ; GIMENEZ, MAISE P. ; NASCIMENTO, LETICIA da S. ; NASCIMENTO, HENRIQUE B. do ; LEAO, PAULO H.B. ; CECILIO, PRISCILA de S. ; FERREIRA, JOAO C. ; BERGAMASCHI, VANDERLEI S. ; BUSTILLOS, OSCAR V. . Sodium interference in lithium isotope ratio analysis by Inductively Coupled Plasma Mass Spectrometry. In: SIMIONATO, ANA V.C. (Coord.) IBEROAMERICAN CONFERENCE ON MASS SPECTROMETRY, 3rd, December 10-15, 2022, Rio de Janeiro, RJ. Abstract... Campinas, SP: Sociedade Brasileira de Espectrometria de Massas - BrMASS, 2022. p. 380-381.

    Abstract: Introduction: Naturally occurring lithium consists of two stable isotopes, 6Li (7.591%) and 7Li (92.409%) and have applications in nuclear technology, pharmaceutical, automotive and geological research. Enriched 7Li isotope in LiOH form has been used as a pH regulator for Pressurized Water Reactor (PWR) reducing corrosion in the primary water circuit [1-3]. The determination of lithium isotopic composition was analyzed by Inductively Coupled Plasma Mass Spectrometry after ion exchange processes, which has been considered a promising technique for the separation of Li isotopes. One of the concerns in ICP-MS analysis is sodium interference. The presence of sodium in the lithium-containing sample has potential implications for the accuracy of isotopic ratio measurements. For this reason, a method is described for the study of sodium interference in 7Li 295,88%₀ and 303,30%₀ enriched solutions. Methods: For this study, the reference standard L-SVEC was used, it has an isotopic abundance of 92.409% for 7Li and 7.591% for 6Li (6Li/7Li ratio = 0.08251) and solutions enriched at 295,88%₀ (6Li/7Li ratio = 0.06661) and 303,30%₀ (6Li/7Li ratio = 0.06810) of 7Li. Concentrations of 50 μg L-1 of lithium were maintained for the solutions used and it was evaluated with the addition of 50, 100, 300, 450 and 1000 μg L-1 of sodium on the enriched samples suffered significant changes in their isotopic ratio. The ICP-MS used for the sample analysis was a PerkinElmer SCIEX Elan 6000. For the isotopic ratio measurement the parameters used was nebulizer gas flow of approximately 0.94 L min-1 , Radio Frequency (RF) 600 W, gas flow rate 1.2 L min-1 , Peak Hopping mode, dwell time 80 and 480 ms for 6Li and 7Li respectively, 50 sweeps per reading, 1 read per replicate and 10 replicates. Results: For this study, 24 samples were analyzed being divided into four sets of samples with 6 samples each set. The sets were composed of samples without addition of sodium and samples with addition of 50, 100, 300, 450 and 1000 μg L-1 of sodium. The first set of samples, composed of the 295,88%₀ enriched sample, had a standard deviation of 3.59✕10-4. The second set of samples, composed of the 303,30%₀ enriched sample, had a standard deviation of 2.63✕10-4. The third and fourth set of samples, composed of aliquots of the L-SVEC standard, obtained a standard deviation of 2.22✕10-4 e 2.54✕10-4, respectively. Showing that the addition of sodium did not significantly interfere in the ratio of lithium isotopes 6 and 7 according to standard deviation. Conclusions: Through the results obtained from the experiment, it was observed that the variation in the ratio between isotopes 6 and 7 of lithium was not significant for the results of analysis in ICP-MS. However, it should be noted that for the purposes of lithium isotope separation processes using ion exchange resins, the interference of sodium in the chromatographic separation needs to be evaluated.

    Como referenciar este item
    Icon

  • IPEN-DOC 29563

    TAPPIZ, BRUNO ; SILVA, PAULO S.C. da ; OSTERMANN, CAROLINE ; LIMA, NICOLE P. de ; ANDRADE, MARIANA N. de ; BUSTILLOS, OSCAR V. . Comparison of 238U mass fraction measured by ICP–MS and Instrumental Neutron Activation Analysis techniques. In: SIMIONATO, ANA V.C. (Coord.) IBEROAMERICAN CONFERENCE ON MASS SPECTROMETRY, 3rd, December 10-15, 2022, Rio de Janeiro, RJ. Abstract... Campinas, SP: Sociedade Brasileira de Espectrometria de Massas - BrMASS, 2022. p. 237-238.

    Abstract: Introduction: Uranium is an element present in ambient air, water, soil and rocks [1]. The most abundant natural radioisotope of this actinide (238U) produces in its chain decay the radionuclide 222Rn [2], which is a tracer for atmospheric mixing and transport model validations [3]. The 238U activity concentration in superficial soil samples is a crucial parameter for some 222Rn flux map modeling methodologies [4]. Methods: In this study, 18 superficial soil samples were analyzed. The content of 238U was assessed using two techniques: (i) by the comparative method of the Instrumental Neutron Activation Analysis (INAA) [5] and by ICP–MS (ELAN 6000, PerkinElmer) [6]. Certified Reference Materials (CRM) SRM 1646a Estuarine Sediment and USG STM–2 were used both as comparators in the INAA technique and to ensure quality control. The normality and homoscedasticity of the data were evaluated. Finally, the Mann- Whitney U test [7] was used to assess whether or not there is a significant difference (α = 0.05) between the two techniques. Results: The z-score was calculated to ensure quality control. Z-scores values was obtained by the Modified Horwitz Equation [8], which takes into account the order of magnitude of the content of the analyte and is independent of the CRM uncertainties, unavailable for the CRM used. The z-score values obtained (n = 3) for the radionuclide 238U were 0.5 for the CRM USG STM–2 and -0.4 for SRM 1646a. The z-scores obtained are smaller than 2 therefore they are considered satisfactory [9]. Regarding the statistical parameters of the comparison between the techniques, the normality (Shapiro Wilk) was not verified (p = 0.02 for ICP-MS and p = 0.03 for INAA). The p-value for the test for equal variances (F test) was 0.71 Mann-Whitney U test was used instead of Student’s test due to the non-verification of the normality parameter in the data. The p-value from the Mann-Whitney test (0.51) indicates that there is no significant difference between the 238U content measured by the two techniques. Conclusions: Statistical analysis showed that there is no significant difference between the 238U mass fraction measured by the ICP–MS and the INAA – comparative method. In the future, certified reference materials will also be assessed by ICP–MS in order to corroborate this statistical comparison from a metrological point of view.

    Como referenciar este item
    Icon

  • IPEN-DOC 29562

    ROVELO, BEATRIZ S. ; OSTERMANN, CAROLINE ; WILLIAMS, JONATHAN; TAPPIZ, BRUNO ; ZANNONI, NORA; GUENTHER, ALEX; VEGA, OSCAR . Evaluation of two statements methods by TD-GC-MS/TOF to BVOCs concentrations above the Amazon canopy. In: SIMIONATO, ANA V.C. (Coord.) IBEROAMERICAN CONFERENCE ON MASS SPECTROMETRY, 3rd, December 10-15, 2022, Rio de Janeiro, RJ. Abstract... Campinas, SP: Sociedade Brasileira de Espectrometria de Massas - BrMASS, 2022. p. 235-236.

    Abstract: Introduction: Many biogenic volatile organic compounds (BVOCs) are chiral, meaning they naturally occur as two mirror images of the same molecule. Past and current studies on chiral BVOCs have highlighted the existence of regiospecific patterns [1] and their variability with time of the day, season and height [2]. To better elucidate the role of the tropical forest as a source or a sink of chiral VOC was determined the concentration in two distinctive GC-MS/TOF methods in Amazon rainforest. Methods: Air samples were collected in the Amazon Tall Tower Observatory (ATTO), located 150 km NE of Manaus, Brazil (02°08.752’S, 59°00.335’W) [3]. The site was chosen for having a tower enclosed into the canopy allowing it to measure above the canopy height. Samples were taken at 40m height during July/2019 and 2022 (wet season). The concentration of VOC in each sample was determined through GC-TOF-MS (Markes International, UK) at the Max Planck Institute for Chemistry and at University of California Irvine with the same type of detector. The GC-TOF-MS in Germany [4] is equipped with a thermal desorption unit, a chiral column and a TOF MS which operates in tandem mode and the TD-GC-MS/TOF in the USA is equipped with just one column [5]. Results: Data obtained from the chiral method shows that the concentration of enantiomeric compounds must be different than the simple column method used by the same analyser and mass detector. Conclusions: The second column in addition to TD-GC-MS/TOF can allow the enantiomeric BVOCs identification related to the amount of concentration measured in the Amazon rainforest.

    Como referenciar este item
    Icon

  • IPEN-DOC 29561

    LEBRE, DANIEL T. ; THIPE, VELAPHI C. ; COTRIM, MARYCEL E.B. ; BUSTILLOS, JOSE O.V. . Sugarcane beyond the sweetness: one application in green chemistry. In: SIMIONATO, ANA V.C. (Coord.) IBEROAMERICAN CONFERENCE ON MASS SPECTROMETRY, 3rd, December 10-15, 2022, Rio de Janeiro, RJ. Abstract... Campinas, SP: Sociedade Brasileira de Espectrometria de Massas - BrMASS, 2022. p. 72-73.

    Abstract: Introduction: Sugarcane is a well known source or raw material to produce sugar, alcohol and bioethanol. Brazil is the world’s largest producer of sugarcane (Saccharum sp.), followed by India, China, and Bangladesh, all of which generate significant amounts of bagasse as a by-product of their sugar and bioethanol mills in each of these countries.1 The sugarcane bagasse (SCB) is a fibrous material remaining after the plant's stalk pressing process used to extract the sweet juice used for the industrial production of sugar and alcohol. SCB is one of the most economically viable and readily accessible agro-industrial residues in the world, particularly in tropical regions.2 Recently SCB has taken attention in scientific community, manly in chemistry, because exhibits strong biosorption capabilities, which are defined as the passive sorption of organic and inorganic substances in soluble or insoluble forms from an aqueous solution utilizing decomposing biological materials. Sarker at al. published a comprehensive study of the SCB biosorption properties and their application.3 The purpose of this work is to demonstrate the continued applicability of SCB as SPE biosorbent bed for extracting synthetic hormones (ethynylestradiol, drospirenone and levonogestrel) from pharmaceutical industrial effluent prior LC-MS/MS quantitative analysis. Methods: SPE: SCB 75 μm bed at a concentration of 50 mg was used to pack empty 1 cc (mL) SPE cartridges containing retained frits. To compare the outcomes of the recoveries, the following commercial (cSPE) were used: Oasis HLB 30 mg, 1 mL; Discovery DSC-18 50 mg, 1mL and Sulpeclean Envi-Carb, 100 mg, 1 mL. Conditioning: 1 mL of methanol and 1 mL of water; Load: 1 mL of sample; Wash: 2x 0.75 mL of water; Dry cartridge: under synthetic air flow; Elution: 2x 0.3 mL MeOH. Prior to LC-APCI-MS/MS analysis, the MeOH extracted sample residue was dried in 10 mL class assay tube under N2 gas flow at 40 ºC using and dissolved in 1 mL of 50% acetonitrile/50% water (v/v) solution. Results: The sorption effectiveness of the SCB bed was evaluated using the following experimental parameters in comparison to existing and commercially available SPE (cSPE) cartridges: (i) SCB re-use (recycling); (ii) load concentration variation; (iii) breakthrough (mass/volume capacity); (iv) pH variation; (v) extraction solvents variation (vi) particle size; and (vii) suitability for use in industrial effluent. The SCB SPE results (accuracy data ranged from 99% to 120%) were significantly acceptable for all the synthetic hormones compounds tested and analyzed in industrial effluent samples and comparable to Oasis HLB (benzene copolymer) SPE material and the others. Conclusion: Our overarching objective for developing SCB as SPE sorbent material for the analysis of synthetic hormones from industrial effluents has expanded the research area and/or application of SCB, which the biomass is often burnt to generate energy in the sugar and alcohol mills.

    Como referenciar este item
    Icon

  • IPEN-DOC 29560

    OSTERMANN, CAROLINE ; WILLIAMS, JONATHAN; ZANONNI, NORA; KESSELMEIER, JURGEN; TAPPIZ, BRUNO ; GUENTHER, ALEX; VEGA, OSCAR . Chiral BVOCs composition over the Amazon and Atlantic Rainforest by TD-GCxGC-TOF/MS. In: SIMIONATO, ANA V.C. (Coord.) IBEROAMERICAN CONFERENCE ON MASS SPECTROMETRY, 3rd, December 10-15, 2022, Rio de Janeiro, RJ. Abstract... Campinas, SP: Sociedade Brasileira de Espectrometria de Massas - BrMASS, 2022. p. 64-65.

    Abstract: Introduction: Many BVOCs are chiral, meaning they exist in two non-superimposable mirror image forms termed enantiomers. Enantiomers have identical physical properties, such as boiling point, density, and reactivity to atmospheric oxidants; however, they differ in their specific biological activity. Since most monoterpene measurements do not distinguish enantiomers, relatively little is known about the natural abundance and behavior of the chiral speciated molecules. Previous studies have reported regiospecific patterns across Tropical and Boreal ecosystems for α-pinene [1] and have used them as tracers of secondary processes in air and soil [2][3], highlighting the potential use of chiral compounds in air to decipher processes linking the biosphere with the atmosphere. To better understand the sources, dynamics and sinks of BVOC in the forest environment we have investigated chiral BVOC in the pristine Amazonian rainforest environment at Amazon Tall Tower Observatory (ATTO) is located 150 km from Manaus/BR and in Atlantic Forest located in the Ecological Immigrant Park (PEI) at São Paulo/BR in 2019. Methods: Samples collected in 2019, were thermally desorbed, separated and analyzed through a Bench-TOF select (TD-GCxGC-TOF/MS tandem ionization-14eV and -70eV) from Markes International, UK. First, all samples through to Thermo-Desorption, that one we separated in two desorption: Primary one we use at the cartridges during 10 min in 250°C and in the second one is for trap during 10 min with 250°C. In the GC the column is Dimethyl TBS Cyclodextrin based so the oven was settled in 5 min at 40°C and 1.5°C/min during the temperature increase from 40°C until 150°C and then 30°C/min from 150°C until 200°C. Results: The VOCs and their chirals analyzed are: (-/+) α- Pinene, (-/+) Camphene, (-/+) β- Pinene, (-/+) Limonene, these chiral are of extreme importance because the fact of obtaining the same chemical composition, but different geometries the (-) and (+) differ in nature in their function/aroma, for example the ( -) α- Pinene is found in pine and (+) α- Pinene is found in eucalyptus, such as (-) Limonene emitted by orange and (+) Limonene emitted by lemon. The separation method was specifically designed for the separation of chiral monoterpenes (C H ) and sesquiterpenes (C H ). A chiral column is therefore needed to separate the two enantiomers, standard columns for GC-MS, comparing to an online PTR-MS would not allow enantiomeric separation. Conclusions: It was concluded that the ratio between the pairs of enantiomeric concentrations show a temporal and spatial variability. In forests not disturbed by humans, was found a significant amount of (-) α-Pinene, and due to human interference located in the metropolitan region of São Paulo, (+) Limonene was the BVOC with the highest concentration.

    Como referenciar este item
    Icon

  • IPEN-DOC 29550

    VASQUEZ, P.A.S. ; NAGAI, M.L.E. ; OLIVEIRA, M.J.A. ; OTUBO, L. ; SOMESSARI, E.S.R. . Preservation of photographic and cinematographic films by electron-beam irradiation. In: INTERNATIONAL CONFERENCE ON ACCELERATORS FOR RESEARCH AND SUSTAINABLE DEVELOPMENT, May 23-27, 2022, Vienna, Austria. Abstract... Vienna, Austria: International Atomic Energy Agency - IAEA, 2022.

    Abstract: The Nuclear and Energy Research Institute – IPEN through the Multipurpose Gamma Irradiation Facility and the Electron Beam Irradiation Facilities has disinfected several tangible cultural collections from the University of São Paulo – USP. Brazilian weather conditions added to the actions of insects and fungi promote biodegradation especially in cellulose-based materials. In this sense, ionizing radiation is an excellent alternative to the traditional preservation process mainly because the biocidal action. Electron beam irradiation also presents new possibilities for processing materials with greater speed, despite having limited penetration. Adequate storage of photographic and cinematographic materials is a challenge for experts from preservation institutions. Contamination by fungi is one of leading causes of problem in this kind of collections. In addition, another common physicochemical degradation affecting cellulose triacetate films causing deacetylation of polymer chain is called “vinegar syndrome”. In this work are presented results of the effect of the electron beam irradiation on photographic and cinematographic films using an electron beam accelerator with energy of 1.5 MeV and beam power of 37.5 kW. Selected film samples were characterized by FTIR-ATR spectroscopy and FEGSEM-EDS microscopy. Samples were irradiated with absorbed dose between 2 kGy and 200 kGy. Irradiated samples were analyzed by UV-Vis spectrophotometry, FEGSEM, thermogravimetric analysis (TG) and differential scanning calorimetry (DSC). Results showed that disinfection by electron beam radiation can be achieved safely applying radiation absorbed doses between 6 kGy to 10 kGy with no significant change or modification of main properties of the constitutive polymeric materials. Electron beam irradiation, due to the effect of crosslinking is presented as an alternative to treat films affected by “vinegar syndrome” applying absorbed dose of 80 kGy in order to increase shelf life of cultural heritage materials.

    Como referenciar este item
    Icon

  • IPEN-DOC 29549

    CALVO, W.A.P. ; MUNHOZ, P.M. ; SOMESSARI, S.L. ; DUARTE, C.L. ; SPRENGER, F.E. ; FEHER, A. ; LAINETTI, F.F. ; GASPAR, R.R. ; NASCIMENTO, F.C. ; SILVA, L.G.A. ; HARADA, J. ; BRAGA, A. ; RODRIGUES, M.; SAMPA, M.H.O. . Electron beam processing to improve biodegradable polymers and for industrial wastewater treatment and recycling. In: INTERNATIONAL CONFERENCE ON ACCELERATORS FOR RESEARCH AND SUSTAINABLE DEVELOPMENT, May 23-27, 2022, Vienna, Austria. Abstract... Vienna, Austria: International Atomic Energy Agency - IAEA, 2022.

    Abstract: Radiation technology has been used to control environmental pollution. The aim of these studies was to apply the electron beam radiation technology for controlling plastic pollution and environmental protection.

    Como referenciar este item
    Icon

  • IPEN-DOC 29548

    KOTANI, PALOMA O. ; FERRO, DAPHNE M. ; TAVARES, LAURA P. ; AFFONSO, REGINA ; ORTIZ, NILCE . Using TiO2-Diatomite for photodisinfection in contaminated wastewater. In: ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY (SBBq), 51st; CONGRESS OF BRAZILIAN BIOPHYSICAL SOCIETY (SBBf)/LATIN AMERICAN FEDERATION OF BIOPHYSICAL SOCIETIES (Lafebs), 46th, September 5-8, 2022, Águas de Lindóia, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq, 2022. p. 212-212.

    Abstract: INTRODUCTION: The urban pressure reduced water availability and quality through the years, promoting the development of water treatment and disinfection processes such as the Advanced Oxidation Process (AOP). A highly efficient catalyst with Diatomite as biotemplate and solar energy can enhance hydroxyl radicals (OH) production for disinfection and pollutants degradation. Preliminary TiO2-Diatomite experiments with Escherichia coli provided valuable insights on its photodisinfection efficiency, legitimating its usage in the wastewater samples presented in this study. OBJECTIVES: Evaluate the use of TiO2-Diatomite in photodisinfection process in contaminated wastewater. MATERIALS AND METHODS: TiO2 synthesis used titanium isopropoxide sol-gel process with Diatomite powder, the filtration step followed the mixed water suspension, and the drying process lasted overnight. The wastewater samples were collected from a household washing machine and 0.05 g of TiO2-Diatomite were added in the photodisinfection reactor. The total reaction lasted for 90 minutes in the solar chamber with all parameters controlled. The suspension aliquots were collected after 30 minutes of agitation and plated on LB agar at Petri plates. After incubation, the emerged colonies were counted through software (OpenCFU) and the data processed using R programming language. DISCUSSION AND RESULTS: The Scanning Electron Microscopy (SEM) micrograph of TiO2-Diatomite presented enhanced surface area and microstructure obtained by biotemplate addition. The bacterial inactivation percentage was above 75 % for 1 hour of solar radiation exposure. Kinetics models indicated better correspondence with interparticle reaction. CONCLUSION: Photodisinfection kinetics studies provided more efficient bacterial inactivation with the addition of 0.05 g of TiO2-Diatomite in the sample. The study presents an affordable and sustainable treatment using a viable renewable energy source for application in distant areas with contaminated effluents with the addition of a reagent easily obtained by government agencies.

    Como referenciar este item
    Icon

  • IPEN-DOC 29547

    RAPOSO, BRUNO L.; SOUZA, SUEDEN O.; SANTANA, GLEYCIANE S. de; SARMENTO-NETO, JOSE F.; SANTOS, BEATE S.; RIBEIRO, MARTHA S. ; REBOUCAS, JULIO S.; CABRAL FILHO, PAULO E.; FONTES, ADRIANA. Biophotonic strategy associated with hexyl zinc porphyrin for inactivation of Candida spp.. In: ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY (SBBq), 51st; CONGRESS OF BRAZILIAN BIOPHYSICAL SOCIETY (SBBf)/LATIN AMERICAN FEDERATION OF BIOPHYSICAL SOCIETIES (Lafebs), 46th, September 5-8, 2022, Águas de Lindóia, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq, 2022. p. 271-271.

    Abstract: INTRODUCTION: The genus Candida is among the most frequent fungal pathogens worldwide. The indiscriminate use of antifungals enables the spread of resistant strains, which have been associated with high morbidity and mortality. Photodynamic inactivation (PDI) is a promising technology to treat resistant Candida spp. infections. PDI occurs when light excites a photosensitizer (PS) leading to the production of reactive oxygen species (ROS). Zn(II) porphyrins (ZnPs) present high efficiency for intracellular ROS generation and structural versatility for tailored lipophilicity and ionic character, modulating the bioavailability and interaction with cellular structures. OBJECTIVES: This study aimed to investigate the potential of ZnTnHex-2-PyP4+-mediated PDI to inactivate C. albicans and C. glabrata yeasts. MATERIALS AND METHODS: Candida yeasts (1×10^7 CFU/mL) were evaluated according to the groups: (i) control (without treatment); (ii) only ZnTnHex-2-PyP4+ (dark); (iii) only light (blue LED); and (iv) PDI (ZnP + light) using 10 min of pre-incubation. Different ZnP concentrations (0.15 to 1.25 μM) and light doses were firstly tested with C. albicans. Treated samples were diluted and seeded on Sabouraud agar for colony enumeration after incubation at 37 ºC for 24 h. DISCUSSION AND RESULTS: C. albicans viability decreased with increasing ZnP concentration, achieving complete eradication at 0.8 μM and 3 min of irradiation (24.1 mW/cm²). PDI with 1.25 μM and 1 min of irradiation resulted in a 2 log10 reduction only, demonstrating the importance of light dose in microbial photoinactivation. PDI parameters were subsequently adjusted for inactivation of C. glabrata. Complete C. glabrata eradication was achieved with ZnP at 0.8 μM, and 3 min of irradiation, however, at a higher irradiance (38.4 mW/cm²). Groups treated with either light or ZnP alone did not affect Candida spp. viability. CONCLUSION: These results suggest that the protocols used in this study were efficient for inactivating Candida spp. yeasts at sub-micromolar concentration ZnP and short irradiation times.

    Como referenciar este item
    Icon

  • IPEN-DOC 29546

    SILVA, CAMILA R. ; PEREIRA, SAULO de T. ; PRETTO, LUCAS R. de ; FREITAS, ANDERSON Z. de ; RIBEIRO, MARTHA S. . Photobiomodulation therapy as a radiosensitizer for triple-negative breast cancer. In: ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY (SBBq), 51st; CONGRESS OF BRAZILIAN BIOPHYSICAL SOCIETY (SBBf)/LATIN AMERICAN FEDERATION OF BIOPHYSICAL SOCIETIES (Lafebs), 46th, September 5-8, 2022, Águas de Lindóia, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq, 2022. p. 273-273.

    Abstract: INTRODUCTION: Radiotherapy (RT) is an essential cancer treatment and is estimated that approximately 52% of oncological patients will be submitted to this technique once. However, some tumors, such as triple-negative breast cancer (TNBC), present radioresistance, demanding high doses of ionizing radiation (IR) and a prolonged period of treatment, which contributes to secondary malignancies due to deposition of dose in organs at risk and several side effects. Moreover, this subtype of cancer shows a high incidence of metastasis and decreases the survival expectancy of the patient. Thus, the search for new agents that can act as a radiosensitizer to improve the RT effects has been growing. Conversely, photobiomodulation therapy (PBM), which is a promising therapy with increasing adhesion in clinical practice, has been used to mitigate the adverse effects of RT. Indeed, recent studies have associated PBM with RT to combat cancer. OBJECTIVES: In this study, we used TNBC-bearing mice as a radioresistant cancer model to verify if PBM could act as a radiosensitizer MATERIALS AND METHODS: PBM was applied in two different protocols before the RT with a high dose (60 Gy fractioned in 4 sessions). We evaluated the tumor volume progression, animal clinical evolution, lung metastases by optical coherence tomography, and animal survival DISCUSSION AND RESULTS: Our data indicate that PBM before each RT session arrested the tumor volume, improved the clinical signals of the animals, reduced the nodules in the lung, and extended animal survival. CONCLUSION: In the light of the knowledge gained, our data indicate that PBM could act as a radiosensitizer.

    Como referenciar este item
    Icon

  • IPEN-DOC 29545

    PRATA, BEATRIZ A. ; SANTOS, CAROLINA M. dos ; AFFONSO, REGINA . Optimization of the process of expression in E. coli and purification of the catalytic sites of the ACE1 by the ELP-Intein system. In: ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY (SBBq), 51st; CONGRESS OF BRAZILIAN BIOPHYSICAL SOCIETY (SBBf)/LATIN AMERICAN FEDERATION OF BIOPHYSICAL SOCIETIES (Lafebs), 46th, September 5-8, 2022, Águas de Lindóia, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq, 2022. p. 220-220.

    Abstract: INTRODUCTION: Angiotensin I-converting enzyme (ACE) is a fundamental part of the renin-angiotensin system; this has two domains, N- and C-, each of which has a catalytic site that exhibits 60% sequence identity. Its actions are in the control of blood pressure, protection of the brain by cleavage of beta-amyloid bodies, cell proliferation, formation of hematopoietic stem cells, among others. OBJECTIVES: Obtaining the catalytic sites Ala361 to Gly468 (N domain region, csACEN) and Ala959 to Ser1066 (C domain region, csACEC) in pure form and with their correct structural conformation. MATERIALS AND METHODS: Expression conditions of pE1csACEN and pE1csACEC vectors in E. coli BL21(DE3) strain: cultures grown in Terrific Broth at 37⁰C at 140 rpm for 20–24 h and 0.1 mM IPTG. Purification by Elastin-like Polypeptide (ELP) precipitation: ELP-bound catalytic sites were purified with two ammonium sulfate precipitations (ASp). Remotion of ELP: by autocleavage of the Intein sequence using the buffers: sodium phosphate, sodium cacodylate, MES and Tris-HCl. The ELP/Intein was removed from the sample by ASp. The analyzes of all stages of the process were performed by SDS-PAGE and Dot blotting. DISCUSSION AND RESULTS: The differential for obtaining the pure peptides was the temperature of 37⁰C, with a significant increase in expression concerning the cultivation of 16⁰C. In the ELP purification steps, ammonium sulfate buffer concentrations of 0.57 M and 0.8 M were the most efficient. Intein's self-cleaving was more efficient with MES buffers and Tris-HCl for ELPsACEN and ELPsACEC, respectively. Structural analysis by Circular Dichroism and Fluorescence confirmed the correct structure of the pure peptides. CONCLUSION: In the present work, we defined the most efficient conditions for expression, purification, and obtaining of ACE catalytic sites in pure form. The csACEN and csACEC peptides will allow greater assertiveness in obtaining and characterizing new hypertensive drugs and in the hydrolysis of substrates such as beta-amyloid.

    Como referenciar este item
    Icon

  • IPEN-DOC 29544

    CABRAL, FERNANDA V. ; SMITH, TERRY K.; RIBEIRO, MARTHA S. . Lipidomics analysis of Leishmania amazonensis following photoxidative stress. In: ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY (SBBq), 51st; CONGRESS OF BRAZILIAN BIOPHYSICAL SOCIETY (SBBf)/LATIN AMERICAN FEDERATION OF BIOPHYSICAL SOCIETIES (Lafebs), 46th, September 5-8, 2022, Águas de Lindóia, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq, 2022. p. 272-272.

    Abstract: INTRODUCTION: Antimicrobial photodynamic therapy (APDT) is a well-known light-based technology that has been widely studied as an alternative approach to fight cutaneous leishmaniasis (CL). APDT induces lipid peroxidation in cellular membranes due to the generation of oxidative stress OBJECTIVES: In this study, we evaluated the role of 1,9- dimethylmethylene blue (DMMB)-mediated APDT on a wild-type (WT) and a miltefosine-resistant (MF) strain of Leishmania amazonesis and analyzed several cellular processes to get insights into the underlying mechanisms of APDT. MATERIALS AND METHODS: For this, APDT was carried out using red light (λ= 67012 nm) and promastigotes were exposed to different concentrations of DMMB at 8 J/cm2. Then, we measured mitochondrial potential and intracellular levels of reactive oxygen species (ROS) and analyzed quantitative lipidomics of the main phospholipid classes using electrospray-mass spectrometry. DISCUSSION AND RESULTS: As a result, we observed overproduction of ROS, mitochondrial membrane depolarization, and a rapid lipid remodeling immediately after APDT. Of note, MF showed a higher content in levels of phosphatidylcholine (PC) as compared to the WT line before treatment, which suggests it could be also involved in the MF resistance mechanism. In addition, results showed that after APDT, PC levels were substantially decreased, while new phospholipid species of phosphatidylethanolamine (PE) were increased. CONCLUSION: In conclusion, our data suggest DMMB-mediated APDT promoted a significant lipid peroxidation in the parasite's membrane of both strains, which failed to manage redox imbalance, thus resulting in cellular malfunction and death.

    Como referenciar este item
    Icon

  • IPEN-DOC 29543

    SEPULVEDA, ANDERSON F.; VOLLRATH-KUMPGDEE, MONT; FRANCO, MARGARETH K.K.D. ; YOKAICHIYA, FABIANO; ARAUJO, DANIELE R. de. Hyaluronic acid incorporation modulates rheological and drug release properties in Poloxamer-based hydrogels. In: ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY (SBBq), 51st; CONGRESS OF BRAZILIAN BIOPHYSICAL SOCIETY (SBBf)/LATIN AMERICAN FEDERATION OF BIOPHYSICAL SOCIETIES (Lafebs), 46th, September 5-8, 2022, Águas de Lindóia, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq, 2022. p. 254-254.

    Abstract: INTRODUCTION: Synthetic polymer Poloxamer (PL) 407 (15% and 30% w/w) and binary formulation PL 407 15% + PL 338 15% (BF), with natural polymer hyaluronic acid 0.5% w/w, were designed as bupivacaine or ropivacaine thermosensitive release systems. OBJECTIVES: The aim of this work is to characterize structure and stability of drug delivery systems. MATERIALS AND METHODS: These systems were characterized by calorimetry, rheology, SANS, and release profile. DISCUSSION AND RESULTS: Calorimetry results demonstrated all formulations are stable at storage and physiological temperatures. PL 407 30% and BF systems are structurally more organized and with higher consistency (G’/G’’ ~ 50) at 37 °C and with lower gelation temperature (Tg ~ 14 °C) than PL 407 15% ones (G’/G’’ ~ 0.30 and Tg ~ 45 °C, respectively), however BFs have increased viscosity and slightly higher stiffness (G’/G’’ ~ 60) when compared to PL 407 30% formulations, due to more hydrophilicity of PL 338 chains than PL 407. Adding HA, it is observed enhanced viscosity but diminished consistency (G’/G’’ ~ 0.40). When a drug is incorporated, it is seen that it promotes increased interaction between chains. Although material alteration when incorporating HA or drug is observed, SANS results showed that the type of supramolecular structure is dependent on the concentration of Poloxamer. Systems with low concentration of Poloxamer have lamellar type, while formulations with 30% of Poloxamer have both cubic and hexagonal structures. In addition, PL 407 30% formulations undergo greater compression when bupivacaine is added (~ 29.7 nm at 25 °C and 37 °C). As drug release profiles showed, BFs release drugs in a more controlled way than other formulations. Moreover, HA hinders the release of both drugs. CONCLUSION: Thus, it is clear that the incorporation of more hydrophilic polymers is able to modulate the drug release rate according to the hydrogels rheological parameters.

    Como referenciar este item
    Icon

  • IPEN-DOC 29542

    OLIVEIRA, SORAIA B. de ; FERREIRA, LUANA da S. ; MARUMO, MARIA H.B. . Expression profile of zinc channels in human renal cell carcinoma after Temsirolimus treatment. In: ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY (SBBq), 51st; CONGRESS OF BRAZILIAN BIOPHYSICAL SOCIETY (SBBf)/LATIN AMERICAN FEDERATION OF BIOPHYSICAL SOCIETIES (Lafebs), 46th, September 5-8, 2022, Águas de Lindóia, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq, 2022. p. 364-364.

    Abstract: INTRODUCTION: Zinc is an essential trace element for cell proliferation and growth. Cellular Zn is regulated of by ZnT and ZIP family channels but its mechanism still not completely understood. Renal cell carcinoma (RCC) is one of the most malignant renal tumors. The RCC clear cell pathological subtype is associated with the VHL gene mutation, that is responsible for its aggressiveness. Temsirolimus (TEM), an antineoplastic drug used in the treatment of RCC, is a selective inhibitor of mTOR. OBJECTIVES: To evaluate the expression of zinc channels in clear cell renal carcinoma cell line with and without TEM treatment. MATERIALS AND METHODS: The MTS (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium-bromide) assay was used define IC50. The expression of ZnT1, ZnT4, ZIP11 and ZIP14 channels from the HK-2, 786-0 and 786-0/TEM cells was evaluated by real-time PCR and Western blot analysis. DISCUSSION AND RESULTS: The IC50 dose was 10μM. Gene expression analysis comparing Hk-2 and 786-0 cell lines revealed decreased levels of ZnT1 of 79.20±3.58% (P< 0.0001 vs HK-2) and an increase for the ZIP 11 of 243.3±62.84% (P< 0.01 vs HK-2). The comparison between 786-0 with and without TEM treatment showed decreased levels of ZnT1 of 34.03±20.45% (P< 0.05 vs 786-0), ZnT4 of 92.82±0.72% (P< 0.0001vs 786-0), ZIP14 of 11.24% (P< 0.01 vs 786-0) and ZIP11 of 95.96±0.54% (P< 0.0001 vs 786-0). Western blot data corroborated the real time results. CONCLUSION: There is a difference in the Zn channel expression profiles between HK-2 and 786-0. The treatment with TEM modulates the expression of these channels.

    Como referenciar este item
    Icon

  • IPEN-DOC 29541

    TEIXEIRA, LUIZ F.S. ; BELLINI, MARIA H. . Evaluation of angiogenic capacity of human adenocarcinoma cell line knockout for NF-ĸΒ1 protein. In: ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY (SBBq), 51st; CONGRESS OF BRAZILIAN BIOPHYSICAL SOCIETY (SBBf)/LATIN AMERICAN FEDERATION OF BIOPHYSICAL SOCIETIES (Lafebs), 46th, September 5-8, 2022, Águas de Lindóia, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq, 2022. p. 415-415.

    Abstract: INTRODUCTION: Renal cell carcinoma (RCC) is the most common adult renal epithelial cancer. The most frequent subtype of RCC is clear cell (ccRCC). Most of ccRCC patients have a mutation in the Von Hippel-Lindau (VHL) tumor suppressor gene. The VHL gene encodes a protein, the VHL, which can up-regulate a series of intracellular proteins, including the hypoxia inducible factor (HIF). The transcription factor NF-кB is increased in the ccRCC. OBJECTIVES: To evaluate the impact of the NF-кB1 gene knockout on the VEGF and IL6 expression in the human RCC cells under normoxia and hypoxia. MATERIALS AND METHODS: The CRISPR/Cas-9 technique was used to obtain 786-0 cells knockout for the NF-кB1 protein. Western Blot assay was used to selected the clones. A hypoxia-inducing humid chamber was used and its effectiveness was validated its effectiveness was certified by the analysis of HIF-2α expression levels. The quantification of VEGF and IL-6 levels was measured using Real Time-PCR and MILLIPLEX assay. DISCUSSION AND RESULTS: The VEGF gene expression in the clones was significantly lower than that presented by the control both in normoxia (786-0-sg1 99.68±0.09%, 786-0-sg2 78.55±0.85%, 786-0-sg3 91.70±0.87%) and in hypoxia (786-0-sg1 98.30±1.49%, 786-0-sg2 75.21±4.14%, 786-0-sg3 98.44±0.18%). The expression of IL-6 gene was also significant lower in normoxia (786-0-sg1 49.03±0.80%, 786-0-sg2 76.59±12.43%, 786-0-sg3 66.98±10.89%) and in hypoxia (786-0-sg1 95.85±0.36%, 786-0-sg2 96.45±0.49%, 786-0-sg3 91.08±1.42%). The MILLIPLEX results show that there was a significant reduction of both VEGF and IL-6 in the culture medium of cells knocked out in normoxia and hypoxia compared to control group. CONCLUSION: Suppression of p50 expression in the clones resulted in the reduction of VEGF and IL6 in both conditions. The reduction in the IL-6 relative expression hypoxia/normoxia demonstrates a change in cellular responsiveness to decreased levels of oxygen.

    Como referenciar este item
    Icon

  • IPEN-DOC 29540

    FERREIRA, LUANA da S. ; OLIVEIRA, SORAIA B. de ; BELLINI, MARIA H. . Effect of the hypoxia on the expression of zinc transporters in renal adenocarcinoma cell line. In: ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY (SBBq), 51st; CONGRESS OF BRAZILIAN BIOPHYSICAL SOCIETY (SBBf)/LATIN AMERICAN FEDERATION OF BIOPHYSICAL SOCIETIES (Lafebs), 46th, September 5-8, 2022, Águas de Lindóia, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq, 2022. p. 366-366.

    Abstract: INTRODUCTION: Renal cell carcinoma (RCC) is a tumor responsible for about 1 to 3 % of all malignancies. The most common histological variant is the clear cell carcinoma (ccRCC), representing about 45% of all cases of RCC in adults. ccRCC is associated with the VHL gene mutation. The loss of the VHL protein prevents the degradation of HIF subunits, which are involved in critical oncogenic pathways. Zinc is an essential trace element and its cellular homeostasis is regulated by zinc transporters such as ZIPs and ZNTs. The profile of their expression in renal tumor is still unknown. OBJECTIVES: Evaluate the expression profile of zinc transporters in ccRCC in normoxia and hypoxia culturing conditions. MATERIALS AND METHODS: 786-0 tumor cells were cultured in hypoxic conditions inside a hypoxia chamber with an oxygen absorber to the atmosphere of 1% O2, 5% CO2, and 94% N2, and placed in an incubator at 37°C for 24 hours. The Altair PRO Single-Gas Detector was used to measure the percentage of O2. For gene expression analysis, RTq-PCR was used and the results were analyzed by the Delta-Delta ct method. DISCUSSION AND RESULTS: VEGF and HIF2a expression in 786-0 cells were evaluated to confirm the efficacy of hypoxia chamber. There was a significant increase in the VEGF expression of 312.8±2.14% (P< 0.0001) and HIF2a of 593.4±57.21% (P< 0.0092). Besides that, the gene expression analysis revealed a downregulation in the hypoxic environment of the channels ZNT9 of 71.41±0.84% (P< 0.0001), ZIP1 of 17.45±3.68% (P< 0.0418), ZIP4 of 76.3±9.75% (P< 0.0054) and ZIP10 of 44.96±4.31% (P< 0.0001). CONCLUSION: The hypoxia modulates the expression of Zn channels in 786-0 cells indicating that such channels play a role in the pathophysiology of ccRCC.

    Como referenciar este item
    Icon

  • IPEN-DOC 29539

    SALVEGO, CAMILA de A. ; CABRAL, FERNANDA V. ; SEABRA, AMEDEA B.; SANTANA, BIANCA de M.; RIBEIRO, MARTHA S. . Cytotoxic effects of chitosan nanoparticles containing S-nitrosoglutathione in triple-negative breast cancer cells. In: ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY (SBBq), 51st; CONGRESS OF BRAZILIAN BIOPHYSICAL SOCIETY (SBBf)/LATIN AMERICAN FEDERATION OF BIOPHYSICAL SOCIETIES (Lafebs), 46th, September 5-8, 2022, Águas de Lindóia, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq, 2022. p. 237-237.

    Abstract: INTRODUCTION: Breast cancer is the most common type of cancer affecting women worldwide. Among the treatments, radiation therapy (RT) is frequently chosen as a primary strategy; however, it demands high doses of ionizing radiation to achieve a curative dose. To enhance RT effectiveness, an external agent can be used to sensitize cells before the treatment, allowing a dose reduction. Nitric oxide (NO) is an essential molecule linked to several organic processes, besides being described as a potential radiosensitizer of tumor cells by different mechanisms, including oxidative stress. However, NO have a short half-life in biological conditions, making it difficult to achieve anticancer effects. To overcome this, NO donors can be encapsulated into polymer-based nanoparticles, ensuring a sustained NO releasing. OBJECTIVES: To evaluate the cytotoxicity induced by chitosan nanoparticles containing S-nitrosoglutathione (GSNO-CS NPs) in 4T1 cells (murine triple-negative breast cancer). MATERIALS AND METHODS: Cells were cultivated, seeded in 96-well plates (2 x 10 4 cells/well), and incubated at 37ºC with 5% of CO2 for 24 h. Both CS NPs and CS NPs containing GSNO encapsulated were added to the plates at different concentrations (0-2.4 mg/ml CS NPs, 0-6 mM GSNO) and incubated for 24 h. Cytotoxic effects were evaluated through Resazurin fluorometric assay in both groups. DISCUSSION AND RESULTS: Our results showed a 65% reduction in cell viability for GSNO-CS NPs groups treated at 6 mM, while only 30% of cells were killed when treated by CS NPs group. CONCLUSION: Our data suggest that GSNO-CS NPs were able to promote cytotoxicity effects, thus inducing oxidative stress in triple-negative breast cancer cells. Next steps involve the use of these nanoparticles before RT to evaluate its radiosensitizer effect.

    Como referenciar este item
    Icon

  • IPEN-DOC 29538

    CABRAL, FERNANDA ; RIBEIRO, MARTHA S. . Photodynamic therapy towards inactivation of miltefosine-resistant Leishmania amazonensis. In: ANNUAL MEETING OF THE BRAZILIAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY (SBBq), 51st; CONGRESS OF BRAZILIAN BIOPHYSICAL SOCIETY (SBBf)/LATIN AMERICAN FEDERATION OF BIOPHYSICAL SOCIETIES (Lafebs), 46th, September 5-8, 2022, Águas de Lindóia, SP. Abstract... São Paulo, SP: Sociedade Brasileira de Bioquímica e Biologia Molecular - SBBq, 2022. p. 271-271.

    Abstract: INTRODUCTION: Cutaneous leishmaniasis (CL) is a chronic disease developed by Leishmania parasites that promotes destructive lesions. The emergence of drug-resistant parasites has been related to the misuse of drugs, being a major threat to global health. Although antimicrobial photodynamic therapy (APDT) has been reported as an attractive treatment against a broad spectrum of drug-resistant pathogens, the use of APDT against drug-resistant Leishmania parasites has never been explored. OBJECTIVES: This study aimed to explore the effects of methylene blue-mediated APDT (MB-APDT) on promastigotes and intracellular amastigotes of two different strains of Leishmania amazonensis, a wild-type (WT) and a miltefosine-resistant cell line (MFR). MATERIALS AND METHODS: Promastigotes and intracellular amastigotes were treated at different concentrations of miltefosine. Regarding APDT, we used a red LED (λ= 660±22 nm) at 20 mW/cm 2 and two MB concentrations. Parasites were exposed to radiant exposures of 0 to 25 J/cm 2 .DISCUSSION AND RESULTS: The miltefosine concentration necessary to reduce 50% (EC50) MFR promastigotes was found to be 5.6-fold higher than that of the WT strain. Amastigotes were even more resistant, and the concentration needed to effectively kill MFR was not able to be calculated once it was toxic to health macrophages. Differently, both promastigotes and intracellular amastigotes were susceptible to MB-APDT. Indeed, promastigotes were equally susceptible to treatment regardless of the MB concentration. EC50 calculated for the light dose delivered was nearly 3 J/cm2, which corresponds to an exposure time of 150 s. Surprisingly, amastigotes of MFR were more susceptible to MB-APDT at 50 μM MB concentration, and the light dose necessary to reduce 50% of resistant parasites was half of that of the WT strain (2.3 J/cm 2 and 4.7 J/cm 2 , respectively). CONCLUSION: These results indicate that MB-APDT could be a promising treatment to overcome the global issue of antileishmanial drug resistance in CL.

    Como referenciar este item
    Icon

  • IPEN-DOC 29537

    FUNGARO, DENISE A. ; IZIDORO, JULIANA de C. . Circular economy in the sugarcane agroindustry: bagasse-fly-ash-based zeolite. In: PLANETARY HEALTH ANNUAL MEETING, October 31 - November 2, 2022, Boston, MA, USA. Abstract... Boston, MA, USA: Planetary Health Alliance, 2022. p. 129-130.

    Abstract: The sugarcane industry in the process of producing sugar and ethanol generates bagasse in large amounts. The burning of bagasse used for steam and electricity generation produces 3 million tonnes of bagasse fly ash (BFA) annually. Handling and improper disposal of BFA cause pollution in soils, air, and water, which results in environmental challenges and human health problems. The valorization of BFA is a convenient and sustainable means to reduce solid waste generation and decrease the environmental pollution load. The purpose of this research is to investigate the application of BFA for the synthesis of zeolite. Because of three-dimensional structure and unique porous properties, zeolite is a value-added product with many applications such as adsorbent material and industrial catalysis. After separation of the coarser particles containing mainly unburned carbon, fine particles of BFA were used to synthesize zeolite by alkali fusion (550 oC; 1 h; BFA: NaOH= 1:1.2 w/w) followed by hydrothermal treatment at 100 °C for 3 h. Si/Al molar ratio of 1.0 was adjusted with an external alumina source. BFA and synthesized zeolite were characterized using XFR, XRD, and MEV. The results showed that BFA may be used effectively for the synthesis of zeolite NaA with high purity comparable with the commercial product. Zeolite NaA is employed in sugarcane mills in the dehydration processes for the removal of water from the hydrated ethanol. Thus, the application of BFA in zeolitization process strategy provides not only environmental and economic benefits for the sugarcane agroindustry but also contributes to a circular economy and towards the achievement of Sustainable Development Goal 12.

    Como referenciar este item
    Icon

  • IPEN-DOC 29536

    SANTIAGO, RAQUEL; VIANNA, DANIELA; FUNGARO, DENISE A. ; VIANNA, NELZAIR; SARAIVA, ANTONIO M.. Brazilian Campus Ambassador Program as an educational strategy on Planetary Health. In: PLANETARY HEALTH ANNUAL MEETING, October 31 - November 2, 2022, Boston, MA, USA. Abstract... Boston, MA, USA: Planetary Health Alliance, 2022. p. 34-34.

    Abstract: The Brazilian Campus Ambassadors Program (BCAP) of Planetary Health Brazil has the mission of formally recognizing the leaders of the Next Generation in Planetary Health (PH). Throughout the ten months of the program, under the mentoring of professors and researchers, the ambassadors build their PH network and develop organizational and leadership skills. Along the BCAP, the ambassadors acquire skills and tools to take a leading role in their place of action, educate their community, and facilitate collaboration among the existing fields and initiatives on human health and climate change. The target public are undergraduate and graduate students, who acted as multipliers to involve the academic community and the civil society. This way, the BCAP, which is currently in its second edition, works as a strategy to spread the concept of PH in the Brazilian academic environment, giving it a greater capillarity to be inserted in the different areas of education. In 2021, the first edition of the program, we had 249 ambassadors, accompanied by 33 mentors, and 90 of them managed to finish the activities. For 2022, we opted for a smaller intake: 87 ambassadors, accompanied by 23 mentors - to provide more focused mentoring. The result of the 2021 edition was the union of ambassadors from different areas of knowledge in the realization of lectures, online events and lives, workshops, and active participation in the organization and Poster Sessions of PHAM 2021. They learned the value of working together and communicating in an accessible language. At the same time, they learned that we all have our role in PH, broadening their initial vision on the theme. It is worth highlighting the quantity and quality of the ambassadors' activities, even with the limitations imposed COVID-19 pandemic, even generating the insertion of SP content in undergraduate and graduate courses at Brazilian universities.

    Como referenciar este item
    Icon

  • IPEN-DOC 29535

    MONTEDO, UIARA B.; SANTOS, BARBARA J. dos . Food security and urban gardens, the case of São Paulo/Brazil. In: PLANETARY HEALTH ANNUAL MEETING, October 31 - November 2, 2022, Boston, MA, USA. Abstract... Boston, MA, USA: Planetary Health Alliance, 2022. p. 12-12.

    Abstract: With Covid-19 pandemic, food insecurity in Brazil has become urgent. Since 2020, there has been an increase in unemployment and the closing of schools and day care centers, demanding joint action from society, university, government and companies. Urban and agroecological gardens were highlighted in community actions in the periphery of São Paulo, which has 106 vegetable gardens mapped by the Sampa+Rural program. These gardens are run by solidarity enterprises, which have promoted the delivery of food baskets to vulnerable families. The objective was to enhance the quality of production and ensure healthy food for about 80 families, served by 8 gardens, which contributed to generating income and thus maintaining their dynamics. The methodology consisted of a training cycle from August/21 to April/22, with 5 meetings in each of the 8 participating gardens, covering the planning stages of planting, production, harvest and distribution/marketing. In total, more than 1000 seedlings were planted, ranging from conventional ones (lettuce, arugula, kale, parsley and chives) to seedlings of unconventional food plants, which show a high nutritional level, such as taioba and peixinho. Participants report community gardens as spaces that promote mental health, meeting and reflection, serving as an educational tool in the promotion of significant and permanent social changes, either through the form of production, through the collaborative management process and the fight against local food insecurity. Collective work is one of the ways to overcome food insecurity. It is necessary to take care of volunteers, increase engagement and improve their well-being. Between August and November/22 the focus will be to improve the ergonomic conditions of work in the gardens, to promote well-being and physical safety of those who work there. It is expected to reduce the turnover of participants and improve their comfort, thus contributing to the sustainability of these production systems.

    Como referenciar este item
    Icon

  • IPEN-DOC 29530

    TRINDADE, FABIANE J.; DAMASCENO, SERGIO; FLORIO, DANIEL Z.; FONSECA, FABIO C. ; FERLAUTO, ANDRE S.. Tuning of shape, defects, and disorder in lanthanum-doped ceria nanoparticles: implications for oxidative coupling of methane. In: CINE CONFERENCE, 3., 18-19 de outubro, 2022, Campinas, SP. Resumo... 2022.

    Abstract: The instability of petroleum prices and the advancements in extraction technologies of natural gas has increased the interest in the direct conversion of methane to C2 products via oxidative coupling of methane (OCM) reactions. The design of catalysts by tailoring the structural defects and disorder has a significant impact on their performance. Within this context, in this work, the fine-tuning of oxygen defects in rod-like NPs was performed via La3+ doping (LaxCe1-xO2-1/2), x, in the 10-70 mol% range. The NPs characterization was performed by SEM, HRTEM, XRD, BET and Raman spectroscopy, and the OCM catalytic performance was evaluated at 750 °C. The relative concentration of reagents (CH4 and O2) and products H2, CO, CO2, C2H4, and C2H6 was measured by an online mass spectrometer. XRD and Raman analysis revealed that the CeO2 fluorite crystalline structure is essentially maintained in the doped nanorods, even for x = 0.7. The Raman spectra analysis indicates that La doping results in the formation of extrinsic and intrinsic oxygen defects, which increase proportionally to La concentration. The catalysts showed good performance for OCM with methane conversion up to 32% and C2 selectivity up to 44% for x=0.5. In addition, all catalysts showed high stability within 20h time on stream. The demonstrated structural defect control on La-doped CeO2 NPs can provide important insights to improve the performance of OCM reactions.

    Palavras-Chave: doped materials; lanthanum; fracture mechanics; methane

    Como referenciar este item
    Icon

  • IPEN-DOC 29529

    TOMINAGA, F.K. ; BOIANI, N.F. ; SILVA, T.T. ; LEO, P.; BORRELY, S.I. . Application of electron beam irradiation for remediation of pharmaceutical compounds in water. In: CONGRESO ARGENTINO DE LA SOCIEDAD DE TOXICOLOGÍA Y QUÍMICA AMBIENTAL, 8th, March 7-11, 2022, Mar del Plata, Argentina. Abstract... Mar del Plata, Argentina: Universidad Nacional de Mar del Plata, 2022. p. 253-253.

    Abstract: A significant number of pharmaceutical active compounds have been released in the aquatic environment. These compounds are not fully removed from water and wastewater treatment plants. Furthermore, these contaminants are not commonly monitored, and they possess the potential to cause adverse ecological and human health effects. Electron Beam Irradiation (EBI) have been applied as an alternatively green method in water management, being efficient for removing organic recalcitrant pollutants at low doses. This work aims to assess the effect of EBI on toxicity of four pharmaceuticals from distinct class (anti-inflammatory, antidepressant, antibiotic, and antidiabetic) using organism from different trophic levels. Acetylsalicylic acid and fluoxetine hydrochloride were obtained from Labsynth (>99.5%) and Divis Pharmaceuticals Pvt. Ltd (98.8%), respectively. Metformin hydrochloride (97%) and ciprofloxacin (>98%) were purchased from Sigma- Aldrich. All aqueous solution were diluted using ultra-pure water. Acute toxicity assays with Daphnia similis and Vibrio fischeri were based on ABNT/NBR standard methods. The evaluated endpoint was immobility and bioluminescence inhibition, respectively. The yeast assays were carried by monitoring of changes in the specific conductivity of suspensions of S. cerevisiae. All the assays were performed in triplicate. The toxicity results of the microcrustacean and the bacteria were expressed in Toxicity Factor. For the yeast, data were analyzed by F-test and t-test using a significance level of 0.05. The UV-Vis spectrum showed changes in all pharmaceutical’s spectrum after irradiation at 2.5 kGy. The toxicity results indicated that the effects varied depending on the organism and the studied pharmaceutical. For acetylsalicylic acid, increase of toxicity was observed for all three the organism. In contrast, for fluoxetine, a great toxicity removal was achieved for D. similis while and for the bacteria and the yeast no changes of toxicity were noted. Regarding metformin, the radiolytic byproducts were only toxic to the microcrustacean, possibly including residual hydrogen peroxide. Finally, for ciprofloxacin, toxicity increase was verified for D. similis and V. fischeri, while detoxification was observed for S. cerevisiae. In conclusion, the present work demonstrated EBI is effective for removing pharmaceuticals and showed the importance of using different organism for toxicity assessment.

    Como referenciar este item
    Icon

  • IPEN-DOC 29528

    BOIANI, N.F. ; TOMINAGA, F.K. ; SILVA, T.T. ; REDÍGOLO, M.M. ; BORRELY, S.I. . Assessment of binary mixture toxicity of pharmaceuticals of environmental concern to aquatic organisms. In: CONGRESO ARGENTINO DE LA SOCIEDAD DE TOXICOLOGÍA Y QUÍMICA AMBIENTAL, 8th, March 7-11, 2022, Mar del Plata, Argentina. Abstract... Mar del Plata, Argentina: Universidad Nacional de Mar del Plata, 2022. p. 143-143.

    Abstract: Pharmaceutical products have been frequently detected in aquatic environments as mixtures. They may cause direct toxic effects to aquatic organisms and indirect effects on ecosystems, due to interactions which induce additive, synergistic or antagonistic effects. The classical mathematical models of concentration addition and independent action of pollutants have been extensively used for predicting the mixture effects of chemicals of environmental relevance. The objective of this study was to assess the binary mixture toxicity of pharmaceuticals: fluoxetine and propranolol; fluoxetine and sulfadiazine; fluoxetine and caffeine, in acute tests with Daphnia similis carried out based on ABNT/NBR standard methods. The prediction of the acute effects to binary mixture was performed considering the mentioned mathematical models and deviations of them (synergism/antagonism; concentration level-dependent or concentration ratio-dependent), using an automated Excel spreadsheet. For the mixture of propranolol + fluoxetine, the concentration addition model best described the mixture effects, which explained most of the variability of acute toxic responses. Through the concentration level-dependent, synergism at low doses and antagonism at high doses was pointed out. The concentration addition model better described the effects of the binary mixture of sulfadiazine + fluoxetine. Synergism related to the independent action model and an antagonism related to the concentration ratio-depend were identified. For caffeine + fluoxetine mixture, the concentration addition model described slightly better the effects of this binary mixture, synergism concentration level-dependent and dose ratio-dependent were identified. We can conclude that the nature of the interaction between the compounds of a mixture depends on effect level and on the ratio in which each one is applied. Major effects of the binary mixture were detected in the zone between the effects predicted by the evaluated models. The synergism/antagonism or additivity do not depend on the similarity/dissimilarity of the mode of action of the compounds of a mixture, showing a different behaviour respect to the theoretical assumptions.

    Como referenciar este item
    Icon

  • IPEN-DOC 29524

    NOGUEIRA, MARIA S.; SQUAIR, PETERSON L.; ENGLER, CAMILA; GOMES, DANIELE S.; LEYTON, FERNANDO; HWANG, SUY; SILVEIRA, RENATA; SILVEIRA, ROSANGELA C.; SOUZA, DIVANIZIA N.; POTIENS, MARIA P.A. . Evaluation of the mean glandular dose for digital breast tomosynthesis and Full-Field Digital Mammography in Brazil. In: EUROPEAN RADIATION PROTECTION WEEK, October 9-14, 2022, Estoril, Portugal. Abstract... 2022.

    Como referenciar este item
    Icon

  • IPEN-DOC 29483

    CALVO, WILSON A.P. ; SOMESSARI, SAMIR L. ; DUARTE, CELINA L. ; SPRENGER, FRANCISCO E. ; FEHER, ANSELMO ; LAINETTI, FABIANA de F. ; GASPER, RENATO R. ; BRAGA, ALCIDES; RODRIGUES, MARCOS; SAMPA, MARIA H.O. . Development and construction of a mobile electron beam accelerator to treat and recycle industrial effluents in Brazil. In: INTERNATIONAL CONFERENCE ON APPLICATIONS OF RADIATION SCIENCE AND TECHNOLOGY, 2nd, August 22-26, 2022, Vienna, Austria. Abstract... Vienna, Austria: International Atomic Energy Agency (IAEA), 2022.

    Abstract: In the world, there is a growing increase in the demand for water for human consumption, as well as the prioritization of the use of available water resources for public supply. The treatment of wastewater and industrial effluents by electron beam irradiation is a promising technique, however, not very widespread in Brazilian territory. The design and construction of a mobile unit by the Nuclear and Energy Research Institute (IPEN/CNEN), containing an electron beam accelerator of 0.7 MeV, 20 kW and 640 mm window is innovative to demonstrate the effects and positive results of this technology. The mobile unit will have as one of its main advantages the possibility of treating effluents in the place where the source is located, eliminating costs and bureaucratic problems associated with the transportation of waste, besides publicizing the technology in several places in the country. To implement the project, IPEN/CNEN has been consolidating partnerships with national and international companies. The resources for the development of the unit have been supplied by the Brazilian Innovation Agency (FINEP) and International Atomic Energy Agency, financing the “IAEA TC Project BRA1035 - Mobile electron beam accelerator to treat and recycle industrial effluents”. The Institute has associated with a specialized company (Truckvan Industry) in an innovation project for the unit design and development. Several meetings have been realized with the company and the International Atomic Energy Agency experts, aiming the compatibility of the design and the exchange of information necessary for the project development. The idealized project divides the cart in the following modules: a) control room and laboratory for technical and scientific dissemination of the technology; b) industrial electron beam accelerator, hydraulic units, ventilation system, cooler and bunker with irradiation device; and c) transformer and power source supply. A 3D model study of the control room and laboratory space was done to facilitate understanding the internal distribution of the laboratory analysis equipment (Gas Chromatography Mass Spectrometry, Total Organic Carbon and UV-Visible Spectroscopy). The irradiation system with electron accelerators allows treating different types of effluents. Depending on the effluent, the amount of ionizing radiation energy required for treatment may vary, as well as the amount of treated effluent per day. For the construction of the mobile unit, the estimated cost is about US$ 1.5 Million. The type of treated effluent, the treatment cost per m3/day and other information regarding the cost of maintenance and operation of the mobile unit are obtained from the Business Plan of the Mobile Unit.

    Palavras-Chave: electron beams; accelerators; portable equipment; water treatment; waste water; liquid wastes

    Como referenciar este item
    Icon

  • IPEN-DOC 29482

    CARDOZO, NELSON X. ; HARAGUCHI, MARCIO I.; KIM, HAE Y.; SOMESSARI, SAMIR L. ; FEHER, ANSELMO ; NAPOLITANO, CELIA M. ; CALVO, WILSON A.P. . Development of an irradiation system for production of gaseous radioisotopes and of a tomographic 2-D gamma scanning for industrial process troubleshooting in Brazil. In: INTERNATIONAL CONFERENCE ON APPLICATIONS OF RADIATION SCIENCE AND TECHNOLOGY, 2nd, August 22-26, 2022, Vienna, Austria. Abstract... Vienna, Austria: International Atomic Energy Agency (IAEA), 2022.

    Abstract: Radioisotopes as radiotracers are used in analytical procedures to obtain qualitative and quantitative data systems, in physical and physicochemical studies transfers, and troubleshooting of industrial process plants in chemical and petrochemical companies. In the production of gaseous radioisotopes used as tracers in industrial process measurements, argon-41 (41Ar) and krypton-79 (79Kr) stand out because each has low reactivity with other chemical elements. 41Ar is a transmitter range with high-energy (1.29 MeV) and a high percentage of this energy transformation (99.1%), resulting in relatively small quantities required in relation to the other, for an efficient detection, even in large thicknesses components. In this sense, the aim of this study is to develop an irradiation system for gaseous radioisotope production in continuous scale, applied in industrial applications of emission tomography and flow measurement. The irradiation system may produce 41Ar with activity of 7.4×1011 Bq (20 Ci) per irradiation cycle, through the Reactor IEA-R1 with 4.5 MW and average thermal neutron flux of 4.71×1013 ncm−2s−1 to meet an existing demand in NDT and inspections companies, and even needed by the Radiation Technology Centre, at IPEN/CNEN. The irradiation system consists of an aluminium irradiation capsule, transfer lines, needle valves, ringed connections, quick connectors, manometer, vacuum system, dewar, lead shielding, storage and transport cylinders, among other components. The irradiation system was approved in the leakage and stability tests (bubble test, pressurization, evacuation and with leak detector equipment. In the experimental production obtaining 1.07×1011 Bq (2.9 Ci) of 41Ar, alanine dosimeters were distributed into various components of the irradiation system. In addition, exposure rates were determined in the lead shielding wall, in which the liquefied radioactive gas was concentrated, and in the storage and transport cylinders after 41Ar was transferred by the portable radiation meter. However, gamma scanning is a nuclear inspection technique widely used to troubleshoot industrial equipment in refineries and petrochemicals plants such as distillation columns and reactors. A sealed radiation source and detector move along the equipment, and the intensity readouts generate the density profile of the equipment. The result of gamma scan still consists of a simple 1-D density plot. In this work, we also present the tomographic gamma scanning that, using image reconstruction techniques, shows the result as a 2-D image of density distribution. Clearly, an image reveals more features of the equipment than a 1-D graph and many problems that could not be troubleshooted using the conventional technique can now be solved with this imaging technique. We use ART (Algebraic Reconstruction Technique) intercalated with total variation minimization filter. The use of total variation minimization leads to compressive sensing tomography, allowing to obtain good quality reconstruction from few irradiation data. We simulated the reconstruction of different density distributions. We applied the new technique to data obtained by irradiating with gamma rays phantoms that emulate industrial equipment. Finally, we present the result obtained by applying the innovative technique to real operating distillation column.

    Palavras-Chave: radioisotopes; isotope production; argon 41; radioisotope scanners; tomography; distillation

    Como referenciar este item
    Icon

  • IPEN-DOC 29481

    KODAMA, YASKO ; BIANCOLLI, ANA L. ; BARBOSA, ANDREY ; SANTIAGO, ELISABETE . Effects of gamma radiation absorbed dose on VBC grafting onto LDPE films and on anion exchange membrane fuel cells performance. In: INTERNATIONAL CONFERENCE ON APPLICATIONS OF RADIATION SCIENCE AND TECHNOLOGY, 2nd, August 22-26, 2022, Vienna, Austria. Abstract... Vienna, Austria: International Atomic Energy Agency (IAEA), 2022.

    Abstract: Anion-Exchange Membranes (AEMs) are polymer electrolytes that conduct anions, such as OH- and Cl-, as they contain positively charged [cationic] groups (typically) bound covalently to a polymer backbone. It is possible to take advantage of this characteristic for electrochemical devices, such as fuel cells. Fuel cells are electrochemical systems that convert chemical energy into electrical energy through continuous feeding fuel. These systems represent an alternative technology of electricity with great efficiency and large applicability in the areas of portable, stationary, and automotive powers due to important improvements achieved in the field of electrocatalysis in the past decade. In this present research, AEMs based on films of low density polyethylene (LDPE), were synthesized by radiation induced grafting with 4-vinylbenzylchloride (VBC) monomer solution using direct method. Samples were irradiated with gamma rays from Co-60 at room temperature, in nitrogen and/or air atmosphere at Gammacell of IPEN-CNEN/SP. Several radiation absorbed doses (10-30kGy) were used to study degree of grafting (DoG) of VBC onto LDPE gamma irradiated films. The optimization of parameters and conditions of irradiation have been evaluated by degree of grafting. Functionalization with quaternary ammonium groups was made in water by using trimethylamine (TMA). After, treatment with NaCl for Cl-groups addition was performed, responsible for ionic exchange and ionic groups stabilization. Then, hydroxylation reaction was performed with KOH for hydroxyl groups addition (anionic exchange of Cl- groups by OH-). Besides DoG, characterization of the ion exchange membranes was performed at IPEN-CNEN/SP involving determination of physico-chemical properties such as water uptake, ion exchange capacity and ionic conductivity. Furthermore, AC impedance spectroscopy, scanning electron microscopy (SEM) were performed for characterization of radiation grafted membranes. DoG increased with radiation absorbed dose increase, homopolymerization affected some grafted membranes at high radiation absorbed doses. Also, atmosphere of irradiation affected the DoG. The resulting AEM synthesized by RIG of VBC onto LDPE with 30 kGy radiation absorbed was tested in a fuel cell and reached a maximum power density of 942 mW cm−2 at 80 ℃ with gases flow of 0.8 L min-1 and 0.5 L min-1 for H2 and O2, respectively.

    Palavras-Chave: anions; ion exchange; membranes; fuel cells; gamma radiation; electrochemical cells

    Como referenciar este item
    Icon

  • IPEN-DOC 29480

    SAKATA, SOLANGE ; JACOVONE, RAYNARA M.S. ; DUARTE, MIGUEL ; GARCIA, RAFAEL ; ANGNES, LUCIO. Reduced graphene oxide obtained by gamma radiation to produce screen printed glucose biosensor. In: INTERNATIONAL CONFERENCE ON APPLICATIONS OF RADIATION SCIENCE AND TECHNOLOGY, 2nd, August 22-26, 2022, Vienna, Austria. Abstract... Vienna, Austria: International Atomic Energy Agency (IAEA), 2022.

    Abstract: Screen printed biosensor has attracted attention as point care device due to its fast and accurate response in a compact portable platform. Due the high electrical carrier mobility, reduced graphene oxide (rGO) has been used to modify the working electrode surface and increase the biosensor sensibility. However, there are some disadvantages during the reduction of graphene oxide that include the use of chemical reductants that need to be removed after the reaction and the toxic residues. The purification usually requires tedious steps and a lot of efforts to recover the nanomaterial. In this work screen printed carbon electrode (SPCE) was modified using rGO produced by gamma radiation. Graphene oxide (GO) was prepared by modified Hummers and the reduction was performed in a water/isopropanol solution and inert medium. Sample was irradiated in the Multipurpose Gamma Irradiation Facility at Radiation Technology Center from Nuclear and Energy Research Institute (IPEN/CNEN-SP), a category IV gamma irradiator by the IAEA classification under the radiation dose at 80,0 kGy. rGO characterization was performed by X-Ray Diffraction (XRD). From the XRD patterns: the 2θ the peak located at 11o shift to 23o, demonstrating the reduction of GO to rGO. The decrease of the distance between layers was attributed on partial remove of the oxygen groups from GO. For the glucose biosensor preparation, first of all, the SPCE (Metrohm, model 6. 1208. 110) was modified by drop-coating rGO solution and dried at room temperature for 24h. Then, for Glucose Oxidase (GOx) immobilization, the carboxylic groups from rGO were activated using N-hydroxysuccinimide (NHS) and 1-Ethyl-3-(3-dimethyalaminopropyl) carbodiimide (EDC) for 30 min at room temperature, followed by adding GOx 10KU from A. niger Type II (5mg/mL). The solution was incubated at 4oC overnight. SEM images showed GOx onto SPCE surface and the electrocatalysis of GOx toward glucose was measured to confirm the enzymatic activity. For electrochemical studies, cyclic voltammetry was carried out in a Portable Potentiostat model 910 PSTAT mini, Metrohm and PSTAT software. The fabricated amperiometric biosensor detects glucose ranged from 1mM to 5mM with LOD of 0.9 mM at 0.70V. Moreover, the biosensor exhibited repeatability, reproducibility and practicability. This study showed that rGO synthesized by gamma radiation without any further purification is a simple and sustainable approach to fabricate electrode for biosensors.

    Palavras-Chave: gamma radiation; graphene; glucose; biological indicators; sensors

    Como referenciar este item
    Icon

  • IPEN-DOC 29479

    UEHARA, VANESSA B. ; DEL MASTRO, NELIDA L. . Sustainable packaging materials for the food industry: role of radiation technology. In: INTERNATIONAL CONFERENCE ON APPLICATIONS OF RADIATION SCIENCE AND TECHNOLOGY, 2nd, August 22-26, 2022, Vienna, Austria. Abstract... Vienna, Austria: International Atomic Energy Agency (IAEA), 2022.

    Abstract: Food packaging keeps food safe and fresh and protects products during transport, delivery, and storage. On the other hand, packaging also fills trash containers and landfills, lasting far longer than the products it was made to contain and can also transfer chemicals into our food, with unknown health effects. Disposing massive quantities of wastes generated by nonbiodegradable packaging material pave ways for the study of biopolymers as alternative materials for food packaging. Ionizing radiation is the most effective means to disinfect dry food ingredients and an adequate phytosanitary treatment of food and agricultural commodities. The irradiation of food products is today a mature technology, effective, broad spectrum, residue free that can play an important role in food safety and food security. On the other hand, radiation processing of polymers is an attractive option for the food packaging industry worldwide. Practically every type of physical and chemical assay technique has been utilized for radiation studies on biological molecules. The aim of this article is to present some aspects of our own work on the development of edible films based on a combination of polysaccharide/protein - cassava starch and soy isolate protein - among the general application of irradiation technology on packaging materials.

    Palavras-Chave: food industry; packaging; biodegradation; food processing

    Como referenciar este item
    Icon

  • IPEN-DOC 29478

    GARCIA, RAFAEL H.L. ; CARNEIRO, FELIPE W. ; JACOVONE, RAYNARA M.S. ; SAKATA, SOLANGE K. ; ANGNES, LUCIO. The influence of gamma radiation doses on rGO/Ni for energy storage applications. In: INTERNATIONAL CONFERENCE ON APPLICATIONS OF RADIATION SCIENCE AND TECHNOLOGY, 2nd, August 22-26, 2022, Vienna, Austria. Abstract... Vienna, Austria: International Atomic Energy Agency (IAEA), 2022.

    Abstract: Metallic nanoparticles (MN) have been the subject of intense research in the last decades because of their high catalytic activity, associated with its large surface-to-volume ratio. However, it is difficult to obtain pure active metal nanoparticles surfaces, since its contamination and aggregation frequently result in deactivation and loss of catalytic activities. MN immobilization can minimize these effects. Thermal, mechanical, and electrical properties of reduced graphene oxide (rGO) make this material an excellent candidate for MN support for batteries and supercapacitors. The production of metallic nanoparticles on rGO surface based on gamma radiation allows less formation of residual impurities, absence of reducing agents, uniform mass production, no aggregation and low costs. Herein, we demonstrated a green and efficient one-step, gamma radiation-based method for Ni/rGO production. Graphene oxide (GO) was prepared by modified Hummers method. Ni+2 and graphene oxide reduction were performed in water/isopropanol solution (1:1) under inert medium. Samples were irradiated in the Multipurpose Gamma Irradiation Facility at CETER/IPEN/CNEN-SP, a category IV gamma irradiator by the IAEA classification, in radiations doses of 20, 40, and 80 kGy, at 10 kGy/h. From X-ray diffraction, the correlation between radiation doses and crystallite size was evaluated. For supercapacitor studies, cyclic voltammetry was carried out in a screen printed carbon electrode (SPCE) in K₄[Fe(CN)₆]. The working electrode was modified with produced rGO-Ni 80 kGy, Pt wire was used as counter and Ag/AgCl as reference electrode. Experiments were performed in KOH 1.0 M. Results showed that produced Ni/rGO has good potential to be used in electrochemical devices such as supercapacitors or batteries.

    Palavras-Chave: nanoparticles; graphene; gamma radiation; radiation doses

    Como referenciar este item
    Icon

  • IPEN-DOC 29477

    VASQUEZ, PABLO A.S. ; VIEIRA, ANA C.; LIMA, LENI; NAGAI, MARIA L.; KODAMA, YASKO ; OLIVEIRA, MARIA J. ; SANTOS, PAULO . New trends and applications of ionizing radiation for preservation of cultural heritage tangible materials. In: INTERNATIONAL CONFERENCE ON APPLICATIONS OF RADIATION SCIENCE AND TECHNOLOGY, 2nd, August 22-26, 2022, Vienna, Austria. Abstract... Vienna, Austria: International Atomic Energy Agency (IAEA), 2022.

    Abstract: Disinfection of cultural heritage artefacts and archived materials using ionizing radiation has been successfully applied and accepted by the Brazilian conservation and preservation institutions and community in recent years. Several works of art, museum collections artefacts, books, manuscripts, drawings, archive documents, musical instruments, ethnographic objects, archaeological findings and natural history collections have been decontaminated. Several research studies have been developed addressing the behaviour of cellulosic based materials treated with the ionizing radiation. However, many Brazilian collections have objects made from the most diverse constitutive materials and these are affected by biodeterioration. In order to the effective decontamination of the most diverse types of objects there was a need to establish protocols for care of institutions and individuals carrying cultural and historical collections and for the effective processing by ionizing radiation in the facilities respecting the ethical principles of conservation and restoration covering activities from the problem detection to the final cleaning. Additionally, ionizing radiation has allowed the development of innovative nanostructured cross-linked materials, with applicability in cleaning delicate surfaces. This work presents the most recent results of the effect of ionizing radiation on morphology and physicochemical properties of photographic and cinematographic films, featherwork and botanical collections – exsiccate; as well as the protocols developed as a practical guide for conservatives and professionals of the irradiation for treatment of tangible objects. Preliminary results of nanostructured cross-linked polymeric hydrogels for cleaning surface of artworks obtained by ionizing radiation are also presented. The IAEA sponsored projects have helped to increase the acceptance of nuclear technology by the Brazilian society, especially by the community of restorers, curators and conservators of material cultural goods. The research developed and disseminated with the support of IAEA contributes to the understanding that the cultural heritage is the legacy of physical artefacts and intangible attributes of a group or society that are inherited from past generations, maintained in the present and restored for the benefit of future generations.

    Palavras-Chave: cultural objects; preservation; sterilization; ionizing radiations

    Como referenciar este item
    Icon

  • IPEN-DOC 29476

    NAGAI, MARIA L.; SALVADOR, PABLO A.V. . Preservation and conservation of information contained in glassy materials from cultural collections with processing by ionizing radiation. In: IAEA WORKSHOP ON INNOVATIVE APPROACHES OF ACCELERATOR SCIENCE AND TECHNOLOGY FOR SUSTAINABLE HERITAGE MANAGEMENT, June 13-16, 2022, Vienna, Austria. Abstract... Vienna, Austria: International Atomic Energy Agency (IAEA), 2022. p. 29-29.

    Abstract: Glass-based photographic materials are commonly found in historical cultural heritage collections.Inadequate storage conditions for these materials lead to problems of biodeterioration, mainly by fungi. Processing by ionizing radiation with electron beam has a biocidal effect to combat fungal contamination. However, a known undesired effect on glassy materials is increased opacity which affects the readability of images on photographic glass negatives. In this way, the study proposes a methodology to minimize the darkening effects of the glasses that are subjected to irradiation. For this work, glass samples were subjected to irradiation with electron beams at doses of up to 25 kGy, under different controlled temperature conditions, and the effects analyzed by UV-visible spectrophotometry and colorimetry analysis.

    Palavras-Chave: cultural objects; preservation; sterilization; cultural resources; ionizing radiations

    Como referenciar este item
    Icon

  • IPEN-DOC 29475

    VIEIRA, ANA C.D. ; SALVADOR, PABLO A.V. ; SANTOS, PAULO de S. . Moving toward a sustainable conservation: experience of the Museum of Archeology and Ethnology (MAE-USP). In: IAEA WORKSHOP ON INNOVATIVE APPROACHES OF ACCELERATOR SCIENCE AND TECHNOLOGY FOR SUSTAINABLE HERITAGE MANAGEMENT, June 13-16, 2022, Vienna, Austria. Abstract... Vienna, Austria: International Atomic Energy Agency (IAEA), 2022. p. 30-31.

    Abstract: Most of the ethnographic collections present in traditional museums in Brazil were formed by collectors, purchases, donations and exchanges by large encyclopedic, naturalist museums, typical of the 19th century. It is in this context, the Museum of Archeology and Ethnology (MAE-USP) collection were constituted. The MAE has been made a big effort to guarantee their conservation. Preservation of tangible objects as well thousands of organic objects, composed of plant fibers, wood, skins, feathers, seeds and various materials, has always been challenging as they are prone to deterioration by biological agents such as insects, mold, bacteria and rodents. Chemical treatments are traditionally examples of actions to preserve many museum collections around the world. Since the 19th century, collectors and museum professionals have applied a variety of toxic substances through fumigations and direct applications trying to enhance the conservation. Although a well-intentioned practice, the application of pesticides to protect ethnographic objects could not predict the negative impact on the safety of those who would handle these objects in the future and by restricting the possibilities of using these collections by the descendants of their creators. Today, these contaminated objects cannot be touched without gloves or experienced by for example, indigenous groups. The current insertion of native communities in curatorial actions at museums has made it possible to renew the way in which these institutions work. At this moment, it is no longer plausible that a museum institution continues to carry out toxic treatments on funerary, sacred objects, human remains, among others. The possession and use of these objects transcend the museum’s borders and the possibilities of use must be considered in the perspective of the future. Due to the renewal of the theoretical parameters of the conservation discipline, the Integrated Pest Management policy is more suitable for museological institutions. Efforts to prevent damage have been more effective than just thinking about curative conservation. In addition, the need to develop a more sustainable present and future has led institutions to develop greener prevention policies, without the use of toxic products, respecting the environment and the user. In this scenario, since 2010, MAE-USP has abolished the use of pesticides to treat the collection. Since then, the institution has been dedicated to building a protocol to reduce risks related to infestations. This protocol encompasses, among other actions, the treatment of objects affected by biological agents and the preventive treatment of new objects through ionizing radiation. The use of ionizing radiation for the disinfestation of museum objects is a very safe process and has proved to be a great alternative to traditional methods of disinfestation that involve pesticides of high persistence and toxicity. For this reason, we have also worked to disseminate the technique among conservators. This work intends to share the actions carried out by MAE-USP in partnership with the Nuclear and Energy Research Institute (IPEN) to facilitate the treatment of ethnographic objects, as well as an important collaborator to make the conservation process at MAE more sustainable.

    Palavras-Chave: cultural objects; archaeological specimens; historical aspects; sterilization; pesticides

    Como referenciar este item
    Icon

  • IPEN-DOC 29474

    VASQUEZ, PABLO A. ; NAGAI, MARIA L.. Ionizing radiation for preservation: uses of electron-beam technology for conservation of photographic and cinematographic films. In: IAEA WORKSHOP ON INNOVATIVE APPROACHES OF ACCELERATOR SCIENCE AND TECHNOLOGY FOR SUSTAINABLE HERITAGE MANAGEMENT, June 13-16, 2022, Vienna, Austria. Abstract... Vienna, Austria: International Atomic Energy Agency (IAEA), 2022. p. 3-3.

    Abstract: The Nuclear and Energy Research Institute – IPEN through the Multipurpose Gamma Irradiation Facility and the Electron Beam Irradiation Facilities has disinfected several tangible cultural collections from the University of São Paulo – USP. Brazilian weather conditions added to the actions of insects and fungi promote biodegradation especially in cellulose based materials. In this sense, ionizing radiation is an excellent alternative to the traditional preservation process mainly because the biocidal action. Electron beam irradiation also presents new possibilities for processing materials with greater speed, despite having limited penetration. Adequate storage of photographic and cinematographic materials is a challenge for experts from preservation institutions. Contamination by fungi is one of leading causes of problem in this kind of collections. In addition, another common physicochemical degradation affecting cellulose triacetate films causing deacetylation of polymer chain is called “vinegar syndrome”. In this work are presented results of the effect of the electron beam irradiation on photographic and cinematographic films using an electron beam accelerator with energy of 1.5 MeV and beam power of 37.5 kW. Selected film samples were characterized by FTIR-ATR spectroscopy and FEGSEM-EDS microscopy. Samples were irradiated with absorbed dose between 2 kGy and 200 kGy. Irradiated samples were analyzed by UV-Vis spectrophotometry, FEGSEM, thermogravimetric analysis (TG) and differential scanning calorimetry (DSC). Results showed that disinfection by electron beam radiation can be achieved safely applying radiation absorbed doses between 6 kGy to 10 kGy with no significant change or modification of main properties of the constitutive polymeric materials. Electron beam irradiation, due to the effect of crosslinking is presented as an alternative to treat films affected by “vinegar syndrome” applying absorbed dose of 80 kGy in order to increase shelf life of cultural heritage materials.

    Palavras-Chave: cultural objects; historical aspects; ionizing radiations; electron beams

    Como referenciar este item
    Icon

  • IPEN-DOC 29460

    GALDINO, G.S. ; RODRIGUES, W.C. ; CRUZ, P.D. ; CASINI, J.S.; SAKATA, S.K. ; FARIA, R.N. . Characteristics of electric double layer capacitors prepared with electrolytes based on deep eutectic solvents. In: CONGRESSO BRASILEIRO DE ENGENHARIA E CIÊNCIA DOS MATERIAIS, 24., 6-10 de novembro, 2022, Águas de Lindóia, SP. Resumo... 2022.

    Abstract: The storage capacity of electric double layer capacitors or electrochemical supercapacitors with electrolytes based on deep eutectic solvents (DES) composed of L-lactic acid with several hydrogen bond acceptors (nicotinamide, L-alanine, ammonium acetate, sodium acetate, choline chloride, amino acetic acid) with a molar ratio of 7:1 have been investigated. A DES based on urea and choline chloride with at a molar ration of 2:1 has also been included for comparison. The electrochemical supercapacitors were prepared using commercial activated carbon electrodes after removing the volatile organic electrolyte with a vacuum pump. The characteristics of the electrochemical supercapacitors were determined by cyclic voltammetry at temperature room temperature and also after heating at 353 K using scan rates that varied from 2 to 25 mVs-1. Lowest scan rate led to higher specific capacitance of 150±8 Fg-1 with a maximum applied potential of 1.7 V for the urea and choline chloride DES with a molar ration of 2:1. The lactic acid with all the hydrogen bond acceptors with a molar ratio 7:1 it has been necessary to increase the temperature above room temperature to improve the specific capacitance.

    Palavras-Chave: electrolytes; capacitors; layers; electrochemical cells

    Como referenciar este item
    Icon

  • IPEN-DOC 29459

    PRADO, F.M. ; UTIYAMA, K.A.; TOFFOLI, D.J.; DA SILVA, S.L.. Dispositivo automático para melhoria do processo de coleta de dados com amostras fotoelásticas de pequenas dimensões em técnicas de fotoelasticidade. In: CONGRESSO BRASILEIRO DE ENGENHARIA E CIÊNCIA DOS MATERIAIS, 24., 6-10 de novembro, 2022, Águas de Lindóia, SP. Resumo... 2022.

    Abstract: Os materiais fotoelásticos possuem a propriedade da birrefringência e, devido principalmente à sua transparência e elasticidade, são largamente utilizados na indústria metalomecânica para determinação indireta de propriedades de materiais, por técnicas e métodos de Fotoelasticidade, uma sub área da Óptica que proporciona a investigação da relação entre os fenômenos de birrefringência temporária e elasticidade mecânica. No entanto, há dificuldades associadas às medições das variáveis envolvidas durante a interação da luz com a amostra quando uma tradicional máquina universal de ensaios é utilizada para efetuar os esforços, sem mencionar o fato de que amostras de pequenas dimensões não são bem aceitas em tais ensaios mecânicos. Neste contexto, foi construído um dispositivo automático que permite a compressão das amostras de pequenas dimensões por uma amplitude pré-determinada de cargas associado à captura de vídeos com informações fotoelásticas. Com isto, foi possível obter valores de propriedades dos materiais fotoelásticos, como módulo de elasticidade e coeficiente de Poisson, com acurácia em torno de 20% melhor, comparado a trabalhos da literatura, devido a maior resolução proporcionada pelo dispositivo e, também, pela elevada quantidade de dados obtidas, quando comparada a processos manuais.

    Palavras-Chave: photoelasticity; poisson ratio; automation

    Como referenciar este item
    Icon

  • IPEN-DOC 29458

    PRADO, F.M. ; UTIYAMA, K.A.; TOFFOLI, D.J.; OUTA, R.; DA SILVA, S.L.. Método computacional para determinação do coeficiente relativo de dispersão fotoelástica em amostras de materiais fotoelásticos a partir dos dados de fotoelasticidade por transmissão. In: CONGRESSO BRASILEIRO DE ENGENHARIA E CIÊNCIA DOS MATERIAIS, 24., 6-10 de novembro, 2022, Águas de Lindóia, SP. Resumo... 2022.

    Abstract: Segundo a Lei óptica das tensões, o coeficiente relativo de dispersão fotoelástico é o resultado da relação entre as diferenças de tensões internas e as diferenças de índices de refração, provocadas por esforços externos, em materiais fotoelásticos, que têm a propriedade da birrefringência temporária. Em geral o coeficiente relativo é uma propriedade do material e função do comprimento de onda da luz que o atravessa. Em Fotoelasticidade, há uma grande dificuldade em encontrar este coeficiente, pois as determinações dos parâmetros ópticos são muito complicadas e indiretas, levando a resultados com baixa acurácia. Este trabalho mostra que existe uma relação direta entre os esforços externos e as tensões internas e, também, entre as deformações internas e os índices de refração. Consequência disto, o coeficiente relativo de dispersão fotoelástica pôde ser determinado pela relação entre os esforços externos e as deformações estatísticas internas. Os resultados foram comparáveis à literatura com uma melhoria da acurácia da ordem de 15%, devido à utilização de um método computacional fundamentado em teoria de erros e um processamento que utilizou uma grande quantidade de informações cedidas por uma técnica de fotoelasticidade por transmissão que utilizou um dispositivo automático para compressão das amostras.

    Palavras-Chave: photoelasticity; computer calculations; accuracy; deformation

    Como referenciar este item
    Icon

  • IPEN-DOC 29457

    BARROS, G.D. ; NETO, R.M. ; BARDELLA, F. . Use of CrystalWalk interactive program to teach crystal structures to Brazilian students in engineering courses. In: CONGRESSO BRASILEIRO DE ENGENHARIA E CIÊNCIA DOS MATERIAIS, 24., 6-10 de novembro, 2022, Águas de Lindóia, SP. Resumo... 2022.

    Abstract: Understanding how atoms of chemical elements form crystals is not a simple task, especially for students that are learning about the properties of materials. This task is even more arduous when the course workload does not match the content and traditional teaching methods do not stimulate students. Recently, the teaching methodology of Materials Science discipline has been completely modified aiming at the improvement of student learning. The new procedure is based on project execution, team-based learning, flipped classroom, use of the CrystalWalk interactive program and additive manufacturing, with 3D printing of crystal structure models. CrystalWalk is free didactic-interactive software for synthesis and visualization of crystalline structures, developed by the Nuclear and Energy Research Institute (Ipen, Brazil), which can be used online and in devices such as computers, tablets, and smartphones. With the software it is possible to construct the crystals step by step, choosing the basic structures and the atoms that will enter its composition. In the first step, questions were presented using the Plickers application to check student’s prior knowledge before approaching the topic and then a video-lesson with key points about crystalline structures was visualized in the flipped classroom model. After that, groups were created to construct the different structures using the CrystalWalk software, and the 3D impression of the structures of each group was built. Finally, new questions about crystalline structure were presented using the Peer instruction methodology to evaluate if 70% of the class absorbed the presented content. As a result, it was observed that the use of this new learning model increased the student’s engagement and, consequently, their final grades.

    Palavras-Chave: crystal structure; crystallography; computer codes

    Como referenciar este item
    Icon

  • IPEN-DOC 29456

    CRUZ, C.P. ; FREITAS, L.F. de ; FERREIRA, A.H. ; ALVES, V.M. ; LUGAO, A.B. . Estudo das sínteses por feixe de elétrons e radiação gama para obtenção de nanopartículas de albumina. In: CONGRESSO BRASILEIRO DE ENGENHARIA E CIÊNCIA DOS MATERIAIS, 24., 6-10 de novembro, 2022, Águas de Lindóia, SP. Resumo... 2022.

    Abstract: Atualmente, as proteínas têm sido alvo de estudos na área da nanotecnologia uma vez que possuem propriedades de grande interesse para área biomédica como alta biocompatibilidade e baixa toxicidade, dentre outras. Estratégias envolvendo a produção de nanopartículas direcionadas ao desenvolvimento de sistemas de entrega inteligentes visando aumentar a biodisponibilidade, especificidade ao tecido alvo e maior tempo de circulação sistêmica é alvo constante de pesquisas em nanomedicina. Nesse sentido, nanopartículas a base de proteínas plasmáticas mostram-se mais vantajosas frente a nanopartículas de materiais sintéticos, visto que possuem propriedades mais adequadas para aplicações in vivo como baixa toxicidade, biodegradabilidade e geralmente não desencadeiam respostas imunes. Comercialmente estão disponíveis alguns produtos: Nanocoll® (reagente liofilizado para detecção de linfonodo sentinela quando radiomarcado) e Abraxane® (paclitaxel ligado à albumina). O uso de radiação como rota alternativa para promover a reticulação de partículas proteicas tem sido amplamente discutido e tem demonstrado a possibilidade de preservação das estruturas proteicas e manutenção de suas atividades biológicas. Síntese induzida via radiação demonstram-se mais vantajosas, pela ausência de uso de reticulantes químicos, diminuindo assim a toxicidade residual e mostrando-se eficaz no controle de tamanho. Os efeitos da irradiação de proteínas, especialmente em solução aquosa, envolvem a geração de espécies reativas proveniente da radiólise da água ou solvente, que são capazes de promover a reticulação. Os tamanhos das nanopartículas podem oferecer melhores propriedades químicas, físicas e biológicas, por esse motivo o estudo teve como objetivo um melhor entendimento no uso de diferentes tipos de irradiações e condições de síntese para avaliar os efeitos relacionados ao controle de tamanho das nanopartículas. Para avaliação das nanopartículas as sínteses foram realizadas variando a concentração protéica de 0,5 a 10mg/mL, em diferentes tampões fosfato e o tris-HCl ambos em concentração de 50mM. Após a preparação das amostras, as mesmas foram submetidas a processos de irradiação distintos: feixes de elétrons (E-Beam) e radiação gama (irradiador multipropósito 60-Co), com variação de dose de 10 a 20kGy. As amostras foram analisadas pela técnica de espalhamento dinâmico de luz (DLS), com o intuito de avaliar o seu tamanho hidrodinâmico e índice de polidispersão das diferentes condições sintéticas. Os resultados obtidos para o tampão fosfato demonstraram que as condições sintéticas propostas não afetam consideravelmente o tamanho das nanopartículas. Para as nanopartículas com Tris-HCl o tamanho é diretamente afetado pela concentração de proteína, dose de irradiação e tipo de radiação utilizada. As nanopartículas obtidas por radiação gama demonstram-se obter tamanhos mais monodispersos em relação às obtidas por feixe de elétrons.

    Palavras-Chave: electron beams; albumins; nanoparticles; gamma radiation; synthesis

    Como referenciar este item
    Icon

  • IPEN-DOC 29455

    BARROS, G.D. ; CREMASCO, I.K.; FERREIRA, M.P.; JUNIOR, R.A.. Utilização de nanofibras de EPS reciclado produzidas por rotofiação na filtragem de óleo em água. In: CONGRESSO BRASILEIRO DE ENGENHARIA E CIÊNCIA DOS MATERIAIS, 24., 6-10 de novembro, 2022, Águas de Lindóia, SP. Resumo... 2022.

    Abstract: A presente pesquisa cientifica tem como objetivo o estudo da utilização de nanofibras de EPS reciclado como alternativa de filtragem de óleo em água. Foram analisadas nanofibras de EPS reciclado produzidas por rotofiação com diferentes concentrações de EPS e verificadas por meio de microscopia eletrônica de varredura. Desta forma, é proposta uma solução alternativa para a filtragem de óleo em água, bem como para a reciclagem do EPS, que é um material com longo período para a decomposição, e é pouco reciclado devido o alto custo no processo decorrente da sua baixa densidade. O uso do EPS reciclado em forma de fibras, além de contribuir para. Ao fim da pesquisa, foi possível identificar as variações das concentrações de EPS capazes formar fibras para serem utilizadas como filtro.

    Palavras-Chave: nanofibers; polystyrene; filters

    Como referenciar este item
    Icon

  • IPEN-DOC 29454

    SILVA, J.T. ; NOGUEIRA, B.R. ; ANGELOCCI, L.V. ; SOUZA, C.D. ; TEODORO, L.E. ; SOUZA, P.D. ; RODRIGUES, B.T. ; CORREIA, R.W. ; SANTOS, H.N. dos ; ZEITUNI, C.A. ; ROSTELATO, M.E. . Avaliação do método produtivo de placas de epóxi com fósforo-32 para o tratamento do câncer espinhal e intracranial por braquiterapia. In: CONGRESSO BRASILEIRO DE ENGENHARIA E CIÊNCIA DOS MATERIAIS, 24., 6-10 de novembro, 2022, Águas de Lindóia, SP. Resumo... 2022.

    Abstract: A braquiterapia é uma modalidade de radioterapia utilizada no tratamento do câncer. Nessa modalidade, a fonte radioativa é posiciona junto ao tumor ou bem próxima a ele. A dose de radiação é entregue de forma contínua em um período curto de tempo (fontes temporárias) ou em períodos mais longos durante todo o decaimento radioativo do material (fontes permanentes). A maior vantagem da braquiterapia, é o fato da fonte estar bem próxima ao tumor o que significa que a região alvo recebe a maior parte da dose protegendo os tecidos sadios adjacentes à região tumoral. Shtrombakh et. al. trabalharam com césio-137 e verificaram que o uso do epóxi para a imobilização de fontes radiativas ocorreu sem vazamento por dois anos de testes. Pesquisas realizadas nos Estados Unidos por Folkert et. al. mostraram que placas flexíveis incorporadas com fósforo-32 são alternativas para o tratamento de câncer do sistema nervoso central na fase intraoperatória. No presente trabalho foi avaliada a uniformidade da placa de resina epóxi a partir de uma metodologia desenvolvida no Laboratório de fontes para Braquiterapia do IPEN/CNEN- SP. Vários testes foram realizados para determinar o melhor molde para a fabricação da placa. Concluiu-se que o politetrafluoretileno (PTFE), que comercialmente é conhecido como teflon foi o que obteve melhor resultado, devido a facilidade para desenformar a fonte após o processo de cura da resina. As placas de epóxi foram produzidas a resina 2220 e catalisador 3154 (Avipol), à proporção de 2:1 (massa). Para simular o material radioativo, ácido clorídrico (HCl) equivalente a 5 % da massa total (resina + catalisador) é acrescentado. O processo de cura da resina epóxi foi durante 24 h sob temperatura ambiente. As espessuras das placas foram medidas chegando-se a um valor médio de 0,300 mm ± 0,070. As medidas foram efetuadas com micrômetro medindo-se 10 pontos de cada placa. As medidas de largura e comprimento não foram realizadas, pois esses parâmetros não influenciam na uniformidade da dose. Para que a distribuição da atividade do fósforo-32 fosse estipulada, uma simulação por Método de Monte Carlo utilizando o código MCNP foi realizada. A variação máxima de dose ao longo da placa, considerando uma espessura totalmente uniforme de 0,300 mm, resultou em < 0,5 % até 0,5 cm antes da borda. O resultado da simulação mostra que com uma placa de espessura uniforme, a tendência da distribuição de dose seja homogênea. Pautando-se nos resultados, as placas de polímero epóxi se mostram viáveis para o uso em braquiterapia, sendo que o próximo passo do trabalho será os testes com material radioativo, a avaliação por métodos dosimétricos físicos e computacionais.

    Palavras-Chave: epoxides; brachytherapy; teflon; polytetrafluoroethylene; catalysis

    Como referenciar este item
    Icon

  • IPEN-DOC 29453

    BARROS, G.D. ; PEREIRA, V.M.; OLIVEIRA, J.d.; ARAUJO, C.T.; PEREIRA, W.d.; MACHADO, L.S.. Uso de inteligência artificial na análise da morfologia de nanofibras. In: CONGRESSO BRASILEIRO DE ENGENHARIA E CIÊNCIA DOS MATERIAIS, 24., 6-10 de novembro, 2022, Águas de Lindóia, SP. Resumo... 2022.

    Abstract: Os estudos e aplicações de nanofibras tem crescido ao longo dos anos, neles, foi observado que as propriedades desses fios em escala nanométrica, possuem vantagens em aplicações em diversas áreas como a biomédica, armazenamento e produção de energia e aplicações que envolvem a filtragem de água e ar. Esses materiais são sintetizados através de processo, também chamados de técnicas e por conta disso estão sujeitos a apresentação de falhas. Sendo as falhas mais comuns, a formação de beads e poros. A partir disso, é necessário que seja realizada a caracterização morfológica da nanofibras após sua produção, a fim de identificar essas falhas. Com a evolução da computação, aplicações utilizando inteligência artificial baseada em técnicas de aprendizagem de máquina tem crescido exponencialmente. Essas técnicas permitem que os computadores desenvolvam a capacidade de autoaprendizagem, através de treinamentos previamente realizados. A aplicação desse tipo de tecnologia pode ser encontrada por exemplo, na função de desbloqueio facial dos smartphones, onde após um rápido treinamento o dispositivo é habilitado para somente liberar acesso do celular após o reconhecimento da face do usuário do aparelho. Este tipo de tecnologia funciona através do reconhecimento de imagens, baseado em inteligência artificial e aprendizado de máquina. Este artigo tem como objetivo, realizar a comparação de dois métodos distintos de análise morfológica das nanofibras, sendo eles a análise analógica através do software ImageJ e a análise computacional através de inteligência artificial com base de aprendizagem de máquina utilizando redes neurais (metodologia desenvolvida pelo grupo, através da adaptação de um algoritmo de reconhecimento de imagens). Como hipótese o artigo avalia se a identificação da presença de beads e o número de beads pelo método analógico é estatisticamente semelhante (HO) ou estatisticamente diferente (H1) ao método utilizando aprendizagem de máquina. As imagens utilizadas foram retiradas por meio de um MEV e posteriormente analisadas utilizando dois diferentes métodos, o resultado dessas análises comprovaram uma eficiência superior do método computacional, por ser mais rápido. No entanto, foi identificado uma baixa precisão, uma vez que os resultados do primeiro teste da acurácia do método computacional não foram satisfatórios. Por conta disso, ações foram tomadas a fim de aumentar a confiabilidade do método computacional.

    Palavras-Chave: nanofibers; artificial intelligence; morphology; computers

    Como referenciar este item
    Icon

  • IPEN-DOC 29452

    ALVES, V.M. ; LUGAO, A.B. ; FERREIRA, A.H. ; CRUZ, C.P. . Nanopartículas proteicas: síntese induzida por radiação ionizante em substituição aos métodos convencionais. In: CONGRESSO BRASILEIRO DE ENGENHARIA E CIÊNCIA DOS MATERIAIS, 24., 6-10 de novembro, 2022, Águas de Lindóia, SP. Resumo... 2022.

    Abstract: Nanopartículas de albumina estão sendo utilizadas para diferentes tipos de aplicações principalmente na área farmacêutica como carreadores de drogas e ou radionuclídeos. A albumina pertence a um importante grupo de materiais empregados em nanoescala com propriedades essenciais como tamanho ideal de partícula, alta biocompatibilidade, baixa toxicidade e facilidade de alteração da superfície entre outras características. Comercialmente estão disponíveis dois produtos: Abraxane® (paclitaxel ligado à albumina) e Nanocoll® (reagente liofilizado para detecção de linfonodo sentinela quando radiomarcado). Recentemente, o uso de radiação como rota alternativa para promover a reticulação de partículas proteicas tem sido amplamente discutido e tem demonstrado a possibilidade de preservação das estruturas proteicas e manutenção de suas atividades biológicas. Além disso, a síntese assistida por radiação mostrou-se eficaz no controle de tamanho e diminuição da toxicidade pela ausência de reticulantes químicos. Os efeitos da irradiação de proteínas, especialmente em solução aquosa, envolvem a geração de espécies reativas proveniente da radiólise da água ou solvente, que são capazes de promover a reticulação. Na busca por uma rota otimizada e melhor entendimento sobre o uso da radiação na promoção da reticulação proteica esse trabalho propôs estudo das condições de síntese de nanopartícula de albumina por irradiação com elétrons (Eletron beam). Foram realizadas sínteses nas concentrações proteicas 0,5, 2,5, 5, 7,5 e 10 mg/mL, com 30% de etanol (v/v), em dois tampões distintos: fosfato (50mM) e tris-HCl (50mM), todos em atmosfera de NO. Posteriormente as amostras foram submetidas ao processo de irradiação com elétrons variando a dose 1 a 25 KGy e avaliadas em relação ao raio hidrodinâmico em solução e agregação das nanopartículas, usando a técnica de DLS. As análises estatísticas mostraram que o tampão tris-HCl está associado com maiores valores para o tamanho da nanopartícula do que o tampão fosfato (aproximadamente 2 vezes). A concentração de albumina não apresenta uma associação clara com o diâmetro, diferentemente da irradiação, que mostra uma leve associação positiva, ao aumentar a dose de irradiação em um kGy, se espera um aumento de aproximadamente 4% no diâmetro hidrodinâmico. E em relação ao aumento da concentração de albumina em um mg/mL, espera-se uma diminuição de aproximadamente 2% no diâmetro hidrodinâmico.

    Palavras-Chave: nanoparticles; albumins; electron beams; radiolysis

    Como referenciar este item
    Icon

  • IPEN-DOC 29451

    SILVA, B.M.; TEGON, C.C.; MELO, M.; GONCALVES, R.L.; COUTO, A.A. ; MASSI, M.. Desenvolvimento e caracterização de implantes para osteossíntese, constituídos por nanocompósitos de matriz polimérica e óxido de grafeno. In: CONGRESSO BRASILEIRO DE ENGENHARIA E CIÊNCIA DOS MATERIAIS, 24., 6-10 de novembro, 2022, Águas de Lindóia, SP. Resumo... 2022.

    Abstract: Neste trabalho foram estudadas as propriedades químicas e térmicas de nanocompósitos de polietileno de baixa densidade (PEBD) reforçados com óxido de grafeno para confecção de implantes via impressão 3D. O processamento do nanocompósito foi realizado por processo de extrusão em uma extrusora mono rosca e foram fabricados nanocompósitos com diferentes cargas de óxido de grafeno. Após essa etapa, foram construídos filamentos para testes em impressora 3D convencional. Foram realizadas caracterizações químicas e térmicas de cada nanocompósito produzido, inclusive PEBD sem GO. Para a caracterização química, foi utilizada a técnica de espectroscopia no infravermelho por transformada de Fourier (FTIR), além das fases presentes pela técnica de Difração de Raios X (DRX). Para as análises térmicas, foi utilizada a técnica de Termogravimetria (TGA). Os resultados obtidos demonstraram que a incorporação do óxido de grafeno (GO) na matriz polimérica, pode promover maior adesão celular, além de aumento na cristalinidade e de temperatura de perda de massa do nanocompósito. Os filamentos impressos, se apresentaram como termicamente estáveis, porém ainda é necessário um estudo visando os parâmetros de impressão.

    Como referenciar este item
    Icon

  • IPEN-DOC 29450

    BARBOSA, I.T.; BALIZA, P.C.; ROCHA, G.L.; MIRANDA, L.F.; MUNHOZ JUNIOR, A.H.; SILVA, L.G. ; OLIVEIRA, B.E.. Caracterização de hidrogéis contendo nanoemulsões de ácido mandélico e diferentes óleos essenciais. In: CONGRESSO BRASILEIRO DE ENGENHARIA E CIÊNCIA DOS MATERIAIS, 24., 6-10 de novembro, 2022, Águas de Lindóia, SP. Resumo... 2022.

    Abstract: Os hidrogéis são matrizes poliméricas de liberação de ativos, com toxicidade mínima, consistência elastomérica e alta permeabilidade. Estes produtos possuem excelentes aplicações na área cosmética como veículos de fármacos. Neste trabalho foram preparados hidrogéis com concentrações de PVP (7,5% em massa), PEG (3% em massa) e ágar (1% em massa) contendo pseudoboemita, ácido mandélico com óleo essencial de Palmarosa, Lavanda, Gerânio e/ou Lemongrass, submetidos a radiação de 25 kGy. Os hidrogéis foram caracterizados através de análise sensorial, desidratação isotérmica com arraste de ar e desidratação em função do tempo. Os resultados obtidos mostraram que os hidrogéis contendo diferentes óleos essenciais sofrem uma desidratação em função do tempo na ordem de 2% a 4%; enquanto o hidrogel contendo óleo essencial de Lavanda apresentou 23% desidratação isotérmica com arraste de ar. Quanto ao grau de satisfação, o hidrogel com óleo essencial de Lemongrass foi o mais adequado para o mercado consumidor. Portanto, conclui-se que essas análises possuem relevância para o desenvolvimento de um cosmético.

    Como referenciar este item
    Icon

  • IPEN-DOC 29449

    NOGUEIRA, K.M. ; VARCA, J.O. ; LIMA, C.S. ; CRUZ, C.C. da ; RIBEIRO, A.H. ; FREITAS, L.F. ; VARCA, G.H. ; LUGAO, A.B. . Solubility study of Kraft lignin for the development of electrospun nanofibers. In: CONGRESSO BRASILEIRO DE ENGENHARIA E CIÊNCIA DOS MATERIAIS, 24., 6-10 de novembro, 2022, Águas de Lindóia, SP. Resumo... 2022.

    Abstract: Lignin is a high-volume byproduct of paper manufacturing which has been explored in many research fields, especially for the development of fiber and nanofiber for biomedical applications [1,2]. This work presents a solubility study performed through gravimetry for kraft lignin considering its application for the development of electrospun nanofibers [3]. In practical terms, lignin was solubilized in alkaline aqueous solution, dimethylformamide and dimethylsulfoxide, at concentrations of 10, 15 and 20% (w/v) and varying temperatures of 25, 50 and 75 ºC, under constant stirring. After solubilizing, the solution was filtered, and the insoluble fraction was dried in the oven at 100 ºC. At 25 ºC lignin was insoluble in all solvents tested, as predicted using Hansen solubility parameters. Although the increase in temperature promoted lignin solubilization in all solvents tested, at the highest temperature assayed, the solubilization was facilitated, presenting the smallest levels of the insoluble fraction. Lignin was soluble in all solvents tested, and optimum solubility conditions were achieved using 10% lignin solutions (w/v), without significant insoluble fraction, and therefore ideal concentration for the development of lignin based fibers.

    Como referenciar este item
    Icon

  • IPEN-DOC 29448

    VARCA, J.O. ; KLINGBEIL, F. ; NOGUEIRA, K.M. ; LIMA, C.S. ; CRUZ, C.C. da ; FREITAS, L.F. ; VARCA, G.H. ; MATHOR, M.B. ; LUGAO, A.B. . A biological study of gelatin-PVA based scaffold functionalized with albumin for biomedical purposes. In: CONGRESSO BRASILEIRO DE ENGENHARIA E CIÊNCIA DOS MATERIAIS, 24., 6-10 de novembro, 2022, Águas de Lindóia, SP. Resumo... 2022.

    Abstract: Biomaterials have been designed for tissue reconstruction, bone regeneration and cell culture, and functionalized with presence of proteins, nanoparticles, peptides and other components to improve the biocompatibility for instance. This work shows a biological study of gelatin-PVA based scaffold with controlled pore size and functionalized with albumin for biomedical purposes. The in vitro study comprises cytotoxicity, cell adhesion and proliferation assessment. In practical terms, the gelatin-PVA scaffold crosslinked and sterilized by gamma radiation followed by freeze-drying was evaluated by cytotoxicity, adhesion and proliferation tests. The cytotoxicity results showed that the biomaterial produced was non-toxic, and adhesion and proliferation assays showed that the material was suitable for tissue engineering. The presence of albumin did not present a significant impact on the cell performance, at the assayed concentration.

    Como referenciar este item
    Icon

  • IPEN-DOC 29447

    ALVES, J.A. ; MUCSI, C.S. ; ROSSI, J.L. ; KANAYAMA, P.H.. An evaluation of the utilization of solar power generation both ac and dc to enhance life quality of insulated communities. In: CONGRESSO BRASILEIRO DE ENGENHARIA E CIÊNCIA DOS MATERIAIS, 24., 6-10 de novembro, 2022, Águas de Lindóia, SP. Resumo... 2022.

    Abstract: The electric power availability for insulated communities both geographically and after major disasters is a recurrent problem throughout the world even in modern days. Nowadays the availability of silicon solar electric generating panels is widely available in a wide range of quality, translated into prices, as well as all the ancillaries needed to put up a solar power generating system in operation. Solar panels generate direct current that may charge batteries or be converted to alternating current both with its characteristic circuit losses; usually DC to AC conversion present losses and generate harmonics that may be inserted into sensitive common domestic appliances like radios; even so, most electronic gear usually reconvert AC to DC for its operation. So choosing the right powering system may provide a less expensive and more efficient way to provide quality of life for insulated communities. This paper present preliminary results on the comparison between a DC and an AC systems for the powering of usual domestic appliances as well as report a former experiment to evaluate the effect of time on the perception of the users and the concerns associated. Two power-generating systems were conceived and installed in a habitation, one of then for DC generation and the other an AC generation system. Both systems were aimed to power equivalent loads and equipment. A field visit was made to an insulated community in order to collect data about the usability, concerns and operation of a photovoltaic system offered to them some time ago. Results of the comparison between AC and DC powering systems are mixed; the DC system had a low initial price but the appliances are more expensive and not readily available and its maintenance more difficult. Otherwise the AC powering system behaves in the opposite direction. The choice is solely based in the availability of appliances and ancillaries and financing options.

    Como referenciar este item
    Icon

  • IPEN-DOC 29446

    LEITE, A.C.; SANTOS, G.W. dos; VASCONCELLOS, L.A.; SILVA, S.P. da; FERRUS FILHO, A.; PELARIN, A.L.; BENTO, R.T. . Desenvolvimento de um adaptador de tração motorizado para cadeiras de rodas. In: CONGRESSO BRASILEIRO DE ENGENHARIA E CIÊNCIA DOS MATERIAIS, 24., 6-10 de novembro, 2022, Águas de Lindóia, SP. Resumo... 2022.

    Abstract: No Brasil, atualmente cerca de 46 milhões de pessoas apresentam algum tipo de deficiência, sendo que 7 % correspondem a população que possui algum déficit de mobilidade motora e necessitam de auxílio de cadeiras de rodas para se locomover, e 2,3 % exibem deficiência motora severa. Embora os atuais modelos de cadeiras de rodas facilitem a mobilidade de seus usuários, a funcionalidade e o trabalho realizado durante o seu uso ainda são um desafio desgastante para situações cotidianas, tais como subidas acentuadas, calçadas desniveladas, e esforço extremo dos membros superiores, por exemplo. Tendo em vista as respectivas dificuldades, diversas pesquisas vêm sendo desenvolvidas a fim de propor soluções e garantir os direitos civis de mobilidade segura, eficiente e independente aos cadeirantes. A proposta do presente trabalho é desenvolver um adaptador de tração motorizado que pode ser acoplado em qualquer modelo de cadeira de rodas, a fim de auxiliar a reduzir o esforço mecânico do usuário. O projeto atua na área de mobilidade reduzida, visando melhorar a locomoção dos cadeirantes. O sistema empregado para essa corretiva de mobilidade é do tipo plug and play, facilitando assim a utilização do produto. O acoplamento é formado por um conjunto eletromecânico constituído por roda, guidão e garfo, que, ao se encaixar na cadeira de rodas, a transforma em um triciclo elétrico, promovendo maior autonomia, conforto e fácil manuseio, atendendo às necessidades dos usuários. O protótipo foi desenvolvido totalmente de forma artesanal, sua modelagem foi projetada no software SolidWorks, no qual cada peça do adaptador foi simulada considerando diferentes materiais: aço 1045, alumínio 6063-T6 e aço 4340, a fim de avaliar o melhor material para composição do produto. O modelamento e análise estrutural estática pelo Método de Elementos Finitos permitiu concluir que, a partir dos valores de tensão máxima de Von Misés encontradas nas simulações para cada um dos materiais propostos, as peças projetadas suportarão os esforços aos quais elas serão exigidas ao durante sua utilização prática, uma vez que em nenhuma das simulações houve qualquer deformação crítica dos componentes, todos os valores de tensão encontram-se abaixo da tensão de escoamento dos materiais e com coeficientes de segurança extremamente aceitáveis. Com a construção do protótipo funcional do adaptador de tração, foi possível concluir que o desenvolvimento do presente projeto promoveu a criação de um dispositivo mecânico funcional e inovador, proporcionando a inclusão desse grupo à sociedade, visando facilitar o seu conforto e sua mobilidade urbana, a partir da busca por um projeto de menor custo para atender também as classes mais carentes financeiramente. O trabalho merece destaque, por buscar apresentar um projeto de caráter tecnológico, científico e social.

    Como referenciar este item
    Icon

A pesquisa no RD utiliza os recursos de busca da maioria das bases de dados. No entanto algumas dicas podem auxiliar para obter um resultado mais pertinente.

✔ É possível efetuar a busca de um autor ou um termo em todo o RD, por meio do Buscar no Repositório , isto é, o termo solicitado será localizado em qualquer campo do RD. No entanto esse tipo de pesquisa não é recomendada a não ser que se deseje um resultado amplo e generalizado.

✔ A pesquisa apresentará melhor resultado selecionando um dos filtros disponíveis em Navegar

✔ Os filtros disponíveis em Navegar tais como: Coleções, Ano de publicação, Títulos, Assuntos, Autores, Revista, Tipo de publicação são autoexplicativos. O filtro, Autores IPEN apresenta uma relação com os autores vinculados ao IPEN; o ID Autor IPEN diz respeito ao número único de identificação de cada autor constante no RD e sob o qual estão agrupados todos os seus trabalhos independente das variáveis do seu nome; Tipo de acesso diz respeito à acessibilidade do documento, isto é , sujeito as leis de direitos autorais, ID RT apresenta a relação dos relatórios técnicos, restritos para consulta das comunidades indicadas.

A opção Busca avançada utiliza os conectores da lógica boleana, é o melhor recurso para combinar chaves de busca e obter documentos relevantes à sua pesquisa, utilize os filtros apresentados na caixa de seleção para refinar o resultado de busca. Pode-se adicionar vários filtros a uma mesma busca.

Exemplo:

Buscar os artigos apresentados em um evento internacional de 2015, sobre loss of coolant, do autor Maprelian.

Autor: Maprelian

Título: loss of coolant

Tipo de publicação: Texto completo de evento

Ano de publicação: 2015

✔ Para indexação dos documentos é utilizado o Thesaurus do INIS, especializado na área nuclear e utilizado em todos os países membros da International Atomic Energy Agency – IAEA , por esse motivo, utilize os termos de busca de assunto em inglês; isto não exclui a busca livre por palavras, apenas o resultado pode não ser tão relevante ou pertinente.

✔ 95% do RD apresenta o texto completo do documento com livre acesso, para aqueles que apresentam o significa que e o documento está sujeito as leis de direitos autorais, solicita-se nesses casos contatar a Biblioteca do IPEN, bibl@ipen.br .

✔ Ao efetuar a busca por um autor o RD apresentará uma relação de todos os trabalhos depositados no RD. No lado direito da tela são apresentados os coautores com o número de trabalhos produzidos em conjunto bem como os assuntos abordados e os respectivos anos de publicação agrupados.

✔ O RD disponibiliza um quadro estatístico de produtividade, onde é possível visualizar o número dos trabalhos agrupados por tipo de coleção, a medida que estão sendo depositados no RD.

✔ Na página inicial nas referências são sinalizados todos os autores IPEN, ao clicar nesse símbolo será aberta uma nova página correspondente à aquele autor – trata-se da página do pesquisador.

✔ Na página do pesquisador, é possível verificar, as variações do nome, a relação de todos os trabalhos com texto completo bem como um quadro resumo numérico; há links para o Currículo Lattes e o Google Acadêmico ( quando esse for informado).

ATENÇÃO!

ESTE TEXTO "AJUDA" ESTÁ SUJEITO A ATUALIZAÇÕES CONSTANTES, A MEDIDA QUE NOVAS FUNCIONALIDADES E RECURSOS DE BUSCA FOREM SENDO DESENVOLVIDOS PELAS EQUIPES DA BIBLIOTECA E DA INFORMÁTICA.

O gerenciamento do Repositório está a cargo da Biblioteca do IPEN. Constam neste RI, até o presente momento 20.950 itens que tanto podem ser artigos de periódicos ou de eventos nacionais e internacionais, dissertações e teses, livros, capítulo de livros e relatórios técnicos. Para participar do RI-IPEN é necessário que pelo menos um dos autores tenha vínculo acadêmico ou funcional com o Instituto. Nesta primeira etapa de funcionamento do RI, a coleta das publicações é realizada periodicamente pela equipe da Biblioteca do IPEN, extraindo os dados das bases internacionais tais como a Web of Science, Scopus, INIS, SciElo além de verificar o Currículo Lattes. O RI-IPEN apresenta também um aspecto inovador no seu funcionamento. Por meio de metadados específicos ele está vinculado ao sistema de gerenciamento das atividades do Plano Diretor anual do IPEN (SIGEPI). Com o objetivo de fornecer dados numéricos para a elaboração dos indicadores da Produção Cientifica Institucional, disponibiliza uma tabela estatística registrando em tempo real a inserção de novos itens. Foi criado um metadado que contém um número único para cada integrante da comunidade científica do IPEN. Esse metadado se transformou em um filtro que ao ser acionado apresenta todos os trabalhos de um determinado autor independente das variáveis na forma de citação do seu nome.

A elaboração do projeto do RI do IPEN foi iniciado em novembro de 2013, colocado em operação interna em julho de 2014 e disponibilizado na Internet em junho de 2015. Utiliza o software livre Dspace, desenvolvido pelo Massachusetts Institute of Technology (MIT). Para descrição dos metadados adota o padrão Dublin Core. É compatível com o Protocolo de Arquivos Abertos (OAI) permitindo interoperabilidade com repositórios de âmbito nacional e internacional.

1. Portaria IPEN-CNEN/SP nº 387, que estabeleceu os princípios que nortearam a criação do RDI, clique aqui.


2. A experiência do Instituto de Pesquisas Energéticas e Nucleares (IPEN-CNEN/SP) na criação de um Repositório Digital Institucional – RDI, clique aqui.

O Repositório Digital do IPEN é um equipamento institucional de acesso aberto, criado com o objetivo de reunir, preservar, disponibilizar e conferir maior visibilidade à Produção Científica publicada pelo Instituto, desde sua criação em 1956.

Operando, inicialmente como uma base de dados referencial o Repositório foi disponibilizado na atual plataforma, em junho de 2015. No Repositório está disponível o acesso ao conteúdo digital de artigos de periódicos, eventos, nacionais e internacionais, livros, capítulos, dissertações, teses e relatórios técnicos.

A elaboração do projeto do RI do IPEN foi iniciado em novembro de 2013, colocado em operação interna em julho de 2014 e disponibilizado na Internet em junho de 2015. Utiliza o software livre Dspace, desenvolvido pelo Massachusetts Institute of Technology (MIT). Para descrição dos metadados adota o padrão Dublin Core. É compatível com o Protocolo de Arquivos Abertos (OAI) permitindo interoperabilidade com repositórios de âmbito nacional e internacional.

O gerenciamento do Repositório está a cargo da Biblioteca do IPEN. Constam neste RI, até o presente momento 20.950 itens que tanto podem ser artigos de periódicos ou de eventos nacionais e internacionais, dissertações e teses, livros, capítulo de livros e relatórios técnicos. Para participar do RI-IPEN é necessário que pelo menos um dos autores tenha vínculo acadêmico ou funcional com o Instituto. Nesta primeira etapa de funcionamento do RI, a coleta das publicações é realizada periodicamente pela equipe da Biblioteca do IPEN, extraindo os dados das bases internacionais tais como a Web of Science, Scopus, INIS, SciElo além de verificar o Currículo Lattes. O RI-IPEN apresenta também um aspecto inovador no seu funcionamento. Por meio de metadados específicos ele está vinculado ao sistema de gerenciamento das atividades do Plano Diretor anual do IPEN (SIGEPI). Com o objetivo de fornecer dados numéricos para a elaboração dos indicadores da Produção Cientifica Institucional, disponibiliza uma tabela estatística registrando em tempo real a inserção de novos itens. Foi criado um metadado que contém um número único para cada integrante da comunidade científica do IPEN. Esse metadado se transformou em um filtro que ao ser acionado apresenta todos os trabalhos de um determinado autor independente das variáveis na forma de citação do seu nome.